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					Applied and Environmental Microbiology, April 2008, p. 2288-                 This Article
2297, Vol. 74, No. 8
0099-2240/08/$08.00+0 doi:10.1128/AEM.02145-07                 Full Text
Copyright © 2008, American Society for Microbiology. All       Full Text (PDF)
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The Genomes of the Non-                                        74/8/2288 most recent

Clearing-Zone-Forming and                                      Alert me when this article is cited
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Natural-Rubber- Degrading
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Species Gordonia
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polyisoprenivorans and Gordonia                                Similar articles in PubMed

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Daniel Bröker, David Dietz, Matthias                                       Google Scholar
Arenskötter, and Alexander Steinbüchel*
                                                               Articles by Bröker, D.
                                                               Articles by Steinbüchel, A.
Institut für Molekulare Mikrobiologie und
Biotechnologie, Westfälische Wilhelms-Universität                              PubMed
Münster, Corrensstrasse 3, D-48149 Münster,
Germany                                                        PubMed Citation
                                                               Articles by Bröker, D.
                                                               Articles by Steinbüchel, A.
Received 19 September 2007/ Accepted 14 February
                                                               Pubmed/NCBI databases
2008
                                                                 Nucleotide                 Protein
The latex-clearing protein (LcpK30) from the rubber-       Substance via MeSH
degrading bacterium Streptomyces sp. strain K30 is
involved in the cleavage of poly(cis-1,4-isoprene),      Hazardous Substances DB
yielding isoprenoid aldehydes and ketones. Lcp             NATURAL RUBBER
homologues have so far been detected in all
                                                           POLYISOPRENE
investigated clearing-zone-forming rubber-degrading
bacteria. Internal degenerated oligonucleotides
                                                                         Agricola
derived from lcp genes of Streptomyces sp. strain
K30 (lcpK30), Streptomyces coelicolor strain A3(2),      Articles by Bröker, D.
and Nocardia farcinica strains IFM10152 and E1           Articles by Steinbüchel, A.
were applied in PCR to investigate whether lcp
homologues occur also in the non-clearing-zone-forming rubber-utilizing bacteria Gordonia
polyisoprenivorans strains VH2 and Y2K, Gordonia alkanivorans strain 44187, and Gordonia
westfalica strain Kb1, which grow adhesively on rubber. The 1,230- and 1,224-bp lcp-
homologous genes from G. polyisoprenivorans strain VH2 (lcpVH2) and G. westfalica strain Kb1
(lcpKb1) were obtained after screening genomic libraries by degenerated PCR amplification, and
their translational products exhibited 50 and 52% amino acid identity, respectively, to LcpK30.
Recombinant lcpVH2 and lcpKb1 harboring cells of the non-rubber-degrading Streptomyces
lividans strain TK23 were able to form clearing zones and aldehydes on latex overlay-agar plates,
thus indicating that lcpVH2 and lcpKb1 encode functionally active proteins. Analysis by gel
permeation chromatography demonstrated lower polymer concentrations and molecular weights
of the remaining polyisoprenoid molecules after incubation with these recombinant S. lividans
strains. Reverse transcription-PCR analysis demonstrated that lcpVH2 was transcribed in cells of
G. polyisoprenivorans strain VH2 cultivated in the presence of poly(cis-1,4-isoprene) but not in
the presence of sodium acetate. Anti-LcpK30 immunoglobulin Gs, which were raised in this study,
were rather specific for LcpK30 and did not cross-react with LcpVH2 and LcpKb1. A lcpVH2
disruption mutant was still able to grow with poly(cis-1,4-isoprene) as sole carbon source;
therefore, lcpVH2 seems not to be essential for rubber degradation in G. polyisoprenivorans.


* Corresponding author. Mailing address: Institut für Molekulare Mikrobiologie und
Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149
Münster, Germany. Phone: 49-251-8339821. Fax: 49-251-8338388. E-mail: steinbu@uni-
muenster.de

  Published ahead of print on 22 February 2008.

 Supplemental material for this article may be found at http://aem.asm.org/.


Applied and Environmental Microbiology, April 2008, p. 2288-2297, Vol. 74, No. 8
0099-2240/08/$08.00+0 doi:10.1128/AEM.02145-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.




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Copyright © 2008 by the American Society for Microbiology. All rights reserved.

				
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