Solving Challenges in Flow Cytometry Flow Cytometry is one of the most powerful tools for quantitating the expression of individual cellular characteristics and demonstrating the unique effects of treatment on individual cells in a heterogeneous population. In this presentation, we will focus on how the flow cytometer instrument specifications can be understood and manipulated to result in the most flexible platform yielding the easiest to distinguish data. We will discuss simple fluorophore selections for multiplexing as well as covalent and affinity-based reagents (Zenon) for pre-labeling your primary antibodies. I will present data for the detection of multiple antigens simultaneously in the same detector by molar ratio variance using the Zenon technology. We will also discuss novel reagents for apoptosis detection, the most important of which allows for the multiplexing of two markers for apoptosis and a necrotic marker. I will also introduce a new easier to use alternative to JC-1. Lastly, we will discuss some underused applications in flow cytometry, including calcium indication.
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