INTEGRAL WORKSHOP ON LOTUS PLANT TRANSFORMATION AND IN VITRO TECHNOLOGIES IGB A.B.T. 2nd Floor, Room n°2 24–28 April 2006 QuickTime™ an d a TIFF (Uncompressed) decompressor are need ed to see this picture. Organizers: Ani Barbulova and Maurizio Chiurazzi Institute of Genetics and Biophysics A.B.T., (IGB-ABT) Naples, Italy Useful Information: Maurizio’s telephone numbers 081-6132433 Mobile: 3383197175 Institute of Genetics and Biophysics A. Buzzati Traverso Via P. Castellino 111, Napoli Subway Line n°1, Station Montedonzelli B&B accommodation “I FIORI DI NAPOLI” Via F. Girardi, 92 Tel. 081 19577083 Mobile: 3405432539 Abstract I hope we will be able to spread our expertise in this field to other groups of the network by presenting a general overview of the in vitro procedures currently used in Lotus japonicus with both lectures and practical work. We’ll also give the possibility to perform your own transformation experiment. During the lectures some practical information about Lotus specific tools such as collection of transforming vectors, best promoters and antibiotics resistance cassettes, Agro strains etc will be provided. We also appointed lectures of invited speakers that in some cases will give general scientific talks concerning the field of plant transformation and plant genome structure. I’m going to send you all the information concerning accommodation and map of the city that, although esteemed as “difficult”, I’m sure you’ll enjoy a lot. We are looking forward to have you here. See you soon Maurizio Monday 24 April 09:30 – 10.00 10.00 – 11.30 Welcome Address - General information Lecture: Maurizio Chiurazzi – (IGB-ABT) Molecular bases of Agrobacterium-mediated transformation. Analysis and optimization of the crucial parameters. Coffee Break Practical: Ani Barbulova, Maurizio Chiurazzi – (IGB-ABT). Description of the aspects of the practical program. Lunch Break Practical: A. tumefaciens infection step of the root material Callus propagation. M. loti infection in vitro procedure. 11.30 – 11.50 11.50 – 12.50 12.50 – 14.30 14.30 – 18.30 Tuesday, 25 April 9.30 – 11.00 Lecture: Ani Barbulova - (IGB-ABT) General aspects of plant regeneration, organogenesis and somatic embryogenesis. Assessment of the embryogenic potential and regeneration capacity in Lotus japonicus. Practical: Ani Barbulova - (IGB-ABT) Description of the practical step to be performed in the afternoon Visit of the IGB-ABT Lunch Break Practical: Shoot propagation and rooting. Direct shoot organogenesis procedure. A.rhizogenes infection procedure. 11.30 – 12.00 12.00 – 12.30 12.30 – 14.30 14.30 – 18.30 Wednesday 26 April 9.30 – 11.00 Lecture: Barbara Hohn - Friedrich Miescher Institute, Basel, Switzerland. Transfer and integration of T-DNA of Agrobacterium tumefaciens. 11.00 – 12.30 Practical: Massimo Magni - Leica Microsystems SpA Leica Hardware and Software in fluorescence Stereomicroscopy. Lunch Break Practical: Roots washing and transfer on fresh medium. GFP analysis of transformed tissues in composite plants. 12.30 – 14.30 14.30 – 18.30 Thursday 27 April 9.30 – 11.00 Lecture: Barbara Hohn - Friedrich Miescher Institute, Basel, Switzerland. The flexible plant genome: molecular biology and environmental influences. Practical: Troubleshooting – What to do!! Lunch Break Lecture: Thomas Hohn - Friedrich Miescher Institute, Basel, Switzerland. Plant viral gene silencing : attack and counterattack Free afternoon 11.00 – 12.00 12.30 – 14.00 14.30 – 16.00 Friday 28 April 9.00 – 10.30 Lecture: Fabio Apone - Arterra Bioscience Srl, Naples, Italy. Gatheway Technology and T-DNA vector design. Practical: Transfer of the co-cultivated roots on selective medium. List of participants: 10.30 – 12.30 Paloma Rueda Romero, Mike Guther, Alfredo Credali, Irmi Host, Catalina Stedel, Ombretta Montanari, Selim Omrane, Alessandra Rogato, Enrica D’Apuzzo, Concettina Cozzolino, Francesco Nacca, Gea Guerriero, Assunta Tortora.
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