"mpl proposal risk assessment"
MPL Proposal Risk Assessment Doc No: MPL Proposal Risk Assessment V2 Date: 2nd June 2008 Page: 1 of 4 MPL PROPOSAL RISK ASSESSMENT PLEASE COMPLETE THIS FORM TO ENABLE AN INITIAL ASSESSMENT OF THE SAFETY ISSUES ASSOCIATED WITH YOUR EXPERIMENT. YOU MAY BE ASKED TO A COMPLETE FURTHER ASSESSMENT IF YOU ARE AWARDED TIME AT THE MPL. Note 1: Please be aware that the Membrane Protein Laboratory and Diamond Light Source are only licensed for Containment Level 1, this means we cannot currently accept any projects involving human (ACDP List) or animal (SAPO List) pathogens of higher than Hazard Group 1 including most human tissues, genetically modified organisms assessed as a class higher than 1 (GM Risk Assessment) or any toxins or micro-organisms covered by Schedule 5 of the Anti-Terrorism Crime and Security Act 2001 (ATCSA 2001). To assess whether your project involves any of these categories please refer to the following websites: Schedule 5 to ATCSA 2001: http://security.homeoffice.gov.uk/newspublications/publication-search/anti-terrorism-crimesecurityact/ATCSA_2001_Schedule_5_Order.pdf?view=Binary ACDP List Biological Agents: http://www.hse.gov.uk/pubns/misc208.pdf Specified Animal Pathogens Order 1998 (SAPO 1998): http://www.defra.gov.uk/animalh/diseases/pathogens/classification.htm Note 2: Users are responsible for HSE / DEFRA / Home Office license cover for transport of samples to and from Diamond Light Source Ltd. Principal investigator’s name: Name of scientist proposing to visit the MPL: Title of proposal: 1) Do any of the samples you will bring to Diamond include any of the following : Pathogen from Hazard Group >1 (according to current lists of both ACDP and Specified Animal Pathogens Order 1998)* Yes No Genetically Modified Organisms Category >GM1 (as assessed by recognised GMSC according to ACGM guidance)* Yes No Micro-organisms or toxins covered by Schedule 5 (current version) of Anti-Terrorism, Crime and Security Act 2001* Yes No Human Tissue samples other than hair samples or nail clippings* Yes No *) Please note that if you have replied ‘Yes’ to any of the above questions we will not at this moment be able to accommodate your project at MPL / Diamond since we currently do not have the facilities to accommodate projects higher than Containment Level 1. Although in the future these arrangements may change, this would require significant modifications. It is therefore unlikely that a project of this type would be given time at the MPL in the first application rounds. MPL Proposal Risk Assessment Doc No: MPL Proposal Risk Assessment V2 Date: 2nd June 2008 Page: 2 of 4 2) Please provide details of the samples you propose to bring to the MPL: Plasmid or expression vector, as purified DNA Yes If yes, please provide details, including the intended cell lines you will transform with this vector: No Genetically modified organisms for expression of protein in the MPL Yes No If yes, please provide details of the cell line (including phenotype) and vector (including antibiotic resistance markers and other genes on the plasmid): Wild type organism for purification from the native source Yes No If yes, please provide details, including the name of the organism and any associated hazard categories: Cell pellets Yes No If yes, please provide details, including cell line, phenotype, vector and antibiotic markers. Please also include information on whether you have confirmed that there are no live cells present. If live cells could be present you will need to complete a GMO assessment: Pure protein Yes No If yes, please provide full details, including protein name, function, source organism and its hazard group, expression system, ligands, substrates, buffers or other reagents in the sample: Protein crystals If yes, please provide details: Yes No Other Yes No If yes, please provide details including the name of the organism and any associated hazard categories: 3) Sample storage: Do you have any specialist storage needs for your samples? If yes, please provide details: Yes No 4) Which other specific hazards are associated with your sample? Pathogenic to plants or environment Non-human tissue sample Viable organism Virulence factor / toxin Prion or part of prion Hazardous ligand Radioactivity* Flammable / Explosive Irritant / Harmful Dust Corrosive Reactive with air/oxygen/water/acid Toxic / very toxic Mutagenic Carcinogenic Teratogenic Sensitising / Allergenic Is designated a Work Exposure Limit (WEL)** Potential contamination Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No No No No No No No No No No No No No No No MPL Proposal Risk Assessment Doc No: MPL Proposal Risk Assessment V2 Date: 2nd June 2008 Page: 3 of 4 Other hazard Yes No If you have answered ‘YES’ to any of the above, please provide details for each relevant sample/substance including quantities: *Please provide details of isotopes, number of samples, total activity (Bq), sealed/unsealed form (solid, powder or liquid) and specify if samples contain uranium, thorium or plutonium. ** Any substance/material which has a WEL given in the current HSE Guidance Notes EH40 is a substance hazardous to health - please explain whether limit likely to be reached under normal/emergency conditions. 5) Any other hazards associated with experiment, equipment use or sample preparation at Diamond: Lasers Gases Robots (*) High Voltage / Electricity Pressurised vessels No BS / EN standards (e.g. Made-In-House) High Temperature New Personnel (please inform us of training needs) Other Yes Yes Yes Yes Yes Yes Yes Yes Yes (specify class below) No No No No No No No No No (*) The MPL has a crystallisation robot, which may be used for crystallisation experiments. This robot has an interlock system which prevents users from entering the robot enclosure during operation. The liquid handler robot has a door which prevents users accessing the system during experimental runs. User training is in place to ensure that all users are informed of the risks and trained to use the robot in a safe way. Please describe any additional hazards: 6) Which controls will you require to be in place: Special Transport (to / from Diamond, to be provided by User) Yes Special Transport (inside Diamond) Yes Secure Storage Yes Restricted Access Yes General Ventilation Yes Local Exhaust Ventilation Yes Biological Containment L2 / 3 Yes Microbiological Safety Cabinet Class I / II / III Yes Personal Protective Equipment (eg gloves, face mask, lab coat Yes Training Yes None of the above If you have answered ‘YES’ to any of the above, please provide details: No No No No No No No No No No 7) After your visit to the MPL Will you require your samples to remain at MPL after your visit? Yes No, samples will be removed If yes, please give details if you require any of your samples to be left stored in the crystallisation hotels or in the fridges or freezers in a clearly labelled box with the name contact details and MPL contact information: MPL Proposal Risk Assessment Doc No: MPL Proposal Risk Assessment V2 Date: 2nd June 2008 Page: 4 of 4 8) Approval Signature of Principal Investigator: Print Name: Date: Contact email and tel. number in case of queries: Signature of Principal Beamline Scientist/MPL group leader: Print Name: Date: