Probe preparation by hcj

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									Supplementary Materials and methods

Specific probes for HFR1/SICS1, PIL1, PIL2, BIM1, HLH1, PHYA, IAA29, CKX5 were obtained by RT-PCR on total RNA extracted from seedlings exposed for 1h to low R/FR light using the following combinations of primers: HFR1/SICS1 forward 5’-GTCACAAGACGGACAAGGTTTCGGTTCTCG-3’ HFR1/SICS1 reverse 5’-CCAAACCGTGAAGAGACTGAGGAGAAGAAG-3’ PIL1 forward 5’-GTGTGTGAAAATGGGCAGATTCTTG-3’ PIL1 reverse 5-ATACAATTCCGTGGATCTACCAACAG-3’ PIL2 forward 5’-TTGTGTTTGAGAATGGCCAGA-3’ PIL2 reverse 5’-TCGATCCTTCACTGTCTTCAG-3’ BIM1 forward 5’-ACAAGAGAAAGCAGACAAGTATGTGACCT-3’ BIM1 reverse 5’-CTGTCCCGTCTTGAGCCGTTTT-3’ HLH1 forward 5’-CTCTCAGACTCATCATTCATCTCTC-3’ HLH1 reverse 5’-GTGATAATCTTCAACCTCCGAACTTC-3’ PHYA forward 5’-GCAGAAAGCCCTTGGATTTGGAG-3’ PHYA reverse 5’-CAGTGCTCAAACCCGTTGAATCC-3’ IAA29 forward 5’-GAGGGTGCTGCGTCTTGTTTGGG-3’ IAA29 reverse 5’-TTGTTCATCGCCGTGATCCTCCTAC-3’ CKX5 forward 5’-CTTGATTCTTGTTTCTTGAGTATTCTTTGA-3’ CKX5 reverse 5’-CAACCACCACACCGTTCCT-3’ The probe corresponding to GA-OX like was amplified from genomic DNA using the following primers: GA-OX like forward 5’-TCTTCTGGGTACCCAAAAGGTTG-3’ GA-OX like reverse 5’-GCAAAGCCCTGCAACAAATACAC-3’ The ELIP1 probe was obtained by restriction with Sal I and Not I of the 174P5T7 EST clone (ABRC, Ohio State University). The ATHB2 and ATL18 probes were previously described (Carabelli et al., 1993; Carabelli et al. 1996) Quantitative PCR experiments were performed using the following combinations of primers: FT forward 5’-AACGAACGGTGATGATGCCT-3’ FT reverse 5’-CATTTTCCTCCCCCTCTCAAT-3’

CO forward 5’-GATAAGACCGCGGGTCAATG-3’ CO reverse 5’-TGTTGAACCCTTGCTCCTCG-3’ ACT2 forward 5’-GACCAGCTCTTCCATCGAGAA-3’ ACT2 reverse 5’-CAAACGAGGGCTGGAACAAG-3’

References Carabelli, M., Sessa, G., Baima, S., Morelli, G., and Ruberti, I. 1993. The Arabidopsis Athb-2 and -4 genes are strongly induced by far-red-rich light. Plant J. 4: 469479. Carabelli, M., Morelli, G., Whitelam, G., and Ruberti, I. 1996. Twilight-zone and canopy shade induction of the Athb-2 homeobox gene in green plants. Proc. Natl. Acad. Sci. USA 93: 3530-3535.


								
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