FRUIT CROPS CERTIFICATION STANDARDS by qov12652

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									STANDARD GUIDELINES
    FOR PLANTING
    MATERIAL OF
   HORTICULTURAL
1.   FRUITS
Mango (Mangirfera indica L.)

1.   Application and Amplification of General Clone Certification
Standards

a.          The General Clone Certification Standards are basic and together
            with the following specific standards constitute the standards to
            certification of mango clones.
b.          The General Standards are amplified as follows to apply specifically
            to mango.
            All certified clones shall be produced by asexual methods like
            veneer grafting or soft wood grafting or epicotyls grafting or
            inarching

II.         Land Requirements

       a.      Land to be used for clonal propagation of mango shall be free
               from volunteer plants
       b.      Soils with high chloride content should be avoided

III.        Field Inspection

a.          Root stock
                  A minimum of one inspection shall be made before grafting
            when the rootstock has attained graftable stage

b.          Mother Plant/Scion

                  Mother Plant should be healthy, true to type and free from
            diseases and pests. The trees should be certified for the desirable
            characters by the Certifying agency and a certificate to this effect
            shall be given to the nurseries. A minimum of one inspection shall
            be made at the time of fruit maturity for health and fruit quality of
            the mother tree.

c.          Grafted Clones

                  A minimum of two inspections shall be made before the sale
            of the clones after attaining the specified size to verify relevant
            factors.

IV.         Field Standards
A.          General Requirements
1.    Isolation: The clone propagation plots of mango shall be isolated
      from the contaminants as shown in the following table.

Contaminants                   Minimum                distance Certified
                               (meters)
                               Foundation
Field of other varieties       3                               3
Fields of the same variety not 3                               3
conforming to varietal purity
requirements for certification

2.    Spacing: The spacing between plants in clone propagation plots
      should be 20-30 cm and between rows 30-40 cm.
1.    Rotation: Nursery should be rotated to other plots after raising
      mango three times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in Absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from malformation, anthracnose and
dieback.

b.    Certified Class
Factor                                 Maximum permitted (%)*
                                       Certified
Off type                               0.10
Plants infected with malformation      0.20
or anthracnose
Plants infected with dieback           None
* Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    the diameter of the stock should range from 1.0-1.25 cm
2.    The height of the grafting should range from 15-25 cm
3.    The diameter of the grafts at 10 cm above the graft union should
      range from 1.25-1.5 cm and height of the graft 60-100 cm.
4.    Height of the plant should be 25-40 cm in case of
5.    The grafted clones should be free from suckers.
6.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                              Standard for each class
Factor
                              Foundation            Certified
Pure living clones (minimum) 99.5%                  98.0%
                              (by number)           (by number)
Other living plants including 0.5%                  2.0%
rootstocks (maximum)          (by number)           (by number)
Plants infected with dieback  None                  None
Sweet Orange (Citrus chinensis Osbeck)

I.     Application and Amplification of General Clone Certification
       Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of sweet orange clones.
b.     The General Standards are amplified as follows to apply specifically
       to sweet orange.
       All certified clones shall be produced by asexual methods like
       shield budding or patch budding.

II.    Land Requirements
a.     Land to be used for clonal propagation of sweet orange shall be free
       from volunteer plants.

III.   Field Inspection
a.     Rootstock
       A minimum of one inspection shall be made before budding when
       the rootstock has attained graftable stage.
b.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
c.     Budded Clones
       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.
IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of mango shall be isolated
       from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification
2.    Spacing: The spacing between plants in clone propagation plots
      should be 25 cm and between rows 40 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      mango two times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in Absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from malformation, anthracnose and
      dieback.

b.    Certified Class
       Factor                          Maximum permitted (%)*
                                       Certified
       Off type                        0.10
       Plants infected with dieback None
      * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the stock should range from 0.6-1.00 cm
2.    The height of the grafting should range from 15-20 cm
3.    The diameter of the grafts at 10 cm above the graft union should
      range from 1.0-1.25 cm and height of the Budd 30-50cm.
4.    The Budded clones should be free from suckers.
5.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                              Standard for each class
Factor
                              Foundation            Certified
Pure living clones (minimum) 99.5%                  98.0%
                              (by number)           (by number)
Other living plants including 0.5%                  2.0%
rootstocks (maximum)          (by number)           (by number)
Plants infected with dieback  None                  None
Mandarin (Citrus reticulate Blanco)

I.     Application and Amplification of General Clone Certification
       Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of mandarin clones.
b.     The General Standards are amplified as follows to apply specifically
       to mandarin.

       All certified clones shall be produced by asexual methods like
       shield budding or patch budding.

II.    Land Requirements
a.     Land to be used for clonal propagation of mandarin shall be free
       from volunteer plants.

III.   Field Inspection
a.     Rootstock
       A minimum of one inspection shall be made before budding when
       the rootstock has attained buddable stage.
b.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of two inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
c.     Budded Clones
       A minimum of two inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.
IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of mango shall be isolated
       from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification
2.    Spacing: The spacing between plants in clone propagation plots
      should be 25 cm and between rows 40 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      mango two times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from viruses.

b.    Certified Class
       Factor                           Maximum permitted (%)*
                                        Certified
         Off type                       0.10
         Plants infected with dieback None
           * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the stock should range from 0.6-1.00 cm
2.    The height of the grafting should range from 15-20 cm
3.    The diameter of the grafts at 10 cm above the graft union should
      range from 1.0-1.25 cm and height of the Budd 30-50cm.
4.    The Budded clones should be free from suckers.
5.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                              Standard for each class
Factor
                              Foundation            Certified
Pure living clones (minimum) 99.5%                  98.0%
                              (by number)           (by number)
Other living plants including 0.5%                  2.0%
rootstocks (maximum)          (by number)           (by number)
Plants infected with dieback  None                  None
Acid lime (Citrus aurantium Swingle)

I.   Application and Amplification of General Clone Certification
Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of sweet orange clones.
b.     The General Standards are amplified as follows to apply specifically
       to sweet orange.
       All certified clones shall be produced by layering or nucellar
       seedlings.

II.    Land Requirements
       Land to be used for clonal propagation of sweet orange shall be free
       from volunteer plants.

III.   Field Inspection
a.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
c.     Clones (Layers/Nucellar Seedlings)
       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of Acid Lime shall be
       isolated from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification

2.     Spacing: The spacing between plants in clone propagation plots
       should be 25 cm and between rows 30 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      acid lime two times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from viruses, canker and greening.

b.    Certified Class
       Factor                           Maximum permitted (%)*
                                        Certified
         Off type                       0.10
         Plants infected with dieback None
           * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the stock should range from 0.6-1.00 cm and
      height of the clone 20-40 cm
2.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                             Standard for each class
Factor
                             Foundation            Certified
Pure living clones (minimum) 99.5%                 98.0%
                             (by number)           (by number)
Plants with virus, canker or None                  None
greening
Lemon (Citrus limon Burn.)

I.   Application and Amplification of General Clone Certification
Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of lemon clones.
b.     The General Standards are amplified as follows to apply specifically
       to lemon.

       All certified clones shall be produced by asexual methods like air
       layering or cuttings.

II.    Land Requirements
a.     Land to be used for clonal propagation of lemon shall be free from
       volunteer plants.

III.   Field Inspection
a.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
b.     Budded Clones
       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.
IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of lemon shall be isolated
       from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification

2.     Spacing: The spacing between plants in clone propagation plots
       should be 20 cm and between rows 30 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      lemon two times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from viruses and canker.

b.    Certified Class
       Factor                            Maximum permitted (%)*
                                         Certified
         Off type                        0.10
         Plants infected with dieback None
             * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the clone at 10 cm above the ground level should
      range from 1.0-1.25 cm and height of the Budd 25-40cm.
2.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                                  Standard for each class
Factor
                                  Foundation            Certified
Pure living clones (minimum)      99.5%                 98.0%
                                  (by number)           (by number)
Plants infected with dieback      None                  None
Guava (Psidium guajava L.)

I.     Application and Amplification of General Clone Certification
       Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of guava clones.
b.     The General Standards are amplified as follows to apply specifically
       to guava.

       All certified clones shall be produced by asexual methods like
       patch budding or ring budding or stooling or inarching or air
       layering.

II.    Land Requirements
a.     Land to be used for clonal propagation of guava shall be free from
       volunteer plants.

III.   Field Inspection
a.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
b.     Clones (Budded/Grafted/Stool layers/Air layers)
       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.
IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of guava shall be isolated
       from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification

2.     Spacing: The spacing between plants in clone propagation plots
       should be 20 cm and between rows 30 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      guava three times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in absolute terms. Off types should be discarded
      under the supervision of Certification Agency.

b.    Certified Class
       Factor                         Maximum permitted (%)*
                                      Certified
      Off type                        0.10
      Plants infected with dieback None
          * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the stock should range from 0.75-1.00 cm
2.    The height of the grafting should range from 10-15 cm
3.    The diameter of the grafts at 10 cm above the graft union and self
      rooted plants at 10 cm above ground level should range from 1-
      1.25 cm and height 50-75 cm.
4.    The grafted clone should be free from suckers.
5.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                              Standard for each class
Factor
                              Foundation            Certified
Pure       living      clones 99.5%                 98.0%
(minimum)                     (by number)           (by number)
                              0.5%                  2.0%
Other living plants including (by number)           (by number)
rootstocks (maximum)
Apple (Malus domestica Brokh.)

I.     Application and Amplification of General Clone Certification
       Standards

a.     The General Certification Standards are basic and together with
       the following specific standard constitute the standards for
       certification of apple clones.
b.     The General Standards are amplified as follows to apply specifically
       to apple.

       All certified clones shall be produced by asexual methods like
       tongue grafting or chip budding.

II.    Land Requirements
a.     Land to be used for clonal propagation of apple shall be free from
       volunteer plants.

III.   Field Inspection
a.     Rootstock
       A minimum of one inspection shall be made before
       grafting/budding when the rootstock has attained graftable stage.

b.     Mother Plant/Scion
       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.
c.     Grafted / Budded Clones
       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.
IV.    Field Standards
A.     General Requirements
1.     Isolation: The clone propagation plots of apple shall be isolated
       from the contaminants as shown in the following table.

       Contaminants                       Minimum              distance Certified
                                          (meters)
                                          Foundation
       Field of other varieties               3                           3
       Fields of the same variety not 3                                   3
       conforming      to     varietal purity
       requirements for certification
2.    Spacing: The spacing between plants in clone propagation plots
      should be 20 cm and between rows 30 cm.
3.    Rotation: Nursery should be rotated to other plots after raising
      apple three times in the same plot.

B.    Specific Requirements
a.    Foundation Clones
1.    Foundation clones being a group of common ancestry shall be
      genetically pure in absolute terms. Off types should be discarded
      under the supervision of Certification Agency.
2.    The plants should be free from woolly aphis, collar rot, rubbery
      wood and sanjose scale.

b.    Certified Class
       Factor                         Maximum permitted (%)*
                                      Certified
      Off type                        0.10
      Plants infected with woolly None
      aphis, collar rot, rubbery
      wood or sanjose scale
          * Standards for off types shall be met at final inspection.

V.    Clone Specifications
      The specifications in respect of size of clones for foundation and
      certified classes shall be as follows:
1.    The diameter of the stock should range from 0.6-0.75 cm
2.    The height of the grafting should range from 15-20 cm
3.    The diameter of the grafts at 10 cm above the graft union should
      range from 0.75-1.0 cm and height 75 - 120cm.
4.    The grafted clone should be free from suckers.
5.    In the clone lot, clones not conforming to specified size shall not
      exceed 5.0% (by number)
VI.   Clone Standards

                              Standard for each class
Factor
                              Foundation            Certified
Pure       living      clones 99.5%                 98.0%
(minimum)                     (by number)           (by number)
                              0.5%                  2.0%
Other living plants including (by number)           (by number)
rootstocks (maximum)          None                  None
Plants infected with wooly
aphis, collar rot, rubbery
wood or sanjose scale
Banana (Musa paridasiaca L.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of banana clones.

II.    Land requirements

       Land to be used for clonal propagation of banana shall be free from
       volunteer plants and diseases.

III.   Field Inspection
       Mother plant:

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The plants should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.

       Clones (Suckers)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General Requirements

       1.    Isolation: The clone propagation plots of banana shall be
             isolated from the contaminants with 3-meter distance.

       2.    Spacing: The spacing between plants in clone propagation
             plots should be 1.5 m and between rows 1.5 m.

       3.    Rotation: Nursery should be rotated to other plots after
             raising banana three times in the same plot.

B.     Specific requirement :
       Clones
     1.    Foundation clones being a group of common ancestry shall
           be genetically pure in absolute terms. Off types should be
           discarded under the supervision of Certification Agency.

     2.    The plants should be free from bunchy top, nematode and
           Panama disease.

V.   Clone Specifications

     The specifications in respect of size of clones shall be as follows:

     1.    The diameter of the sucker should range from 7.0 - 10.0 cm.

     2.    In the clone lot, clones not conforming to specified size shall
           not exceed 5.0% (by number).
Litchi (Litchi chinensis Sonn.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of litchi clones.

       The General Standards are amplified as follows to apply specifically
       to litchi.

       All certified clones shall be produced by asexual methods viz., air
       layering.

II.    Land requirements

       Land to be used for clonal propagation of litchi shall be free from
       volunteer plants.

III.   Field Inspection

       Mother plant:

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.

       Clones (Air Layers)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.     General requirements

       1. Isolation: The clone propagation plots of litchi shall be isolated
          from the contaminants with 3-meter distance.
      2. Spacing: The spacing between plants in clone propagation plots
         should be 30 cm and between rows 50 cm.

      3. Rotation: Nursery should be rotated to other plots after raising
         litchi three times in the same plot.

B.    Specific requirement :

      Nursery:

      1. Clones being a group of common ancestry shall be genetically
         pure in absolute terms. Off types should be discarded.

      2. The Off-types should not exceed to 0.1% by number.
VI.   Clone Specifications

      1. The diameter of the plant should range from 1.0 - 1.5 cm and
         height of the plant 10-75 cm.

      2. In the clone lot, clones not conforming to specified size shall not
         exceed 5.0% (by number).
Grape (Vitis vinifera L.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of grape clones.

       The General Standards are amplified as follows to apply specifically
       to grape.

       All certified clones shall be produced by asexual methods like chip
       budding or cutting.
II.    Land requirements

       Land to be used for clonal propagation of grape shall be free from
       volunteer plants.

III.   Field Inspection

       Rootstock

       A minimum of one inspection shall be made before budding when
       the rootstock has attained buddable stage.

       Mother plant:/Scion

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother Block.

       Clones (Budded/rooted cuttings)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General requirements
      1. Isolation: The clone propagation plots of grape shall be isolated
         from fields of the same variety not conforming to purity and
         field of other varieties by 3-meter (Minimum).

      2. Spacing: The spacing between plants in clone propagation plots
         should be 20 cm and between rows 30 cm.

      3. Rotation: Nursery should be rotated to other plots after raising
         grape three times in the same plot.

B.    Specific Requirement :

      1. Clones being a group of common ancestry shall be genetically
         pure in absolute terms. Off types should be discarded.
      2. The plants should be free from nematode and anthracnose.

      3. The Off-types should not exceed from 0.1% by number
         (maximum).

VI.   Clone Specifications

      1. The diameter of the stock should range from 1.0 - 1.25 cm.

      2. The height of the budding should range from 8-10 cm.

      3. The diameter of the grafts/rooted cuttings should range from
         1.0-1.25 cm and height 25-40 cm.

      4. The grafted clone should be free from suckers.
Pineapple (Ananas comosus Merr.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Pineapple clones.

       The General Standards are amplified as follows to apply specifically
       to Pineapple.

       All certified clones shall be produced by asexual methods like
       suckers or slips.
II.    Land requirements

       Land to be used for clonal propagation of Pineapple shall be free
       from volunteer plants.

III.   Field Inspection

       Mother plant:

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The plants should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother plants.

       Clones (Suckers/Slips)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General requirements

       1. Isolation: The clone propagation plots of Pineapple shall be
          isolated from fields of the same variety not conforming to
          varietal purity and field of other varieties by 3-meter
          (Minimum).
     2. Spacing: The spacing between plants in clone propagation plots
        should be 30 cm and between rows 60 cm.

     3. Rotation: Nursery should be rotated to other plots after raising
        Pineapple three times in the same plot.

B.   Specific Requirement :

     1. Clones being a group of common ancestry shall be genetically
        pure in absolute terms. Off types should be discarded.

     2. The plants should be free from mealy bug.

V.   Clone Specifications
     1. The weight of sucker should be 400-500g and slip 350-450g..

     2. In the clone lot, clones not conforming to specified size shall not
        exceed 5.0% (by number).
Sapota (Achras sapota L.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Sapota clones.

       The General Standards are amplified as follows to apply specifically
       to Sapota.

       All certified clones shall be produced by asexual methods like
       inarching or air layering.
II.    Land requirements

       Land to be used for clonal propagation of Sapota shall be free from
       volunteer plants.

III.   Field Inspection

       Rootstock:

       A minimum of one inspection shall be made before grafting when
       the rootstock has attained graftable stage.

       Mother plant/Scion:

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The plants should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.

       Clones (Grafted/Air Layers)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General requirements
     1. Isolation: The clone propagation plots of Sapota shall be
        isolated from fields of the same variety not conforming to
        varietal purity and field of other varieties by 3-meter
        (Minimum).

     2. Rotation: Nursery should be rotated to other plots after raising
        Sapota three times in the same plot.

B.   Specific Requirement :

     1. Clones being a group of common ancestry shall be genetically
        pure in absolute terms. Off types should be discarded.

     2. The Off-types should not be more than 0.10% (maximum).
V.   Clone Specifications

     1. The diameter of the stock should range from 1 - 1.25 cm.

     2. The weight of the grafting should range 15-25 cm.

     3. The diameter of the grafts at 10 cm above the graft union
        should range from 1 - 1.25 cm and height 50 to 75 cm
        (inarched) and 30-60 cm (air layered).

     4. The grafted clone should be free from suckers.

     5. In the clone lot, clones not conforming to specified size shall not
        exceed 5.0% (by number).
Jackfruit (Artocarpus heterophyllus Lam.)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Jackfruit clones.

       The General Standards are amplified as follows to apply specifically
       to Jackfruit.

       All certified clones shall be produced by asexual methods like
       patch budding or ring budding or veneer budding.
II.    Land requirements

       Land to be used for clonal propagation of Jackfruit shall be free
       from volunteer plants.

III.   Field Inspection

       Rootstock:

       A minimum of one inspection shall be made before budding when
       the rootstock has attained buddable stage.

       Mother plant/Scion:

       Mother Plant should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother tree.

       Budded Clones

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General requirements
     Isolation: The clone propagation plots of Jackfruit shall be isolated
     from fields of the same variety not conforming to varietal purity
     and field of other varieties by 3-meter (Minimum).

B.   Specific Requirement :

     1. Clones being a group of common ancestry shall be genetically
        pure in absolute terms. Off types should be discarded.

     2. Off-types should not be more than 0.10% (maximum).

V.   Clone Specifications

     1. The diameter of the stock should range from 1 - 1.25 cm.
     2. The height of the grafting should range 15-20 cm.

     3. The diameter of the grafts at 10 cm above the graft union
        should range from 1.25 - 1.50 cm and height of the graft 30 to
        50 cm.

     4. The grafted clone should be free from suckers.

     5. In the clone lot, clones not conforming to specified size shall not
        exceed 5.0% (by number).
Passion fruit (Passiflora edulis Sims)

I.     Application and Amplification of General Clone Certification
       Standards.

       The General Clone Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Passion fruit clones.

       The General Standards are amplified as follows to apply specifically
       to Passion fruit.

       All certified clones shall be produced by asexual means viz.,
       cutting.
II.    Land requirements

       Land to be used for clonal propagation of Passion fruit shall be free
       from volunteer plants.

III.   Field Inspection

       Mother plant(s)

       Mother Plant(s) should be healthy, true to type and free from
       diseases and pests. The trees should be certified for the desirable
       characters by the Certifying Agency and a certificate to this effect
       shall be given to the nurseries. A minimum of one inspection shall
       be made at the time of fruit maturity for health and fruit quality of
       the mother plant(s).

       Clones (Rooted cuttings)

       A minimum of one inspection shall be made before the sale of the
       clones after attaining the specified size to verify relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation: The clone propagation plots of Passion fruit shall
             be isolated from fields of the same variety not conforming to
             varietal purity and field of other varieties by 3-meter
             (Minimum).
     2.      Rotation: Nursery bed should be rotated to other plots after
             raising passion fruit three times in the same plot.

B.   Specific Requirement :

     1. Clones being a group of common ancestry shall be genetically
        pure in absolute terms. Off types should be discarded.

     2. Off-types should not be more than 0.10% (maximum).

V.   Clone Specifications

     1. The diameter of the plants at 10 cm above ground level should
          range from 0.42 - 0.60 cm and height of the plant 15 to 25 cm.
     2. In the clone lot, clones not conforming to specified size shall not
        exceed 5.0% (by number).
                         TISSUE CULTURE BANANA



Tissue Culture Raised Plants : Standard guidelines/parameters

Plant tissue culture or micropropagation is the aseptic culture of cells,
pieces of tissue, or organs. It is the true-to-type propagation of a selected
genotype using in vitro culture techniques. By this technique, it is
possible to regenerate new plants from small pieces of plant tissue
because each cell of a given plant has the same genetic makeup and is
capable of developing along a pre-determined pathway leading to the
formation of an entire plant that is identical to the plant from which it
was derived. It has been ground that plants can reproduce whole plants
from fragments of plant material when given a nutrient media capable of
supporting    growth     and    appropriate    hormone     control.    Using
micropropagation, millions of new plants can be derived from a single
plant. Micropropagation can be used to establish and maintain virus
infected, even though the remainder of the plant may be. Once new
plants are developed from the apical meristem, they can be maintained
and distributed as virus-free plants.

The standard process flow chart for production of quality planting
material is as follows

1.    Establishment and maintenance of 'Elite certified mother nursery'

2.    Selection of mother plants - Testing for virus & quality

3.    Explant

4.    Multiplication through axillary bud/meristem culture

5.    Rooting, primary, secondary hardening and rouging

6.    Periodic identification of somaclonal variations and off-types

7.    Nursery plantation - Testing for virus & quality
8.        Field   transfer   -   Testing   for   quality,   Virus   indexing    and
          Certification

Criteria for production of quality planting material

I.        Evaluation of Tissue Culture Labs

A tissue culture lab is governed by infrastructure facilities, quality
control mechanism and competence of the technical supervision. Each of
these areas have a prominent role in ensuring the production of good
quality planting material.

Score Card for Assessing TC Units.

Sl.No      Description                                                   Marks
.

     1.    Infrastructure and Lab facilities

            Washing room                                                      5

            Media preparation room                                            10

            Inoculation room                                                  10

            Growth room                                                       10

     2.    Quality control

            Maintenance of certified mother block nurseries

                  Selection of mother plants                                   10

                  Periodic virus indexing of mother block nursery              10

                  Explant selection and culture initiation                     5

            Virus indexing of explant                                         5

            Clonal fidelity and number of multiplication                      10

                  Identification of somaclonal variations and off-
               types*                                                 5

         Overall quality of the plants

  3.    Hardening facilities

         Transfer area, Greenhouse/shade area, Nursery              10

  4.    Technical supervision and monitoring                         10

         Monitoring of the production process and the staff
            involved therein

         Technical competence of the production supervisory
            staff

         Operators

                                                         TOTAL      100




The TC I units getting an overall score of less than 65 should not be
considered eligible for distribution of micropropagated planting material
till such time when the facilities are improved as per norms. The lab
facilities including hardening facilities carry the maximum marks of 60
followed by quality control with 30 marks. These two areas are most
crucial for enabling the production and supply of disease free planting
material.

II.    Testing for virus and quality

Tissue culture is an useful approach for generating virus-free planting
material. In order to minimize the risk of inadvertent propagation of virus
infected plants and introduction of somaclonal variability, tissue culture
raised plants need to be thoroughly indexed for freedom from viruses and
checked for quality. Careful indexing based on recent biotechnological
methods such as immunoprobes, nucleic acid probes and polymerase
chain reaction (PCR) would ensure phytosanitary safety during the
movement of planting material. Similarly, molecular testing will ensure
quality control. The PCR based technique known as Randomly Amplified
Polymorphic DNA (RAPD) provides a reliable methodology for identifying
genotypes and studying the genetic variation.

Virus testing

Various immunological and molecular techniques like ELISA, DIBA,
Western blotting, immunofluorescence, nucleic acid hybridisation using
radio-labelled probes have been used successfully for the diagnosis of
virus infection in a variety of plant species, ELISA, immuno-electron
microscopy, dot-blot and PCR are the most sensitive and reliable and can
be very effectively used for the purpose of diagnostic services to tissue
culture industry in the country. Nucleic and hybridization, c-DNA probes
are also available for the Gemini-, poty-, tobamo-, and badnaviruses.
Electrophoresis and direct electron-microscopy are also being commonly
used particularly for detecting viroids and unknown viruses.

Quality control

With the advent of the PCR technique and DNA based molecular
markers, some laboratories in India expertise in these areas and are
using them for assessment of genetic diversity, phylogenetic analysis for
establishing true to type nature of tissue cultured plants. From the
contrasting reports on the use of molecular marker techniques for
analysis of micropropagated plants, it is essential that the marker system
chosen for analysis needs to be evaluated for its suitability for the
application before it is used.

The above guidelines are generic and would vary in terms of the steps
and parameters for assessment for individual species. Some species-
specific parameters are listed.
* There is a difference of opinion among researchers and production labs
on the number of passages that could be regarded as "safe" for shoot
multiplication in banana with respect to clonal uniformity of plants. In
tissue culture it is well known that lesser the number of subcultures, lower
will be the chances of somaclonal variation. However, it must also be
realized that if the number of passages are far too small then the entire
production   process becomes economically unviable. Therefore, efforts
should be made to optimize the shoot multiplication process and extend the
number of passages only till the clonal uniformity of the progenies is
maintained. This could be achieved through a) strict monitoring of shoot
multiplication process ensuring that adventitious shoots are not multiplied
and b) confirming the clonal fidelity of tissue cultured plants using
molecular markers in different passages. However, in banana under no
circumstances shoots should be subcultured for more than 8 passages.
Quality Standards for production through tissue culture

Standards for Laboratory and Greenhouse Facilities

1.      All micropropagation and greenhouse facilities must fulfill the
        standards set by the competent authority.

2.      Laboratory and greenhouse facilities used for production of
        plantlets shall be maintained free of pests or vectors of banana
        pathogens. Failure to keep such pests under control may cause
        rejection of all lots maintained in the facility. All potting or growth
        media shall be sterile. Water sources used in the laboratory or
        greenhouse operation shall be treated or otherwise rendered free of
        all possible pathogens by the applicant.

3.      Hygienic       conditions   shall     be     strictly    observed     during
        micropropagation, potting, planting, irrigating, movement and use
        of equipment and other laboratory and greenhouse practices to
        guard against the spread of diseases or pests in the facilities used
        for banana plant multiplication.



Minimum        Quality      Standards       for    growing      of   plants   inside
greenhouses/polyhouses

The following requirements must be met for production of plantlets :

1.      Elite mother nursery block' and Selection of mother plants

    Mother plant should be healthy, true to type and free from diseases
     and pests especially virus diseases. It should be certified for the
     desirable characters by the certifying agency and a certificate shall be
     given to the nurseries. Minimum two inspections shall be made at the
     time of vegetative phase (6th month) and at the time of flowering and
     fruiting stage.
    Mother plants whose varietal purity is confirmed through molecular
     markers should be used as source plants.

    The male flower bud should be retained to check the presence of virus
     diseases (Male flower bud exhibits symptoms of late infection of
     viruses like BBTV and BBMV).

    Mother plants should be raised under topless insect proof shade net
     with sufficient height.

    Isolation distance - Mother nursery location must be away from other
     banana plantations with an isolation distance of 500m to maintain
     purity and to avoid spread of virus diseases.

    Mother plants should be grown under very good management
     conditions so has to facilitate the true expression.

    Individual plant should given a master code number so that the
     plantlets developed could be traced back to the mother plant.

    Pedigree record and source of each mother plant should be
     maintained and catalogued.

    Varieties must be separated by physical barriers, which will prevent
     varietal mixture.

2.      Virus indexing of mother plant nursery

    Should be done twice during its crop duration,

        1. at 6 months stage

        2. and at fruiting stage

    The indexing should be carried out primarily for four viruses, namely
     Banana Bunchy top Virus, Banana Bract mosaic virus, Banana
     Streak virus and Cucumber mosaic virus.
    The entire clump of plant, suckers along with infected mother
     underground corm should be removed and destroyed.

3.      Explant selection and culture initiation

Choice of explant is vital

    Well maintained mother plants should be selected

    The sucker should be healthy and not less than 60-80 days of age

    Growing meristem should be of 1.0 cubic cm in size

    The explant should not be cut to avoid injury to the growing meristem
     and eventual abnormalities

4.      Number of subculture cycles and rouging

a.      No. of subculture cycle

        The maximum number of subculture cycles should be 6 or less
        and should not be more than 7

b.      Rouging at various stages of proliferation

           Only true to type proliferating buds should be selected for
            further sub culturing

           Differentiated plant in the culture bottle should exhibit true to
            type plant traits and those exhibiting abnormal growth should
            be discarded

5.      Rooting, primary hardening and rouging

        (Ex agar Management and selection of proper container and
        potting mixtures)

           The rooting media should be 100 per cent free from pathogen

           Water used for irrigating the plants should be free from
            pathogens and nematodes
        Strict rouging by trained personnel should be done to remove
         the off types once in 7 days

        Sample plants from each batch should be randomly virus
         indexed (at least 10 plants from each batch/explant)

        While shifting of primary hardened plants, two longitudinal cuts
         of net pots should be given to facilitate further corm growth.

        Indexing of plants at nursery stage - 1% from each batch
         will be indexed.

        True to type quality assessment using molecular markers -
         10% of the pants have to be assessed.

6.   Maintenance of Pedigree Block

7.   Plants from each batch should be maintained for one year for
     cross confirmation.

8.   Ideal Tissue Culture Plant

        Plant should be free from all viral diseases and confirmed
         through virus indexing.

        Plant is confirmed for its true to its type.

        A well hardened plant should be 30 cm in height and should
         have a pseudostem circumference of 5.0-6.0 cm after 60 days of
         hardening.

        The plant should have 5 photosynthetically active leaves and
         inter foliar space must be not less than 5.0 cm

        The plant should have approximately 25-30 active roots at he
         end of secondary hardening stage

        The length of active roots should be more than 15 cm with a
         good number of secondary roots
          The polybag should be of size (20.0 cm in length and 16 cm in
           diameter) with potting media filled to ¾ full of the bag

          The media/potting mixture approximately should weigh about
           750-800 g. on dry weight basis

          Plantlets should be free from any visual symptoms of leaf spot,
           pseudostem rot and physical deformations.

          Plantlets should be free from the presence of root pathogens like
           Erwinia rot symptoms, nematode lesions and root knots.
           Random checking of roots is very essential at the time of
           procurement

          Those exhibiting abnormal growth must be discarded

Certified class

                Factor                            Max. Permitted

Off type                                                 <1%

Plants infected with virus diseases                     None

Plants infected with nematodes                          None

Plants infected with fungal diseases                    None

Banana is highly susceptible to tissue cultured somaclonal variation and
it varies from 4-40% depending on the variety. All Cavendish clones
including, Grand Naine, Robusta, Dwarf Cavendish, Giant Cavendish,
Shrimanti, Williams etc. are highly susceptible. This group is followed by
Plantain group which includes Nendran and its ecotypes. Mysore and
Poovan group are medium susceptible to variations. Keeping a broad
spectrum      of   susceptibility     among    varieties,   the   maximum
acceptable variation <1%
2. VEGETABLES
BRINJAL (Solanum melonegna L.)

I.   Application and Amplification of General Seed Certification
Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Brinjal seed.

II.    Land requirements

       Land to be used for seed production of Brinjal shall be free of
       volunteer plants.

III.   Field Inspection
       A minimum of three inspections shall be made, the first before
       flowering, the second at the flowering and fruiting stage and the
       third at mature fruit stage and prior to harvesting.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said table:

Contaminants                               Minimum distance (meters)

                                           Foundation        Certified

                    1                           2                3

Fields of other variety                        300             150

Fields of the same variety not                 300             150
conforming     to     varietal purity
requirements for Certification
B.    Specific requirements

Factor                                    Minimum              permitted
                                          (%)*distance

                                           Foundation        Certified

                   1                             2               3

Off types                                      0.10             0.20

** Plants infected seed borne diseases         0.10             0.20

* Standards for off types shall be met at and after flowering and for seed
borne disease at final inspection.

** Seed borne disease shall be: Phomopsis blight (Phomopis vexans (Sacc.
& Syd.) Harter.)

V.    Seed standards

Factor                                      Standard for each classes

                                           Foundation        Certified

                   1                             2               3

Pure seed (minimum)                           98.0%            98.0%

Inert matter (maximum)                         2.0%             2.0%

Other crops seeds (maximum)                    None             None

Weed seeds (maximum)                           None             None

Germination (minimum)                          70%              70%

Moisture (maximum)                             8.0%             8.0%

For vapour-proof containers (max)              6.0%             6.0%
CAPSICUM (SWEET PEPPER) (Capsicum annuum L. Var. grossum
Bailey) AND CHILLI (HOT PEPPER)(Capsicum frutescens l.)



I.   Application and Amplification of General Seed Certification
Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Capsicum and Chilli seed.

II.    Land requirements
       Land to be used for seed production of Capsicum and Chilli shall
       be free of volunteer plants.

III.   Field Inspection

       A minimum of three inspections shall be made, the first before
       flowering, the second at the flowering and fruiting stage and the
       third at mature fruit stage and prior to harvesting.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields offered for certification shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said Table:

Contaminants                                Minimum distance (meters)

                                             Foundation      Certified

                    1                               2            3

Fields of other variety                             500        250

Fields of     the same variety not                  500        250
conforming      to  varietal  purity
requirements for Certification

Fields of Capsicum from Chilli and vice      500            250
versa

B.    Specific requirements

                 Factor                     Minimum permitted
                                               (%)*distance

                                          Foundation     Certified

                   1                          2              3

Off types                                    0.10           0.20

** Plants infected seed borne diseases       0.10           0.50

* Maximum permitted at and after flowering in the case of off types
and the final inspection in the case of seed borne diseases.

** Seed borne disease shall be: Leaf blight (Alternaria solani
Sorauer.); Anthracnose (Ripe rot, Die back) : (Collectotrichum
capsici (Syd.) Butler & Bisby)

V.    Seed standards

                 Factor                   Standard for each classes

                                          Foundation     Certified

                   1                          2              3

Pure seed (minimum)                         98.0%          98.0%

Inert matter (maximum)                      2.0%           2.0%

Other crops seeds (maximum)                  5/kg          10/kg

Weed seeds (maximum)                         5/kg          10/kg

Germination (minimum)                        60%            60%
Moisture (maximum)                      8.0%   8.0%

For vapour-proof containers (maximum)   6.0%   6.0%
OKRA (BHINDI) (Abelmoschus esculentus L. Moench)



I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Okra seed.

II.    Land requirements

       Land to be used for seed production of Okra shall be free of
       volunteer plants.
III.   Field Inspection

       A minimum of three inspections shall be made, the first before
       flowering, the second during peak flowering and fruiting stage and
       the third at mature fruit stage and prior to harvesting.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Okra Seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said table:

             Contaminants                  Minimum distance (meters)

                                           Foundation        Certified

                    1                           2                3

Fields of other variety                        500             250

Fields of the same variety not                 500             250
conforming     to    varietal    purity
requirements for Certification and wild
Okra (A. ficulneus (L.) Wt. & Arn. A.
manihot (L.) Medic. And A. moschatus
(L.) Medic.)




B.    Specific requirements

                Factor                     Minimum permitted (%)*

                                           Foundation     Certified

                   1                           2              3

Off types                                     0.10           0.20

** Objectionable weed plants                 None           None

* Maximum permitted at and after flowering.

** Objectionable weeds shall be wild Okra: (A.ficulneus (L.) Wt. &
Arn. A. amnihot (L.) Medic.,

A. moschatus (L.) Medic.)

V.    Seed standards

                Factor                     Standard for each classes

                                           Foundation     Certified

                   1                           2              3

Pure seed (minimum)                          99.0%          99.0%

Inert matter (maximum)                       1.0%           1.0%

Other crops seeds (maximum)                  None           None

Total Weed seeds (maximum)                   None           None

* Objectionable weed seeds (maximum)         None           None

Other    distinguishable       varieties     10/Kg          20/Kg
(maximum)
Germination   including   hard   seeds      65%               65%
(minimum)

Moisture (maximum)                         10.0%          10.0%

For vapour-proof containers (maximum)       8.0%              8.0%

* Objectionable weeds are the same as given at IV.B. above.
LETTUCE (Lactuca sativa L.)

I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of the Lettuce seed.

II.    Land requirements

       Land to be used for seed production of Lettuce    shall be free of
       volunteer plants.

III.   Field Inspection
       A minimum of three inspections shall be made, the first before
       heads have formed in heading types, and before full grown stage in
       on-heading types, the second when heads have formed in heading
       types, and at full grown stage in non-heading types and the third
       at flowering stage.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Lettuce seed fields shall be isolated from the contaminants shown
       in column 1 of the Table below by the distances specified in
       columns 2 and 3 of the said table:

             Contaminants                  Minimum distance (meters)

                                           Foundation        Certified

                    1                           2                3

Fields of other variety                        100              50

Fields of the same variety not                 100              50
conforming     to    varietal     purity
requirements for Certification and Wild
lettuce (Lactuca scariola L.)



B.    Specific requirements

                  Factor                    Minimum permitted (%)*

                                            Foundation     Certified

                     1                          2              3

Off types                                      0.10           0.20

** Objectionable weed plants                  0.010          0.020

*** Plants affected by seed borne disease      0.10           0.50

* Maximum permitted at the final inspection.

** Objectionable weeds shall be:wild Lettuce (Lactuca scariola L.)

V.    Seed standards

                  Factor                    Standard for each classes

                                            Foundation     Certified

                     1                          2              3

Pure seed (minimum)                           98.0%          98.0%

Inert matter (maximum)                        2.0%           2.0%

Other crops seeds (maximum)                   None           None

Total Weed seeds (maximum)                    5/Kg           10/Kg

* Objectionable weed seeds (maximum)          2/Kg           5/Kg

Other    distinguishable        varieties     10/Kg          20/Kg
(maximum)

Germination (minimum)                          70%            70%

Moisture (maximum)                            8.0%           8.0%
For vapour-proof containers (maximum)       6.0%              6.0%

* Objectionable weeds are the same as given at IV.B. above.
ONION (Allium cepa L.) HYBRIDS

I.    Application and Amplification of General Seed Certification
      Standards

A.    The General Seed Certification Standards are basic and, together
      with the following specific standards constitute the standards for
      certification of hybrid Onion seed.

B.    The General Standards are amplified as follows to apply specifically
      to the hybrids of Onion:

      1.    Eligibility requirements for certification

      (a)    An inbred line to be eligible for certification shall be from a
      source such that its identity may be assured and approved by the
      Certification Agency.

      (b)    Hybrid seed to be eligible for certification shall be the
      progeny of two approved inbred lines, one of which shall be male
      sterile.

      2.    Classes and Sources of seed

      (a)    An inbred line shall be a relatively true breeding strain
      resulting from self-pollination with selection.

      (b)    The Foundation class seed shall consist of an approved male
      sterile line to be used as a female parent and an approved inbred
      line to be used as a male parent for the purpose of producing
      hybrid seed.

      (c)   A male sterile line shall be a strain (A) carrying cytoplasmic
      genetic male sterility, which sheds no viable pollen and is
      maintained by the normal sister strain (B) which is used as
      pollinator.

      (d)   The Certified class seed shall be the hybrid seed to be
      planted for any use except seed production.

II.   Land requirements

      Land to be used for seed production of hybrid Onion shall be free
      of volunteer plants.
III.   Field Inspection

A.     Mother bulb production stage

       A minimum of two inspections shall be made as follows:

       1.    the first inspection shall be made after transplanting of
       seedlings in order to determine isolation, volunteer plants, off types
       including bolters and other relevant factors;

       2.    the second inspection shall be made after the bulbs have
       been lifted to verify the true characteristics of bulbs.

B.     Seed production stage
       A minimum of four inspections shall be made as follows:

       1.    the first inspection shall be made before flowering in order to
       determine isolation, volunteer plants, out crosses, planting ratio,
       errors in planting and other relevant factors;

       2.    the second and third inspections shall be made during
       flowering to check isolation, pollen shedding umbels, off types and
       other relevant factors;

       3.    the fourth inspection shall be made at maturity to verify the
       true nature of umbels and other relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields offered for certification shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3, 4 and 5 of the said table:

        Contaminants                     Minimum distance (meters)

                                      Mother bulb         Seed production

                                   production stage            stage

                                 Foundati    Certified   Foundati   Certified
                                   on                    on

               1                   2         3           4            5

Fields   of   other  variety       5         5        1200            600
including commercial hybrid
of the same variety

Fields of the same hybrids         5         5        1200            600
(code    Designation)    not
conforming to varietal purity
requirements for Certification

Fields of the other varieties of   -         -        1500            750
different skin colour

Fields of the other hybrids        5         5           -             -
having common male parent
and conforming to varietal
purity      requirements for
certification

B.    Specific requirements

                   Factor                     Minimum permitted

                                           Foundation          Certified

                     1                           2                3

* Bulbs not conforming to varietal           0.010%             0.050%
characteristics                            (by number)        (by number)

Off types (umbels) in seed parent at and     0.10%              0.050%
after flowering

Off types (umbels) in pollinator at and      0.010%             0.050%
after flowering
Pollen shedding (umbels) in seed parent    0.050%          0.10%
at flowering

* Maximum permitted at second inspection at mother bulb
production stage.



V.   Seed standards

                Factor                    Standard for each classes

                                          Foundation     Certified

                  1                           2              3

Pure seed (minimum)                         98.0%          98.0%

Inert matter (maximum)                      2.0%           2.0%

Other crops seeds (maximum)                 5/Kg           10/Kg

Weed seeds (maximum)                        5/Kg           10/Kg

Germination (minimum)                        70%            70%

Moisture (maximum)                          8.0%           8.0%

For vapour-proof containers (maximum)       6.0%           6.0%
       POTATO – TISSUE CULTURE –RAISED MINITUBER – (PTCMT)
                            Standard

I.  Application and Amplification of General Seed Standards for
PTCMT

A.      The General Seed Standards are basic and, together with the
        following specific standards constitute the standards for approval
        of PTCMT. As the name implies, these standards are applicable to
        tissue culture raised mini tubers multiplied under laboratory and
        greenhouse conditions as laid here.

B.      The General Standards are amplified as follows to apply specifically
        to the PTCMT:

1.      Eligibility requirements for PTCMT production:

        The PTCMT to be eligible for production shall be from a source
        meeting the following standards for laboratory and greenhouse
        facilities.

i.      Laboratory and greenhouse facilities used for production of
        plantlets/microtubers or minitubers shall be maintained free of
        potato pests or vectors of potato pathogens. Failure to keep such
        pests under control may cause rejection of all lots maintained in
        the facility. All potting or growth media shall be sterile. Water to be
        used in a laboratory or greenhouse operation should be free from
        impurities.

ii.     Hygienic   conditions   shall    be    strictly  observed     during
        micropropagation, potting, planting, irrigating, movement and use
        of equipment and other laboratory and greenhouse practices to
        guard against the spread of diseases or pests in the facilities used
        for seed multiplication.

iii.    All micro propagation and greenhouse facilities must be approved,
        as per the standard/guidelines. These facilities must have a
        changing area between the double doors.

iv.     The greenhouse (protected environment) must be “insect proof”
        and be equipped with a double-door entrance, provision for
        footweat disinfection prior to entering the protected environment
        and insect proof ventilation screening on intakes and exhaust
        openings. The persons entering the protected environment should
        use Wellington boots (Plastic boots) and change lab-coat in the
        changing area to reduce the chances of inadvertent introduction of
        vector insects clinging to clothes.

v.      The material being initiated for producing PTCMT must be of
        Registered/Notified variety1 and confirmed identity. It must be duly
        documented with respect to origin.

vi.     The plants of a potato varieties being initiated for tissue culture
        should be tested in an accredited laboratory2 for freedom from the
        following:
PVA, PVS, PVM, PVX,PLRV, PALCV, PSTVd and endophytic or epiphytic
bacteria and fungi. Tests must be carried on a minimum of ten plantlets
of each variety selected at random. For virus testing ELISA or an
equivalent method should be used, for viroid RT-PCR should be used,
and for fungi and bacteria light microscopy and culturing on media
should be used.

2.      Sources of seed:

(i)     The facility should use recognized aseptic initiation and
        propagation procedures (i.e. follow procedures and use equipment,
        which will maintain sterile conditions as per standard tissue
        culture norms.)

(ii)    The initiating facility must maintain following information on each
        variety for review and audit by the competent authority once in a
        year: variety identification, date of initiation, origin and testing
        results from accredited laboratory.

(iii)   Tests must be carried out on a minimum of ten plantlets, selected
        at random, for each variety by an accredited laboratory. No plant
        should contain PVA, PVS, PVM, PVY, PVX, PLRV, PALCV, PSTVd
        and other endophytic or epiphytic bacteria and fungi.

(iv)    Valid pathogen testing results are required prior to the initiation of
        micro tuber production cycle or planting of test tube plantlets in
        the greenhouse.
(v)     PTCMT shall be produced and multiplied from approved source in
        vitro plants or microtubers, as per the requirements.

(vi)    PTCMT may be used as breeder seed for further production
        certified classes of seed as prescribed in the Indian Minimum Seed
        Certification Standards.

(vii)   Concerned laboratory should issue a certificate to the effect that
        the PTCMT has been produced with the standards as prescribed
        under their supervision.

II.     Greenhouse/Controlled Environment Requirement

1.      All micropropagation and greenhouse facilities must meet the
        standards given above under eligibility requirements.

2.      The soil used for PTCMT production should not be infested with
        pathogen and pests of potato, particularly the following:

           wart (Synchtrium endobioticum (Schilb.) Perc.) and or cyst
            forming nematodes;

           brown rot (Pseudomonas solanacearum (E.F. Sm.) E.F. Sm. Or
            non-cyst forming nematodes within the previous three years;

           common scab (Streptomyces scabies (Thaxt.) Waks. & Henrici).

III.    Inspection of Greenhouse/Controlled Environment facility
        used for production of PTCMT

1.      The grower must notify the Competent Authority of his production
        plans well in advance of the planting.

2.      The crop must be grown from approved basic source in vitro plants
        or micro tubers, which were produced, in an aseptic environment.

3.      A minimum of three inspections shall be made as follows:

i.      The first inspection shall be made 35 days and 45 days after
        planting for plains and hills respectively to verify growing
        conditions, extent of disease infection and off types and also to
        confirm isolation requirement of one meter between different
        varieties as to avoid mechanical admixture.
ii.    The second inspections shall be made at 60-65 days after planting
       to verify off types, disease infection if any and pathogen testing, on
       a representative samples, comprising of 1% of the plants with a
       minimum of 5 and a maximum of 25 plants sampled for each
       variety;

iii.   The third inspection shall be made immediately after haulms
       cutting/destruction in order to verify that haulms have been
       cut/destroyed by the prescribed date and proper manner.

4.     Effective   sanitation   practices   including   insect   and   disease
       monitoring and prevention must be adhered to.
5.     Basic Stock can be planted in commercially available medium,
       which has not been recycled. If nursery beds are used, the
       substrate should be properly sterilized before planting.

6.     The greenhouse must be free from all potato and solanaceous plant
       debris before planting.

7.     No field-produced seed potatoes (including pathogen tested clonal
       selections), non-seed potatoes, nor any other solanaceous species
       of plants can be grown in the protected environment while used to
       produce Basic Stock.

8.     Varieties must be separated by appropriate partitioning of
       greenhouse to prevent varietal mixture.

9.     If testing performed by an accredited laboratory reveals the
       presence of banned virus (es), fungus or bacteria all the crops in
       the protected environment will be ineligible for multiplication and
       the entire material will be destroyed.

10.    In the eventuality of detection of insect (particularly aphids, thrips
       and white flies) vectors (for which yellow stricky traps should be
       put at least at three places in a greenhouse) by competent
       Authority, the grower must provide post harvest test results to this
       authority. A representative sample, representing each variety
       grown in the protected environment must be post harvest tested
       and if the results are negative for PVA, PVS, PVM, PVX, PLRV and
      PALCV, the crop will be assigned basic stock status or otherwise
      rejected.

IV.   Field Standards:

A.    Field Standards of PTCMT at greenhouse

      a.    General requirements

1.    Isolation: Minimum 1 meter between the different varieties grown
      in greenhouse so as to avoid mechanical admixture.

2.    All micropropagation and greenhouse facilities must be notified
      (approved) by DAC, as per the standards given above under
      eligibility requirements.
      b.    Specific requirements
                                           Maximum permissible limits
Factor
* Off types                                                     0.05%
** Plants showing symptoms of:
- Mild mosaic                                                   0.05%
- Severe mosaic, leaf roll, yellows                             0.05%
and apical leaf curl
** Plants infected by brown rot                                    nil
(syn. Bacterial wilt)
(Ralstonia solamacearum)

* Maximum permitted before dehaulming

** Maximum permitted at final inspection, though the diseases
mentioned above are not expected to be present in tissue culture
raised plants but it essential to maintain a good crop hygiene.

c.    Seed Standards for PTCMT

Factor                                    Standards for PTCMT

Weight of mini tuber (minimum)                    1.0gm

Germination/sprouting (minimum)                   90%

Varietal Purity (minimum)                         99%

Pure seed                                         98%
Virus                                                   0.01%

B.      Field standards for Foundation Crops and Certified Crop raised out
        of Potato-Tissue Culture raised Mini Tuber (PTCMT) (shall be same
        as prescribed for conventional method)3 at Annexure-III.
1In vitro multiplication for custom production of an imported variety or a
non-notified variety can be taken up by the industry exclusively for export
purposes. Such varieties, however, should be introduced following the
approved guidelines of Government of India.
2 The following laboratories of the ‘National Facility for Virus Diagnosts
and Quality Control of Tissue Culture Raised Plants’ are currently
accredited for virus testing: (1) Advanced Centre for Plant Virology, Indian
Agricultural Research Institute, New Delhi (2) Indian Institute of
Horticultural Research, Hessaraghatta Lake PO, Bangalore, (3) Institute of
Himalayan Bioresources Technology,2 Post Box. No.6, Palampur (HP). In
addition Central Potato Research Institute is also accredited. Of these four
laboratories, Advanced Centre for Plant Virology, Indian Agricultural
Research Institute will act as Referred lab.
3   Details not required here but given for reference
LABORATORIES FOR CERTIFICATION OF PTCMT                       – MINIMUM
REQUIREMENT



       Under the National Facility for Virus Diagnosis and Quality Control
of Tissue Culture Raised Plants, the following three centers are working
for the virus testing.

      1.   Indian Agricultural Research Institute, New Delhi (Referral
      Laboratory)

      2.    Indian Institute of Horticultural Research, Bangalore

      3.    Institute of Himalayan Bioresource Technology, Palampur
       All the three centers of the National Facility for Virus Diagnosis
and Quality Control of Tissue Culture Raised Plants have excellent infra-
structure facilities and welt trained and experienced scientists to test for
plant viruses using state-of the-art diagnostic techniques like ELISA,
PCR,      dot    blot   hybridization,   Western     blotting,    immuno-
electromicroscopy, etc. Considering the availability of expertise and
necessary infrastructure, these laboratories are accredited to test and
certify plants for freedom from viruses. Apart from these three
laboratories considering the availability of expertise and necessary
infrastructure for this work, the Central Potato Research Institute,
Shimla is also accredited for virus testing of PTCMT.

1.    Advanced Centre of Plant Virology, Indian Agricultural Research
      Institute, New Delhi-110012 (Referral Laboratory)

      The Centre was established in 1988 for characterization of plant
viruses and development of management practices. Since then, a large
number of viruses have been characterized, their diagnostic reagents
prepared and technologies developed for the management of important
viral diseases of plants. This center is well equipped with all the
necessary facilities required for virus work like ultra centrifuges,
electron-microscope, electrophoretic sytems, PCR machines, nucleic acid
sequencer, insect-proof glass houses, environment controlled glasshouse
meeting biosafety requirement and plant growth chambers. The Centre
has scientists trained in various laboratories in the USA and Europe on
different aspects of virology including molecular virology. It is a leading
National Centre for work on plant viruses.

2.    Indian Institute of Horticultural Research, Bangalore

      The Indian Institute of Horticultural Research has a very strong
unit for work on plant viruses affecting horticultural crops. It has the
basic facilities like ultra centrifuges, electromicroscope, electrophorotic
systems, PCR machine, etc. It also has a very good insect-proof glass
house facility for growing plants under controlled conditions. The
scientists working in the unit are very well trained in India and abroad.
The Centre is playing an important role in testing for viruses in
horticultural crops.
3.    Institute of Himalayan Bioresources Technology, Bangalore

       Institure of Himalayan Bioresources Technology has also developed
excellent facility for work on plant viruses. It has developed diagnostic
technique for testing of viruses affecting horticultural plants particularly
the ornamentals. The Centre has good with ultracentrifuges,
eelectrophorotic systems, PCR machines, ELISA reader, Sequences, etc.
The Centre has a good team of scientists trained in India and abroad.
Considering the above capabilities, it would be useful to identify all the
three centers as testing centers for the purpose on certification for
freedom from viruses.

4.    Central Potato Research Institute, Shimla.

Central Potato Research Institute, Shimla was established in 1949 to
improve potato production in the country through intensive multi
disciplinary research and production of healthy seed potato. As a result
of technologies developed at this institute potato production has
increased by nearly 15 times and potato productivity 2.67 times, making
India the fourth largest producer of potato. The Institute has developed
excellent facilities and has highly competent scientists for virus testing of
PTCMT. The facilities available at the Institute include electron
microscope, ultracentrifuges, electrophoritic systems, PCR machines,
EUSA reader, nucleic acid hybridization facility etc. They also have well
maintained insect-proof glasshouses. The scientists of the Institute have
been trained in India and abroad.
General Requirements for accrediting a laboratory for virus testing

1.     The laboratory must be adequately equipped for virus diagnostic
       work. It must have basic equipment like ultracentrifuge,
       electrophoritic system, PCR machine, ELISA reader, etc.

2.     The laboratory must have facilities for growing plants under insect-
       proof conditions.

3.     The laboratory must have at least two scientists with good training
       in virology, preferably Ph.D in virology, and experience of working
       on virus diagnosis.



          Labelling of Potato – Tissue Culture Mini tuber (PTCMT)

      PTCMT shall be supplied in sealed containers. A cloth-lined label of
12 cm x 6 cm containing following information shall be affixed on the
container.

                                                   Label No.

Crop                      Potato

Variety

Class of seed             PTCMT

Lot No.

Approved     laboratory
and reference:

Date of test

Germination/sprouting %
(Minimum)

Producing Agency
(Name and address)

     “The container should also have printed on it the kind, variety and
name of Institute’.
2.    The label shall be rubber stamped with signature, name and
designation of the concerned Agency. Colour of the label shall be
diagonally yellow No.356 (IS 5-1978) and opaline green (IS No.275).



3.   PTCMT (Breeder seed) producing Agency shall maintain the
account of labels printed and issued.
Seed Certification Standards for Potato Tissue Culture raised
Minitubers (PTCMT)



I.     Application and Amplification of General Seed Certification
       Standards

       A. The General Seed Certification Standards are basic and,
          together with the following specific standards constitute the
          standards for certification of seed potato.

       B. Classification of seed potato on the basis of area of Production:

            There shall be two types of seed ptatoes, namely the Hills and
            Plains – grown and shall be designated as Hill (HS) and Plains
            Seed (PS) respectively. Hill Seed (HS) shall be grown in the high
            hills generally 2500 meters above the mean sea level or in
            situations declared technically suitable for seed production.
            Plains Seed (PS) shall be grown in such areas where aphid
            infestation is low during the crop growing season and which are
            technically suitable for seed production.

II.    Land Requirements

       A crop of seed shall not be eligible for certification if grown on land
       infested with:

       -       Wart (Synchytrium endobioticum (Schilb.) Perc. And or cyst
               forming nematodes;

       -       Brown rot (Pseudomonas solanacearum (E.F. Sm.). E.F. Sm.
               Or non-cyst forming nematodes within the previous three
               years;

       -       Common scab (Streptomyces scabies (Thaxt.) Waks. &
               Henrici).

III.   Field Inspection

       A minimum of four inspections shall be made as follows:

       1.      the first inspection shall be made about 45 days after
               planting the PTCMT in the hills and about 35 days after
             planting the PTCMT in the plains to verify isolation, off types
             and the extent of disease infection with specific reference to
             mild and severe mosaics, leaf roll, yellow, brown rot and
             other relevant factors;

      2.     the second inspection shall be made about 60-65 days
             planting the PTCMT for early varieties and about 70-75 days
             after planting the PTCMT for late varieties or at appropriate
             growth stage depending on the crop duration of the variety
             concerned to check isolation, off types and extent of disease
             infection with specific reference to mild and severe mosaics,
             leaf roll, yellows, brown rot and other relevant factors;
      3.     the third inspection shall be made immediately after haulms
             cutting/destruction in order to verify that haulms have been
             cut/destroyed by the prescribed date and in proper manner;

      4.     the fourth inspection shall be made about 10 days after
             haulms cutting/destruction and before harvesting in order to
             verify that no re-growth of haulms has taken place.

IV.   Field Standards

      A.     General Requirements

      1.     Isolation

             The fields of seed potato shall be isolated from the
             contaminants shown in column 1 of the Table below by the
             distances specified in columns 2, 3 and 4 of the said Table:

           Contaminants                 Minimum distance (meters)

                                         Foundation            Certified

                                     Stage-I     Stage-II

                 1                      2            3             4

Fields of other varieties               5            5             5

Fields of the same hybrid not           5            5             5
conforming to Varietal purity
 requirements for Certification

B.    Specific Requirements
                                  Maximum permissible limits
Factor             Stage          Stage-I    Stage-II      Stage-III
1                  2              3          4             5
Off types               I & II      0.050%     0.050%         0.10%
                     Inspection
Plants                  I & II       1.0%         2.0%         3.0%
showing              Inspection
symptoms of:

- Mild mosaic
-Severe                I & II       0.50%        0.75%         1.0%
mosaic,     leaf    Inspection
roll        and
yellows
*Total Virus             -           1.0%         2.0%          3.0%
**       Plants        I & II        None         None      3 Plants per
infected      by    Inspection                                hectare
brown        rot
(Syn.
Bacterial wilt)
(Pseudomonas
solanacearum
(E.F.      Sm.)
E.F. Sm.)
***Re-growth            IV          0.50%        0.50%        0.50%
of plants after     Inspection
destruction of
haulms
* Of the two inspections, the higher virus percentage will be
considered for the purpose of the specified limits of tolerance.
** The presence of brown rot infected plants within the specified
limits of tolerance shall be permitted in the areas known to be
infected with this disease. In case of plants suspected to be
infected with brown rot, the neighbouring plants, one on either
side should also be rogued along with tubers.
*** Standards for re-growth after destruction of haulms shall be
met at fourth inspection to be conducted about 10 days after
haulms cutting.
     Note: 1. All off types and diseased plants should be rouged out along
           with the tubers
           and destroyed.
        2. Gaps in the seed plot should not be more than 10.0%.
        3. Haulms must be destroyed as close to the ground as possible
           before the date specified by the Certification Agency. Failure to
           destroy haulms in time shall render the crop liable for rejection.
V.      Seed Standards

A.      Specification in respect of size and weight of seed material for
        Foundation Stage-I, Foundation Stage-II and Certified class shall
        be as under:

           Size              Mean length and two      Corresponding weight
                            widths At the middle of
                                    tuber

(a) Hill Seed (HS)
     Seed Size                 30 mm – 60 mm               25 – 150 gm
     Large size                 above 60 mm               above 150 gm

(b) Plains Seed (PS)
     Seed Size                 30 mm – 55 mm               25 – 125 gm
     Large size                 above 55 mm               above 125 gm

Note:

        1. The size of tuber will be decided either on the basis of mean of
           two widths of a tuber at the middle and that of length or on the
           basis of corresponding weight of tuber.

        2. In a seed lot, tubers not conforming to specific size of seed shall
           not exceed more than 5.0% (by number).

        3. (a) The seed material shall be reasonably clean, healthy, firm
           and shall conform to the characteristics of the variety. The
           tubers not conforming to the varietal characteristics shall not
           exceed 0.050% and 0.10% (by number) for Foundation and
           Certified seed classes respectively.
        (b) Cut, bruised, unshapy, cracked tubers or those damaged by
        insects, slugs or worms shall not exceed more than 1.0% (by
        weight).

        (c) Greenish pigmentation on           tubers    will   not       be   a
        disqualification for certification.

B.   Maximum tolerance limit of tubers showing visible symptoms
     caused by the diseases mentioned below will be as follows:

                                       Minimum permissible limits

           Diseases                     Foundation               Certified

                                     Stage-I     Stage-II

               1                       2            3                 4

Late     blight  )Phytophthora        1.0%        1.0%             1.0%
infestations (Mont.) de Bary),         (by         (by          (by number)
dry rot (Fusarium caeruleum          number)     number)
(Lib.) Sacc.) or Charcoal rot
(Macrophomina phaseoli (Tassi)
G. Goidanich).

Wet rot     (Sclerotium   rolfsii)    None        None             None
Sacc.)

* Common sacb (Streptomyces           3.0%        3.0%             3.0%
scabies  (Thaxt) Waks.    &            (by         (by          (by number)
Henrici)                             number)     number)

** Black scurf       (Rhizoctonia     5.0%        5.0%             5.0%
solani Kuehn.)                         (by         (by          (by number)
                                     number)     number)

*** Total diseases                    5.0%        5.0%             5.0%
                                       (by         (by          (by number)
                                     number)     number)

* Even if a single tuber infected with common scab is detected in a
seed lot, the entire seed lot shall be treated with approved
fungicide before seed lot is declared fit for certification. Seed lots
having infected tubers more than the prescribed limits will not be
certified even after treatment.

** (a) A tuber carrying 10.0% or above scurfed surface will be
considered as one infected unit.

  (b) Seed lots having black scruf infection more than the prescribed
      limits could be certified after treatment with approved
      chemical/fungicide.

  (c) For all diseases, the higher disease percentage will be considered
      for the purpose of the specified limits of tolerance.
3. FLOWERS
ASTER (Callistephus spp. (Linn.) Nees syn. (Aster sp.. Linn.)

I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and together
       with the following specific standards - constitute the standards for
       certification of Aster.

II.    Land requirements

       Land to be used for seed production of Aster shall not be eligible
       for certification if planted on land on which the same kind of crop
       (including Aster – singles) was grown in the previous year unless
       the crop(s) as grown in the previous year was/were of the same
       variety and of an equivalent or higher class of certified seed and
       was/were certified.

III.   Field Inspection

       A minimum of three inspections shall be made, the first at pre-
       flowering stage, the second during flowering and the third before
       (harvesting) pappus or “fuzz” begins to show.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Aster seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said table:

             Contaminants                   Minimum distance (meters)

                                            Foundation          Certified

                     1                            2                3

Fields of other varieties                       600               300

Fields of     the same variety not              600               300
conforming      to  varietal  purity
requirements for Certification

B.    Specific requirements

                 Factor                   Minimum permitted (%)*

                                         Foundation          Certified

                   1                          2                 3

Off types                                   0.050              0.10

* Standards for off types shall be met at after flowering.

V.    Seed standards

                 Factor                  Standard for each classes

                                         Foundation          Certified

                   1                          2                 3

Pure seed (minimum)                        98.0%              98.0%

Inert matter (maximum)                      2.0%               2.0%

Other crops seeds (maximum)                10/Kg              20/Kg

Weed seeds (maximum)                        5/Kg              10/Kg

Germination (minimum)                       60%                60%

Moisture (maximum)                          9.0%               9.0%

For vapour-proof containers (maximum)       6.0%               6.0%
ANNUAL CARNATION (MARGURITE OR CHAUBAD TYPE) (Dianthus
Caryophyllus Linn.)



I.       Application and Amplification of General Seed Certification
         Standards

         The General Seed Certification Standards are basic and, together
         with the following specific standards constitute the standards for
         certification of the seeds of Annual Carnation.
II.      Land requirements

         Land to be used for seed production of Annual Carnation shall be
         free of volunteer plant.

III.     Field Inspection

         A minimum of three inspections shall be made, the first after
         transplanting, the second during flowering and the third before
         (harvesting) capsules start opening (shattering).

IV.      Field Standards

         A.        General requirements

         1.        Isolation

         Annual Carnation seed fields shall be isolated from the
         contaminants shown in column 1 of the Table below by the
         distances specified in columns 2 and 3 of the said table:

                   Contaminants                  Minimum distance (meters)

                                                 Foundation     Certified

                          1                          2              3

Fields of other varieties                           600           300

Fields        of    the   same   variety   not      600           300
conforming     to     varietal    purity
requirements for Certification

B.    Specific requirements

                Factor                     Minimum permitted (%)*

                                           Foundation     Certified

                   1                           2              3

Off types                                    0.050           0.10

** Plants affected by designated disease      0.10           0.20

* Standards for off types shall be met at and after flowering and for
designated disease at final inspection.

** Designated disease shall be

Streak mosaic virus.

V.    Seed standards

                Factor                     Standard for each classes

                                           Foundation     Certified

                   1                           2              3

Pure seed (minimum)                          97.0%          97.0%

Inert matter (maximum)                       3.0%           3.0%

Other crops seeds (maximum)                  10/Kg          20/Kg

Weed seeds (maximum)                         5/Kg           10/Kg

Germination (minimum)                         75%            75%

Moisture (maximum)                           8.0%           8.0%

For vapour-proof containers (maximum)        6.0%           6.0%
SNAPDRAGON (Antirrhinum spp. Linn.)



I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of Snapdragon seeds.

II.    Land requirements

       Land to be used for seed production of Snapdragon shall be free of
       volunteer plant.
III.   Field Inspection

       A minimum of three inspections shall be made, the first before
       flowering, the second during flowering and the third at maturity
       prior to harvesting.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields of Snapdragon shall be isolated from the contaminants
       shown in column 1 of the Table below by the distances specified in
       columns 2 and 3 of the said Table:

             Contaminants                  Minimum distance (meters)

                                           Foundation        Certified

                     1                          2                3

Fields of other varieties                      600             300

Fields of the same variety not                 600             300
conforming     to     varietal purity
requirements for Certification
B.    Specific requirements

                  Factor                     Minimum permitted (%)*

                                             Foundation     Certified

                     1                           2              3

*Off types                                     0.050           0.10

** Plants      affected    by   designated      0.10           0.20
diseases

* Standards for off types shall be met at and after flowering and for
designated diseases at final inspection.

** Designated diseases shall be:

Anthracnose (Colletotrichum antirrhini & C. fuscum)

Blight (Phyllosticta antirrhini_Syd.)

V.    Seed standards

                  Factor                     Standard for each classes

                                             Foundation     Certified

                     1                           2              3

Pure seed (minimum)                            98.0%          98.0%

Inert matter (maximum)                         2.0%           2.0%

Other crops seeds (maximum)                    10/Kg          20/Kg

Weed seeds (maximum)                           5/Kg           10/Kg

Germination (minimum)                           70%            70%
Moisture (maximum)                      8.0%   8.0%

For vapour-proof containers (maximum)   6.0%   6.0%
SNAPDRAGON HYBRIDS (Antirrhinum spp. Linn.)



I.   Application and Amplification of General Seed Certification
     Standards

A.   The General Seed Certification Standards are basic and, together
     with the specific standards constitute the standards for
     certification of hybrid snapdragon seeds.

B.   The General Standards are amplified as follows to apply specifically
     to the hybrids of Snapdragon.

1.   Eligibility requirements for certification
a.   An inbred/parental line to be eligible for certification shall be from
     a source that its identity may be assured and approved by the
     certification agency.

b.   *Hybrid seed to be eligible for certification shall be the progeny of
     two approved inbred/parental lines, one of which shall be male
     sterile.

2.   Classes and Sources of seed

a.   An inbred/parental line shall be a relatively true breeding strain
     resulting from self-pollination with selection and/or developed
     through in-vitro culture.

b.   he foundation seed class shall consist of an approved male sterile
     line (genetic male sterility-apetalous sterile line) to be used as a
     female parent and an approved inbred line to be used as a male
     parent for the purpose of producing hybrid seed.

c.   *A male sterile line shall be a monogenic recessive strain carrying
     genetic male sterility, which sheds no viable pollen and is
     maintained by the monogenic dominant heterozygous strain and is
     used a maintainer in propagation blocks.

d.   The certified class seed shall be hybrid seed to be planted for any
     use except seed production.
     (*Applicable if male sterile line is used)
II.    Land requirements

       Land to be used for seed production of Snapdragon shall be free of
       volunteer plant.

III.   Field Inspection

A.     Foundation and certification seeds.

       A minimum of three inspections shall be made as follows:

1.     The first inspection shall be made before flowering in order to
       determine the isolation, volunteer plants, outcrosses, and other
       relevant factors.
2.     The second inspection shall be made during flowering / podding
       stage to check isolation, off types, fertile segregants in seed parent
       and other relevant factors.

3.     The third inspection shall be made at pod maturity and prior to
       harvesting to check off types, the designated diseases and other
       relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields of Snapdragon hybrids shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said Table:

                 Contaminants                Minimum distance (meters)

                                             Foundation         Certified

                           1                       2                3

       Fields of other varieties including        600              300
       Hybrids of the same cultivar

       Fields of the same variety not             600              300
       conforming to varietal purity
       requirements for Certification

B.    Specific requirements

                      Factor                Minimum permitted (%)*

                                           Foundation      Certified

                         1                       2             3

       Off types in seed parent               0.050           0.10

       Off types in pollinator                0.050           0.10

       Fertile   segregants      (Pollen      0.050           0.10
       shedding spikes in seed parent)

       ** Plants affected by designated        0.10           0.20
       diseases

* Standards for off types and fertile segregants (pollen shedding
spikes) in seed parent shall be met at and after flowering and for
designated diseases at final inspection.

** Designated diseases shall be:

Anthracnose (Colletotrichum antirrhini & C. fuscum)

Blight (Phyllosticta antirrhini_Syd.)

V.    Seed standards

                  Factor                    Standard for each classes

                                           Foundation      Certified

                     1                           2             3

Pure seed (minimum)                           98.0%          98.0%

Inert matter (maximum)                         2.0%          2.0%

Other crops seeds (maximum)                   10/Kg          20/Kg

Weed seeds (maximum)                           5/Kg          10/Kg

Germination (minimum)                          70%            70%
Moisture (maximum)                      8.0%   8.0%

For vapour-proof containers (maximum)   6.0%   6.0%
ANNUAL CHRYSANTHEMUM (Chrysanthemum spp. Linn. )
I.     Application and Amplification of General Seed Certification
       Standards
       The General Seed Certification Standards are basic and, together
       with the      specific standards constitute the standards for
       certification of Annual Chrysanthemum.
II.    Land requirements
       A seed crop of Annual Chrysanthemum shall not be eligible for
       certification if planted on land on which the same kind of crop was
       grown in the previous year unless the crop (s) of Annual
       Chrysanthemum grown in the previous year was / were of the
       same variety and of an equivalent or higher class of certified seed
       and was / were certified.
III.   Field Inspection
       A minimum of three inspections shall be made as under:
       1.   The first inspection shall be made at pre – flowering stage in
            order to determine land requirements, isolation, volunteer
            plants, and other relevant factors.
       2.   The second inspection shall be made during flowering to
            verify relevant factors;
       3.   The third inspection shall made at maturity prior to
            harvesting.
IV.    Field Standards
       A.    General requirements
       1.    Isolation
             Annual Chrysanthemum seed fields shall be isolated from
             the contaminants shown in column 1 of the Table below by
             the distances specified in columns 2 and 3 of the said Table:

                 Contaminants              Minimum distance (meters)

                                            Foundation        Certified

                         1                       2                3

       Fields of other varieties                600             300

       Fields of the same variety not           600             300
       conforming to varietal purity
       requirements for Certification
B.    Specific requirements

                      Factor                    Minimum permitted (%)*

                                            Foundation         Certified

                         1                          2              3

         Off types at and after flowering         0.050           0.10

         * Plants affected by designated          0.10            0.20
         diseases

* Designated diseases shall be:

Grey mould (Botrytis cinerea Pers.)

Blotch        (Septoria chrysanthmella Sacc.)

V.    Seed standards

                   Factor                       Standard for each classes

                                            Foundation         Certified

                     1                              2              3

Pure seed (minimum)                              98.0%           98.0%

Inert matter (maximum)                            2.0%           2.0%

Other crops seeds (maximum)                      10/Kg           20/Kg

Weed seeds (maximum)                              5/Kg           10/Kg

Germination (minimum)                             50%             50%

Moisture (maximum)                                8.0%           8.0%

For vapour-proof containers (maximum)             6.0%           6.0%
PETUNIA (Petunia spp.)

I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the      flowering specific standards constitute the standards
       for certification of hybrid      seeds.

II.    Land requirements

       Land to be used for seed production of Petunia shall be free of
       volunteer plant.
III.   Field Inspection

       A minimum of two inspections shall be made the first before
       flowering / during flowering to check isolation off types etc. and
       second at fruiting state / maturity prior to harvesting.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields of petunia hybrids shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said Table:

                 Contaminants              Minimum distance (meters)

                                            Foundation        Certified

                         1                       2                3

       Fields of other varieties                600             300

       Fields of the same variety not           600             300
       conforming to varietal purity
       requirements for Certification
B.    Specific requirements

               Factor                        Minimum permitted (%)*

                                         Foundation            Certified

                  1                             2                 3

Off types                                   0.050                0.10

** Plants affected by designated             0.10                0.20
diseases

* Standards for off types shall be met at and after flowering and
for designated diseases at final inspection.

** Designated diseases shall be:

Leaf bright   (Alternaria alternate (Fr.) Kiessler)

Crown rot     (Phytophthora parasitica Dast.)

V.    Seed standards

                  Factor                            Minimum for each class

                                                Foundation        Certified

                      1                                2                3

Pure seed (minimum)                                   98.0%        98.0%

Inert matter (maximum)                                2.0%            2.0%

Other crops seeds (maximum)                           10/Kg        20/Kg

Weed seeds (maximum)                                  5/Kg         10/Kg

Germination (minimum)                                 75%             75%

Moisture (maximum)                                    8.0%            8.0%
For vapour-proof containers (maximum)   6.0%   6.0%
PETUNIA HYBRID (Petunia spp.)

I.      Application and Amplification of General Seed Certification
        Standards

A.      The General Seed Certification Standards are basic and, together
        with the      specific standards constitute the standards for
        certification of Petunia hybrid seeds.

B.      The General Standards are amplified as follows to apply specifically
        to the hybrids of Petunia.

1.      Class and source of seed

a.      Only the class “Certified” shall be recognized.
b.      A Hybrid to be certified must be produced from certified foundation
        seed or seed stocks approved by the certification agency.

2.      Eligibility requirements for certification

a.      A hybrid is one to be planted for any used except seed production.
        It may be one of the following:

(i)     Single cross

       The first generation resulting from controlled crossing of two
approved self – incompatible but cross – compatible inbred lines. It may
be of three types depending upon procedure of seed production.

        -     Seed of only female percent are harvested and certified

        -     Seeds of both the parents are harvested separately and
              certified and

        -     Seed of both the parents are harvested together, mixed and
              certified.

(ii)    Double cross

        The first generation resulting from the controlled crossing of two
        approved self –    incompatible but cross – compatible single
        crosses.

(iii)   Three way cross
       The first generation resulting from controlled crossing of an
       approved inbred line and certified foundation single cross being
       self – incompatible individually but cross –compatible to each
       other.

II.    Land requirements

       a)   Land to be used for seed production of petunia hybrids shall
            be free of volunteer plants.

III.   Field Inspection

       A minimum of three inspections shall be made as follows:

       1.   The first inspection shall be made at pre – flowering stage
            check isolation, out crosses and other relevant factors;

       2.   The second inspection shall be made during flowering to
            check isolation, off types and other relevant factors;

       3.   The third inspection shall be made before harvesting to
            check off types, the designated diseases and other relevant
            factors.

IV.    Field Standards

       A.   General requirements

       1.   Isolation

       Seed fields of Petunia hybrids shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said Table:

             Contaminants                  Minimum distance (meters)

                                           Foundation       Certified

                    1                           2                 3

 Fields of other varieties including           600                400
 commercial    Including  commercial
 hybrids of same cultivar

 Fields of the same variety not                600                400
 conforming  to  varietal  purity
requirements for Certification

Between blocks of the parental lines in              -                   005
case seed parent and pollinator are
planted in separate blocks.

B.    Specific requirements

                  Factor                        Minimum permitted (%)*

                                               Foundation          Certified

                        1                            2                    3

Off types in each parent                          0.050                  0.10

** Plants      affected     by   designated        0.10                  0.20
diseases

* Standards for off types shall be met at and after flowering and
plants infected with designated diseases at final inspection.

** Designated diseases shall be:

Leaf blotch                      (Cercospora petuniae (saito) Muller &)

Leaf spot                        (Ascochyta petuniae)

Phyllostica leaf spot            (Phyllostica petuniae Speg.)

Leaf bright                      (Alternaria alternate (Fr.) Kiessler)
Crown rot                        (Phytophthora parasitica Dast)

Tobacco mosaic virus (TMV)

Cucumber mosaic virus (CMV)

V.    Seed standards

                 Factor                         Standard for each classes

                                               Foundation          Certified

                    1                                2                    3
Pure seed (minimum)                     98.0%   98.0%

Inert matter (maximum)                  2.0%    2.0%

Other crops seeds (maximum)             10/Kg   20/Kg

Weed seeds (maximum)                    5/Kg    10/Kg

Germination (minimum)                   75%     75%

Moisture (maximum)                      8.0%    8.0%

For vapour-proof containers (maximum)   6.0%    6.0%
ORNAMENTAL SUNFLOWER (Helianthus spp. L)

I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the       specific standards constitute the standards for
       certification of Ornamental    Sunflower seeds.

II.    Land requirements

       A seed crop of ornamental sunflower shall not be eligible for
       certification if  planted on land on which the same kind of crop
       was grown in the previous year         unless the crop (s) grown in
       the previous year was / were of the same variety and of             an
       equivalent or higher class of certified seed and was / were certified.

III.   Field Inspection

       A minimum of three inspections shall be made as follows:

1.     The first inspection shall be made at the stage of 6-7 pairs of leaves
       in order to determine isolation, volunteer plants, designated
       disease and other relevant factors.

2.     The second inspection shall be made during flowering to check
       isolation, off types and other relevant factors;

3.     The third inspection shall be made at maturity and prior to
       harvesting to verify designated diseases, true nature of plant and
       head, characteristics of seed and other relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Ornamental sunflower seed fields shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said Table:
         Contaminants                Minimum distance (meters)

                                    Foundation          Certified

                 1                       2                 3

Fields of other varieties                400              200

Fields of the same variety not           400              200
conforming to varietal purity
requirements for Certification



B.   Specific requirements

                 Factor                      Minimum permitted (%)*

                                          Foundation       Certified

                     1                          2               3

*Off types at and after flowering              0.10            0.20

 Plants infected with downy mildew             0.050         0.050
diseases (Plasmopara halstdii (Feri.)
Beri & De T.) at each inspection

Plants infested with Orobanchi cumana          None            None
Guss, Non- Wallr. At final inspection.

* Sterile plants of the same variety shall not be considered as off
types.
V.   Seed standards

               Factor                   Standard for each classes

                                        Foundation     Certified

                  1                         2              3

Pure seed (minimum)                       98.0%          98.0%

Inert matter (maximum)                     2.0%          2.0%

Huskless seeds (maximum)                (By number)   (By number)

                                           None          None

Seeds (maximum)                            5/Kg          5/Kg

Seeds infested with Orobanche cumana       None          None
guss Non – wallr (maximum),

Germination (minimum)                      70%            70%

Moisture (maximum)                         9.0%          9.0%

For vapour-proof containers (maximum)      7.0%          7.0%
ORNAMENTAL SUNFLOWER (Helianthus spp. L.) Hybrid



I.    Application and Amplification of General Seed Certification
      Standards

A.    The General Seed Certification Standards are basic and together
      with the following specific standards constitute the standards for
      certification of  Ornamental sunflower hybrid seeds.

B.    The General Standards are amplified as follows to apply specifically
      to the hybrids of ornamental sunflower.
1.    Eligibility requirements for certification

a.    An hybrid line to be eligible for certification shall be from a source
      such that its identify may be assured and approved by the
      Certification Agency.

b.    Hybrid seed to be eligible for certification shall be the progeny of
      two approved inbred lines, one of which shall be made sterile.

2.    Classes and Sources of seed

a.    An inbred line shall be a relatively true breeding strain resulting
      from self-pollination with selection.

b.    The foundation seed class shall consist of an approved male sterile
      line to be used as a female parent and approved inbred line to be
      uses as a male parent for the purpose of producing hybrid seeds.

c.    A male sterile shall be a strain (A) carrying cytoplasmic – genetic
      male sterility, which sheds no viable pollen and is maintained by
      the normal sister strain (B) which is used as pollinator.

d.    The certified class seed shall be the hybrid seed to be planted for
      any use except seed production.

II.   Land requirements

A seed crop of ornamental sunflower hybrid shall not be eligible for
certification if planted on land on which the same kind of crop was grown
in the previous year unless the crop (s) grown in the previous year was of
the same variety and of an equivalent or higher class of certified seed and
was / were certified.

III.   Field Inspection

       A minimum of four inspections shall be made as follows:

       1.   The first inspection shall be made at the stage 6-7 pairs of
            leaves in order to determine isolation, out crosses, volunteer
            plants, planting ration, errors in planting, designated disease
            and relevant factors;

       2.   The second and third inspection shall be made during
            flowering to check isolation, pollen shedding heads, off types
            and other relevant factors;

       3.   The fourth inspection shall be made at maturity and prior to
            harvesting to verify designated diseases, true nature of plant
            and head, characteristics of seeds and other relevant factors.

IV.    Specific requirements

       A.   General requirements

       1.   Isolation

                 Contaminants                Minimum distance (meters)

                                             Foundation       Certified

                        1                        2                3

       Fields of other varieties including      600              400
       commercial                Including
       commercial hybrids of         same
       variety

       Fields of the same hybrid (code          400              200
       designation) not conforming to
       varietal purity requirements for
       certification and wild Helianthus
       spp.
B.   Specific requirements

                   Factor                  Minimum permitted (%)*

                                           Foundation     Certified

                      1                        2              3

      *Off types in each parent at and        0.20          0.50
      after flowering

      Off types in pollinator at and          0.20          0.50
      after flowering

      Pollen shedding heads in seed           0.50          1.00
      parent at flowering

      **Objectionable weed plants at         None           None
      and after flowering

      Plants infected with downy             0.050          0.50
      mildew   disease (Plasmopara
      halstedii (Farl.) Barl & de T.) at
      each inspection.

      Plants infested with Orobanche         None           None
      cumana Guss Non – Wallr.) at
      final inspection

* Sterile plants of the same strain shall no be considered as off

** Objectionable weed plants shall be: Wild Helianthus spp.
V.   Seed standards

               Factor                    Standard for each classes

                                        Foundation          Certified

                  1                          2                 3

Pure seed (minimum)                        98.0%             98.0%

Inert matter (maximum)                      2.0%              2.0%

Huskless seeds (maximum)                    2.0%              2.0%
                                        (By number)     (By number)
                                           None               None

Other crop seeds (maximum)                 None               None

Total weed seeds (maximum)                 5/Kg              10 /Kg

*Objectionable weed seeds (maximum)        None               None

Seeds infested with Orchanche cumana       None               None
Guss Non- Wallr. (maximum)

Germination (minimum)                       70%               70%

Moisture (maximum)                          9.0%              9.0%

For vapour-proof containers (maximum)       7.0%              7.0%

*Objectionable weed is the same as given at IV. B. above.
MARIGOLD (Tagetes spp. Linn.)

I.     Application and Amplification of General Seed Certification
       Standards

       The General Seed Certification Standards are basic and, together
       with the     specific standards are basic and, together with the
       Specific standards constitute the standards for certification of
       Marigold seeds.

II.    Land requirements

       A seed crop of Marigold shall not be eligible for certification if
       planted on land on        which the same kind of crop was grown in
       the previous year unless the crop (s)        grown in the previous
       year was / were of the same variety and of an equivalent or higher
       class of certified seed and was / were certified.

III.   Field Inspection

       A minimum of three inspections shall be made as follows:

1.     The first inspection shall be made at the stage of 6-7 pairs of leaves
       in order to determine isolation, volunteer plants and other relevant
       factors.

2.     The second inspection shall be made during flowering to check
       isolation, off types and other relevant factors;

3.     The third inspection shall be made at maturity to verify designated
       diseases, true nature of plant and other relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Marigold seed fields of shall be isolated from the contaminants
       shown in column 1 of the Table below by the distances specified in
       columns 2 and 3 of the said Table:
                  Contaminants                      Minimum distance (meters)

                                                    Foundation         Certified

                          1                               2                3

Fields of other varieties                                600              300

Fields       of   the     same      variety   not        600              300
conforming     to    varietal              purity
requirements for Certification



B.    Specific requirements

                        Factor                        Minimum permitted (%)*

                                                    Foundation         Certified

                          1                               2                3

Off types at and after flowering                        0.050             0.10

*Plants affected by designated disease                  0.10              0.20
at final inspection.

*Designated diseases shall be:

Leaf spot                        (Alternaria tagetica shome & Mustafee)

Flower bud rot                   (A. alternate (Fr.) Keissler & A. dianthi Stevans &
Hall)

Collar rot                       (Rhizoctonia solani Kuhn)
V.   Seed standards

               Factor                   Standard for each classes

                                        Foundation     Certified

                  1                         2              3

Pure seed (minimum)                       97.0%          97.0%

Inert matter (maximum)                    3.0%           3.0%

Other crops seeds (maximum)               10/kg          20/ kg

Weed seeds (maximum)                      5/Kg           10/Kg

Germination (minimum)                      70%            70%

Moisture (maximum)                        9.0%           9.0%

For vapour-proof containers (maximum)     7.0%           7.0%
MARIGOLD HYBRID (Tagetes spp.Linn)

I.   Application and Amplification of General Seed Certification
     Standards

A.   The General Seed Certification Standards are basic and, together
     with the      specific standards constitute the standards for
     certification of hybrids Marigold  seeds.

B.   General Standards are amplified as follows to apply specifically to
     the   hybrids      of Marigold.

1.   Eligibility requirements for certification
     a.    An inbred line to be eligible for certification shall be from a
           source such that its identity may be assured and approved
           by the certification Agency.

     b.    Hybrid seed to be eligible for certification shall be the
           progeny of two approved inbred lines, one of which shall be
           male sterile.

2.   Class and source of seed

     a.    An inbred line shall be a relatively true breeding strain
           resulting from self-pollination with selection and/or
           developed through in-vitro culture.

     b.    The foundation seed class shall consist of an approved male
           sterile (apetalous) line to be used as a female parent and an
           approved inbred line to be used as a male parent for the
           purpose of producing hybrid seed.

     c.    *A male sterile line shall be a monogenic recessive strain
           carrying genetic male sterility, which sheds no viable pollen
           and is maintained by the monogenic cominant heterozygous
           strain and is used as maintainer in propagation blocks.

     d.    The certified class seed shall be hybrid seed to be planted for
           any use except seed production.
     *(Applicable if male sterile line is used)
II.    Land requirements

       A seed crop of hybrid marigold shall not be eligible for certification
       if planted on land on which the same kind of crop was grown in
       the previous year unless the crop (s) grown in the previous year
       was/were of the same variety and of an equivalent or higher class
       of certified seed and was/were certified.

III.   Field Inspection

       A minimum of four inspections shall be made as follows:

       1.    The first inspection shall be made at the state of 6-7 pairs of
             leaves in order to determine the isolation, volunteer plants,
             out crosses, planting ration (demarcation between female
             and male block if planted separately) and other relevant
             factors;

       2.    The second and third inspection shall be made during
             flowering to check isolation, pollen shedding heads in seed
             parent, off types and other relevant factors;

       3.    The 4th inspection shall be made at maturity and prior to
             harvesting to verify the designated diseases, true nature of
             plant and capitulum and relevant factors.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields of marigold hybrids shall be isolated from the
       contaminants shown in column 1 of the table below by the
       distances specified in columns 2 and 3 of the said table:

              Contaminants                   Minimum distance (meters)

                                             Foundation         Certified

                     1                             2                3

 Fields of other varieties including              600              300
 commercial   Including   commercial
hybrids of same cultivar

Fields of the same hybrid (code                  600            300
designation) not conforming to Varietal
purity requirements for Certification

Between blocks of the parental lines in           -               5
case seed parent and pollinator are
planted in separate blocks.



B.    Specific requirements

                 Factor                       Minimum permitted (%)*

                                              Foundation      Certified

                    1                             2               3

Off types in seed parent at and after           0.050           0.10
flowering

Off types in pollinator at flowering            0.050           .010

Pollen shedding heads in seed parent            0.050           0.10
at flowering

** Plants affected by designated                 0.10           0.20
diseases at final inspection

* Designated diseases shall be:

Leaf spot         (Alternaria tagetica shome & mustafee)

Flower bud rot            (A. alternata (fr.) Keissler & dianthi Stevans &
hall)

Collar rot        (Rhizoctonia solani Kuhn)
V.   Seed standards
               Factor                    Standard for each classes
                                        Foundation       Certified
                  1                          2              3
Pure seed (minimum)                       97.0%           97.0%

Inert matter (maximum)                     3.0%           3.0%

Other crops seeds (maximum)               10/Kg           20/Kg

Weed seeds (maximum)                       5/Kg           10/Kg

Germination (minimum)                      70%             70%

Moisture (maximum)                         9.0%           9.0%

For vapour-proof containers (maximum)      7.0%           7.0%
               Descriptors – Flowers & Ornamental Plants

      Plant height, plant spread, leaf shape, branching habit, flower
type, days required for flowering, number of flowers per plant or per
stem, colour and size of flowers, flowering duration fragrance, petal
texture, cut flower life, longevity of flower on the plant, length of flower,
stem stalk, flowers single/semi-double, double, flower yield,
common/commercial use, suitable for domestic/export market,
suitability for growing under open/cover conditions and reaction to
diseases and pests.

B.     Specific for different crops:
       a)    Perennials

       b)    Rose

       Bud length, foliage flossyness, colour retention, bloom center and
       reaction to cold.

ii)    Carnation:

       Calyx splitting, bud opening and shape of Corolla margins.

iii)   Chrysanthemum:

       Photo-sensitiveness,     colour   rentention    and     disc   florets
       hidden/visible.

iv)    Orchids:

       Habitat (epiphytic/terrestrial), lip shape and sepal colour.

v)     Gerbera:

       Hollowness of flower stalk and petal shape.

vi)    Anthurium:

       Spathe shape, spadix shape and flowering type/non-flowering type.

b)     Bulbous Plants:

i)     Gladiolus: Spike weight, internode length, floret arrangement
       number of florets remain fresh at a time, branching of spike,
       dormancy period and corm and cormel multiplication.
ii)    Narcissus and Daffodil: Shape of perianth cup or corona, leaf
       colour and flowers in umbel or solitary.

iii)   Tulip: Flower shape and leaf colour

iv)    Hyacinth: Leaf colour

v)     Iris: Beard present/absent, beard shape and rhizomatus/bulbous.

TUBEROUS BEGONIA:

       Petal shape, growth habit, flower shape and foliage colour.

       c)    Annuals:

i)     Marigold:
       Flower weight, stem colour and foliage colour present/absent

ii)    China Aster:

       Growth habit and stem colour.

iii)   Carnation:

       Calyx – splitting and petal shape

iv)    Antirrhinum:

       Flower shape and growth habit.
4.   PLANTATION CROPS
SEED CERTIFICATION STANDARDS FOR COCONUT

Land Requirement:

      Avoid using the same nursery area again the nursery should not
be in any diseased area particularly root (wilt) and ganoderma disease.

i)    Selection of seed garden:

      Garden should have a record of consistently high yield

Garden should contain a high proportion of heavy bearers

Garden should be free from the incidence of disease and not prone to
severe attack of pests

Do not select mother palms from very small holdings maintained under
highly favorable conditions of growth.
Palms located near cattle sheds, compost or manure pits, waterways etc.,
should preferably be avoided.

ii)   Selection of mother palms:

Palms selected as mother palms should be regular bearers and should
give annual yield of not less than 80 nuts under rain fed conditions.

The copra content per nut should be around 150 g.

Select palms which have reached full bearing stage and giving regularly
high yield for at least four consecutive years

Very old palms (above 60 years) should be avoided

Mother palm should have at least 30 fully opened leaves on the crown

The leaves should have short and strong petioles with vide leaf base
firmly attached to the stem.

The trees with crown having leaf orientation to all directions which are
commonly known as umbrella stage is ideally suited as mother palms.

Trees having a drooping or upright position for the leaves are generally
poor yields and such palms should be avoided.
Every leaf axil should have one inflorescence with a large number of
spikes
The female flower distribution per spike should be one or two which
insures high set and stability in yield,

At any time, there should be minimum 12 bunches with nuts at different
stages of development on the crown.

Peduncle should be short and stout so that there is no chance of bucking
of the bunches.

Palms producing barren nuts, alternate bearing irregular should be
avoided.

iii)   Selection of nuts:

For seed purpose; nuts of round and oblong shapes without ridges
should be selected.

Palms having higher proportion of husked nut, thickness or kernel and
size of the embryo should be selected.

The seed nut collection season should be adjusted according to the rainy
season of the area.

Tall cultivars, two months storage of seed nut is required for greater
germination

Nuts with fully dried husk alone should be sown lest germination will be
delayed.

15 days to 1 month storage is required for dwarf nuts
Store the nuts under shade, to retain nut water
Seednuts should be fully matured (i.e.,) 12 month old.

Nuts with irregular shape, size and improper development should be
rejected.

Nuts having cracks on the husk at the stalk or at the base should be
discarded.
SEED CERTIFICATION STANDARDS FOR COCOA

I.     Application for general seed certification standards.

(a)    The general seed certification standards are basic and along with
       the following standards it forms the standards for certification of
       cocoa plants for varieties pods.
(b)    The general standards are amplified as follows to apply specifically
       to cocoa. All clarified classes fo cocoa viz. Criollo, Forastero,
       trinitaria and hybrids shall be produced through graft/budded
       (clonal) plants whose source and identifications may be ensured
       and approved by certification agency.

II.    Land requirements

(b)    Nursery area should be away from old and ill managed cocoa
       gardens as well as away from cashew plots so as to avoid
       infestation of scion materials with ten-mosquitoes.
(c)    The area should be free of cocoa swollen shoot virus, vascular
       streak disease and phytophthora stem canker disease.

III.   Varieties

       (a) Selection of mother plants as per the specific characters of the
           variety has to be inspected and approved.
       (b) Mother scion plats must not be from a diseased tract and the
           plant must be free of disease.
       (c) Mother scion plants must be an heavy bearer, with precocity,
           high vigor, less pod index and with other quality parameters
           like high been size <(1.0 g) less shelling percentage (<10.12%)
           and high butter content (>55% and with tolerance to stresses of
           biotic and abiotic nature.
       (d) Nurseries inspection should be carried at 4-5 times to confirm
           the pedigree, and proper, maintenance, to cull out the diseased
           and off types and to ensure the proper clonal multiplications


IV.    Field standards

A.     General requirements

I.     Isolation

      The biclonal orchards with known compatible cocoa lines may be
maintained at an isolation distance of 300 mm from other blocks. So as
to avoid mixing of pollens in the desired hybrid combinations. Otherwise
it does not carry any meanings while only scion materials are to be
procured from the mother plants block. Polyclonal orchards must be
avoided in hybrid pod production.

II.   Specific

     Production of clones o f selected mother varieties can be carried
out through soft wood wedge grafting or patch budding using only
chupons (orthotropic branches with helical leaf arrangement and
symmetrical leaves) as scion sticks / budwoods.

The hybrid pods and the seedlings there of from the biclonal orchards
must be subjected to verification with reference to their F1 hybrid
characters as per the descriptors.

Characters of clones

      Six months old grafted or budded clonal cocoa plants with vigorous
scion having hardened green 5-6 leaves and no sprouts from rootstock
portion are ready for planting on to mainfield.

      The clones/hybrid seedlings must be free of pests and diseases.
SEED CERTIFICATION OF ARECANUT

I.           Application for general seed certification standards.

    3. The general seed certification standards are basic and along with
       following standards it forms the standards for certification of
       arecanut seeds for varieties.
    4. The general standards are amplified as follows to apply specifically
       to arecanut. All clarified classes of arecanut viz. Tall and semi tall
       shall be produced through seednuts which source and identify
       may be ensured and approved by certification agency.
II.    Land requirements

       (b)      Land to be used for nursery shall be free of old nuts and must
                be protected from nuts falling from arecanut palms if nursery
                area is in the arecanut garden. Swammy and clayey conditions
                may be avoided.
       (c)      Avoid using the same nursery area again. The nursery should
                not be in any diseased area particularly yellow leaf Disease.

III.         Varieties

       (a) Selection of mother palms as per the specific characters of the
           variety has to be inspected and approved.
       (b) Mother palm must be from a diseased tract. Palms must be free of
           disease.
       (c) Inspection during harvesting of seednuts must be carried out.
           Mother palms should be early bearers with high percent age of
           fruit set, and more than ten years old. From these palms, fully ripe
           and heavier (>35g) nuts should be selected.
       (d) Nursery inspection must be carried out four times to rogue out off
           types. Seedlings markets specific to the varieties must be listed for
           rouging off types.

IV.          field standards

V.           General requirements

I.           Isolation

The mother blocks of arecanut varieties shall be isolated from the other
contaminants at a distance of 500 m. All off types in the population must
be removed.
a.      Specific

      Production of pure nuts of selected varieties must be carried out by
inter use mating/sip meting. The population from which the nuts are
produced must be confirm to the varietal characters as per the
descriptions.

Characters of seedlings

(i)     One year old seedlings should confirm approximately the following
        criteria apart from being vigorous.

        Cultivars                 Girth (cm)             No. of leaves
        Tall                      20 cm                  5-6
        Semi tall                 15 cm                  5-6

Twelve to eighteen months old seedlings with five leaves and high
selection index (SI) should be used for transplanting to the main field. SI.
No. of leaves x 40 – height. Shorter seedlings with 5 to 6 leaves are
preferred for planting.

(II)    Seedlings should be absolutely free of insects and diseases.
(III)   In case of sale of seednuts, seednuts should have germination level
        of 90% and above.
SEED/PLANTING       MATERIAL      CERTIFICATION       STANDARDS       FOR
CASHEW

ESTABLISHMENT OF CASHEW SCION BANK:

Land : Soils with water logging and excessive salinity or alkalinity should
be avoided.

Scion banks for the recommended cashew varieties should be established
by adopting a closer spacing of 4 m x 4 m

Plants should be fertilized with the recommended quality of NPK (500 g
N, 125 g 1205 and 125 g K2O per plant) during monsoon season.
Tender shoots should be protected from tea mosquito & other sucking
pests by giving timely sprays of insecticides *Endosulfan/
Monocrotophos @ 1.5 ml/l)

During flowering season, the flower panicles should be cut off in order to
get more no. of scions.

If mature scion sticks are not utilized due to some reason, such shoots
should be headed back so that more no. of lateral shoots are produced.

The plants in the scion bank should be maintained by heading back the
top (detopping) to a height of 1.5 m from ground level.

The pruned trees should be sprayed with Bordeaux mixture (1%)

Selection of scion and pre curing:

Select non-flowered lateral shoots of current season growth (3-6 months
old)

The selected shoots should be 10-12 cm long, straight, uniformly round
with pencil thickness brown coloured. The top leaves (4-5) should be
dark green in colour indicating proper maturity.

Precure by clipping off leaf blades. After 10-15 days scion sticks can be
detached

The procured scion sticks should be separated from the mother tree
before they sprout.

The procured scion sticks should be separated early in the morning.

Variety name should be labeled
The scion sticks should be dipped in water wrapped in moist cloth and
placed in polythene bag and brought to nursery shed.



Raising of root stick seedlings:

1.    Collect the seed nuts during peak period of Harvest (February-
      April)
2.    Seed nuts are sun dried for 2-3 days
3.    Medium sized nuts (6-7g) should be selected
4.    The seeds should be perfect formation (well filled) and with the
      highest possible specific gravity.
5.    The seeds should be treated with carbaryl (5 g/kg of seed) before
      storing in gunny bags.

Preparation of grafts:

1.    Root stock seedlings of about 60 days old should be used
2.    On the selected rootstock seedling, two pairs of bottom leaves are
      retained and the other leaves removed using a sharp grafting knife.
3.    At a height of 15-20 cm from ground level a transverse cut is made
      and the terminal shoot is removed.
4.    A cleft of 6-7 cm deep is made in that middle if the decapitated
      stem by giving a longitudinal cut.
5.    The wedge of the scion is inserted into the cleft of the rootstock
6.    The graft joint is secured firmly with 2-5 cm vide 30 cm long
      polythene strip of 100 gauge thickness.
7.    A white H.D. polythene bag (15 cm 12-5 cm 100 gauge) is inserted
      on the grafted. Plant tied at the bottom.
8.    The grafted plants are left for 2 weeks to encourage sprouting
9.    After 2 weeks polythene caps are removal
10.   70-80% grafts sprout within 3-4 weeks.

Management of Cashew grafts:

1.    Cashew grafts should be watered daily during dry periods.
2.    During heavy rains, excess water in bags should be removed by
      pressing the sides.
3.    Grafts should be arranged in beds after spreading black HD.
      Polythene sheet of 100 gauge.
4.    During summer month, grafted plants should be protected from
      sun by providing partial shade.
5.    Bordeaux Mixture 1%) spray to be given at 10 days interval as a
      prophylactic Measures.
6.    Insecticides such as Ekalux/Monocrotophas 1.5 ml/l to be sprayed
      to control sucking pests.
7.    The shoots arising from leaf axis of root stock below the graft joint
      to be removed.
8.    Flower panicles to be removed.
9.    Polythene strip should be removed from graft joint after 4 months
      of grafting to avoid girdling of the graft joint.
10.   The bottom leaves on the root stock should be removed after 3
      months of grafting when the scion leaves turn from bronze colour
      to green.
11.   5-12 months old grafts are to be supplied.
5.   SPICES
      CROP STANDARD FOR BLACK PEPPER (Piper nigram L.)
                   PLANTING MATERIAL

In black pepper, the rooted cuttings constitute the planting material.
Hence the standards are for the production of quality rooted cuttings.

Phase of certificate

1. Field Inspection for selection of the field (for single season certified
nursery – conventional method – three nodded cuttings).

1.1. Selection of mother vines:

The material meant for multiplication should be selected only from an
uniformly established gardens free from diseases/inoculants of diseases
such as Phytophthora foot rot, Stunt and Phyllody diseases. Elite mother
vines are to be identified at such gardens with the following
characteristics.

a. Age of the elite mother vines should at least be not less than 7 years.
b. The vine should have given stable yield of at least 2 kg dry
   pepper/vine/year for 4 consecutive years.
c. Management practices followed for those vines during the last five
   years should be noted.
d. These selected vines should be monitored at least once in a year for a
   possible infection of the disease and continuously monitored for
   future use.

   Vines with above characters are identified, labeled and runner shoots
   from such lines only should be used for large scale multiplication. In
   any such selection, varietal purity should be maintained. Close
   examination is essential to avoid physical mixing of varieties. To avoid
   such mixing, support trees and vines should be examined carefully so
   that if more than one variety of pepper vines are growing on the same
   tree support, such vines should be rejected.

1.2 Field inspection team:

The field from which planting material are to be collected should be
inspected by a team consisting of a breeder, pathologist, entomologist
and nematologist besides an agronomist.

1.2.1. Minimum specific number of field inspection:

Two inspections are to be carried out; one during October-November for
identifying mother vines with the specifications of the crop field as
mentioned in para 1.1 and the other during January-February to inspect
field for the incidence of pests and diseases if any particularly to locate
Phytophthora foot rot and stunt/phyllody diseases. A certificate to the
effect the vines selected meet the standards para 1.1. It is mandatory for
any commercial nursery to obtain this certificate annually.

2.    Standards for planting material production methods

2.1   Nursery inspection – standards

The are in which nursery is to be established in a well drained preferably
in a slightly elevated place. This surface soil (0-15 cm depth) in this are
should be tested for the present/absence of disease causing
fungi/bacteria/nematodes particularly for the fungus Phytophthora
capsici, nematode viz., Radophollu similes and Meloidogyne incognita.
These tests should be carried out by the qualified personnel.

2.2 Method
Collect the runner shoots from the mother vines selected as per the
criteria in the para 1.1. Keep the vines to prevent them from striking
roots in the soil. Separate the runner shoots from the vines during
February-march. The middle one third portion of runner shoots are
preferred for planting. Avoid very tender and too hard portions for the
shoot. Cut the shoots into pieces of 2-3 nodes. Clip the leaves, if any
leaving a small portion of the petiole on the stem. Dip the lower cut end
(up to 2 cm) of the cuttings in 1000 ppm solution of 3 Indol Butyric Acid
(IBA) for 45 seconds will substantially increase root formation and
development (This solution can be prepared by dissolving 1 g of IBA in
one litre of water containing 3-5 g of sodium carbonate (Washing soda).
Adhere to the dipping period of 45 seconds as any deviation from this
may be injurious.

Treating the cuttings with Seradix B2 is equally effective. But IBA
treatment is cheaper and hence is recommended for large nurseries
where technical supervision. Seradix B2 can be conveniently used by the
farmers and small scale nurseries.

Plant the treated cuttings in polythene bags (20 x 15 cm size with 300
gauge thickness) filled with potting mixture. The potting mixture is
prepared by mixing two parts of fertile top soil, one part of river sand and
one part of well decomposed Farm Yard Manure of Compost. When
polythene bags are used sufficient number of holes about 20) may be
provided at the base to ensure good drainage. The cutting should be
planted at least on node deep in the soil.
In large commercial nurseries, where the soil contains more than 200
nematodes per gram of soil, the potting mixture should be fumigated
with methyl bromide @ 500g per tonne of soil to destroy the nematodes.
Planting of cuttings in such a potting mixture is to be taken up only after
21 days of fumigation. The cuttings after planting should be kept under
good shade. The cuttings are to be well protected from direct sun light.
Light and frequent watering in recommended in the nursery to maintain
a humid and cool atmosphere around the cuttings. Heavy watering which
makes the soil slushy and cause water logging is to be avoided.


2.3. Minimum specifications for the rooted cuttings of black pepper

     i.       The age of the rooted cutting should be 21/2 moths old from the
              date of planting in the polythene bags.
     ii.      A minimum of five leaves should be preset with vigorous growth
              without exhibiting any nutrient deficiency symptoms
     iii.     Profusely developed roots with the absence Phytophthora capsici
              spores and nematodes viz., Radopholus similis and Meloidogyne
              incognita on the cuttings as well as in the potting mixture
     iv.      Maintaining varietal purity.
     v.       Each such bag may contain a minimum of two such rooted
              cuttings

     Black pepper rooted cuttings fail to meet above requirements should
     not be considered as a quality planting material of black pepper.

3.          Multi-season Nurseries

This nurseries are based on the bamboo method of rapid multiplication.

3.1. Nursery inspection

The nurseries should be inspected by an expert team consisting of a
plant pathologist, nematologist, entomologist and an agronomist and
certify that the nursery plants are free from sources of inoculums
specifically for the fungi Phytophthora capsici, nematodes incognita and
Radophollus similis, viral disease symptoms Stund and Phyllody disease.
This expert should inspect the nursery at least once in six months and
issue certificate as the case may be.

3.2. Minimum specifications for the rooted cuttings of black pepper

The standards prescribed as in para 2.3 are applicable here.
      i.         The age of the rooted cutting should be 21/2 months old from
                 the date of planting in the polythene bags.
      ii.        A minimum of five leaves should be present with vigorous
                 growth without exhibiting any nutrient deficiency symptoms
      iii.       Profusely developed roots with the absence Phytophthora capsici
                 spores and nematodes viz., Radopholus similis and Meloidogyne
                 incognita.
                 Black pepper rooted cuttings fail to meet above requirements
                 should not be considered as a quality planting material of black
                 pepper.
      iv.        Maintaining varietal purity
      v.         Each such bag may contain a minimum of two such rooted
                 cuttings

II           Land requirement/Nursery requirement

      Nursery shed to be used for propagation of black pepper shall be of
convenient size e.g., 24 m x 6m. Roof should allow sufficient light to pass
through it 950-75% light).

Rooting medium made of forest soil, sand, compost (or powdered cattle
manure in the para 1.1.1.

             *      Poly bag 15 x 10cms size.

III          Field inspection / Nursery Inspection

             Minimum of two inspections shall be made as follows:

      1. The first inspection shall be made about 30-35 days after
         planting the cuttings in the poly bag to check the sprouting.
   2.    The second inspection shall be made about 75-90 days to verify
         cutting growth Phytophthora, mealy bugs and scales infestation.
IV    Field standards
A.    General Requirements
      1.     Isolation

       The cuttings of particular variety shall be isolated from
contaminants shown in column 1 of the table below by the distances
specified in columns 2 and 3 of the said table.

Contaminants                              Minimum distance (meter)
                                       Foundation           Certified
Blocks of other varieties                  1                   1
Blocks of the same
variety not conforming to              1                          1
varietal           purity
requirements          for
certification

    B.      Specific requirements

Factor                    Foundation                 Certified
1. Sprouting                    95%                              90%
2. Phytophthora/viral             0                               0
   diseases                       0                               0
3. Scale     insect/Mealy
   bugs

Note:

    2.      Original mother plant should be free from Phytophthora,
            stunted and phyllody disease infestation whose runner shoots
            shall be used for propagation and it should be certified by
            authorities.
    3.      Periodical fumigation of nursery site as well as rooting medium
            is required.

*        Poly bag 15 x 10 cm size.

V. Cutting standards

                 Factor                 Foundation           Certified
    1. Height                             20 cm               15 cm
    2. Minimum No. of leaves                6                   4
    3. Phytophthora foot rot/leaf           0                   0
       spot                                 0                   0
    4. Virus infection                      0                 0-1%
    5. Nematode infestation                 0                 0-1%
    6. Scale/Mite/Thrips/Mealy
       bugs

Note:

      *     Cutting shoot be healthy, vigorous free from pest and disease
and also true to type
*     Before transporting it should be sprayed with approved pesticides.
CARDAMOM (Elerraria cardamomum Maton)

1. Application   and   amplification    of   general   seed   certification
   standards:

   A.    The general seed certification standards are basic and together
         with the following specific standards constitute the standards
         for certification of cardamom.
   B.    The general standards are amplified as follows to apply
         specifically to cardamom.

All certified classes shall be produced by seedlings raised from field
collected open pollinated seeds, seedlings, from hybrid seeds produced in
clonal seed production gardens and vegetative, through suckers (slips).

II. Nursery requirement/land requirement.

The nurseries should be established as far as possible only in virgin soils
or in soil where cardamom/other related zingiberaceous crops were not
planted earlier.

Avoid soil infected with pythium, phytophthora, Rhizoctonia, nematodes
and soil borne insect pests.
The land after preparation can be subjected to soil solarisation.

III. Field inspection/Nursery inspection.

For selection of mother plants for collection of open pollinated seeds
minimum two visit is needed. The first inspection should be at time of
the full bearing stage in the previous season (August-September) for
marking the plants having high yield parameters. The second inspection
shall be made just before the harvesting season of the current year
(September) for assessing the performance of the selected plants and also
for pest and disease incidence.

For hybrid seeds from clonal crossing blocks minimum three inspection
shall be made i.e., before flowering during flowering to rogue off
unsuitable plants if any, and also to ascertain the freedom from pests
and diseases, third inspection shall be at harvest to assess the quality of
seed capsules.

In case of nursery, minimum here inspection shall be made first at 3
months after sowing and second at 6 months to check off-types, pest and
disease and seedlings shall be transferred to poly bags final inspection
made before at the time of distribution (9-10 months) to assess the
quality of seedlings and pest and disease incidence.
IV. Field standards
A. General requirements

1.        Isolation

The clonal crossing blocks of cardamom shall be isolated from the
contaminants.
Contaminants                Foundation          Certified
Fields/Blocks of general            200 m                      100 m

Note:
In nursery minimum 3 m isolation distance is required between varieties.

     A. Specific requirements

Standards                              Foundation      Certified
1. Uniformity of the crop              0               0
1. Present of diseases

     a.   Katte virus                  0               0
     b.   Clump rot                    0               0
     c.   Azhukal (capsule rot)        0               0
     d.   Kokke kandu                  0               0

2. present of pests

     a. Thrips                         0               0
     b. Capsule/shoot/Panicle          5%              10%
        borer                          0               0
     c. Root grube                     5%              10%
     d. White filed

V. Capsule/seedling standards

     A. Capsule Standard

Standards                         Foundation         Certified
2. Appearance                     Bold and healthy   Bold and healthy
3. Uniformity                     95%                85%
4. Thrips          affected       5%                 10%
   capsules                       5%                 10%
5. Borer affected capsules        5                  10%
6. Deformed capsules              1%                 5%
7. Contamination       with       Brownish black     Brownish black
   other
8. Colour of the seed      60%                    50%
9. Germination percent     80%                    70%
   a. without         acid
      treatment
   b. with acid treatment


B. Seedlings standard.

Standards                   Foundation            Certified
1. Height                   60-75 cms             60-75 cms
2. No. of leaves            5-7                   5-7
3. NO. of suckers           -                     -
4. presence of diseases
   a. Katte virus           0                     0
   b. Kokke kandu           0                     0
   c. Fungal diseases       0                     0
5. Insect pest              1%                    5%
6. Undesirable seedlings    5%                    10%

Note:

The seedlings are first sown in raised beds of convenient size at 6 months
seedlings are transferred to secondary nursery i.e., in poly bags of 25x10
size filled with nursery mixture.
CLOVE (Syzygium armaticum (L) Merrill & Perry)

1.       Application and amplification of general seed certification
         standards

         A.    The general seed certification standards are basic and
               together with the following specific standards constitute the
               standards for certification of clove.
         B.    The general standards are amplified as follows to apply
               specifically to clove
         No distinct varieties are recognized in India. The common method
         of propagation is through seeds collected from healthy and regular
         bearing trees.

II. Nursery requirements

        In order to select elite mother clove tree, minimum two inspection
         shall be made. One before flowering second at the time of flowering
        In the nursery, only one check at the time of distribution shall be
         made to assess the quality of seedlings

IV       Field standards

     A. General requirements
     1. Isolation
        AS there is no distinct varieties isolation is not required. However,
     in nursery clove seedlings kept 2 meter away from other crop
     seedlings to avoid pest infestation.

     B. Specific requirements

         Healthy and regular bearing mother clove tree with following
         characters:

          1. Number of terminals per          >10 First inspection
             branch                           >14 First inspection
          2. Number of clusters per branch    >100 Second inspection
          3. Number of flower branch          >1.5 cm Second inspection
          4. Length of flowers

V.       Seedlings standard

         Height                  -50 cm
Note:

       To reach sufficient height it will take 9-24 months depending upon
        management
       First, seed will be sown in sand beds, after germination seedlings
        will be transferred to poly bags
       Normally no pest and diseases infests clove seedlings in the
        nursery
CROP STANDARDS FOR CINNAMON (Cinnamomum verum Bercht. &
Prest.)

In cinnamon, the rooted cuttings constitute the planting material. Hence
the standards are for the production of quality rooted cuttings.

1.        Nursery requirements

     a)      A nursery shed which can allow 75% sunlight
     b)      IBA or IAA 2000 ppm or Seradise-B
     c)      Polythene bags of 30x22 cm size and 300gz thickness for
             rooting
     d)      Coir dust and sand mixture (1:1 ratio)
     e)      Potting mixture made up of well powdered garden/forest soil,
             pure fine sand and well powdered dried cow dung (3:3:1 ratio)

2.        Parent tree requirements:

      The parent trees must have a very good regeneration capacity, high
quality bark with good yield. IISR Navashree and IISR Nithyashree are
the national varieties released recently.

3.        Planting material (rooted cuttings) production:

       Six month old shoot cuttings with 2-3 nodes are to be made from
the present tree. Half leaf only has to be retained at each node. The lower
cut end of the cutting (up to 2 cm) has to be dipped in 2000 ppm
solution of IBA or IAA for few seconds (quick dip method). This will
substantially increase root formation and development. This solution can
be prepared by dissolving 1g of IBA or IAA in 2 l. of water containing 3 to
5 g of Sodium Carbonate (washing soda).

      Treating the cutting with Seradix B2 is equally effective. But IBA or
IAA treatment is cheaper and hence recommended for large nurseries
where technical supervision is feasible. Seradix B-2 can be conveniently
used by farmers and small scale nurserymen.

       The shoot cuttings which were kept in the polythene bags could be
taken 2 to 3 months later, when roots were found through the polythene
bag. The rooted cuttings must be carefully lifted and separately planted
in polythene bag containing 3:3:1 garden soil, sand and farm yard
manure (dried and well powdered). At the time of planting, care has to be
taken that primary root is well developed (minimum 25 cm) with lot of
lateral roots (about 20). One year old such bagged cuttings must be used
for planting. It should have attained a minimum height of 25 to 30 cm.
GINGER (Zingiber officinale Rose)

I.     Application    and   Amplification   of   General   seed   certification
       standards.



II.    The General seed certification standards are basic and together
       with the following specific standards constitute the standards for
       certification of seed ginger.

A.     The general standards are amplified as follows to apply specifically
       to ginger

All certified classes shall be produced by vegetative propagation of
underground rhizome, whose source and identity may be assured and
approved by the certification agency.

III.   Land requirements

           Partial shade with gentle sloppy landscape.

           Soil should be loose, friable and offer minimum resistance to
            rhizome development. Soil depth 30 cm or more, high organic
            matter and pH of 6-6.5 are favourable. Virgin forest soils
            particularly after defestation is ideal.

           Planting should be avoided if soil is infested with Pythium sp.,
            Pseudomonas solanacearum and Meloidogyne incognita.

           Land to be used for seed/planting material production of ginger
            shall be free of volunteer plants.

IV.    Field Inspection

       A Minimum of four inspections shall be made as follows:

       A.      The first inspection shall be made at the time of planting
               variety isolation, rhizome rot, seed piece weight and spacing.

       B.      The second inspection shall be made about 45-125 days
               after planting to check germination, sprouting, rhizome rot
               and shoot borer incidence.
         C.        The third inspection shall be made about 180-190 days after
                   planting to check off types, rhizome rot and Phyliosticta leaf
                   spot.

         D.        The fourth inspection shall be made before harvest of or
                   between 240-250 days after planting to verify rhizome rot,
                   scale insect and meal bug infestation.

V.       Field Standards

         A.        General Requirements

         Isolation

         The fields/blocks of seed ginger shall be isolated from the
         contaminants shown in column 1 of the table below by the
         distances specified in columns 2 and 3 of the said table.

                   Contaminants                       Minimum distance (meters)
                                                      Foundation            Certified
                         1                                 2                     3
Fields of other variety                                    3                     3
Fields        of   the   same   variety       not          3                     3
conforming    to    varietal           purity
requirements for Certification

B.       Specific requirement :

                   Factor                     Inspection       Foundation     Certified
                                                 stage                       (Maximum)
1. Spacing                                I                45x30 cm          45x30 cm
2. Seed piece weight                      I                20-25 g           20-25 g
3. Rhizome rot                            I                0                 0
                                          II to IV         0                 0
4. Shoot borer                            II to III        1%                5%
5. Off-types                              III              0.5%              1%
6. Phyllosticta  Leaf     spot III                         1%                5%
   Bacterial wilt (Rhizictonis
   solanecsyur)
7. Scale insect                           IV               1%                5%
8. Mealy bugs                      IV               1%             5%
Note:
1.    All off types and diseased plants should be rouged out along with
      rhizomes and destroyed.
2.    Gaps in the seed plot should not be more than 10.0%.

VI.     Seed standards

          Factor                  Foundation                   Certified
1. Appearance                  Healthy & plumpy           Healthy & plumpy
2. Uniformity                           95%                    85-90%
      (Minimum)
3. Dry rot (Maximum)                    1%                        5%
4. Phyllosticta                         5%                       10%
   (Maximum)
5. Scales (Maximum)                     1%                        5%
6. Mealy             bugs               1%                        5%
   (Maximum)

Note:

1.      In a seed lot, rhizomes not conforming to specific characteristics of
        a variety shall not exceed 0.5% and 1% (by number - maximum)
        for foundation and certified seed classes, respectively.

2.      The seed material shall be reasonably clean healthy and firm.

3.      Cut, bruised, or those damaged by insects shall not exceed more
        than 1.0% (by weight)
TURMERIC (Curcuma longa L.)

I.     Application     and   Amplification   of   General   seed   certification
       standards.

A.     The General seed certification standards are basic and together
       with the following specific standards constitute the standards for
       certification of seed turmeric.

B.     The general standards are amplified as follows to apply specifically
       to turmeric.

All certified classes shall be produced by vegetative propagation of
underground rhizome, whose source and identity may be assured and
approved by the certification agency.

II.    Land requirements

           Soil should be loose, arable and offer minimum resistance to
            rhizome development.

           Soil depth 30 cm or more, high organic matter of acidic soil.

           The crop of seed turmeric shall not be eligible for certification if
            grown on the land infested with Pythium sp., Pseudomonas
            solanacearum and Meloidogyne incognita.

III.   Field Inspection

       A Minimum of four inspections shall be made as follows:

       A.      The first inspection shall be made at the time of planting
               variety isolation, rhizome rot, seed piece weight and spacing.

       B.      The second inspection shall be made about 45-50 days after
               planting to check germination, sprouting, rhizome rot and
               shoot borer incidence.

       C.      The third inspection shall be made about 120-180 days after
               planting in order to verify off types, shoot borer and rhizome
               rot.

       D.      The fourth inspection shall be made before harvest of or
               between 240-250 days after planting to verify rhizome rot,
               scale insect and meal bug infestation.
IV.   Field Standards

      A.       General Requirements

      Isolation

      The fields/blocks of seed turmeric shall be isolated from the
      contaminants shown in column 1 of the table below by the
      distances specified in columns 2 and 3 of the said table.

             Contaminants                           Minimum distance (meters)
                                                 Foundation              Certified
                    1                                  2                      3
Fields of other variety                                3                      3
Fields of the same variety not                         3                      3
conforming    to    varietal   purity
requirements for Certification

B.    Specific requirement :

               Factor                 Inspection           Foundation      Certified
                                         stage                            (Maximum)
1. Spacing                                  I              45x30 cm        45x30 cm
2. Seed piece weight                        I               20-25 g           20-25 g
3. Rhizome rot                              I                  0                  0
4. Shoot borer                          II to III             1%                  5%
5. Off-types                               III               0.5%                 1%
6. Scale insect                            IV                 1%                  5%
7. Mealy bugs                              IV                 1%                  5%
Note:
1. All off types and diseased plants should be rouged out along with
   rhizomes and destroyed.
2. Gaps in the seed plot should not be more than 10.0%.

V.    Seed standards

           Factor                Foundation                           Certified
1. Appearance                  Healthy & plumpy                Healthy & plumpy
2. Uniformity                      95-100%                              85%
   (Minimum)
3. Dry rot (Maximum)                 1%                        5%
4. Scales (Maximum)                  1%                        5%
5. Mealy           bugs              1%                        5%
   (Maximum)

Note:

1. In a seed lot, rhizomes not conforming to specific characteristics of a
   variety shall not exceed 0.5% and 1% (by number - maximum) for
   foundation and certified seed classes, respectively.

2. The seed material shall be reasonably clean, healthy and firm.

3. Cut, bruised, or those damaged by insects shall not exceed more than
   1.0% (by weight).
C U M I N (Cuminum syminum)

Open pollinated varieties and Composites

I.     Application   and   Amplification   of    General   seed   certification
       standards.

       The General seed certification standards are basic and together
       with the following specific standards constitute the standards for
       certification  of   the     seeds   of   cumin     open-pollinated
       varieties/composites.

II.    Land requirements

       Land to be used for seed production of cumin should be free from
       volunteer plants and where cumin crop has not been taken over
       preceding two years.

III.   Field Inspection

       1. The first inspection shall be made before flowering preferably
          within 45 days of planting to determine isolation, volunteer
          plant off types and other relevant factors.

       2. The second inspection shall be made during 50% flowering to
          check the isolation, off types and other relevant factors.

       3. The third inspection shall be made at maturity and prior to
          harvesting to verify the true nature of plant and other relevant
          factors.

IV.    Field Standards

       B.    General Requirements

       Isolation

       Seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said Table.

            Contaminants                        Minimum distance (meters)
                                            Foundation             Certified
                     1                             2                    3
Fields of other variety                           800                 400
Fields of same variety not conforming          800             400
to varietal purity

B.    Specific requirement :

            Factor                       Maximum permitted (%)
                                    Foundation            Certified
Off type Plants                         0.10                 0.50
Objectionable weed plants*              0.05                 0.10
*Objectionable weed shall be plantago pumila

V.    Seed standards

                  Factor                    Standards for each class
                                        Foundati         Certified
                                           on
Pure seed (minimum)                      97.0%            97.0%
Insert matter (maximum)                  3.0%              3.0%
Other crop seed (maximum)                10/Kg            20/Kg
Weed seeds (maximum)                     10/Kg            20/Kg
Objectionable weed seeds (max)           5/Kg             10/Kg
Germination (minimum)                     65%              65%
Moisture (maximum)                        10%              10%
For vapour proof container (max)          8%                8%
CORIANDER (Coriandrum sativum)

Open pollinated varieties and composites.

I.     Application   and   Amplification   of    General   seed   certification
       standards.

       The General seed certification standards are basic and, together
       with the following specific standards constitute the standards for
       certification of the seeds of coriander open-pollinated varieties,
       composites.

II.    Land requirements

       Land to be used for seed production of coriander should be free of
       volunteer plants.

III.   Field Inspection

       A Minimum of three inspections shall be made as follows:

       1. The first inspection shall be made before flowering preferably
          within 45 days of planting to determine isolation, volunteer
          plants, off types and other relevant factors.

       2. The second inspection shall be made during 50% flowering to
          check the isolation, off types and other relevant factors.

       3. The third inspection shall be made at maturity and prior to
          harvesting to verify the true nature of plant and other relevant
          factors.

IV.    Field Standards

       A.    General Requirements

       Isolation

       Seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said Table.

            Contaminants                        Minimum distance (meters)
                                            Foundation             Certified
                     1                             2                    3
Fields of other variety                       200               100
Fields of the same variety         not        200               100
conforming to varietal purity
Off type plants                              0.1%              0.5%
*Objectionable weed plants                   None              None

B.    Specific requirement :

                  Factor                     Standards for each class
                                         Foundati         Certified
                                            on
Pure seed (minimum)                       97.0%            97.0%
Insert matter (maximum)                   3.0%              3.0%
Other crop seed (maximum)                 10/Kg            20/Kg
*Objectionable weed seeds                 None              None
Germination (minimum)                      65%              65%
Moisture (maximum)                         10%              10%
For vapour proof container (max)           8%                8%

*Objectionable weeds Lathyrus
FENNEL (Foaniculum vulgare)

Open pollinated varieties and composites.

I.     Application   and   Amplification   of    General   seed   certification
       standards.

       The General seed certification standards are basic and, together
       with the following specific standards constitute the standards for
       certification of the seeds of fennel open-pollinated varieties,
       composites.

II.    Land requirements

       Land to be used for seed production of fennel should be free of
       volunteer plants.

III.   Field Inspection

       A Minimum of three inspections shall be made as follows:

       1. The first inspection shall be made before flowering preferably
          within 45 days of planting to determine isolation, volunteer
          plants, off types and other relevant factors.

       2. The second inspection shall be made during 50% flowering to
          check the isolation, off types and other relevant factors.

       3. The third inspection shall be made at maturity and prior to
          harvesting to verify the true nature of plant and other relevant
          factors.

IV.    Field Standards

       A.    General Requirements

       Isolation

       Seed fields shall be isolated from the contaminants shown in
       column 1 of the Table below by the distances specified in columns
       2 and 3 of the said Table.

            Contaminants                        Minimum distance (meters)
                                            Foundation             Certified
                     1                             2                    3
Fields of other varieties                     200               100
Fields of the same variety         not        200               100
conforming to varietal purity

B.      Specific requirement :

                 Factor                      Maximum permitted (%)
                                         Foundati         Certified
                                            on
Off types                                  0.10             0.50
*Objectionable weed                       None              0.05

IV.     Seed Standards

                 Factor                      Standards for each class
                                         Foundati         Certified
                                            on
Pure seed (minimum)                       97.0%            97.0%
Insert matter (maximum)                   3.0%              3.0%
Other crop seed (maximum)                 10/Kg            20/Kg
Weed seeds (maximum)                      10/Kg            20/Kg
*Objectionable weed seeds                 5/Kg             10/Kg
Germination (minimum)                      65%              65%
Moisture (maximum)                         10%              10%
For vapour proof container (max)           8%                8%

     Objectionable weeds Dodder (Cuscuta spp.)
Suwa (Anethum suwa L.) or Indian Dill.

Open pollinated varieties and composites

I.     Application   and   Amplification   of    General   seed   certification
       standards.

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of the seeds of Suwa (Anethum raveolens L.) open-
       pollinated varieties, composites.

II.    Land requirements

       Land to be used for seed production of Suwa should be free of
       volunteer plants.

III.   Field Inspection

       A Minimum of three inspections shall be made as follows:

       1. The first inspection shall be made before flowering preferably
          within 45 days of planting to determine isolation, volunteer
          plants, off types and other relevant factors.

       2. The second inspection shall be made during 50% flowering to
          check the isolation, off types and other relevant factors.

       3. The third inspection shall be made at maturity and prior to
          harvesting to verify the true nature of plant and other relevant
          factors.

IV.    Field Standards

       A.    General Requirements

       Isolation

       Seed fields shall be isolated from the contaminants shown in
       column 1 of the table below by the distances specified in columns
       2 and 3 of the said Table.

            Contaminants                        Minimum distance (meters)
                                            Foundation             Certified
                     1                             2                    3
Fields of other varieties                   200               100
Fields of the same variety         not      200               100
conforming to varietal purity

B.    Specific requirement :

                Factor                     Maximum permitted (%)
                                         Foundation        Certified
Off types                                   0.10             0.50
*Objectionable weed                        None              0.05

IV.   Seed Standards

                Factor                     Standards for each class
                                         Foundation        Certified
Pure seed (minimum)                        97.0%             97.0%
Insert matter (maximum)                    3.0%              3.0%
Other crop seed (maximum)                  10/Kg             20/Kg
Weed seeds (maximum)                       10/Kg             20/Kg
Germination (minimum)                       65%              65%
Moisture (maximum)                          10%              10%
For vapour proof container (max)            8%                8%
Ajawain (Trachyspermum anmi L.)

I.       Application      and   Amplification   of    General   Seed   Certification
         Standards.

         The General Seed Certification Standards are basic and, together
         with the following specific standards constitute the standards for
         certification of the seeds of Ajawain (Trachyspeermum ammi L.)
         open-pollinated varieties, composites.

II.      Land requirements

         Land to be used for seed production of Ajawain should be free from
         volunteer plants.
III.     Field Inspection

         A Minimum of three inspections shall be made as follows:

         1. The first inspection shall be made before flowering preferably
            within 45 days of planting to determine isolation, volunteer
            plants, off types and other relevant factors.

         2. The second inspection shall be made during 50% flowering to
              check the isolation, off types and other relevant factors.

         3. The third inspection shall be made at maturity and prior to
            harvesting to verify the true nature of plant and other relevant
            factors.

IV.      Field Standards

         A.        General Requirements

         Isolation

         Seed fields shall be isolated from the contaminants shown in
         column 1 of the Table below by the distances specified in columns
         2 and 3 of the said Table.

                   Contaminants                      Minimum distance (meters)
                                                     Foundation          Certified
                         1                               2                   3
Fields of other varieties                               200                 100
Fields        of   the   same   variety   not           200                 100
conforming to varietal purity

B.    Specific requirement :

               Factor                Maximum permitted (%)
                                   Foundation        Certified
Off types                             0.10             0.50

II.   Seed Standards

               Factor                Standards for each class
                                   Foundation        Certified
Pure seed (minimum)                  97.0%             97.0%
Insert matter (maximum)              3.0%              3.0%
Other crop seed (maximum)            10/Kg             20/Kg
Weed seeds (maximum)                 10/Kg             20/Kg
Germination (minimum)                 65%              65%
Moisture (maximum)                    10%              10%
For vapour proof container (max)      8%                8%
FENUGREEK (Kasuri Methi (Trigonella foenumgracum L.) AND METHI
(Trigonella foenumgracum L.)



I.   Application and Amplification of General Seed Certification
Standards

       The General Seed Certification Standards are basic and, together
       with the following specific standards constitute the standards for
       certification of the seeds of Kasuri Methi and methi.

II.    Land requirements

       Land to be used for seed production of Kasuri Methi and methi
       shall be free of volunteer plants.

III.   Field Inspection

       A minimum of two inspections shall be made, the first before
       flowering, the second at the flowering and fruit stage.

IV.    Field Standards

       A.    General requirements

       1.    Isolation

       Seed fields offered for certification shall be isolated from the
       contaminants shown in column 1 of the Table below by the
       distances specified in columns 2 and 3 of the said table:

             Contaminants                  Minimum distance (meters)

                                           Foundation        Certified

                    1                           2                3

Fields of other variety                         50              25

Fields of the same variety not                  50              25
conforming     to     varietal  purity
requirements for Certification.
B.    Specific requirements

                Factor                     Minimum permitted (%)*

                                           Foundation     Certified

                   1                           2              3

Off types                                     0.10           0.20

** Objectionable weed plants                 0.010          0.020

* Maximum permitted at the final inspection.

** Objectionable weeds shall be:Senji (Melilotus spp.),

V.    Seed standards

                Factor                     Standard for each classes

                                           Foundation     Certified

                   1                           2              3

Pure seed (minimum)                          98.0%          98.0%

Inert matter (maximum)                       2.0%           2.0%

Other crops seeds (maximum)                  10/Kg          10/Kg

Total Weed seeds (maximum)                   10/Kg          10/Kg

* Objectionable weed seeds (maximum)         2/Kg           5/Kg

Other    distinguishable       varieties     10/Kg          20/Kg
(maximum)

Germination   including    hard   seeds       70%            70%
(minimum)

Moisture (maximum)                           8.0%           8.0%
For vapour-proof containers (maximum)       6.0%              6.0%

* Objectionable weeds are the same as given at IV.B. above.

								
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