Making A Karyotype Materials Needed Scissors Tape or Glue Stick The metaphase plate of Geimsa-stained human chromosomes has been photographed and reproduced on the last page of this handout. A karyotype of human G-banded chromosomes and a diagrammatic representation of the homologous chromosomes are below. (Only one of each of the homologues is shown in the diagrammatic representation.) You will note that each of the human chromosome pairs is numbered and similarly shaped chromosome pairs are clustered in the karyotype. This convention is used to help identify specific human genetic disorders. Down Syndrome, for example, is a trisomy 21, having three copies of the number 21 chromosome. Cut out each of the chromosomes from the metaphase plate illustration and make your own karyotype on the page provided at the end of this exercise. You will first match the homologous pairs, and then align them according to their chromosome number. Glue or tape your chromosomes on the page. Turn in your completed karyotype to your instructor.
Diagram of G-banded Chromosomes Making a Human Karyotype