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					CIRM Shared Research Laboratory Information Form – Part Two
Section A. Project Information
Project Title Shared facilities for the preclinical testing of embryonic stem cell therapies.
Limited to 300 Characters

Project Start Date Aug 1, 2007

Construction Start Date Nov 1, 2007

Occupancy Date

Jan 15, 2008

Total Part Two Funds Requested for Shared Laboratory Space Total Part Two Funds Requested for Stem Cell Techniques Course Total Capital Funds Requested

$ 295,888

$ 98,088

Note: All green fields are calculated values. Do not enter a value in the field.

Please indicate whether you propose to apply for funding of a Stem Cell Techniques Course along with the Shared Laboratory Space, or just the Shared Laboratory Space. Shared Research Laboratory only Shared Research Laboratory and Stem Cell Techniques Course

NOTE: Please be aware that any information you provide in this form will be made publically available.

Section A. 1. Program Director
Name Dr.
Prefix

Marcus
First

Oliver
Middle

Muench
Last Suffix

Email (office)

mmuench@bloodsystems.org CL1-00510-1

Application Number

This email address identifies you to CIRM. Please use this email address for all correspondence with CIRM. This field should fill automatically, based on the email address. If not, enter the number you received via email from CIRM, in the form “XX9-99999-9”, where “X” is a letter, and “9” is a digit.

Section A. 2. Facilities Contact
Name Professor
Prefix

Michael
First

P.
Middle

Busch
Last Suffix

Institution

Other
If your institution is not listed, please identify the name of the institution here.

Other Institution Blood Systems Research Institute Position Title Department Address 270 Masonic Ave. Director, Blood Systems Research Institute

City Phone Number Email (office)

San Francisco (415) 749-6615 mbusch@bloodsystems.org Ext Fax Number

CA

Zip Code 94118

(415) 567-5899

This email address identifies you to CIRM. Please use this email address for all correspondence with CIRM.

Shared Lab Grant Information Form Page 1

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CIRM Shared Research Laboratory Information Form – Part Two

Section A. 3. Public Abstract
See Appendix A.

Section A. 4. Statement of Benefit to California
See Appendix A.

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CIRM Shared Research Laboratory Information Form – Part Two
Section B. Laboratory Renovation Plan
Project Manager Title Brian Sellner Director of Facilities Construction Supervisor Brian Sellner Title Company/Institution Director of Facilities Blood Centers of the Pacific

Company/Institution Blood Centers of the Pacific

Describe plans for development/renovation of the shared laboratory space including fixed equipment costs. Include a description of the current space and how it will be renovated and reconfigured to form the laboratory. Include as attachments one 11x17 page of the current floor plan space and one 11x17 page of proposed floor plan of the renovated space. Describe all renovations that will be done. Describe how the project will be managed and tracked, as well as how change orders will be handled. For laboratories that are proposed to be located in leased space, provide information regarding the institution's longterm access to the leased space. Describe plans and schedule for all phases of development including design, construction, and installation of equipment leading to a functional laboratory. Give a proposed contingency plan in case of cost overruns. Any additional costs due to budget overruns will be the responsibility of the grant recipient. (narrative limited to 3 pages) The shared stem cell facilities at Blood Systems Research Institute (BSRI) will encompass four rooms: the main Stem Cell Laboratory, a secondary Tissue Culture room, a Cell Analysis Laboratory and the Mouse Vivarium. These four rooms are in existence, but require renovation to meet the proposed uses (see attached floor plan drawings). Until Fall 2006, these rooms were used as laboratory space for work done under contract for the National Marrow Donor Program (NMDP). Details of the renovations and equipment installations are described separately for each room below. Management Plan: A renovation committee will develop the initial project plan that will then be presented to all principle investigators and the Director of research at BSRI, Dr. Michael Busch. The renovation committee will consist of the program director, Dr. Marcus Muench, and key personnel responsible for running the shared facility, Dr. EJ Read, Dr. Alicia Bárcena and Mr. Dale Hirschkorn. Additionally, Dr. Leslie Tobler and Mr. Brian Sellner, who will serve as both the construction supervisor and the project manager, will also be included on the renovation committee. Together the renovation committee represents the scientific staff that will use and manage the shared facilities and surrounding laboratories as well as the Director of Facilities for Blood Centers of the Pacific, which contains BSRI, and the Director of Administration for BSRI. The renovation committee will consider and incorporate any reasonable changes to the renovation plan requested by the other BSRI scientific staff. Mr. Sellner will be responsible for procuring the preliminary plans. Hugh Cotter, an architect with Oculus Inc. in San Francisco, has been used on past laboratory construction projects at BSRI and is the lead candidate to prepare drawings for this proposal. The renovation committee will be responsible for approval of preliminary plans and working drawings. The project manager will be responsible for soliciting bids from contractors for the renovations. Mr. Sellner will work with the rest of the renovation committee to schedule construction activities and oversee the renovation process. The entire renovation committee will review any change orders that arise. The renovation committee will give approval for the changes if costs fall within the project budget. If the change order results in additional costs, then the committee will confer with Dr. Busch to approve the additional costs to be charged to BSRI. Stem Cell Laboratory: This laboratory was previously designed as a tissue culture laboratory, so minimal renovations are needed to convert this space for service as the main tissue culture laboratory for maintaining and expanding hESC lines and for testing clinical-grade culture technologies. One large (2-person) biosafety cabinet and two CO2 tissue culture incubators are currently present in the laboratory. A Miltenyi Biotec Clinimax cell separation device and a Coulter Z1 cell counter are also assigned to this laboratory. Additionally, extensive -80°C freezer and liquid nitrogen storage capacity, that was used for the NMDP tissue bank, is located adjacent to the Stem Cell Laboratory that will provide ample cell and tissue storage capacity for the shared facility. Renovations to be performed: Gas lines for nitrogen and carbon dioxide to supply tissue culture incubators will be installed, as well as a secure wall rack to hold the compressed gas tanks. A shelving unit will be moved to allow placement of two new biosafety cabinets. A wall-mounted work-bench will be installed. To maximize cleanliness and containment, modifications to the laboratory door will be made to allow self-closing. Walls will be patched and painted and emergency lighting installed. A wireless base station for computer networking will be installed. Fixed Equipment Purchases: Two single-person biosafety cabinets are being requested. These safety cabinets ($11,500 each) will be used for work related to culturing and banking hESC. We are also requesting a BioSpherix incubator ($8,000) capable of controlling both oxygen and carbon dioxide levels. Additionally we are requesting $18,500 for a Wave Bioreactor for large scale Shared Lab Grant Information Form Page 3 (Revised 03/07/2007)

CIRM Shared Research Laboratory Information Form – Part Two
Section B -- 1. Laboratory Renovation Plan (continued)
clinical expansion and culture of cells. A refrigerated table-top centrifuge ($14,000) will also be purchased. Tissue Culture Laboratory: This laboratory currently serves as a functional tissue culture room and requires only minor modifications and outfitting. The room contains two biosafety cabinets and two standard CO2 incubators. A desk is available for research staff. Cabinetry and a small storage room exist to stock supplies. Renovations to be performed: Shelving on a center island will be removed to increase the amount of level bench space for equipment and research activities. Electrical outlets will be dropped from the ceiling to the center island to provide electricity for equipment placed on the island. A wireless base station for computer networking will be installed. Walls will be patched and painted and emergency lighting installed. Fixed Equipment Purchases: A refrigerated table-top centrifuge ($14,000) will be purchased for tissue culture work. Cell Analysis Laboratory: Currently the laboratory that will serve as the Cell Analysis Laboratory has installed abundant bench space and cabinetry that prevents the installation of large equipment items. A small portion of the cabinetry with a sink installation will be preserved; the rest will be removed to provide room for large equipment items. BSRI has within the last year purchased the most costly pieces of laboratory equipment needed for the planned facility including a FACSAria cell sorter, a Leica MZ16F fluorescent stereomicroscope, a Leica DMI 6000B fluorescent-inverted DigitalMicroscope and a LABScan 100 Luminex analyzer. Additionally, a Cell-Dyn Cell analyzer and an LSR II flow cytometer will be relocated to this room. Renovations to be performed: To make space for the flow cytometry equipment, cabinetry and benches will be removed as well as a center island. Floor tiling will be repaired. Two doors will be installed to close off the room from an adjacent corridor and the Tissue Culture Laboratory. Alterations will be made to HVAC in the room to allow for negative pressure operation to prevent any airborne pathogens from contaminating the adjacent tissue culture and stem cell laboratory as well as improve air conditioning capacity to counteract the heat generated from the flow cytometers and other large equipment items. Additional electrical circuits will be added to support the large equipment. In one corner of the room, moveable black curtains will be hung from the ceiling to the floor to create a darkened enclosure for the fluorescent microscopes. A table will be installed to support the microscopes and computer equipment, including an isolation table to hold the inverted microscope. An overhead light in this area will need to be relocated. A wireless base station for computer networking will be installed. Walls will be patched and painted and emergency light installed. Fixed Equipment Purchases: We are requesting funds for a cryostat ($37,000) to be used for histological analysis of stem-cell derived tissues. Mouse Vivarium: Currently this laboratory contains ample work-benches and two small biosafety cabinets, one of which will be kept for sterile animal dissections. The room was originally constructed to be a vivarium, but was then converted to office space followed by conversion to laboratory space used for immunology research. Hence, the room is structurally designed to house animals. A floor drain is installed to simplify cleaning. The room has a hard-lid ceiling rather than the ceiling tiles in the adjacent rooms. A sink is installed in the laboratory and a small refrigerator with a freezer is in place to be used for reagent storage and temporary carcass disposal. An antechamber, complete with another hand-washing station, separates the vivarium from the hallway and provides a place to gown before entering the vivarium. Renovations to be performed: Cabinetry and benches will be removed from one side of the room as well as a center island. One of the biosafety cabinets will be removed (to be replace by a model suited for animal work). The old flooring will be replaced with vinyl flooring. Exhaust ducts for the cage racks and a moveable localized exhaust drop (to vent workspaces of anesthesia, carbon dioxide and animal odors) will be installed. To counteract the negative pressure due to active venting, a booster fan with a HEPA filter will be installed in the air supply duct and pressure monitor will be installed in the room. Automatic lighting and an override timer will be installed to provide diurnal lighting. Room temperature and humidity monitors will also be installed. Wall mounts to secure gas cylinders will be installed. A second door, used only for moving large equipment in and out of the room, will be sealed shut (but not removed) after the new equipment has been installed. A security card reader will be placed on the outside Shared Lab Grant Information Form Page 4 (Revised 03/07/2007)

CIRM Shared Research Laboratory Information Form – Part Two
Section B -- 1. Laboratory Renovation Plan (continued)
door of the antechamber to limit access to the room to authorized users. A wireless base station for computer networking will be installed. Walls will be patched and painted and an emergency light installed. Fixed Equipment Purchases: Three HEPA-filtered cage-racking systems capable of holding 504 cages are requested for a total of $37,000. A specialized biosafety cabinet ($14,000) is requested as a workspace for cage changes and experimentation. We also request a stereomicroscope mounted on an articulated arm ($25,000) used for mouse surgeries and dissection and an anesthesia work-station ($7,300). Contingency plan for cost overruns: BSRI general institutional funds will bear additional costs due to budget overruns. Timeline: The process of renovation can begin as soon as funding has been awarded. It is anticipated that the facility will be fully functional 6 months after the start of renovation, although the tissue culture rooms will likely be functional 1 to 2 months before this time as the renovations required for these rooms are minimal. Two months have been allotted for the process of developing and approving working drawings. During this period, accounting mechanisms will also be developed to assure that funding associated with the shared stem facility is not intermingled with any federal funding. Another month has been designated to advertise and award the construction contracts. Two and a half months have been allotted for construction activities with completion foreseen by mid January 2008. Equipment purchases will be made during the first four months of the award. Room for temporary storage of larger items is available adjacent to the shared facility until renovation has been completed. Notice of completion is expected 6 months after the notice of award.

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CIRM Shared Research Laboratory Information Form – Part Two

Section B. 1. Schedule/Timeline and Drawdown of Funds Table
Provide a realistic schedule and drawdown of funds for completing each activity/milestone, as indicated below.

# Activity/Milestone
1 Grant Award (estimate) Request for Planning Funds (10% of 2 Construction Costs) 3 Prepare Preliminary Plans 4 Approval of PPs 5 Prepare Working Drawings 6 Approval of WDs Request Construction Contract funds (80% 7 of Construction Costs) 8 Advertise for Construction Contract 9 Award Construction Contract 10 Construction Activities 11 Completion of Equipment Purchases 12 Request Equipment Purchase funds 13 Beneficial Occupancy 14 Notice of Completion Request Construction Completion Amount 15 (10% of Construction Funding)

Start Date

Completion or Milestone Date
Aug 1, 2007 Aug 6, 2007

Amount of CIRM funds to be drawn

$ 9,809

Aug 10, 2007

Aug 24, 2006 Sep 7, 2007

Sep 10, 2007

Sep 17, 2007 Oct 1, 2007 Oct 5, 2007 $ 78,470

Oct 1, 2007

Oct 22, 2007 Oct 26, 2007

Nov 1, 2007

Jan 15, 2008 Dec 3, 2007 Dec 17, 2007 Jan 15, 2008 Feb 1, 2008 Mar 15, 2008 $ 9,809 $197,800

“Preliminary Plans” (PPs) represent approximately 35 percent of the design effort, or may be considered the product of completing the "Design Development" (DDs) phase of architectural work. “Working Drawings” (WDs) represent drawings and specifications from which a contractor may determine the full extent of work contemplated in the project for purposes of submitting a bid; may be referred to as completion of “Construction Documents” (CDs) phase of architectural work.

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CIRM Shared Research Laboratory Information Form – Part Two

Section B. 2. Budget
Provide a complete budget for the renovation that includes construction costs, design fees, administration of the project, other costs (i.e. installation of equipment) and a construction contingency (limited to 7-10% of the construction budget). Identify the amount of CIRM funds requested and the matching funds (construction requires 20% matching funds). Provide a complete budget for movable equipment (equipment requires 20% matching funds). (narrative limited to 3 pages)
(Note: An Excel spreadsheet can be attached as long as the total submission for this Section is limited to 3 pages)

Overview: The total cost for the proposed renovations and equipment purchases is $295,888 (see attached spreadsheet). The cost of renovations is $86,335 and a 10% contingency fee has been added to the total. Design fees of $3,120 have been added based on an estimated 24 hours work at $130/hour. No administrative costs to supervise the project have been budgeted as the proposed activities fall under the standard budgeted duties assigned the Director of Facilities at our institution. Renovation Budget Justification: Unless otherwise noted, budget estimates were generated by Brian Sellner, Director of Facilities at our institute, who has extensive experience in performing and supervising renovations at our facility. Otherwise, contractors used for past projects were asked for estimates as indicated. It is anticipated that contractors will be hired to perform the renovations under supervision of Mr. Sellner and the renovation committee. Each of the rooms will have emergency battery lighting installed ($500/room) and the wall patched and painted for $500 to 1,500/room. Wireless networking will also be installed in each individual room as walls in the facility prevent good signal strength outside of each room based on preliminary testing. Our institute’s IT department offered an estimate of $500/room to run Ethernet cable to fixed computer systems and to set up wireless access points. Another $500 has been budgeted per room for moving costs. This cost covers expenses for labor to move large equipment in and out of the laboratories required to perform the renovations and painting. The other cost estimates are at or below $3000 for removal of cabinetry and minor renovations were all provided by Mr. Sellner. An electrical contractor was contacted and offered an estimate of $10,000 to bring four additional dedicated electrical circuits into the Cell Analysis Laboratory. All HVAC modifications throughout the facility, including installing the localized exhaust drop and environmental monitoring systems in the Mouse Vivarium, where provided by LINC Service (Airco Commercial Services), after touring the facility and discussions of our planned needs. Floortrends Inc. provided the estimate to furnish and install the vinyl flooring in the Mouse Vivarium. The cost of the isolation table for the inverted microscope was obtained after discussion with a sales representative. Equipment Budget Justification: All listed prices in the attached document (grey-shaded areas) were obtained by researching the products and obtaining a price quote. An additional 15% has been added to each item to account for taxes, shipping and any modest price increases that may occur prior to purchase. BSRI Matching Funds: BSRI has recently (Fall 2006) invested significant funds in equipment to expand its stem cell and cell therapy research programs. The following items were purchases in 2006 and will be located in the shared stem cell facility for common use: a FACSAria cell sorter ($384,893), a Leica MZ16F fluorescent stereomicroscope ($22,172), a Leica DMI 6000B fluorescent-inverted DigitalMicroscope ($82,281) and a LABScan 100 Luminex analyzer ($41,000). Total cost of these items is $530,346, which provides much greater than 20% matching funds for the proposed renovations and equipment purchases. All items were purchased with institutional funds and not with any extramural funding. Documentation to support these acquisitions is available. Drawdown Schedule: Requests for funding will occur at the indicated times as detailed on page 4 (section B.1).

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CIRM Shared Research Laboratory Information Form – Part Two
Section B. 3. Budget Summary Table
Complete the budget summary for the use of CIRM funds.
Note: All colored fields contain calculated data. Please do not enter anything in those fields.

Other Project Costs
Budget Category Construction Contract Costs Other Construction Costs (institutional) Subtotal Construction Design Fees Administrative Costs Construction Contingency Total Construction Movable Equipment Total Budget Total Budget $ 86,335 $ 000 $ 86,335 $ 3,120 $ 000 $ 8,633 $ 98,088 $ 197,800 $ 295,888 CIRM Grant Funds Institutional Match $ 86,335 $ 000 $ 86,335 $ 3,120 $ 000 $ 8,633 $ 98,088 $ 197,800 $ 295,888 $ 000 $ 000 $ 000 $ 000 $ 000 $ 000 $ 000 $ 000 $ 000

Gross Square Feet Assignable Square Feet

1432 1346

$ 68.50 $ 72.87

$ 68.50 $ 72.87

Const Costs/GSF Const Costs/ASF

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CIRM Shared Research Laboratory Information Form – Part Two

Section B. 4. Institutional Commitment
Provide a detailed description of the amount and source of matching funding for each request that requires matching funds. The requirement of matching funds can be satisfied if the institution can document funds, excluding other grant funds, committed to similar projects (i.e., renovation of lab space and equipment purchase) after January 1, 2005. Detail the use of the space after the three year period is completed. (narrative limited to 2 pages) BSRI underwent a $4.7 million expansion of its laboratory research facilities with construction completed in May 2005. This construction project was funded through a secured $5 million bond by Blood Systems Foundation, the financial entity under Blood Systems that provides for the underlying support of research at BSRI. Although the construction costs don’t qualify for matching funds for this proposal, over $1 million of the bond money was spent on equipment for the institute. Some of this equipment will be installed in the shared stem cell facility and qualifies as matching funds. The items are: a FACSAria cell sorter ($384,893), a Leica MZ16F fluorescent stereomicroscope ($22,172), a Leica DMI 6000B fluorescent-inverted DigitalMicroscope ($82,281) and a LABScan 100 Luminex analyzer ($41,000). Total cost of these items is $530,346, which provides much greater than 20% matching funds for the proposed renovations and equipment purchases. All items were purchased with institutional funds and not with any extramural funding. Documentation to support these acquisitions is available. BSRI is a subsidiary of Blood Systems, a non-profit organization and the nation’s second largest provider of blood products. Providing cellular therapies based on stem cell technology is seen as natural extension of the mission of blood banks. BSRI is committed to the development of cellular therapies based on stem cell technologies. As evidence of this commitment, the program director, Dr. Muench, and Dr. EJ Read were both hired in 2006 to expand stem cell research at BSRI. It is fully anticipated that the shared stem cell facility will continue to be used to perform basic and preclinical research on stem cell therapies after the 3-year funding period. Funding of this proposal will further strengthen and expand the stem cell research program at BSRI both in the near-term and long-term.

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CIRM Shared Research Laboratory Information Form – Part Two

Section C. Stem Cell Techniques Course (if applicable)
Based on the information provided in Part One of the application describing the course, include a justification of the additional space required and additional equipment requested, if any. Include additional square footage and provide as an attachment one 11x17 page of the proposed floor plan of the renovated space. (narrative limited to 1 page) Not Applicable.

Limit narrative to visible field area. Shared Lab Grant Information Form Page 10 (Revised 03/07/2007)

CIRM Shared Research Laboratory Information Form – Part Two

Section C. 1. Schedule and Drawdown of Funds Table (if applicable)
Provide a realistic schedule and drawdown of funds for completing each activity/milestone, as indicated below.

# Activity/Milestone
1 Grant Award (estimate) Request for Planning Funds (10% of Construction 2 Costs) 3 Prepare Preliminary Plans 4 Approval of PPs 5 Prepare Working Drawings 6 Approval of WDs Request Construction Contract funds (80% of 7 Construction Costs) 8 Advertise for Construction Contract 9 Award Construction Contract 10 Construction Activities 11 Completion of Additional Equipment Purchases 12 Request Additional Equipment Purchase funds 13 Beneficial Occupancy 14 Notice of Completion Request Construction Completion Amount (10% of 15 Construction Funding)

Start Date

Completion or Amount of CIRM Milestone Date funds to be drawn

$ 000

$ 000

$ 000

“Preliminary Plans” (PPs) represent approximately 35 percent of the design effort, or may be considered the product of completing the "Design Development" (DDs) phase of architectural work. “Working Drawings” (WDs) represent drawings and specifications from whicha contractor may determine the full extent of work contemplated in the project for purposes of submitting a bid; may be referred to as completion of “Construction Documents” (CDs) phase of architectural work. “Additional Equipment” represents equipment to be used for the Stem Cell Techniques Course.

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CIRM Shared Research Laboratory Information Form – Part Two

Section C. 2. Budget (if applicable)
Provide a complete budget for the additional renovation that includes construction costs, design fees, administration of the project, other costs (i.e. installation of equipment) and a construction contingency (limited to 7-10% of the construction budget). Identify the amount of CIRM funds requested and the matching funds (construction requires 20% matching funds). Provide a complete budget for additional movable equipment (equipment requires 20% matching funds). (narrative limited to 3 pages)
(Note: An Excel spreadsheet can be attached as long as the total submission for this Section is limited to 3 pages)

Not Applicable.

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CIRM Shared Research Laboratory Information Form – Part Two
Section C. 3. Budget Summary Table (if applicable)
Complete the budget summary for the use of CIRM funds.
Note: All colored fields contain calculated data. Please do not enter anything in those fields.

Other Project Costs
Budget Category Construction Contract Costs Other Construction Costs (institutional) Subtotal Construction Design Fees Administrative Costs Construction Contingency Total Construction Additional Movable Equipment Total Budget Total Budget CIRM Grant Funds Institutional Match

Gross Square Feet Assignable Square Feet

$ 0.00 $ 0.00

$ 0.00 $ 0.00

Const Costs/GSF Const Costs/ASF

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CIRM Shared Research Laboratory Information Form – Part Two

Section D. Signature Page
Complete, save, and print Part Two of the Shared Research Laboratory Grant Information. Submit electronic application as an email attachment to laboratory@cirm.ca.gov no later than 5:00pm PST on March 16, 2007. Mail* the original executed Part Two application and five (5) copies to: Shared Research Laboratory Grant Application California Institute for Regenerative Medicine 210 King Street San Francisco, CA 94107 *Mailing must be postmarked no later than March 16, 2007. Applications will not be accepted after these deadlines.

Project Start Date Aug 1, 2007

Construction Start Date Nov 1, 2007

Occupancy Date

Jan 15, 2008

Total Part Two Funds Requested for Shared Laboratory Space

$ 295,888

Total Part Two Funds Requested for Stem Cell Techniques Course

Total Capital Funds Requested

$ 98,088

Facilities Contact Professor Michael P. Busch Director, Blood Systems Research Institute Blood Systems Research Institute 270 Masonic Ave. San Francisco , CA 94118 (415) 749-6615 mbusch@bloodsystems.org

Authorized Organizational Official

Date

Print Name

Title

Program Director

Date

Print Name

Title Shared Lab Grant Information Form Page 14 (Revised 03/07/2007)

Program Director: MUENCH, Marcus

BSRI Renovation and Equipment Budget

Application #CL1-00510-1

Stem Cell Laboratory Renovations: Moving costs for renovation Door modifications Install gas lines & tank holders Install emergency lighting Install work bench Patch & Paint Walls Install computer wireless network Equipment: Sorvall Refrigerated Table-top centrifuge BioSpherix low oxygen tension incubator Wave Bioreactor 2 NuAire Biosafety cabinets Tissue Culture Laboratory Renovations: Moving costs for renovation Modify center island counter and install electrical outlets Install emergency lighting Patch & Paint Walls Install computer wireless network Equipment: Sorvall Refrigerated Table-top centrifuge Cell Analysis Laboratory Renovations: Moving costs for renovation Remove cabinetry and center island Repair Flooring Install dedicated electrical circuits for flow cytometers Install doors to isolate room Upgrade HVAC and provide negative air pressure Install dark room curtain track and curtain Relocate overhead light from above darkroom Table for steromicroscope and computer monitor VayTek Microscope vibration isolation table Install emergency lighting Patch & Paint Walls Install computer wireless network Equipment: Jencons Scientific Cryostat Mouse Vivarium Renovations: Moving costs for renovation Remove cabinetry and center island Install Armstong Medentech sheet vinyl flooring Seal second door CO2 Tank wall mount Upgrade HVAC with HEPA filtration and add animal rack venting Install locallized exhaust drop Install automated light system, humidity and temperature gauges Install emergency lighting Patch & Paint Walls Install security card reader on outer antechamber door Install computer wireless network Equipment: 3 Innovive cage racks with air vent adaptors NuAire Animal Transfer Station Anesthesia work station & vaporizer Leica Stereomicroscope and articulated arm stand

Itemized Costs $500.00 $200.00 $1,000.00 $500.00 $2,000.00 $1,000.00 $500.00 $14,000.00 $8,000.00 $18,500.00 $23,000.00

Renovation Subtotal

Equipment Subtotal

$5,700.00

$63,500.00

$500.00 $2,500.00 $500.00 $500.00 $500.00 $14,000.00

$4,500.00 $14,000.00

$500.00 $3,000.00 $500.00 $10,000.00 $6,000.00 $20,000.00 $1,000.00 $1,000.00 $1,500.00 $2,300.00 $500.00 $1,500.00 $500.00 $37,000.00

$48,300.00 $37,000.00

$500.00 $3,000.00 $5,135.00 $500.00 $500.00 $10,000.00 $1,200.00 $2,000.00 $500.00 $1,500.00 $2,500.00 $500.00 $37,000.00 $14,000.00 $7,300.00 $25,000.00

$27,835.00

$83,300.00 $197,800.00 $86,335.00 $8,633.00 $3,120.00 $295,888.00

Equipment Budget Renovation Budget Constuction Contigency Design Fees Total Institutional Matching Funds (Purchased Fall 2006) FACSAria cell sorter Leica MZ16F fluorescent stereomicroscope Leica DMI 6000B fluorescent-inverted DigitalMicroscope LABScan 100 Luminex analyzer Total

$384,893.00 $22,172.00 $82,281.00 $41,000.00 $530,346.00

CIRM Shared Research Laboratory Information Form – Part Two Supplement
Project Information
Application Number CL1-00510-1 Program Director Name:

Historical Performance
Provide information on past performance for 3 projects.

Project 1

Project 2

Project 3

BSRI Laboratories Buildout BCP Research Renovations

Brief Project Title Original Budget (Total project cost) Final project cost Scheduled Completion Date Actual Notice of Completion Date Gross Square Feet involved Assignable Square Feet involved Approximate number of change orders Value of all change orders & claims Type of construction management Design-Bid-Build $3,102,836 $3,335,394 Apr 1, 2005 May 1, 2005 11,499 8,233 40 $ 232,558 In-house Forces $ 150,000 $ 150,000 Dec 31, 2004 Dec 31, 2004 3,479 3,010 0 $ 000

Laboratory Alteration Projects
Please enter the number of laboratory alteration projects completed by the applicant in the past 2 years (in the range of $1-5 million in project cost), and the approximate total dollar value that these projects represent. Total Laboratory Alteration Projects 1 Approximate Total Value $3,335,394

Limit Budget Justification to visible field area. Shared Research Laboratory Information Form Part One Supplement Page 1

Blood Systems Research Institute Program Director: Marcus Muench Application # CL1-00510-1 CURRENT FLOORPLAN

Blood Systems Research Institute Program Director: Marcus Muench Application # CL1-00510-1 PROPOSED FLOORPLAN

Appendix A
Application: CL1-00510-1 Title: Shared facilities for the preclinical testing of embryonic stem cell therapies. Public Abstract:
Blood Systems Research Institute (BSRI), located in San Francisco, has a 45-year history of research in transfusion medicine and related fields. BSRI has undergone a recent expansion in investigators, laboratory space and equipment to address the growing number of opportunities that exist in the broad field of cellular therapeutics, particularly in the area of human embryonic stem cell (hESC) technology. The notable strength of BSRI lies in the expertise of its investigators in clinical and translational research owing to its long history with cellular therapy in the form of blood cell transfusion. Laboratory space at BSRI will be remodeled to support our program in hESC research. We request funds to improve two tissue culture rooms to support basic research and pre-clinical studies on hESC lines. A 3rd laboratory will be converted into a cell isolation and analysis laboratory housing equipment used in the isolation and analysis of hESC-derived cells and tissues. A 4th room will be converted into a vivarium to study stem cell transplantation. The research facility will be used by scientists at BSRI and neighboring institutions in the San Francisco Bay Area to perform basic research and to develop methodologies appropriate for the clinical of application of hESC-based therapies. Therapies under investigations include developing blood and liver stem cells as a new source of tissues to treat birth defects and disease. A training program for research technicians is also included which will help train new workers in the stem cell field.

Statement of Benefit to California:
The shared stem cell facility will help develop a new cell therapies such as those based on blood and liver cells. Blood diseases such as sickle cell anemia and thalassemia as well as liver diseases caused by viral infection, drugs or inherited disease affect many thousands of Californians. Often, transplanting healthy cells offers treatment or a cure for many of these diseases, but a lack of available or suitable donor tissue prevents such therapy in many cases. Embryonic stem cells offer the hope of generating a sufficient supply of tissues for cellular therapy. The successful outcome of this work will offer new hope to many Californians suffering from blood or liver diseases. This will improve lives and save money on long-term health care costs associated with these diseases. Additionally, a training program has been included to help train research technicians for work in the stem cell field, therby helping to creat high paying jobs and provide the workforce needed for California to be the leader in stem cell research.

RFA 07-01 CIRM Shared Research Laboratories