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PROTEOMICS PROCESS FLOW
1.1 – Section 1.1
Proteomics Process Flow Chart
Extra-Cellular Protein Sample/Culture Intra-Cellular Protein
Remove Cells Lyse Cells
AcroPrep™ Filter Plates
for Cell Separation/Removal Remove Debris
AcroPrep Filter Plates
Protein Mixture for Lysate Clearing
Concentrate Sample
Antibody
Purification
Enchant Albumin Depletion Kits
Albumin Depletion/
Ion Abundant Protein Removal
Affinity Exchange Enchant Multi-Protein
Affinity Separation Kits
Enchant™ lgG AcroPrep Filter Plates
Purification Kits with with Mustang® Q & S BioSepra Resins
Protein A or G Columns Membrane Pre-Fractionation
Desalting/Concentration Charge Size AcroPrep Filter Plates
with UF Membrane
Assays Arrays BioSepra Resins, Centrifugal Devices
AcroPrep Filter Plates with
AcroWell™ Filter Plates Vivid™ Gene Mustang Q & S Membranes Minimate™ Centramate™ TFF Systems
With BioTrace™ PVDF & Array Slides
NT Membrane BioSepra Resins
LC Separation Gel Separation Western Blotting
Collect Fractions Destaining + In-Gel Digestion Protein Detection
& Buffer Exchange Sequencing
AcroPrep Filter Plates AcroPrep Filter Plates FluoroTrans® FluoroTrans W
with GHP Membrane Membranes Membranes
with UF Membrane
Centrifugal Devices Nanosep® Centrifugal Devices BioTrace™ PVDF & NT
with GHP Membrane Membranes
Minimate TFF Systems
BioSepra Resins
Desalting/Rebuffer Desalting/ AcroPrep Filter Plates
Concentration Concentration with UF Membrane
Centrifugal Devices
Minimate TFF Systems
Assay MS-Analysis Crystallography
BioSepra Resins
Protein Detection
www.pall.com 1
PRODUCT SELECTION
1.2 – Section 1.2
Application Guide
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Chromatography Products Sample Preparation
Ultrogel® AcA, Trisacryl® GF Size Exclusion • • •
Chromatography Resins
Ceramic HyperD® Ion Exchange Chromatography Resins •
Trisacryl, HyperD, HyperCel™ Affinity Chromatography Resins • •
SDR HyperD Solvent-Detergent Removal Resins
MEP HyperCel Hydrophobic Charge Induction •
Chromatography Resins
Mustang® Ion Exchange Membrane Devices •
Omega™ Ultrafiltration Membrane for Size Exclusion • • • •
Multi-Well Filter Plates
AcroPrep™ Plates with Hydrophilic Filtration Membranes • • • •
AcroPrep Plates with Ion Exchange Membranes † •† † †
AcroPrep Plates with Prefilters
AcroPrep Plates with Ultrafiltration Membranes for Size Exclusion • • • •
AcroPrep Plates with Glass Fiber
AcroWell™ Plates with Binding Membranes
Centrifugal Devices/Spin Filters
Microfiltration (MF) Centrifugal Devices • • •†
Ultrafiltration (UF) Centrifugal Devices † •† • • •
†In combination with appropriate chromatography resin.
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1.2 – Section 1.2
PRODUCT SELECTION
3
PRODUCT SELECTION
1.2 – Section 1.2
Application Guide (continued)
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Protein Purification Kits Sample Preparation
Enchant™ Kit for Albumin Depletion •
Enchant Kits for IgG Purification • •
Enchant Multi-Protein Affinity Separation Kit • •
Tangential Flow Filtration Products
Minimate™ TFF Capsules • • • •
Ultrasette™ Lab TFF Devices • • • •
LV Centramate™ Lab TFF Systems • • • •
Centramate Lab TFF Systems • • • •
Prefiltration/Clarification
Acrodisc® Syringe Filters with Prefilters
VacuCap® Vacuum Filtration Devices
Detection Products
Vivid™ Gene Array Slides
FluoroTrans® PVDF Membranes
BioTrace™ PVDF Membrane
BioTrace NT Nitrocellulose Membrane
UltraBind™ Affinity Membrane
Immunodyne® ABC Membrane
Arrays
Protein Chip System Based on Arrays
Vivid Gene Array Slides
†In combination with appropriate chromatography resin.
4
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Sample Preparation
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1.2 – Section 1.2
PRODUCT SELECTION
5
PRODUCT SELECTION
1.2 – Section 1.2
Notes
6
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