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Non-Surgical Procedures and Techniques

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					Protocol # __________

NON-SURGICAL PROCEDURES AND TECHNIQUES
Describe each non-surgical procedure on a separate form. Complete each section below using the bulleted information as a guide. NAME OF PROCEDURE SPECIES Location of the procedure site (building/room #): 1. Describe the pre-procedure methodologies if applicable.  Acclimation period, fasting, baseline blood values, etc.

2. Describe the methods of restraint, sedation, or anesthesia.  Anesthetic agent, dose, volume and route of administration.  Method for determining surgical plane of anesthesia.  If paralytics are used, provide justification and planned anesthetic monitoring.

3. Procedure description detail-describe the following.  Experimental injections or inoculation-substances, doses, sites, volume, route, frequency, and duration.  Blood withdrawal-volume, frequency, withdrawal site, methodology.  Method of restraint-collars, vests, harnesses, metabolic cages.

4. Non-survival procedures If this procedure is non-survival, describe the method and timing of euthanasia and then skip to Qualifications for all personnel that will participate in this procedure.

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Protocol # __________

5. Immediate post-procedure monitoring (first 24 hours) -Describe immediate post-procedure care plans. Include the following:  What clinical parameters will be monitored and how often (such as TPR, temperature, pulse, and respiration)?  How will post procedure pain and distress be monitored (provide clinical signs)?  How will pain and distress be alleviated?  If the procedure involves more than momentary pain or distress not relieved by anesthesia, provide a scientifically based justification why analgesics cannot be used.

6. Long term post-procedure care plans  How will post-procedure pain and distress be monitored?  How will pain and distress be alleviated?  Justify the choice of analgesic.

7. Personnel for post procedure monitoring  Who will provide the post procedure monitoring and care? List the frequency and duration of monitoring.  What arrangements have been made for after hours monitoring and care if needed (include weekends and holidays)?

8. Adverse Effects  List the known or potential adverse effects of the procedure (e.g. drug toxicity, neurological abnormalities, etc).  Describe if mobility and accessibility to food and water, or the ability to groom, will be affected.  Describe how the adverse effects will be clinically managed?

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Protocol # __________ 9. Planned experimental effects and endpoints  Will any disease states be induced? If yes, describe.  State planned and/or experimental endpoints that will lead to euthanasia of the animal. Include: timepoints, tumor size, percent body weight gain/loss, behavioral or physical abnormalities, etc.

10. Multiple procedures: Describe the frequency with which animals will undergo the procedure and intervals between procedures. Include a justification for animals to be subjected to multiple procedures.

11. Qualifications for all personnel that will participate in this procedure. a. Which of the personnel named on page 1 of the main form will be performing the procedure? b. How were they trained in this procedure and in what species? c. If personnel are not trained for this procedure, describe how they will be trained?

12. Safeguards for personnel-describe if applicable.  Anesthetic gases-describe how exposure will be minimized (such as the use of scavengers.  Toxic chemicals-describe how exposure will be minimized (such as the use of a fume hood or downdraft table).  Other (list):

If assistance is needed in learning a procedure, please contact the veterinary staff in the DCM.

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Lingjuan Ma Lingjuan Ma
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