P-Cell injury and cell death

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P-Cell injury and cell death Powered By Docstoc
					                                                                                 MBBS 1st Yr. Practical
                                                                                          Dr. U.S. Khoo
                                                                           October 23, 2002 - 10:45 AM

                         CELL INJURY AND CELL DEATH

G1   Kidney - coagulative necrosis (Infarct)

     A piece of normal kidney (N2) is included for comparison. Please note the
     morphology of normal glomerulus, renal tubules and blood vessels.

     A. Note:       -   Necrotic tissue: architecture preserved

                    -   Necrotic cells:
                         outline: shadowy
                         cytoplasm: eosinophilic
                         nuclei: faded & disappeared (karyolysis)

                    -   Compare with viable portion of kidney included in slide.

     B. Questions:      How would you describe the demarcation between the viable and
                        necrotic area? Is there any reaction?

G2   Lymph node: Caseous necrosis

     A section of normal lymph node (N1) is included for comparison. Lymp hoid follicles,
     paracortical zone and sinuses are present.

     A. Note:       -   part of lymph node preserved, but abnormal

                    -   necrotic area:
                          eosinophilic, amorphous, granular material
                          lacking cell outline. (Caseous = cheese- like)

     B. Question:       Can you find the differences between the necrotic lesions illustrated
                        in slide 1 and 2?

G3   Brain: Colliquative necrosis

     A section of normal brain (N5) is included for comparison. Glial cells are surrounded
     by fibrillary glial substance.

     A. Note:       -   loss of normal brain tissue
                    -   replaced by macrophages and cell debris
                    -   space created (fluid filled)

     B. Questions:      Why is this type of necrosis common in the brain?
                        What is the origin of the pigment inside some of the macrophages?

G5    Liver - Fatty Degeneration

      A section of normal liver (N3) is included. Preserved lobular architecture, portal tracts
      and central veins can be identified.

      A. Note:       -   Large intracytoplasmic space in liver cells - fat vacuole, with nuclei
                         pushed to one side

      B. Question:       What are the mechanisms that give rise to fatty change?

S56   Lymph node - Malignant lymphoma, Burkitt's type
      Apoptosis (Demonstration only)

      A. Note:       -   Loss of normal architecture and replacement of sheets of
                         monomorphic tumour cells.

                     -   Scattered cells showing shrinkage and condensation of cytoplasm
                         and nucleus. Such cells have eosinophilic cytoplasm and pyknotic

      B. Question:       What does frequent apoptotic bodies indicate in this tumour?