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TNS RECOVERY COMPLEX® COMPETITIVE ANALYSIS Enzyme Linked ImmunoSorbent Assays (ELISA1) were used to quantify the growth factors Growth Factor/Cytokine Tested TNS Reluma BRC and cytokines and a colorimetric assay was used for antioxidant status for the following three bFGF (FGF-2) * * products: KGF (FGF-7) * * HGF * * • TNS Recovery Complex with NouriCel-MD® PDGF AA * * • Reluma (Invitrx) IGF1 * * • Bio-restorative Cream with PSP® (Neocutis) TGFß1 * TGFß2 * * Results TGFß3 * VEGF * • TNS Recovery Complex contained a larger number of stable growth factors than Reluma. IL6 * * • Bio-restorative Cream with PSP (BRC) contained no stable cytokines or growth factors that IL7 * * can be detected using a standard extraction procedure. IL8 * * • Antioxidants were observed in all three formulations at similar concentrations. GM-CSF * * Growth Factors/Cytokines detected by ELISA Analyses were only for specific growth factors/cytokines intended to cover the range of com- * Levels were below the detection limits of ELISA ponents claimed by competitors and performed independently by Synthasome Inc.2 In a separate study performed by BioSource Cytokines and Signaling, a division of Invitrogen Corporation3, concentrations of VEGF, HGF and TGFß were determined for the following four products: 10000 Neocutis Cream • TNS Recovery Complex with NouriCel-MD TNS Recovery Complex • CRS with Growth Factor (Topix) 8000 Topix CRS • Bio-restorative Cream with PSP (Neocutis) Neocutis Biogel C oncentration (pg/mL) • Bio-gel with PSP (Neocutis) 6000 Sample Preparation • 15 different extraction protocols were evaluated for extraction of proteins from the sam- ples. The protocol giving the best recovery across all products was selected for extraction 4000 of samples. • All the contract laboratory personnel were blinded to the identity of the four products be- ing tested. 2000 Results • TNS Recovery Complex contains a much higher concentration of all the measured pro- 0 teins than any of the other products. VEGF HGF TGFb • Only low levels of TGFß were found in the other three products. 1: ELISA is designed to detect and quantify substances such as peptides, proteins, antibodies and hormones. 2: Synthasome is a biotechnology company developing products for regenerative medicine using tissue engineering technologies. 3: In-Vitrogen is a world leader in bio-molecular analysis techniques. SkinMedica Research Report Studies sponsored by SkinMedica and conducted at Synthasome Inc and Invitrogen Corporation.
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