Analysis of estrogenic endocrine disruptors in food state-of-the

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LABERCA/BIOCOP/WP7 BIOCOP-CASCADE EDC Workshop Program v1.3 (03-11-2008) Analysis of estrogenic endocrine disruptors in food: state-of-the-art and future trends “From specific spectrometric approaches to emerging global transcriptomic approaches” BIOCOP (EU, 6th FP, IP) / CASCADE (EU, 6th FP, NoEx) Workshop Organised by School for Advanced Residue Analysis in Food (SARAF) _ 9th – 11th December 2008 Laboratoire d’Etude des Résidus et Contaminants dans les Aliments (LABERCA) Ecole Nationale Vétérinaire (ENVN), Nantes, France _ Page 1 of 3 LABERCA/BIOCOP/WP7 BIOCOP-CASCADE EDC Workshop Program v1.3 (03-11-2008) Program Tuesday 9th December 2008 13:30 – 13:45 Welcome Pf. Dr. Bruno Le Bizec (LABERCA, Nantes, France) Short presentation of SARAF, ENVN and LABERCA Description of the Workshop program Pf. Dr. Chris Elliott (Queen’s University, Belfast, Northern Ireland) Presentation of the BIOCOP project 13:45 – 14:00 Introduction 1. Introduction to Endocrine Disruption 14:00 – 15:00 1.1 The global Endocrine Disruption story Dr. Bernard Jegou (U625 INSERM, Rennes, France) Main historical dates and current evidences & focus on the “EDC in food” concern The specific Testicular Dysgenesis Syndrome story Pf. Dr. Niels Skakkebaek (Copenhagen University Hospital, Denmark) Main biological endpoints & relation with the “EDC” in food concern 15:00 – 16:00 1.2 16:00 – 16:15 16:15 – 17:15 Coffee break 1.3 QSAR: advantages and limitations. Alessandra Roncaglioni (Mario Negri Institute, Italy) Are these approaches predictive of the real activity? Round table and discussion: what priority targets? B. Le Bizec, J-P. Antignac, C. Elliott, B. Jegou, N.E. Skakkebaek, A Roncaglioni What main compounds, matrices, and specific vulnerable populations to survey? 17:15 – 18:00 1.4 18:00 – 18:15 18:15 – 18:45 19:30 Visit of the LABERCA Return to the hotel Dinner Wednesday 10th December 2008 2. Classical physico-chemical methods of analysis for EDC: the spectrometric approach 8:30 – 10:15 2.1 Basics of Mass spectrometry Pf. Dr. Bruno Le Bizec (LABERCA, Nantes, France) 10:15 – 10:30 10:30 – 11:15 Coffee break 2.2 Application of MS for measuring steroid estrogens in food and biological fluids Dr. Frédérique Courant (LABERCA, Nantes, France) Application of MS for measuring phytoestrogens in food Dr. Jean-Philippe Antignac (LABERCA, Nantes, France) 11:15 – 12:00 2.3 12:15 – 13:30 Lunch Page 2 of 3 LABERCA/BIOCOP/WP7 BIOCOP-CASCADE EDC Workshop Program v1.3 (03-11-2008) 3. Emerging biological methods of analysis for EDC: the transcriptomic approach 14:00 – 16:00 3.1 Basics of transcriptomic and Q-PCR techniques Pf. Dr. Hanspeter Naegeli (Zürich University, Zwitserland) 16:00 – 16:30 16:30 – 17:30 Coffee break 3.2 Basics of transcriptomic data processing and analysis Dr. Hans Gmünder (Genedata AG, Basel, Switzerland) 17:30 – 18:00 19:30 Dinner Return to the hotel and free time Thursday 11th December 2008 3. Emerging biological methods of analysis for EDC: the transcriptomic approach 8:30 – 9:15 3.3 Demo.: application of transcriptomic for measuring phytoestrogens in food Pf. Dr. Hanspeter Naegeli (Zürich University, Zwitserland) Demo.: application of transcriptomic for measuring estrogenic mycotoxins in food Dr. Priscilla Bowens (Zürich University, Zwitserland) 9:15 – 10:00 3.4 10:00 – 10:30 10:30 – 11:15 Coffee break 3.5 Demonstration: dedicated DNA-Chip for controlling EDC in food. The ultimate goal M. Thomas Ellinger (Clondiag GmbH, Jena, Germany) Round table and discussion: advantages and limits of MS vs ‘omics approaches? B. Le Bizec, J-P. Antignac, H. Naegeli, P. Bowens, H. Gmüder, T. Ellinger 11:15 – 11:45 3.6 11:45 – 12:15 12:30 – 14:00 14:00 Closing session Lunch Departure for airport/railway station Page 3 of 3

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