Hepatocellular carcinoma (HCC) is the most common form of

							Supplementary 1:


MTA1 promoter activity in HepG2 cells (A) or HEK 293 (B) transfected with either control vector

(250 ng/reaction) or HBx expression vector (250 ng/reaction) from different expression systems

(pcDNA 3.1, pSI-GFP, pSG5) was analyzed. Full-length murine MTA1 promoter (■)(-140 to -

5200) or its minimal promoter (-2872-5200)(□) were cloned into pGCL3 luciferase reporter vector

(Promega, Madison, WI).      Luciferase assay were performed according to the manufacturer's

instructions (Promega, Madison, WI), and the results were normalized against the -galactosidase

activity, an internal control. (C) Western blot analysis for transfection efficiency in HepG2 after

being cotransfected with pcDNA-p65 expression vector and HBx expression vector. Transfected

cells were analyzed for HBx, NF-κB-p65 48 hours after transfection. Vinculin was used as a

control. (D) NF-κB promoter activity in HepG2 cells transfected with either control vector or HBx

expression vector or HBx-deletion constructs (∆C-HBx-HBx with intact 101-120 amino acids and

∆E-HBx-HBx with intact 101-120 amino acids).



Supplementary 2:
qPCR analysis of MTA1 and HBx (WT and mutant) mRNAs in HepG2 cells transfected with either

control vector or HBx or mut-HBx expression vector. Expression levels of analyzed mRNAs were

normalized by β-Actin.




                                                                                                 1