DNA Candy Lab_ Eat Your Genes

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					Name: _________________________________________________________


                               DNA Candy Lab: Eat Your Genes
                                     Student Handout

Procedure
   1. Wash your hands and table.
   2. Get a paper plate/paper towel.
   3. Decide which DNA triplet you will make. The triplet or can be any
      combination of the bases Adenine (A), Guanine (G), Cytosine (C), or
      Thymine (T).
                  For example: AAA; Do not use this example- pick another
                     of the 64 codons- preferably one with 3 different
                     bases/nucleotides. Then use the base-pairing rules to
                     determine what the complementary bases would be in the
                     other half of the DNA molecule.

                                               Base-Pairing Rules

        Adenine bonds with Thymine                                     Guanine bonds with Cytosine

DNA Triplet ___________                                        Complementary Bases _________

     4. Determine how many of each color gummy bear you will need to make
        BOTH sides of your DNA molecule.

                                                Adenine = Yellow
                                                  Thymine = Red
                                                 Guanine = Green
                                                Cytosine = Orange


          Yellow ___                      Red ___                        Green ___                        Orange ___

          Get the gummy bear colors you need, 2 Twizzlers, and a few toothpicks.

     5. Assemble your DNA using ½ a toothpick to attach the gummy bears to the
        Twizzlers and a whole toothpick to connect the gummy bears on the left
        and right sides of your DNA.

          Example
                                          A                                                          T


Watson Side                               A                                                          T       Crick Side

                                          A                                                           T
DNA Candy Lab: Eat Your Genes
                                                                                                                             1
Modified by TJackson- Original had this citation, “Thanks to the originator of this lab whoever it may be. It is modified from
one presented at the beginning AP Biology teachers’ workshop at Agnes Scott during the summer of 1996.”
Name: _________________________________________________________


     6. After you get yours assembled, fill in the bases on the picture below.




DNA Replication

      When DNA replicates, it “unzips” and each side is used as a template to
make a new side of the DNA (following base-pairing rules).

     7. If you were to replicate this DNA molecule, what would each new DNA
        molecule look like. Fill in the picture below with the 2 new DNA molecules.

               Old DNA                New DNA                                             New DNA              Old DNA




                                             Protein Synthesis
Transcription
      Transcription is when DNA is used as the template to make
messengerRNA. Because this is an energy requiring process, you may eat a
RED gummy bear (you won’t need the red ones for the RNA).

     8. Using the “Watson” or left side of your DNA, transcribe it into a mRNA-
        make an RNA molecule following base-pairing rules. Fill in the picture. Get
        the appropriate gummy bear colors for the mRNA.

      Yellow ___                      Clear ___                      Green ___                       Orange ___

                                                Adenine = Yellow
                                                  Uracil = Clear
                                                 Guanine = Green
                                                Cytosine = Orange

     DNA                                                                                                 mRNA



DNA Candy Lab: Eat Your Genes
                                                                                                                             2
Modified by TJackson- Original had this citation, “Thanks to the originator of this lab whoever it may be. It is modified from
one presented at the beginning AP Biology teachers’ workshop at Agnes Scott during the summer of 1996.”
Name: _________________________________________________________


     Translation
        Translation is when the mRNA template is used to make a transferRNA
     molecule as the protein is being synthesized. Base-pairing rules still apply.

     9. Using the mRNA molecule as a template, translate this into a tRNA
        molecule. Get the appropriate gummy bears to make the tRNA. Fill in the
        picture when you are done.

      Yellow ___                           Clear ___                      Green ___                       Orange ___

                                             mRNA                        tRNA




     10. Use the chart in your textbook (BSCS page 237) to find the amino acid
         that is produced from the tRNA triplet or anticodon (mRNA triplets =
         codons, tRNA triplets = anticodons).

                                  Your amino acid is ______________.

Congratulations! You have successfully replicated DNA, and made a very tiny
protein. Please clean up your work area and answer the following questions.

Analysis Questions

     1. Use the following sequence to replicate DNA.
        ATT CGC GCA TCA
        ___ ____ ____ ____

     2. Again use the chart on page 237 to find the amino acids that correspond
        to the following anticodons.

          Anticodons: AUG                        UUU                  CAA                    AUU              UGA

          Protein:             ______            ________              _________             _______ ______

     3. DNA is found in the _____________ of the cell.

     4. A three-letter triplet that represents an amino acid is called a
        __________________.

     5. A three letter triplet that represents mRNA is called a ___________.

DNA Candy Lab: Eat Your Genes
                                                                                                                             3
Modified by TJackson- Original had this citation, “Thanks to the originator of this lab whoever it may be. It is modified from
one presented at the beginning AP Biology teachers’ workshop at Agnes Scott during the summer of 1996.”
Name: _________________________________________________________


     6. What organelle does mRNA go to out in the cytoplasm?

     7. Define transcription.


     8. Where does transcription occur?

     9. Define translation.


     10. Where does translation occur?

     11. How do amino acids get to the ribosomes?


     12. Who were Watson and Crick?


     13. Fill in the Venn Diagram to compare and contrast RNA and DNA.




DNA Candy Lab: Eat Your Genes
                                                                                                                             4
Modified by TJackson- Original had this citation, “Thanks to the originator of this lab whoever it may be. It is modified from
one presented at the beginning AP Biology teachers’ workshop at Agnes Scott during the summer of 1996.”

				
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