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Table 3 Overview on purification data and ORF properties

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Table 3  Overview on purification data and ORF properties Powered By Docstoc
					Additional table 1: Overview on Gateway Entry Clones and results from automated protein expression screening No ORF ID
a

Accession No.

b

Gene ID

c

Gene Symbol

c

Protein Name

d

His

e

NusAe His

GST

e

MBP

e

MBPe His

MW f [kDa]

pI

g

Loc

h

25 30 37 25 30 37 25 30 37 25 30 37 25 30 37 1 DKFZp761A19121

AL136545

80036

TRPM3

2

DKFZp761A19121

AL136545

80036

TRPM3

3

DKFZp761I0112

AL136556

57223

SMEK2

4

DKFZp761I0112

AL136556

57223

SMEK2

5 6 7 8 9 10

DKFZp761I141 DKFZp761I141 DKFZp761G2023 DKFZp761J1923 DKFZp761J1923 DKFZp761K1524

AL136564 AL136564 AL136570 AL136574 AL136574 AL136581

83746 83746 26468 57447 57447 64430

L3MBTL2 L3MBTL2 LHX6 NDRG2 NDRG2 C14orf135

transient receptor potential cation channel transient receptor potential cation channel SMEK homolog 2, suppressor of mek1 (Dictyostelium) SMEK homolog 2, suppressor of mek1 (Dictyostelium) l(3)mbt-like 2 (Drosophila) l(3)mbt-like 2 (Drosophila) LIM homeobox 6 NDRG family member 2 NDRG family member 2 chromosome 14 open reading frame 135

1

-

-

2

1

-

-

-

-

-

-

-

-

-

-

36,8

10

cy,nu

1

-

-

3

1

-

-

-

-

-

-

-

1

-

-

36,8

10

cy,nu

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

32,3

5

ne

-

-

-

2

1

-

-

-

1

-

-

-

-

-

-

32,3

5

ne

1 2 -

1 1 1

-

1 1

2 2

-

-

-

-

-

-

-

-

1 1 1

1 1 1

69,3 69,3 40,0 40,8 40,8

6 6 8 4 4

nu nu nu go go

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

32,5

5

cy,nu

11 12

DKFZp761P2324 DKFZp761C222

AL136584 AL136587

65009 55326

NDRG4

13 14

DKFZp761G122 DKFZp564A2416

AL136591 AL110124

51440 26010

15

DKFZp564K1216

AL049933

2770

16

DKFZp564K1216

AL049933

2770

17 18 19 20

DKFZp564P1516 DKFZp564B2123 DKFZp564O0823 DKFZp564P1263

AL110128 AL136612 AL080121 AL136653

9374 83988 25849 11067

21

DKFZp564K0164

AL117618

5162

NDRG family member 4 AGPAT5 1-acylglycerol-3phosphate Oacyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) HPCAL4 hippocalcin like 4 LOC26010 viral DNA polymerasetransactivated protein 6 GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 PPT2 palmitoyl-protein thioesterase 2 NCALD neurocalcin delta DKFZP564O0823 DKFZP564O0823 protein C10orf10 chromosome 10 open reading frame 10 PDHB pyruvate dehydrogenase

-

-

-

3

2

-

-

-

1

-

-

-

-

1

-

37,1

5

go

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

42,1

9

mi

3

3

3

3

2

2

3

3

3

2

2

-

3

3

3

22,2

4

cy,nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

61,7

10

cs,mt-

-

-

-

2

-

-

2

1

2

-

-

-

-

2

-

40,3

5

go

-

-

-

3

2

-

-

-

-

-

-

-

-

-

-

40,3

5

go

3 -

3 -

3 -

3 -

2 -

-

3 -

3 -

3 -

1 -

1 -

-

3 -

1 3 -

2 -

34,9 22,2 25,0

6 5 4

er cy,nu go

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

23,4

11

uk

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

37,2

5

cy

22

DKFZp564N1272

AL136676

23710

GABARAPL1

23 24 25 26

DKFZp564N2378 DKFZp564J157 DKFZp564J157 DKFZp564E2182

AL136692 AL122042 AL122042 AL050261

29086 54458 54458 25842

HSPC142 PRR13 PRR13 ASF1A

27

DKFZp566J151

AL136702

3417

IDH1

28

DKFZp566J151

AL136702

3417

IDH1

29

DKFZp566E2324

AL136708

79992

C6orf59

30 31 32 33 34

DKFZp566N2024 DKFZp566N2024 DKFZp566N2024 DKFZp566B023 DKFZp566F123

AL136709 AL136709 AL136709 AL050273 AL050276

51225 51225 51225 25873 26137

ABI3 ABI3 ABI3 RPL36 ZBTB20

35

DKFZp566F123

AL050276

26137

ZBTB20

(lipoamide) beta GABA(A) receptorassociated protein like 1 HSPC142 protein proline rich 13 proline rich 13 ASF1 antisilencing function 1 homolog A (S. cerevisiae) isocitrate dehydrogenase 1 (NADP+), soluble isocitrate dehydrogenase 1 (NADP+), soluble chromosome 6 open reading frame 59 ABI gene family, member 3 ABI gene family, member 3 ABI gene family, member 3 ribosomal protein L36 zinc finger and BTB domain containing 20 zinc finger and BTB domain containing 20

3

3

3

3

2

-

3

3

3

2

-

-

2

3

3

14,0

9

cy,nu

-

-

-

2 3 3

3 3

2

-

1 1

1 1 1

2 2

2 1 2

-

1 1

2 2 3

1 2

36,6 15,4 15,4

4 10 10

cy,nu cy,nu cy,nu

3

3

3

2

-

-

3

2

3

2

2

-

2

3

3

23,0

4

nu

-

-

-

2

-

-

-

-

1

2

2

-

1

2

1

46,6

6

cy

-

-

-

1

-

-

-

-

1

2

2

-

1

2

2

46,6

6

cy

-

-

-

-

-

-

-

-

-

2

2

2

-

-

1

20,9

6

cy

-

-

-

3

2

2

-

2

2

-

-

-

-

-

2

38,9 38,9 38,9 12,3

4 4 4 12

cy cy cy nu,nl

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

73,4

6

nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

73,4

6

nu

36

DKFZp566G223

AL117424

25932

CLIC4

37 38 39 40 41 42

DKFZp566I133 DKFZp566I133 DKFZp566K013 DKFZp566K013 DKFZp566A0646 DKFZp566L1246

AL136711 AL136711 AL136712 AL136712 AL136715 AL136722

81671 81671 26052 26052 79719 30819

TMEM49 TMEM49 DNM3 DNM3 FLJ11506 KCNIP2

43

DKFZp434H0311

AL136742

649622

LOC649622

44 45 46

DKFZp434L0714 DKFZp434L0714 DKFZp434C2415

AL136752 AL136752 AL136757

60684 60684 79876

FLJ12716 FLJ12716 UBE1DC1

47

DKFZp434C2415

AL136757

79876

UBE1DC1

48 49 50

DKFZp434E1315 DKFZp434E1315 DKFZp434G1415

AL133049 AL133049 AL136759

25896 25896 83448

INTS7 INTS7 PUS7L

chloride intracellular channel 4 transmembrane protein 49 transmembrane protein 49 dynamin 3 dynamin 3 hypothetical protein FLJ11506 Kv channel interacting protein 2 similar to thioredoxin domain-containing 2 FLJ12716 protein FLJ12716 protein ubiquitin-activating enzyme E1domain containing 1 ubiquitin-activating enzyme E1domain containing 1 integrator complex subunit 7 integrator complex subunit 7 pseudouridylate synthase 7 homolog (S.

2

-

-

3

3

-

3

2

2

-

-

-

2

3

2

28,8

5

cy,nu

3

3

3

-

-

-

-

-

-

2

2

-

-

1

1

46,3 46,3 97,1 97,1 34,5

6 6 8 8 4

er er or or cy

3

3

3

3

3

-

3

3

3

1

1

-

3

3

2

26,0

4

cy,nu

-

1

-

3

3

-

3

1

3

3

3

2

2

3

1

12,7

4

cy,nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

91,8 91,8

6 6

cy cy

-

-

-

2

-

-

-

-

1

1

1

-

-

-

-

44,9

4

cy

-

-

-

2

-

-

-

-

1

1

1

-

-

-

-

44,9

4

cy

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

84,3 84,3

7 7

go go

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

80,7

7

nu

51

DKFZp434G1415

AL136759

83448

PUS7L

52 53 54

DKFZp434I0515 DKFZp434I0515 DKFZp434I1116

AL136761 AL136761 AL136769

81492 81492 56987

RSHL1 RSHL1 BBX

55

DKFZp434I1116

AL136769

56987

BBX

56 57

DKFZp434I0916 DKFZp434B0417

AL136772 AL133067

23213 29951

SULF1 PDZRN4

58

DKFZp434I2117

AL136777

83723

FAM57B

59 60

DKFZp434F1919 DKFZp434G131

AL136791 AL136795

60492 83449

CCDC90B PMFBP1

61

DKFZp434G131

AL136795

83449

PMFBP1

62

DKFZp434D0421

AL136804

81554

WBSCR16

63

DKFZp434D0421

AL136804

81554

WBSCR16

cerevisiae)-like pseudouridylate synthase 7 homolog (S. cerevisiae)-like radial spokeheadlike 1 radial spokeheadlike 1 bobby sox homolog (Drosophila) bobby sox homolog (Drosophila) sulfatase 1 PDZ domain containing RING finger 4 family with sequence similarity 57, member B coiled-coil domain containing 90B polyamine modulated factor 1 binding protein 1 polyamine modulated factor 1 binding protein 1 Williams-Beuren syndrome chromosome region 16 Williams-Beuren

-

-

-

3

-

-

-

-

-

-

-

-

-

1

-

80,7

7

nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

80,9 80,9

4 4

cy,nu cy,nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

101,6

8

uk

-

1 1

1 -

3

-

-

-

-

-

1 1

-

-

2 -

3 -

3 -

101,6 0,9 18,0

8 5 11

uk cy,nu nu,nl

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

25,2

8

er

-

-

-

2

-

-

-

-

-

-

-

-

1

-

-

29,5

7

mi

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

117,5

6

uk

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

117,5

6

uk

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

50,0

8

mi

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

50,0

8

mi

64 65 66

DKFZp434J1521 DKFZp434J1521 DKFZp434C2322

AL136805 AL136805 AL136809

57616 57616 11093

TSHZ3 TSHZ3 ADAMTS13

67

DKFZp434C2322

AL136809

11093

ADAMTS13

68 69 70 71

DKFZp434D0127 DKFZp434P2235 DKFZp434P2235 DKFZp434G099

AL136825 AL136860 AL136860 AL136904

84101 84218 84218 83452

USP44 TBC1D3 TBC1D3 RAB33B

72 73

DKFZp586G2219 DKFZp586E1222

AL136917 AL136930

7286 29097

TUFT1 CNIH4

74 75 76 77

DKFZp586E1323 DKFZp586B0424 DKFZp586B0424 DKFZp586J0624

AL136936 AL136940 AL136940 AL136944

26353 29062 29062 30061

HSPB8 HSPC049 HSPC049 SLC40A1

syndrome chromosome region 16 teashirt family zinc finger 3 teashirt family zinc finger 3 ADAM metallopeptidase with thrombospondin type 1 motif, 13 ADAM metallopeptidase with thrombospondin type 1 motif, 13 ubiquitin specific peptidase 44 TBC1 domain family, member 3 TBC1 domain family, member 3 RAB33B, member RAS oncogene family tuftelin 1 cornichon homolog 4 (Drosophila) heat shock 22kDa protein 8 HSPC049 protein HSPC049 protein solute carrier

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

98,5 98,5

8 8

mi mi

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

24,5

8

cy,nu

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

24,5

8

cy,nu

-

-

-

3 2 -

-

-

-

-

-

-

-

-

-

-

-

81,2 62,2 62,2

7 9 9

nu pm pm

2 -

3 -

-

2 2 -

-

-

2 -

2 -

2 -

-

-

-

1 -

2 -

1 -

25,7 44,3 10,6

7 5 4

go cy er

-

1 -

2 -

3 -

2 -

-

1 -

2 -

1 -

-

1 -

-

1 -

2 -

2 -

21,6 77,2 77,2 62,5

4 6 6 6

cy,nu cy cy pm

78

DKFZp586J0624

AL136944

30061

SLC40A1

79

DKFZp564H1122

AL136605

28970

C11orf54

80

DKFZp564H1322

AL136606

56006

C19orf61

81

DKFZp564I0422

AL136607

83591

THAP2

82

DKFZp564B1023

AL136611

83479

DDX59

83

DKFZp564I0123

AL136615

8575

PRKRA

84

DKFZp564A202

AL080056

4714

NDUFB8

85 86

DKFZp564D152 DKFZp564G182

AL136629 AL136632

7259 81688

TSPYL1 C6orf62

family 40 (ironregulated transporter), member 1 solute carrier family 40 (ironregulated transporter), member 1 chromosome 11 open reading frame 54 chromosome 19 open reading frame 61 THAP domain containing, apoptosis associated protein 2 DEAD (Asp-GluAla-Asp) box polypeptide 59 protein kinase, interferoninducible double stranded RNA dependent activator NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa TSPY-like 1 chromosome 6 open reading

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

62,5

6

pm

3

3

3

2

2

-

2

2

2

-

-

-

3

3

1

35,1

6

nu

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

57,7

7

cy,nu

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

26,3

10

nu,nl

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

64,6

6

nu-

-

-

-

3

2

-

-

-

-

2

2

-

-

2

1

34,4

8

cy

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

21,7

6

er

2 2

1 3

2 3

3 2

2 2

2 -

1 2

2 2

1 2

-

-

-

2 -

2 1

1 1

49,4 27,1

5 9

nu cy,nu

87

DKFZp564H012

AL136633

29074

MRPL18

88

DKFZp564M082

AL080071

25906

C11orf51

89

DKFZp564C1362

AL136647

10229

COQ7

90 91 92

DKFZp564A1164 DKFZp564K2478 DKFZp564N0582

AL136654 AL136690 AL050264

84063 11274 11170

KIRREL2 USP18 FAM107A

93

DKFZp566K144

AL136727

84084

RAB6C

94 95 96

DKFZp434P1735 DKFZp434J0450 DKFZp434E248

AL136859 AL136869 AL136897

55130 9400 55341

ARMC4 RECQL5 LSG1

frame 62 mitochondrial ribosomal protein L18 chromosome 11 open reading frame 51 coenzyme Q7 homolog, ubiquinone (yeast) kin of IRRE like 2 (Drosophila) ubiquitin specific peptidase 18 family with sequence similarity 107, member A RAB6C, member RAS oncogene family armadillo repeat containing 4 RecQ protein-like 5 large subunit GTPase 1 homolog (S. cerevisiae)

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

20,6

10

mi

-

-

-

-

-

-

2

-

2

-

-

-

-

-

-

14,3

3

cy,nu

-

-

-

3

-

-

-

-

-

2

-

-

-

1

1

20,3

5

uk

-

-

-

-

-

-

-

-

-

-

-

-

-

1 -

-

21,3 43,0

6 7

cy,nu nu

2

2

3

3

3

2

2

2

3

-

-

-

2

2

1

17,5

10

uk

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

23,3

6

go

-

-

-

2

-

-

3

3

3

-

-

-

2

2

2

0,6 19,3

3 10

mi nu

-

-

-

2

1

-

-

-

-

-

-

-

-

-

75,2

6

er

a

Additional information on the encoded proteins are accessible via http://www.LIFEdb.de Accession number according to GenBank entry c NCBI/Entrez gene symbol and gene ID d Protein name according to GenBank entry
b

e

Protein yield presented for the induction temperatures of 25°C, 30°C, 37°C. Numbering 1, 2, and 3 gives the apparent yield and concentration of each fusion protein. 1: 0.5 - 2.5 µg/mL; 2: 2.5 - 10 µg/mL; and 3: >10 µg/mL. Yield was estimated by comparison to BSA standards after Coomassie staining of SDS-gels. The preparation of entry clones failed for ORF no. 30, 31, 32, and the preparation of expression clones for ORF no. 20, 62 f MW refers to the size of respective proteins without tag. g pI is the isoelectric point of the protein without tag. h Subcellular localizations are abbreviated as follows: cy, nu: cytoplasm and nucleus; ne: nucleus-envelope; nu: nucleus; go: Golgi; mi: mitochondria; cs, mt: cytoskeletonmicrotubules; er: endoplasmatic reticulum; cy: cytoplasm; nu, nl: nucleus-nucleolus; pm: plasma membrane; or: others, uk: unknown


				
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