2006 Annual Report

2006 Annual Report Life Sciences Education and Research @ Our Growing Commitment The Biotechnology Research Center (BRC) continues its steady pace of growth with a high level of scholarly productivity. Faculty membership has grown to twenty-two and spans three Colleges/School and seven Departments. Research projects at the BRC are funded by all major federal agencies, including DOE, EPA, NIH, NSF, USDA and various foundations, totaling over $10 million. BRC research expenditures were also on the rise, totaling over $2.3 million in FY06. Together, BRC faculty supported or otherwise mentored more than 59 graduate students, 58 undergraduate assistants and 18 research scientists/associates - an example of the faculty’s strong commitment to the Center’s mission of strengthening life sciences research and education at Michigan Tech. BRC also sponsored 10 graduate student and postdoc Travel Awards during the past year, and co-sponsored the second Annual Graduate Student Research Forum, presenting 3 BRC Poster Awards. The BRC faculty hosted its first annual Biotechnology Open House in January 2006, with 26 posters showcasing various research activities and promoting research interactions across the campus community. In addition, a 2-year initiative for infrastructure improvement has been launched, and so far BRC has committed $47K in matches toward equipment and software purchases valuing over $153K. Such investment is expected to strengthen biotechnology infrastructure and to have a broad impact across campus. Sincerely yours, BRC 2006 at a Glance Chung-Jui Tsai Director 22 Faculty members 42 Ph.D. students 17 M.S. students 58 Undergraduate students 18 Research scientists/associates 55 Peer-reviewed publications 42 Active research projects $2,515,391 New research funding $10,577,242 Total research funding $2,382,084 Total research expenditures Images of directed neuronal regeneration on aligned polymer fibers (top) and blood capillaries (bottom), taken with the Apotome microscope purchased with matching funds from a BRC seed grant. Photo courtesy of Ryan Gilbert and Jeremy Goldman. -2- Susan Bagley Professor Biological Sciences microbial degradation and decomposition microbial control toxicology of environmental contaminants Victor Busov Assistant Professor Forest Resources & Environmental Science phytohormones activation tagging forward genetics and genomics Huann-Sheng Chen Associate Professor Mathematical Sciences statistics statistical genetics Seth Donahue Associate Professor Biomedical Engineering bone tissue engineering bone cell mechanotransduction osteoporosis Ryan Gilbert Assistant Professor Biomedical Engineering nerve regeneration using polymer fibers cartilage tissue engineering neural tissue engineering with hydrogels Jeremy Goldman Assistant Professor Biomedical Engineering Faculty vascular remodeling lymphangiogenesis -3- Michael Gretz Professor Biological Sciences algal extracellular polymers cell wall polysaccharides biogenesis of biopolymers Scott Harding Research Associate Professor Forest Resources & Environmental Science carbon allocation and partitioning secondary metabolism molecular physiology Tammy Haut Donahue Associate Professor Mechanical Engineering-Engineering Mechanics meniscus mechano-biology orthopaedic biomechanics computational mechanics Patricia Heiden Associate Professor Chemistry bio- and nanocomposites and biomaterials nanoparticles, nanofibers & controlled release environmentally benign synthesis & processing Chandrashekhar Joshi Associate Professor Forest Resources & Environmental Science cellulose and lignin biosynthesis wood development and tree growth bioinformatics and genomics Haiying Liu Assistant Professor Chemistry Faculty bacterial biosensors carbon and boron nitride nanotube arrays fluorescent conjugated glycopolymers -4- Pushpalatha Murthy Professor Chemistry signal transduction in plants Inositol phosphate metabolism molecular biology of phytases Keat Ghee Ong Assistant Professor Biomedical Engineering implantable biosensors medical devices wireless sensors Rupak Rajachar Assistant Professor Biomedical Engineering cell-instructive materials vascular calcification cell mechanotransduction David Shonnard Professor Chemical Engineering enzyme directed evolution metabolic engineering environmental life cycle assessment Martin Thompson Assistant Professor Chemistry post-translational modification of proteins acetylation dependence of gene regulation histone-protein interactions Chung-Jui Tsai Director and Professor Forest Resources & Environmental Science Faculty phenylpropanoid metabolism microarray and metabolite profiling cytoskeleton and wood formation -5- Leah Vucetich Research Assistant Professor Forest Resources & Environmental Science conservation genetics molecular genotyping Isle Royale population dynamics Ramakrishna Wusirika Assistant Professor Biological Sciences gene regulation by transposable elements evolution of plant disease resistance genes bidirectional promoters Heather Youngs Assistant Professor Biological Sciences plant biochemistry protein engineering peptidomimetics Shuanglin Zhang Associate Professor Mathematical Sciences statistics statistical genetics bioinformatics Mary Tassava Staff Assistant Forest Resources and Environmental Science Faculty -6- Education and Training The BRC continued its strong commitment to life sciences education by offering research experiences and financial support to more than 59 graduate students and 58 undergraduate students in BRC-affiliated laboratories during 2006. The BRC also continued to reach out to the K-12 community through various outreach activities, as well as to the post-graduate (B.S., M.S., and Ph.D.) community for continuing research, training and career advancement. High School Students Meha Pandey, Houghton High School (Gilbert) Brandi Bailey, Timbuktu Academy, LA (Haut Donahue) Ebenezer Omojola, Timbuktu Academy, LA (Haut Donahue) NSF-sponsored Bioinformatics and Test-tube Gardening Summer Youth Programs (Tsai) Kevin Bausley Marc Brown Matthew Griffith Bianca Kincannon Ashley McBride Allan McDaniel Danielle Nelson Rutisha Warren Marc Brown Molly Brown Constance Carlson Corinne Doezema Ian-Michael Flake Terrence Martin Carlos Moore Courtney Pack Aleesa Schlientz Poster presentation by SYP students Undergraduates (58 students) Lindsay Barlow, Biomed (Donahue) Benjamin Baer, Chemistry (Tsai) Richard Bolander, Biomed (Haut Donahue) Holly Burger, Biology (Gretz) Owen Branson, Chemistry (Murthy) Thomas Brigg, ECE (Harding) Marcus Carli, Biology (Gretz) Andrew Delvaux, Biomed (Gilbert) Stephanie Drake, Biomed (Tsai) Megan Drelles, Biomed (Goldman) Josh Dykla, Biomed (Gilbert) Jessey Erath, Biology (Bagley) Marcy Erickson, Applied Ecology (Vucetich) Sara Gibbs, Biomed (Goldman) Idaliza Gomez, Biology (Tsai) Julie Goral, Biomed (Goldman) Jesse Hansen, Engineering (Harding) Tara Hansen, Biology (Haut Donahue) Tendeukai Hungwe, Biology (Gretz) Samantha Howell, Applied Ecology (Vucetich) Brian Hunter, Applied Ecology (Busov) Logan Janka, Biomed (Rajachar) Rebecca Klank, Biomed (Gilbert) Laura Kluskens, ME-EM (Joshi) Thomas Kohlman, Engineering (Busov) Christopher Kupitz, Chemistry (Thompson) Katie Kruger, Biology (Wusirika/Joshi) Marie Kate Lanczy, Biology (Bagley) Andrew Maday, Forestry (Busov) Keith Magic, Biomed (Donahue) Aaron Maki, Biomed (Donahue) Benton Martin, Biomed (Gilbert) Katie McKeown, Biomed (Goldman) Rosemarie Morrison, Applied Ecology (Tsai) Louis Pallidino, Biology (Bagley) Joe Quello, Biology (Bagley) Alisha Raehl, non-degree seeking (Goldman) Jill Recla, Bioinformatics (Busov) Michelle Riccio, Biology (Gretz) Chris Rivet, Biomed (Gilbert) Sorangel Rodríguez, Chemistry (Liu) Kacie Scholl, Biology (Bagley) Emily Smith, Biology (Bagley) Samantha Stam, Chemistry (Thompson) Hwee Chi Tay, Biology (Joshi, Youngs) David Tefft, Biomed (Gilbert) Chelsea Thomas, Applied Ecology (Vucetich) Matthew Trombley, Biomed (Gilbert) Emily Umlor, Biology (Tsai) Joseph Uzarski, Biomed (Goldman) Jon Van Regenmorter, ChemEngg (Shonnard) Jeffrey Vorhies, ChemEngg (Shonnard) Shu Wei, Engineering (Busov) Kelli Whelan, Chemistry (Thompson) Justine Wiles, ME-EM (Tsai) Marie Wikening, Bioinformatics (Thompson, Busov) Sherri Wiseman, Biomed (Gilbert) Kassidy Yatso, Applied Ecology (Tsai) -7- Summer Undergraduate Interns and Fellowships Alan Berger, Department of Applied Science, Pennsylvania State University (Shonnard) Stephanie Drake, MTU-SURF Fellowship, Biomedical Engineering (Tsai) Idaliza Gomez, NSF-REU Fellowship, Biological Sciences (Tsai) Tara Hansen, MTU-SURF Fellowship, Biological Sciences (Haut Donahue) Katie Kruger, MTU-SURF Fellowship, Biological Sciences (Wusirika) Mehreteab Mengisteab, NSF-REU Fellowship, Grand Rapids Community College (Tsai) Jill Recla, MTU-SURF, Bioinformatics (Busov) Hwee Chi Tay, MTU-SURF Fellowship, Biology (Youngs) Graduate Students (42 Ph.D. and 17 M.S. students) Biological Sciences Abass Abdullahi (Ph.D., Gretz, graduated) Melba Apoya (Ph.D., Gretz) Brent Bellinger (Ph.D., Gretz, graduated) Robert Donofrio (Ph.D., Bagley) Sara Kiemle (Ph.D., Gretz) Nicholas Krom (Ph.D., Wusirika) Maara Paackalen (M.S., Bagley) Ratul Saha (M.S., Bagley, graduated) Ratul Saha (Ph.D., Bagley) Daryll Yarger (M.S., Bagley) Zijun Xu (Ph.D., Wusirika) Biomedical Engineering Matt Barron (Ph.D., Donahue) Rachel Bradford (Ph.D., Donahue) Sarah Galley (Ph.D., Donahue, graduated) Meghan McGee (Ph.D., Donahue) Eric Minner (Ph.D, Gilbert) Hanbing Wang (Ph.D., Gilbert) Chemical Engineering Michael Brodeur Campbell (Ph.D., Shonnard) Abraham Martin-Garcia (Ph.D., Shonnard, graduated) Jill Jensen (Ph.D., Shonnard) Shu Chiang Yat (M.S., Shonnard, graduated) Shu Chiang Yat (Ph.D., Shonnard) Chemistry Feifei Cai (M.S., Liu) Renu Chandrasekaran (Ph.D.,Thompson) Zhen Chen (Ph.D., Liu) Venkat R. Donuru (Ph.D., Liu) Qi Gao (Ph.D., Liu) Steve Johnson (Ph.D., Murthy) Singaravelu Velayudham (Ph.D., Liu) Tara Hansen dissecting a knee joint. Mathematical Sciences Xiao Lu (Ph.D., Chen) Xiaoqi Cui (Ph.D., Chen Sapna Kumari (M.S., Chen) Li Li (M.S., Chen, graduated) Fei Wang, (Ph.D., Chen) Shijuan Wang (M.S., Chen, graduated) Forest Molecular Genetics & Biotechnology Edward Anino (Ph.D., Tsai/Harding) Pushpinder Brar (Ph.D., Joshi) Fanny Casado (M.S., Tsai/Harding, graduated) Han-Yi Chen (Ph.D., Tsai) Trisha Colling (M.S., Busov) Chamini Geethenjalee (Ph.D.,Busov) Asha Lakkavaram (M.S., Joshi, graduated) Rajinikanth Mohan (Ph.D., Tsai, graduated) Rodney Oakley (Ph.D., Tsai) Raja S. Payyavula (Ph.D., Tsai/Harding) Rewati Potkar (M.S., Busov, graduated) Shiv Thammannagowda (Ph.D., Joshi) Siau Sie Voo (M.S., Joshi, graduated) Melissa Watkins (M.S., Vucetich) Fuyu Xu (Ph.D., Joshi) Jingwei Yin (Ph.D., Tsai/Harding) Christine Zawaski (Ph.D., Busov) Mechanical Engg-Engg Mechanics Cris Braganza (M.S., Donahue, graduated) Tumul Gupta (Ph.D., Haut Donahue) Robert Lemon (M.S.., Haut Donahue) Duane Morrow (Ph.D., Haut Donahue) Michael Steele (M.S., Haut Donahue) Diego Villegas (Ph.D., Haut Donahue) Basia Zielinska (Ph.D., Haut Donahue) Research Scientists/Associates (11 Ph.D. scientists and 7 associates) David W. Caspary, Laboratory Manager (Shonnard) Dr. Ketan Doshi, Postdoctoral Scientist (Busov) Dr. Walid El Kayal, Postdoctoral Scientist (Harding) Dr. Takeshi Fujino, Postdoctoral Scientist (Joshi) Dr. Jiqing Gou, Postdoctoral Scientist (Busov) Dr. Changyu Hu, Postdoctoral Scientist (Tsai) Sandra Hubscher, Research Associate (Tsai) Michelle Jarvie, Research Associate (Tsai/Harding) Rama Joshi, Research Associate (Busov) Mahita Kadmiel, Research Associate (Busov) Dr. Han-Wei Lin, Postdoctoral Scientist (Tsai) Dr. Yunxia Liu, Postdoctoral Scientist (Joshi) Matt Nelsen, Research Associate (BRC) Dr. Priya Ranjan, Postdoctoral Scientist (Tsai) Dr. Alexander Raskind, Postdoctoral Scientist (Harding) Karena Schmidt, Research Associate (Vucetich) Dr. Yinan Yuan, Postdoctoral Scientist (Tsai) Dr. Cuihua Xue, Postdoctoral Scientist (Liu) -8- Honors Tara Hansen (Biology, Haut Donahue), received third place in the Undergraduate Student Paper Competition of the American Society of Mechanical Engineers Summer Conference, and a Michigan Space Grant Consortium award. Shu Chiang Yat (ChemEngg, Shonnard), received a first place award in the MTU Graduate Student Council Student Poster Competition. Marcy Erickson (FRES, Vucetich), received $1,200 summer funds from Kimberly Clark though Educational Opportunities. Edward Anino (FRES, Tsai), received a Society for In Vitro Biology John S. Song Travel Award to present a poster at the SIVB Annual Meeting in June, 2006. Ratul Saha (Biology, Bagley), received an American Society for Microbiology Student Travel Award to present a poster at the ASM Annual Meeting in May, 2006. Professor Tammy Haut Donahue (ME-EM) received the Ferdinard P. Beer and E. Russell Johnston Jr. Outstanding New Mechanics Educator Award from the American Society for Engineering Educators in 2006. Fuyu Xu (FRES, Joshi), received an American Society for Plant Biologists Travel Award to present a poster at the ASPB Annual Meeting in August, 2006. Idaliza Gomez (Biology, Tsai), received a Travel Award from the Federation of American Societies for Experimental Biology-Minority Access to Research Careers Program to present a poster at the Society for Advancement of Chicanos and Native Americans in Science (SACNAS) conference in November, 2006. ESC/BRC Graduate Research Forum The Ecosystem Science Center and the Biotechnology Research Center co-sponsored the second Annual Graduate Research Forum in February, 2006. Sixteen BRC graduate students submitted abstracts and posters highlighting their research. The 2006 BRC poster award recipients were: $500 Grand Prize: Edward Anino $100 Merit Awards: Rewati Potkar, Mohan Rajinikanth, Shivegowda Thammannagowda BRC 2005-2006 Travel Award Recipients Barbara Zielinska ($250), 2005 Summer Bioengineering Conference; June, 2005 (ME-EM) Sonali Jog ($500), 230th ACS National Meeting; August, 2005 (Chemistry) Lianne Miller ($250), Biomedical Engineering Society Conference; September, 2005 (Biomed) Mohan Rajinikanth ($500) Plant & Animal Genomes Conference; January, 2006 (FRES) Rewati Potkar ($500), 16th Symposium in Plant Physiology; May, 2006 (FRES) Daryll Yarger ($500), 106th Meeting of the ASM; May, 2006 (Biology) Edward Anino ($500), 2006 InVitro Biology Meeting; June, 2006 (FRES) Timul Gupta ($500), 2006 Summer Bioengineering Conference; June, 2006 (ME-EM) Shiv Thammannagowda ($500), 2006 Annual ASPB Meeting; August, 2006 (FRES) Fuyu Xu ($500), 2006 Annual ASPB Meeting; August, 2006 (FRES) Renu Chandrashekaran ($500), 232nd ACS National Meeting; September, 2006 (Chemistry) Abraham Martin Garcia ($500), 2006 AIChE Meeting; November, 2006 (Chem Engg) Shu Chiang Yat ($500), 2006 AIChE Meeting; November, 2006 (Chem Engg) -9- BRC Open House The BRC held its first annual open house in January, 2006 in Hesterberg Hall at the School of Forest Resources and Environmental Science. Faculty, researchers, and students across the campus were invited to an informal poster presentation to learn about the various research activities that are taking place at the BRC. BRC Monthly Seminar The BRC Seminars continued in 2006. The format remains the same: postdocs and graduate students present research results on a rotating basis. The seminars help BRC researchers stay abreast of ongoing research being conducted by other graduate students and postdocs at the Center. The number of BRC-affiliated Departments represented at the Seminar series grew substantially. NIH Proposal Workshop 23 posters were displayed at BRC open house. The BRC helped organize an NIH proposal workshop in December 2006 for researchers from throughout the University to learn about the NIH review process and how the review panel works. Featured speakers included Biomedical Engineering Chair Mike Neuman, an experienced NIH panel member, and BRC faculty member Seth Donahue. Several current NIH PIs were also present to share their experience on preparing NIH proposals. The workshop attracted ~30 faculty and researchers. Presentation materials from the workshop are also available on the BRC website. Invited Speakers The BRC continued to co-sponsor, with its affiliated faculty members and academic units, invited speakers for guest seminars. BRC also helped coordinate several technical seminars and instrument demos with leading scientific vendors. Invited speakers in 2006 included: Dr. Maud Hinchee, Chief Technical Officer of ArborGen visited MTU in January, 2006 and presented a seminar on "Forest biotechnology". Dr. Mark Davis, Senior Research Scientist, National Renewable Energy Laboratory (Adjunct Professor of FRES), presented two seminars in March, 2006: Seminar 1 – “Why is biomass recalcitrant? What physical and chemical properties of pretreated biomass materials make a difference during enzymatic hydrolysis?” Seminar 2: “High throughput screening of biological materials using analytical pyrolysis and multivariate statistical analysis”. Professor David Neale from UC Davis, presented a seminar in April, 2006. The title of his lecture was "Associating natural genetic variation with complex traits in pine”. Dr. Jean Roberts of DOW Agrochemicals visited MTU in June, 2006 and presented a seminar on DOW activities in plant biotechnology area. Professor Hsin-Sheng Tsay, Dean of College of Science and Engineering and Director of Institute of Biotechnology from ChaoYang University of Technology (Taiwan) visited MTU in September, 2006 and presented a seminar on “Commercial application of plant tissue culture technology in Taiwan". Professor Hannah Carey from UW Madison presented a seminar on "Mammalian hibernation: Natural resistance to stress and traumatic injury" in October, 2006. Professor Richard Meilan from Purdue University visited in November, 2006 and presented a seminar on “From Emerald ash borer control to heartwood formation: Molecular tree physiology research at Purdue”. - 10 - Active Research Projects in 2005-2006: 42 grants totaling $10,577,242 in research funds PI, Co-PIs Harding SA, Tsai CJ Busov V Thompson M Wusirika R Tsai CJ Thompson M Tsai CJ, Harding SA Joshi CP, Chiang VL, Li L Gretz M Joshi CP Joshi CP Haut Donahue T, Odegard G Tsai CJ Zhang S Busov V Busov V Chen HS, Zhang S Zhang S Busov V Goldman J Neumann M, Gilbert R, Goldman J Tsai CJ, Harding S Tsai CJ, Harding S Sponsor CPBR MTU-REF MTU-REF MTU-REF USDA-NRI MTU-FSG CPBR Fellowship USDA-NRI NIWAR/NZ CPBR DOW Mayo Clinic Utah State Univ/ USDA-UVB Board NIH CPBR USDA-NRI NIH/HHS NIH ArborGen NIH REF Title Designing promoters for precision targeting of gene expression RNAi in plant development Decode histone code Disease resistant gene cluster Acquisition of a real-time PCR system for quantitative gene expression analysis Identifying chemical determinants of gene regulation using designer proteins Protein engineering for manipulation of phenylpropanoid metabolism Simultaneous expression of angiosperm syringyl monolignol genes in gymnosperm Harmful algae blooms: extracellular polymer production by an invasive diatom in NZ Modulation of cellulose crystallinity in transgenic trees Modulation of cellulose crystallinity in transgenic trees Microsensor for intramuscular pressure measurement Analysis of UV-B response in Populus Statistical methods for mapping complex disease genes Modification of GA metabolism to enhance productivity, wood quality and biosafety Efficiency of activation tagging for functional gene discovery in Populus Mapping complex disease genes incorporating age-at-onset Statistical methods for admixture mapping Modification of GA metabolism to enhance productivity, wood quality and biosafety Augmentation of lymphangiogenesis by increased fluid channeling in mouse skin Acquisition of an ApoTome microscope for three-dimensional fluorescence visualization Protein engineering for manipulation of phenylpropanoid metabolism Protein engineering for manipulation of phenylpropanoid metabolism Funding $126,00 $49,722 $40,000 $40,000 $12,500 $2,550 $51,200 $190,000 $25,000 $43,568 $18,000 $50,000 $10,000 $206,411 $38,358 $342,514 $71,252 $700,029 $12,500 $186,201 $49,782 Duration 01/03 - 12/05 07/04 - 12/05 07/04 - 12/05 07/04 - 12/05 12/04 - 12/05 03/05 - 06/06 06/05 - 08/06 09/02 - 09/06 05/06 - 10/06 01/06 - 04/07 01/06 - 04/07 07/05 - 06/07 09/06 - 06/07 08/05 - 07/07 09/04 - 08/07 09/04 - 08/07 09/04 - 08/07 09/05 - 08/07 11/06 - 08/07 09/06 - 08/07 07/06 - 08/07 CPBR ArborGen $98,001 $30,000 09/06 - 08/07 09/06 - 08/07 - 11 - Research Projects (continued) PI, Co-PIs Joshi CP Tsai CJ Busov V Joshi CP, Chiang VL, Li L Gretz M Tsai CJ, Harding SA Joshi CP Joshi CP Haut Donahue T Joshi CP Haut Donahue T Harding SA, Tsai CJ, Lindroth RL Busov V Sponsor USDA-McStennis USDA-McStennis USDA-McStennis DOE Agenda 2020 So Florida Water Management Dist. USDA-NRI Title Molecular genetics of cellulose biosynthesis in aspen Cryopreservation of tree germplasm Tree biotechnology for enhanced productivity, wood quality, and biosafety Improved wood properties through genetic manipulation of lignin genes Role of extracellular polymers in everglades periphyton dominated biofilms Molecular dissection of alpha-tubulins during microfibril deposition in wood-forming tissue of aspen Genetic engineering of cellulose biosynthesis in hardwood & softwood trees Modulation of cellulose crystallinity in transgenic trees Structure & functions of meniscal horn attachments Cellulose biosynthesis in aspen trees Project M – contact mechanics in the human knee joint A genomics investigation of partitioning into and among flavonoid-derived condensed tannins for carbon sequestration in Populus Genetic modification of gibberellic acid signaling to promote carbon sequestration in tree roots and stems Mechanical stretch & vein graft intimal hyperplasia Genome-enabled modification of poplar root development for increased carbon sequestration A functional genomics approach to investigate regulation of phenolic glycoside metabolism in Populus Research Experiences for Undergraduates Field evaluation of semi-dwarfism transgenes for biosafety of transgenic plants Black bear bone mechanics Funding $14,000 $14,000 $14,000 $1,871,029 $27,600 $229,575 Duration 10/05 - 09/07 10/05 - 09/07 10/05 - 09/07 11/02 - 10/07 10/06 - 10/07 11/02 - 11/07 USDA-NRI ArborGen NIH NSF-CAREER Mayo Clinic DOE $324,332 $18,750 $244,551 $583,883 $63,190 $1,386,389 12/04 - 11/07 01/06 - 12/07 02/05 - 01/08 03/03 - 02/08 11/06 - 06/08 08/05 - 08/08 DOE $289,444 08/05 - 08/08 Goldman J Busov V NIH DOE $271,672 $412,600 09/05 - 09/08 11/05 - 11/08 Tsai CJ, Harding SA, Zhang S Tsai CJ Busov V Donahue S NSF $2,114,091 11/04 - 12/08 NSF-REU Oregon State/ USDA-CSREES NIH $24,596 $167,466 $238,486 05/06 - 12/08 02/05 - 02/09 03/04 - 04/09 - 12 - FY 06 Budget Report The Biotechnology Research Center saw a more than doubled increase in overhead dollars generated through external research grants in FY 06 as compared to FY 05, totaling over $523,000. The overhead return is distributed among the Center (20.7%), PIs (20%, including the standard 6% incentive return), and the PI’s home Department (12%) and College (7.3%). Because of the increased revenue, the BRC launched a 2-year $90K initiative to facilitate infrastructure improvement in 2006. Table. Distribution of BRC Overhead Return by Unit in FY 06: Unit Biology Biomed Mathematics ME-EM SFRES Grand total Overhead generated $6,709 $59,463 $74,349 $35,792 $347,101 $520,387 19.3% Returned to dept/college/school $1,295 $10,892 $14,349 $6,908 $66,991 $100,436 20.7% Returned to BRC $1,389 $11,682 $15,390 $7,409 $71,850 $107,720 20% Returned to PIs $1,342 $11,287 $14,870 $7,158 $69,420 $104,077 The BRC Funds: Total expenses from BRC funds in FY 06 were $82,630. Major categories included BRC seed grants (36.3%), staff salary (35.3%), equipment service/repair/supplies (16.5%), office support (3.8%) and awards and speakers (8.1%). The PI Funds: Total expenses from the PI funds in FY 06 were $80,017. The PI Funds are provided primarily to strengthen individual research programs. Major expenses in FY 06 were research (38.3%, including scientific supplies, chemicals, equipment repair, etc); personnel (32.7%, including research staff, graduate student stipend/tuition/fees, and undergraduate students); and scholarly activities (29%, including office/travel/meeting expenses and journal subscriptions, etc.). Research Expenditures: The Office of the Vice President for Research reported that BRC FY 06 research expenditures totaled $2,382,084, and ranked No. 4 among research centers and institutes on campus, after the Ecosystem Science Center, the Keweenaw Research Center and the Michigan Tech Transportation Institute. BRC Seed Grants for Infrastructure Improvement: A 2-year $90K initiative, BRC Seed Grants for Infrastructure Improvement, was launched in 2006. The funds require a 100% match from Colleges/School, Departments and/or PIs, and are intended to improve research infrastructure that is key for BRC members to successfully develop, complete or renew externally-funded and overhead-generating research grants. Four seed grants have been awarded in 2006, with a BRC commitment of ~$47K toward $153K in equipment and software acquisitions. $10K for a planar chromatography scanner (SFRES, Tsai/Harding), valued at $26,250. $20K for an ApoTome microscope (COE, Goldman/Gilbert/Haut Donahue), valued at $93,327. $10K for a computer cluster with 16 nodes (CSA, Zhang/Chen), valued at ~$20,000 (in process). $7K for a GeneSpring network license (SFRES, Busov/Joshi/Harding/Tsai), valued at $14,188 (in process). - 13 - Peer-reviewed Publications Abdullahi A.S., Underwood G.J.C., Gretz M.R. 2006 Extracellular matrix assembly in diatoms (Bacillariophyceae). V. Environmental effects on polysaccharide synthesis in the model diatom Phaeodactylum tricornutum. Journal of Phycology 42:363-378. Apoya M.D., Yin L., Underwood G.J.C., Gretz M.R. 2006 Movement modalities and responses to environmental changes of the mudflat diatom Cylindrotheca closterium. Journal of Phycology 42:379390. Baker K.C., Anderson M.A., Oehlke S.A., Aastachkina A.I., Haikio D.C., Drelich J., Donahue S.W. 2006 Growth, characterization and biocompatibility of bone-like calcium phosphate layers biomimetically deposited on metallic substrata. Materials Science and Engineering: C, 26: 1351-1360. Bhandari S., Fujino T., Thammanagowda S., Zhang D., Xu F., Joshi C.P. 2006 Coordinate expression of tension stress-responsive and secondary cell wall-associated KORRIGAN endoglucanase and three cellulose synthase genes in aspen trees. Planta 224: 828-837. Brodeur-Campbell S.E., Vucetich J.A., Richter D.L., Waite T.A., Rosemier J.N., Tsai C.-J. 2006 Insect herbivory on low-lignin transgenic aspen. Environmental Entomology 35: 1696-1701. Busov V.B., Meilan R., Pearce D.W., Ma C., Rood S.B., Strauss S.H. 2006 Transgenic modification of gai or rgl1 causes dwarfing and alters gibberellins, root growth, and metabolite profiles in Populus. Planta 224: 288-299. Chandrasekaran R., Thompson M. 2006 Expression, purification and characterization of individual bromodomains from human Polybromo-1. Protein Expr. Purif, I50: 111-117. Chen J., Ramasubramaniam R., Xue C., Liu H. 2006 A versatile molecular engineering approach to simultaneously enhanced multifunctional carbon nanotube polymer composites. Advanced Functional Materials 16: 114-119. Chen J., Xue C., Ramasubramaniam R., Liu H. 2006 A new method for the preparation of stable carbon nanotube organogels. Carbon 44: 2142-2146. Chen. H.S., Sha Q., Zhang S. 2005 A sequential test in family based analysis with parental haplotypes. Genetic Epidemiology 29: 240. Domozych, D.S, Elliott, L., Kiemle, S.N., Gretz, M.R. (in press) Pleurotaenium trabecula, a desmid of wetland biofilms: The extracellular matrix and adhesion mechanisms. Journal of Phycology. Domozych, D.S., Serfis, A., Kiemle, S., Gretz, M.R. (in press) The structure and biochemistry of charophycean cell walls. I. Pectins of Penium margaritaceum. Protoplasma. Donahue S.W., Galley S.A., Vaughan M.R., Patterson-Buckendahl P., Demers L.M., Vance J.L., McGee M.E. 2006 Parathyroid hormone may maintain bone formation in hibernating black bears (Ursus americanus) to prevent disuse osteoporosis. Journal of Experimental Biology 209:1630-1638. Donahue S.W., McGee M.E., Harvey K.B., Vaughan M.R., Robbins C.T. 2006 Hibernating bears as a model for preventing disuse osteoporosis. Journal of Biomechanics 39:1480-1488. Donahue S.W., Galley S.A. 2006 Microdamage in bone: implications for fracture, repair, remodeling, and adaptation. Critical Reviews in Biomedical Engineering 34: 215-271. Eifler R.L., Dehlin J.M., Blough E.R., Haut Donahue T.L. 2006 Glycosaminoglycan production in rabbit meniscal cells due to fluid flow. Journal of Orthopaedic Research 24: 375-384. Filichkin S.A., Meilan R., Busov V.B., Ma C., Brunner A.M., Strauss S.H. 2006 Alcohol-inducible gene expression in transgenic Populus. Plant Cell Reports 25: 660-667. Filichkin S.A., Wu Q., Busov V.B., Meilan R., Lanz-Garcia C., Groover A., Goldfarb B., Ma C., Brunner A., Strauss S.H. 2006 Enhancer trapping in woody plants: isolation of a putative AT-hook motif transcription factor ET304 and characterization of the expression patterns conferred by its promoter in transgenic Populus and Arabidopsis. Plant Science 171: 206-216. Galley S.A., Michalek D.J., Donahue S.W. 2006 A fatigue microcrack alters fluid velocities in a computational model of interstitial fluid flow in cortical bone. Journal of Biomechanics 39: 20262033. Garchow B.G., Jog S.P., Mehta B.D., Monosso J.M. (posthumous), Murthy P.P.N. 2006 Alkaline phytase from Lilium longiflorum: purification and structural characterization. Protein Express. Purification 46: 221-232. - 14 - Gilbert R.J., McKeon R.J., Darr A., Calabro A., Hascall V.C., Bellamkonda R.V. 2005 CS-4,6 is differentially upregulated in glial scar and is a potent inhibitor of neurite extension. Mol. Cell. Neurosci. 29: 545-558. Godin L.M., Donahue H.J., Jacobs C.R., Donahue S.W. (in press) Calcium fingerprints in bone cell mechanotransduction. Biomechanics and Modeling in Mechanobiology. Gupta T., Haut Donahue T.L., 2006 Role of cell location and morphology in the mechanical environment around meniscal cells. Acta Biomaterialia 2: 483-492. 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