Shiu-Ru Lin Ph.D. CONTACT INFORMATION: Professor Faculty of Surgery, School of Medicine Kaohsiung Medical University 100 Shih-Chuan 1st Road, Kaohsiung, Taiwan Tel: +886-7-3114679 Fax: +886-7-3218864 E-mail: firstname.lastname@example.org EDUCATION: Kaohsiung Medical College, Ph.D., Medicine, 1989~1992 Kaohsiung Medical College, BS, Medical Techniology, 1981~1985 WORKING EXPERIENCES: 1985/08-1989/07 Assistant teacher at Department of Clinical Laboratory, Kaohsiung Medical College 1989/08-1992/07 Lecturer at Department of Clinical Laboratory, Kaohsiung Medical College 1992/08-1999/07 Associate professor at Department of Clinical Laboratory, Kaohsiung Medical College 1999/08Professor at Department of Clinical Laboratory, Kaohsiung Medical University RESEARCH INTERESTS: Our current research interests are focusing on eight major areas: 1) develop mRNA molecular markers for the detection of circulating tumor cells in various human cancers; 2) genotyping of chronic disease associated genes; 3) development of oligonucleotide chip and antibody chip for the diagnosis of colorectal cancer; 4) Identification of novel genes involved in the carcinogenesis of various human cancers; 5) development of SNP chip and Chemo-Chip for personalized chemotherapy; 6) integrated ologonucleotide chip for the early detection of human cancers; 7) functional genomics analysis of human cancers; 8) Noninvasive imaging of gene expression in living animals In addition to our own independent research, we are also actively involving in collaborative research projects. One is to cooperate with one Biotech Company at United States to develop type II-diabetic natural medicine by dual approach of functional genomic research and drug discovery. The other one is to collaborate with one Biotech Company at Canada to develop healthy food from compound by functional genomic research. PUBLICATIONS (within 5 years) 1. Wang JY, Hsieh JS, Chen CC, Tzou WS, Cheng TL, Chen FM, Huang TJ, Huang YS, Huang SY, Yang T, Lin SR. Alterations of the APC, c-met, and p53 Genes in Tumor Tissue and Serum of Patients with Gastric Cancers. J Surg Res (Accepted 2. in 2003). NSC91-2314-B-037-317. SCI. Chen FM,Hou MF,Wang JY,Chen TC, Chen David CP, Huang SU, Chung YS, Lin SR.2003 High frequency of G/C transversion on p53 gene alterations in breast cancers from Taiwan. Cancer letters (in press)(correspond)(SCI) 3. 4. Lai CL, Tai CT, Lin SR, Lin RT, Yang YH, Liu CK. 2003. Apolipoprotein E in Taiwan Chinese Patients with Dementia. Dementia 16: 208-211. Chen YF, Chiu HH, Wu CH, Wang JY, Chen FM, Tzou WH, Shin SJ, Lin SR. Retinoblastoma protein (pRB) was significantly phosphorylated through Ras-to –MAPK pathway in mutant K-ras stably transfected human adrenocortical cells. DNA Cell Biol (Accepted in 2003) (correspond). 5. Wang JY, Hsieh JS, Chen FM, Yeh CS, Huang TJ, Lin SR. High frequency of Activated K-ras Codon 15 Mutants in colorectal carcinomas from Taiwanese patients. Int J Cancer 2003;107:387-393. SCI. Hsieh JS, Wang JY, Lin SR, Lian ST, Chen FM, Hsieh MC, Huang TJ. Overexpression of nitric oxide synthase II in gastric mucosa of rats with portal hypertension: Correlation with gastric mucosal damage. J Surg Res 2003;115:24-32. NSC89-2314-B-037-067. SCI. 7. Wang JY, Chang CT, Hsieh JS, Chen YF, Lee LW, Huang TJ, Chai CY, Lin SR. Role of MUC1 and MUC5AC expressions as prognostic indicators in gastric carcinomas. J Surg Oncol 2003; 83: 253-260. NSC89-2314-B-037-131. SCI. Wang JY, Huang TJ, Chen FM, Hsieh MC, Lin SR, Hsieh JS. Mutation analysis of the putative tumor suppressor gene PTEN/MMAC1 in advanced gastric carcinomas. Virchows Arch 2003;442:437-443. NSC89-2314-B-037-120. SCI. Hsieh JS, Wang JY, Lin SR, Hsieh TJ, Huang TJ. Expression of Transforming Growth Factor α and Epidermal Growth Factor Receptor mRNA in the Gastric Mucosa of Portal Hypertensive Rats. 6. 8. 9. 10. Hepato-Gastroenterology. 2003;50:1305-1310. NSC-88-2314-B-037-073. SCI. 11. Ming-Chia Hsieh, Lin SR, Yuan-Chien Yang, Hung-Chun Chen,Jiun-Nong Lin and Shyi-Jang Shin.2002. Higher frequency of apolipoprotein E2 allele in type 2 diabetic patients with nephropathy in Taiwan. J Nephrol 2002;15:368-373. 12. Wu CH, Chen YF, Shin SJ, Lin SR. 2002 Mutant K-ras Oncogene Regulates Steroidogenesis of Normal Human Adrenocortical Cells by the RAF-MEK-MAPK Pathway..BJC 87: 1000-1005 (correspond). 13. Hsieh MC, Lin SR, Yang YC, Chen HC, Lin JN, Shin SJ.2002. Increased frequency of apolipoprotein E2 allele in type 2 diabetic patients with nephropathy in Taiwan Journal of Nephrology 7: 277-280. 14. Chong IW, Lin SR, Hwang JJ, Huang MS, Wang TH, Hung JY , Paulauskis JD. 2002.Expression and regulation of macrophage inflammatory protein - 1α gene by nicotine in rat alveolar macrophages. ECN 13(2); 242-9. 15. Wang JY, Liana ST, Chena YF, Yanga YC,ChenbLT, Lee KT, Tsung J, Huang TJ, Lin SR.2002.Unique K-Ras Mutational Pattern In Pancreatic Adenocarcinoma From Taiwanese Patients.Cancer letters 180:153-158(correspond). 16. Wu CH, Lee SC, Chiu HH, Yang YC, Lian ST, Shin SY, Lin SR. 2002, Morphological change and elevation of cortisol secretion in cultured human normal adrenocortical cells caused by mutant p21K-ras protein. DNA Cell Biol 21(1) 21-29 (correspond). 17. Yu FJ, Wu DC, Kuo CH, Lu CY, Su YC, Lee YC, Lin SR, Liu CS, Jan CM, Wang WM, 2001, Diagnosis of Helicobacter pylori infection by stool antigen test in southern Taiwan, Kaohsiung J Med Sci 17(7):344-50. 18. Wu DC, Kuo CH, Lu CY, Su YC, Yu FJ, Lee YC, Lin SR, Liu CS, Jan CM, Wang WM,2001, Evaluation of an Office-based Urine Test for Detecting Helicobacter Pylori: A Prospective Pilot Study. Hepato-Gastroenterology 48:614-617 19. Huang TJ, Wang JY, Lin SR, Lian ST, Hsieh JS, 2001, Overexpression of the c-met Protooncogene in Human Gastric Carcinoma--Correlation to Clinical Features. Acta Oncologica 40: 638-643. 20. Hsu CH, Lee SC, Yang YC, Lian ST, Shin SJ* and Lin SR, 2001, The p53 codon 249 mutant - derived from human functional adrenal tumors - can modify the cell shape of normal adrenocortical transfected cells. Cancer Letters 170(1) 63-71(correspond). 21. Hsu CH, Yang YC, Lian ST, Lee SC, Shin SJ, and Lin SR, 2001,. Significantly increased cortisol secretion in normal adrenocortical cells transfected with K-ras mutants deriving from human functional adrenocortical tumors. DNA & Cell Biol 20(4): 231-238(correspond). 22. Hsieh JS, Wang JY, Lin SR, Hsieh TJ, Huang TJ. Expression of Transforming Growth Factor α and Epidermal Growth Factor Receptor mRNA in the Gastric Mucosa of Portal Hypertensive Rats. Hepatogastroenterology 2001 (In Press). 23. Wang JY, Lin SR, Hsieh JS, Hsu CH, Huang YS and Huang TJ, 2001, Mutations of p53 gene in gastric carcinoma in Taiwan. Anticancer Res 21: 513- 520. 24. Hsieh MC, Lin SR, Hsieh TJ, Hsu CH, Chen HC, Shin SJ, Tsai JH, 2001, Increased frequency of angiotensin-converting enzyme DD genotype. Nephrology Dialysis Transplantation.15(7):1008-13. 25. Wang JY, Hsieh JS, Lin SR , Huang TJ, 2001, The effect of portal hypertension on expression of gastric mucin mRNA in rats. Hepato-Gastroenterology.48:667-671. 26. Hsieh MC, Lin SR, Hsieh JS, Hsu CH, Chen HC, Shin SJ, Tsai JH, 2000, Increased frequency of angiotensin-converting enzyme DD genotype in patients with type 2 diabetes in Taiwan, Nephrology Dialysis Transplantation 15(7):1008-13. 27. Hsieh TJ, Lin SR, Lee YJ, Shin SJ, Lai YH, Hsu CH, Tsai JH. 2000, Increased renal medullally endothelin-1 synthesis in pre-hypertensive DOCA and salt treated rats. The American Journal of Physiology 279:F112-F121. 28. Chong IW, Lin SR, Hwang JJ, Huang MS, Wang TH, Tsai MS, Hou JJ, and Paulauskis JD, 2000, Expression and regulation of macrophage inflammatory protein-2 gene by vanadiumin mouse macrophages. Inflammation 24(2):127-39. 29. Lai FJ, Shin SJ, Lee YJ, Lin SR, Jou WY and Tsai JH, 2000, Up-regulation of adrenal cortical and medullary atrial natriuretic peptide and gene expression in rates with deoxycorticosterone acetate-salt treatment, Endocrinol 141(1):325-332. 30. Lin SR, Hsu CH, Tsai JH, Wang JY, Hsieh TJ and Wu CH, 2000, Decreased GTPase activity of K-ras mutants deriving from adrenocortical tumors. Br J Cancer 82:1035-1040(correspond). 31. Chen BH, Chiang CH, Lin SR, Chao MG, Tsai S-T, 1999, The influence of Age at Onset and Gender on the HLA-DQA1, DQB1 association in Chinese Chidren with inculin dependent diabetes mellitus. Human Immunology. 60:1131-1137. 32. Shin SJ, Lai FJ, Wen JD, Lin SR, Hsieh MC, Hsiao PJ, Tsai JH, 1999, Increased nitric oxide synthase mRNA expression in the medulla of water-deprived rats. Kidney International. 56:2191-2202. 33. Shih MH, Sheu MM, Chen HY, Lin SR, 1999, Fungal keratitis caused by Candida utilis-case report. Kaohsiung J Med Sci 15: 171-174. 34. Lin CP, Tsai MC, Sun CY, Chen JY, Lin SR, 1999, Stability of self-prepared fortified antibiotic eyedrops. Kaohsiung J Med Sci 15: 80-86. 35. Wu DC, Wang WM, Lu CY, SU YC, Lin SR, Liu CS, Jan CM, 1999, The hemodynamic changes of gastroduodenal regional blood flow after Helicobacter pylori eradication in patients with duodenal ulcer scar. Kaohsiung J Med Sci 15:19-25. 36. Hsu CH, Yang SA, Yu SY, Lin SR, 1999, Mutational spectrum of p53 gene in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan. BJC 80(7):1080-1086(correspond). 37. Lin SR, Yang YC, Tsai JH, Hsu CH, 1998, Alteration of RET oncogene in human adrenal tumors. JPN J Cancer Res 89(6): 634-640. 38. Lin SR, Tsai JH, Yang YC, Lee SC,1998,Mutations of K-ras oncogene in human adrenal tumors in Taiwan, BJC 77(7): 1060-1065(correspond). 39. Chen BH, Lin SR, Chiang CH and Chao MC, 1998, Molecular characterization of Chinese G6PD deficiency by using polymerase chain reaction/single strand conformation polymorphism. Kaohsiung J Med Sci 14:197-202. 40. Chen KH, Sheu MM, Lin SR, 1997, Rapid identification of Mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis-a case report of corneal ulcer. Kaohsiung J Med Sci 13:583-588. 41. Chong IW, Lin SR, Lin MS, Huang MS, Tsai MS, Hwang JJ, 1997, Heparin-binding epidermal growth factor and transforming growth factor-a in human non-small cell lung cancers. J Formos Med Assoc 96(8): 579-85. 42. Lin SR,Yang YC,Chang CH,Tsai JH,1996,A significant decrease on the transcriptional activity of p53 mutants deriving from human functional adrenal tumors, DNA Cell Biol 15 (10):793-803(correspond). 43. Jan CM, Wu DC, Su YC, Wang WM, Liu CS, Lin SR, Chen CY, 1995, Evaluation of the severity of Helicobacter pylori infection with urease test: its corrrlation with histopathology and bacterial density, Kaohsiung J Med Sci 11(12):650-3. 44. Shin SJ,Lee YJ,Lin SR,Tan MS,Lai YH,Tsai JH,1995,Decrease of renal endothelin-1 content and gene expression in diabetic rats with moderate hyperglycemia, Nephron 70:486-493. 45. Lee YJ,Shin SJ,Lin SR,Tan MS,Tsai JH,1995,Increased expression of heparin binding-epidermal growth factor like growth factor mRNA in the kidney of strepto-zotocin induced diabetic rats. Biochem Biophys Res Commun 207:216-222. 46. Lee YJ,Lin SR,Shin SJ,Lai YH,Lin YT,Tsai JH,1994,Brain natriuretic peptide is synthesized in the human adrenal medulla and its messenger ribonucleic acid expression along with that of atrial natriuretic peptide are enhanced in patients with primary aldosteronism.J Clin Endocrinol Metab 79(5):1476-1482. 47. Wang WM,Chen CY,Jan CM,Chen LT,Peng DS,Lin SR,Lin CS,1994,Long-term follow-up and serological study after triple therapy of Helicobacter pylori-associated duodenal ulcer. AJG 89(10):1793-1796. 48. Lin SR,Lee YJ,Tsai JH,1994,Mutations of p53 gene in human functional adrenal neoplasms.J Clin Endocrinol Metab 78(2):483-491(correspond). 49. Chang CY,Chang LL,Lin SR,Chang SF,1993,Charaterization of R plasmid from Shigella sonnei in Taiwan.Kaohsiung J Med Sci 9:616-624. 50. Lee YJ,Lin SR,Tsai JH,1993,Increased adrenal medullary atrial natriuretic polypeptide synthesis in patients with primary aldosteronism.J Clin Endocrinol Metab 76(5):1357-1362. 51. Juan KH,Lin SR,etc,1992,Bacterial flora of the external ear canal-2.effect of irradiation.Kaohsiung J Med Sci 8(1):21-23. 52. Lin SR,etc,1992,Drug resistance and plasmid profile in Taiwan.J Epidemiol Infect 108:87-97(correspond). 53. Juan KH,Lin SR,etc,1990,Bacterial flora of the external ear canal-1.camcer patients. Kaohsiung J Med Sci 6(8),418-421. 54. Wang WM,Lin SR,etc,1990, Evaluation of urease test, gram stain, culture and histology in the detection.of Campylobacter pylori.J Formosa Med Assoc 89(8):683-686. 55. Yang SA,Lin SR,etc,1990,Pemphigus foliaceus with cryptococcemia caused by Cryptococcus albidus.Derm Sinica 8:31-35. 56. Lin SR,etc,1989,Isolation of Crytococcus albidus var.albidus in patient with pemphigus foliaceus.Kaohsiung J Med Sci.5(2):126-128. 57. Lin SR,etc,1988,In vitro antibacterial activity of cefixime.Kaohsiung J Med Sci 4(10):586-591 58. Lin SR,etc,1988,Molecular characterization of virulence plasmid in Shigella species.Kaohsiung J Med Sci 4(11):633-642 59. Peng CF,Lin SR,1987,Serotypes and antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa.Kaohsiung J Med Sci 3(5) :309-317.