Ultra IBD HPLC Columns

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					                                                    Ultra IBD
                                                    HPLC Columns
                                                    Excellent Peak Shape for Acids and Bases
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                                                    Restek's Ultra IBD (intrinsically based deactivated) phase exhibits
                                                    excellent peak shape and selectivity for acids, bases, zwitterions, and
                                                    polar compounds. In contrast to conventional C 18 columns, the Ultra
                                                    IBD phase contains a polar functional group within the hydrocarbon
                                                    bonded chain. This allows the column to retain solutes not only by
                                                    hydrophobic interactions, but also by polar attraction forces between
                                                    the stationary phase and the analyte.

                                                    Features & Benefits

     Restek's Ultra IBD colum
                                                        Separates acids, bases,
      The Ultra IBD can operate under
                                                        zwitterion: and neutral
      both reverse and normal p h a s e
      conditions and retains compound
      across a wide range of mobile                                                                                      ~e                      More flexibility::with method
      phase compositions. Request a                                                                                      pH.
      copy: of Restek's Applications                                                                                                     ¢
      Note cat. #59512. The Ultra IBD                   Excellent : batch to batch
      Column Allows HPLC Separatioi
      of Polar and Non-Polar Analyte
     from the Same                                                                                                                                             ~ed LC/MS sensitivity
     detai!ed information

          Visit our website
     at www.restekcorp.com and see our                         Figurela
           growing compilation of                              Restek's Ultra IBD column exhibits excellent peak shape for acidic
      HPLC application chromatograms:                          and basic compounds at a neutral pH.
      LC_0129 Adenine, Adenosine, ATP,
              ADP, and AMP on Ultra IBD                      Peak List:                 Conc.(pg/mL)                                                                Column:                       Ultra IBD
                                                             1.       uracil                     5                                                                  Catalog #:                    9175565
I LC 0130 Cystosine, Cytidine, CTP,                          2.       benzoicacid               50                                                                  Dimensions:                   150 x 4.6mm
          CDP, and CMP on Ultra IBD                          3.       maleate                    a                                                                  Particle Size:                5um
      LC_B131 Guanine, Guanosine, GTP,                       4.       trimipramine              E                                                                   Pore Size:                    100A
              GDP, and GMP on Ultra IBD                      5.       nortriptyline             E
::                                                           6.       amitriptyline             ~                                                                   Conditions:
i: LC_0132 Thymine, Thymidine, TTP,                                                                                                                                 Mobile Phase:                 20raM K~HyPO    4
           TDP, and TMP on Ultra IBD                         Sample:                                                                                                                              pH 7:acetonitrile
                                                             Inj.:                          10                                                                                                    (65:35,v/v)
!. LC 0130 Uracil, Uridine, UTP, UDP,                                                                                                                               Flow:                         1mL/min.
;    -     and UMP on Ultra IBD                                                                                                                                     Temp.:                        30°C
                                                                                                                                                                    Det.:                         UV @ 254nm




                                                                                                                             i

                                                                      LC_0056                            0                   5                        10                15               20                 25 min.


        CHROMalytic TECHnology Pty Ltd                                          . . . distributors in Australia for . . . RESTEK
        Website : http://www.chromtech.net.au e-mail : info@chromtech.net.au Tel : +61 3 9762 2034 Fax : +61 3 9761 1169
             Iltra IBC                     Peak shape of basic compounds on bonded phase silica can be
                                           improved by lowering the pH to protonate active silanols. However,
                                           doing so can cause problems with some samples that are labile at acidic
                                           pH conditions. The Ultra IBD column performs well even under neutral
                                           pH mobile phase conditions. Figures la and lb show the separation of
                                           acidic and basic compounds under neutral and acidic mobile phase
                                           conditions. The two elution order reversals between Figures la and lb
                                           demonstrate how selection of pH can be used to alter selectivity.




Figure lb
Restek's Ultra IBD column exhibits excellent peak shape for acidic and basic compounds at an acidic range of pH.

Peak List: Conc.(pg/mL)                                                                                     Ultra IBD
1. uracil         5                                                                              #:         9175565
2. unknown                                                                                       ons;       150 x 4.6mm
3. maleate        5                                                                              Size:      5pm
4. benzoicacid   50                                                                              ;e:        100A
5. nortriptyline 50                                                                              )ns:
6. amitriptyline 50                                                                              )hase:     20mM KH2PO      4
7. trimipramine  50                                                                                         pH 3:acetonitrile
                                                                                                            (70:30, v/v)
Sample:                                                                                                     1mL/min.
Inj.:      10ul                                                                                             3000
Solvent:   mobile phase                                                                                     UV @ 254nm




                          I            I            ]          I             I              I

                          0            2            4          6            8               10 min.

                                                                                                                            LC_0057




Figure 2
Ultra IBD shows great selectivity for Carbamates.
Peak List:
 1. aldicarb sulfoxide
 2. aldicarb sulfone
 3. oxamyl
 4. methomyl
 5. 3-hydroxycarbofuran                                                            Column:              Ultra IBD
 6. aidicarb                                                                       Catalog #:           9175565
 7. propoxur                                                                       Dimensions:          150 x 4.6mm
 8. carbofuran                                                                     Particle Size:       5pro
 9. carbaryl                                                                       Pore Size:            100A
10. methiocarb
                                                                                   Conditions:
Sample:                                                                            Mobile Phase:        Gradient program:
Inj.:      5uL                                                                                          (80:20 to 20:80
Solvent:   methanol                                                                                     water:methanol
Conc.:     lmg/mL each                                                                                  in 15 minutes)
           component                                                               Flow:                lmlEmin.
                                                                                   Temp.:               ambient
                                                                                   Det.:                UV @ 220nm




                               I   I
                              0    2                                                                                        LC_0134
Commonly Asked Questions                                                                              Ultra IBD



                                                                                                                  At-a-Glance
• Why is the Ultra IBD different from other HPLC columns?                                                         Product
The Ultra IBD phase incorporates a polar functional group within the                                              Information
                                                                                                                  from Restek
hydrocarbon chain, resulting in a column that exhibits excellent peak
shape for acids, bases, zwitterions, and neutral compounds. The unique
chemistry of this phase allows compounds to be retained by both
hydrophobic and polar interactions.



• What advantage does the Ultra IBD offer f o r LC/MS analyses?
                                                                                                           Restek is your free
Mobile phases with high aqueous content are required to obtain adequate
                                                                                                  technical literature source!
retention of polar analytes with most conventional reverse phase
packings. However, mobile phases with high aqueous content can cause                                              Application Notes:
sensitivity loss in LC/MS analyses. The polar interaction of the Ultra                                   (#59511) Improved HPLC
IBD phase provides excellent retention with higher organic content                                           Analysis of Analgesics
mobile phases, and also greatly reduces or eliminates the need for                           (#59512) The Ultra IBD Column Allows
mobile phase modifiers that are often required to obtain symmetrical                        HPLC Separation of Polar and Non-Polar
peaks and/or sufficient retention when analyzing highly polar com-                                 Analytes from the Same Sample
pounds with other columns. This makes the Ultra IBD much more                             (#59510) HPLC Stationary Phase Selection
compatible with MS detection than traditional straight chain alkyl                                     for the Analysis of Steroids
stationary phases.
                                                                                          (#59118) Allure" PFP Propyl HPLC Column
                                                                                              Provides Improved LC/MS Analyses of
                                                                                                                 Basic Compounds
• What are the particle and pore sizes o f the silica used f o r the                         (#59141) Analyze Nucleotides, Nucleo-
Ultra IBD phase?                                                                                 sides, Purine, and Pyrimidine Bases
                                                                                           Simultaneously with the Ultra IBD Column
The Ultra IBD is offered in both 3 and 5um, spherical Type B silica
with a 100 Angstrom pore size.



• What are the p H and temperature limits f o r the Ultra IBD                                         • Call 814-353-1300 or
phase?                                                                                                   800-356-1688, ext. 5
The Ultra IBD phase can be used from pH 2 to pH 8 and at tempera-                                        • Fax 814-353-1309
tures up to 80°C.                                                                               • Online www.restekcorp.com



• Are guard columns available f o r the Ultra IBD columns?
Restek's unique Trident guard column system is available for the Ultra
IBD column. The integral leak-free design of the Trident system
contains a cap frit that can be replaced easily without disturbing the
packed bed. The guard cartridge, packed with the Ultra IBD phase also
can be replaced easily as needed.




     CHROMalytic TECHnology Pty Ltd                         . . . distributors in Australia for . . . RESTEK
     Website : http://www.chromtech.net.au e-mail : info@chromtech.net.au Tel : +61 3 9762 2034 Fax : +61 3 9761 1169
         Jltra IBI                         • Ultra IBD 3um Columns
                                              Column         1.0mm ID      2.1mm ID           3.2mm ID       4.6mm ID
                       At-a-Glance            Length           cat.#         cat.#                 cat.#       cat.#
                       Product
                       Information
                       from Restek



                                           • Ultra IBD 3um Columns with Trident Inlet         TM



                                              Column           2.1mm ID                3.2mm ID            4.6mm ID
                                              Length              cat.#                   cat.#               cat.#




                                           • Ultra IBD 5um Columns
                                              Column         1.0mm ID      2.1mm ID           3.2mm ID       4.6mm ID
                                              Length           cat.#          cat.#              cat.#          cat.#




            Bellefonte, PA 16823
             fax: 81 4-353-1309
            RestekFrance
            (33) 01-60-78-32-1(i
         fax: (33)01 6078 7090             • Ultra IBD 5um Columns with Trident Inlet             TM



                                              Colunm           2.1mm ID                3.2mm ID            4.6mm ID
                                              Length              cat.#                   cat.#               cat.#
              (49) 0 6 1 7 2 - 2 Z 9 7 0
        fax: (49) 06172-2797 7~
           Restekgmbh@aoLcom
           ThamesRestek U.K.
              (44) 01753-624111i
         fax: (44)01753,624666
         SALESIHRES@aoLcOm

                                           • Ultra IBD Guard Cartridges
www,restekcorp
                                                imensions                      cat.#                           qt~




   CHROMalytic TECHnology Pty Ltd                           . . . distributors in Australia for . . . RESTEK
   Website : http://www.chromtech.net.au e-mail : info@chromtech.net.au Tel : +61 3 9762 2034 Fax : +61 3 9761 1169

     .it. Cat. # 596141