Citric Acid Production by Aspergillus niger Using Date Base Medium

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Citric Acid Production by Aspergillus niger Using Date Base Medium Fortified with Whey, Methanol and Tricalcium Phosphate
G. F. Mehyar1, K. S. S. Al-Delaimy2 and S. A. Ibrahim3 Univ. of Manitoba, Winnipeg, MB, CANADA, 2 University of Jordan, Amman, JORDAN, 3 North Carolina Agricultural and Technical State University, Greensboro, NC. Phone: (336) 334-7328 email:

Citric acid is widely used by the food, pharmaceutical, and chemical industries as a major substrate for the production of a variety of products. There have been increased interests in using natural resources such as fruit sugars for the production of citric acid. Research has shown that dates, figs, carob pods, kumara, taro and sugarcane are good substrates for citric acid production by Aspergillus niger. The objective of this study was to determine the ability of A. niger to produce citric acid from natural carbohydrate sources; date and sweet whey. The uses of other supplements to increase productivity were evaluated. Two strains of A. niger (ATCC 6275 and 9642) were grown in media containing different concentrations of date extract or molasses fortified with whey, methanol or tricalcium phosphate. The fermentation experiments were conducted at 25 ºC for 12 days and samples were withdrawn at different time intervals and analyzed for citric acid content. Results showed that high citric acid was produced by A. niger ATCC 6275 in 20 % molasses in whey with citric acid concentration of 32.4 g/L. When methanol and tricalcium phosphate were added, significant increase in citric acid production was recorded. Citric acid concentrations were 38.4 and 42.4 g/l, in media fortified with methanol and tricalcium phosphate respectively. Our result indicates that the addition of sweet whey, methanol and tricalcium phosphate date or molasses could have a significant influence on the production of citric acid by A. niger.

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