TNS RECOVERY COMPLEX® COMPETITIVE ANALYSIS SkinMedica Research Report by happo5

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									TNS RECOVERY COMPLEX® COMPETITIVE ANALYSIS

Enzyme Linked ImmunoSorbent Assays (ELISA1) were used to quantify the growth factors                                                              Growth Factor/Cytokine Tested        TNS      Reluma        BRC
and cytokines and a colorimetric assay was used for antioxidant status for the following three                                                    bFGF (FGF-2)                                       *           *
products:                                                                                                                                         KGF (FGF-7)                                        *           *
                                                                                                                                                  HGF                                                *           *
• TNS Recovery Complex with NouriCel-MD®                                                                                                          PDGF AA                                            *           *
• Reluma (Invitrx)                                                                                                                                IGF1                                               *           *
• Bio-restorative Cream with PSP® (Neocutis)                                                                                                      TGFß1                                                         *
                                                                                                                                                  TGFß2                                              *           *

Results
                                                                                                                                                  TGFß3                                                         *
                                                                                                                                                  VEGF                                                          *
 • TNS Recovery Complex contained a larger number of stable growth factors than Reluma.                                                           IL6                                                *           *
 • Bio-restorative Cream with PSP (BRC) contained no stable cytokines or growth factors that                                                      IL7                                                *           *
   can be detected using a standard extraction procedure.                                                                                         IL8                                                *           *
 • Antioxidants were observed in all three formulations at similar concentrations.                                                                GM-CSF                                             *           *

                                                                                                                                                   Growth Factors/Cytokines detected by ELISA
Analyses were only for specific growth factors/cytokines intended to cover the range of com-                                                      * Levels were below the detection limits of ELISA
ponents claimed by competitors and performed independently by Synthasome Inc.2

In a separate study performed by BioSource Cytokines and Signaling, a division of Invitrogen
Corporation3, concentrations of VEGF, HGF and TGFß were determined for the following four
products:                                                                                                                           10000

                                                                                                                                                                                                      Neocutis Cream
• TNS Recovery Complex with NouriCel-MD
                                                                                                                                                                                                      TNS Recovery Complex
• CRS with Growth Factor (Topix)                                                                                                           8000
                                                                                                                                                                                                      Topix CRS
• Bio-restorative Cream with PSP (Neocutis)
                                                                                                                                                                                                      Neocutis Biogel




                                                                                                                  C oncentration (pg/mL)
• Bio-gel with PSP (Neocutis)
                                                                                                                                           6000
Sample Preparation
 • 15 different extraction protocols were evaluated for extraction of proteins from the sam-
   ples. The protocol giving the best recovery across all products was selected for extraction                                             4000
   of samples.
 • All the contract laboratory personnel were blinded to the identity of the four products be-
   ing tested.                                                                                                                             2000

Results
 • TNS Recovery Complex contains a much higher concentration of all the measured pro-
                                                                                                                                             0
   teins than any of the other products.                                                                                                                 VEGF                      HGF                        TGFb
 • Only low levels of TGFß were found in the other three products.
1: ELISA is designed to detect and quantify substances such as peptides, proteins, antibodies and hormones.
2: Synthasome is a biotechnology company developing products for regenerative medicine using tissue engineering technologies.
3: In-Vitrogen is a world leader in bio-molecular analysis techniques.



SkinMedica Research Report                                                                       Studies sponsored by SkinMedica and conducted at Synthasome Inc and Invitrogen Corporation.

								
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