Vigilância e controle de doenças infecciosas e parasitárias
VII SI I VIISI3
ESTUDO DA OCORRÊNCIA DE FILARIOSE BANCROFTIANA NO THE "PRESSURE PAN" EVOLUTION OF HUMAN ERYTHROVIRUS
MUNiCíPIO DE GUAJARÁ MIRIM, RONDÔNIA, BRASIL. BI9INTHEAMAZON,BRAZIL.
PEREIRA. C.G.'; KORTE, R.L.'; COSTA, K.O.3; SANTOS, R.V.'; SANTOS, B.C.'; CA- FREITAS, R.B.'; DURIGON, E.L.'; OUVEIRA, D.S.'; ROMANO. C.M.'; FREITAS,
MARGO,j.A.'; FONTES, G.'; CAMARGO, L.M.A.'. M.R.C.s; UNHARES,A.C.'; MELO, ~L.7; ZANOTTO, P.M.'.
I.Faculdade Sãa Lucas, Mante Negra, Ra, Brasil; 2.Fsllicb5usp, Parta Velha, Ra, Brasil; 3,5,6. t,J,S.lnstituto Evondro Chogos,Anonindeua, Po, Brasil; 2,4,6, 7,8.Universidode de São Paulo,
Fsl, Parta Velha, Ra, Brasi/; 4,7.UFAL, Maceió,A/, Brasil;8.lcb5usp, Mante Negra, Ra, Brasil. São Paulo, Sp, Brasil.
Introdução: Guajará-Mirim (RO), nunca constou como área de ocorrência de Introduction:The erythrovirus B 19, a human-associated member of the family Par-
transmissão ativa de filariose bancroftiana no Brasil, ao contrário de outras cidades vaviridae,is the only known human pathogenic erythrovirus and has been detected
amazônicas como Manaus e Belém que já foram focos de transmissão. O único estu- globally in human populations. This virus causes a wide spectrum of dinical condi-
do relatado sobre a prevalência de filariose bancroftiana em Rondônia na região de tions, mainly erythema infectious in children and arthropathy/arthralgia in adults.
Guajará-Mirim, data de 1953, por RACHOU, sendo que o único caso encontrado era Objective: To understand the evolutionary dynamics of human parvovirus B 19,
alóctone. Objetivos: a-) verificar a ocorrência de filariose bancroftiana em Guajará analyzingVPI and VP2 gene sequences of BI9 sampled from Belém (Amuon), the
Mirim, b-) determinar a infecção natural de mosquitos da espécie Culex quinque- city of São Paulo, Brazil and globally. Material and Methods:Viral DNA was ex-
fasciatus por W. bancrofti em Guajará Mirim-RO, utilizando a Reação em Cadeia da tracted from serum samples by the phenol-chloroform method. The PCR and semi-
Polimerase (PCR) Materiais e Métodos: a-) em humanos: pesquisa hemoscópica pelo nested PCR were performed and positive samples were sequenced by the dideoxy
método da gota espessa, na população de área de risco da cidade de Guajará Mirim, chain termination method. To compare B 19 from different regions we inferred maxi-
realizadas das 22 ás OI h. b-) em mosquitos: captura de mosquitos por xenomoni- mum likelihood phylogenetic trees using PAUR using the best-fit evolutionary model,
toramento nas áreas de risco das 07 ás 10 horas e determinação da infecção dos as determined by Modeltest 3.7.To determine the selective pressures acting on BI9
mosquitos por W bancrofti pela técnica da PCR. Resultados: até a presente data virus we performed the Tajima\'s D statistic and cates of nonsynonymous (dN) and
foram realizadas pesquisas hemoscópicas em 169 pacientes no Bairro Triângulo (de synonymous (dS) substitutions per site (ratio dN/dS) using the MEGA v3.1 package.
um total de 320 pessoas residentes), não sendo encontrado paciente portador de We also inferred site-specific dN and dS using both the CODEML and HyPhy pro-
filariose. Na pesquisa de vetores foram capturados e já processados pelo método da grams. To estimate cates of evolutionary change of B 19 in both Brazilian populations
PCR um total de 299 mosquitos fêmeas, sendo 281 Culex quinquefasciatus e todos we employed the Bayesian-Markov Chain-Monte Carlo (MCMC) method available
negativos. Conclusão: pelos dados apresentados, podemos afirmar, até o momento, in the BEAST v 1.3 package. Results: Our results support the hypothesis of multi-
que não há a ocorrência de filariose bancroftiana na cidade de Guajará Mirim. O es- pie and independent introductions of genotype I lineages into the Amazon region.
tudo deve-se ampliar para a abordagem de 1.000 habitantes, incluindo outros bairros The analysis revealed strikingly different patterns of evolutionary change for those
e a captura de 3.000 mosquitos. Apoio: FAPESP2007/00848-9 virallineages introduced in Belém, which exhibited a higher cate of nonsynonymous
substitutions compared to those viruses sampled from other locations. We propose
that such a difference is due to the high prevalence of B 19 in Belém (up to 85%)
compared to other locations (prevalences of approximately SO%),which imposes a
more intense selection pressure. Conclusion: Hence, those BI9 lineages introdu-
ASEPTIC MENINGITISASSOCIATEDWITH PARVOVIRUS BI9INFEC- ced in Belém experienced an elevated cate of amino acid change, driven by positive
TION IN PATIENTS FROM BELÉM, BRAZIL. selection, in arder to generate serial re-infections in a small web of transmission,
FREITAS, M.R.C.; FREITAS, R.B.; GOMES, M.L.C.; LlNHARES,AC which can be thought of as an evolutionary"pressure pan".
Instituto Evondro Chogos, Anonindeuo, Po, Brasil.
Introduction:The parvovirus BI9 was discovered in the England by Cossart et ai. VIISI4
(I 975). After its discovery, seroepidemiology studies were developed in temperate MOLECULAR CHARACTERIZATION OF HUMAN ERYTHROVIRUS
and tropical climate countries, showing its worldwide dispersion across age groups B 19 STRAINS OBTAINED FROM PATIENTS WITH SEVERAL CLINI-
and in both sexes. Studies related to its pathogeny showed that the virus is as- CAL PRESENTATIONS INTHEAMAZON REGION OF BRAZIL.
sociated with erythema infectiosum, an exanthematous disease that affects mainly
FREITAS, R.B.'; MELO, F.L.'; OLIVEIRA, D.S.'; ROMANO, C.M.'; FREITAS, M.R.C.5;
children less than 10 years ord. In addition, this virus causes transient aplastic crisis,
LINHARES,A.C.'; ZANOTTO, ~M.7; DURIGON, E.L'.
particularly in patients suffering from hemolytic chronic anemia, arthropathy, hepati-
1,3,5.lnstituto Evandro Chagas,Ananindeua,Pa,Brasil; 2,4,6, 7,8.Universidade de São Paulo,
tis, meningitis, and meningoencephalitis in immunossupressed individuais and intrau-
terine infections leading to hydrops fetalis and even fetal death.ln this study, the main São Paulo,Sp,Brasil.
objective was to assess the possible association between parvovirus B 19 infections
and aseptic meningitis. Material and methods:The clinical samples (LCR) were col- Introdution: Human erythrovirus Bl9,endemic in theAmazon region since 1990, is
lected in the Health Basic Unit of Pedreira district, 5ecretary of Public Health, Para associated with a wide spectrum of clinical presentations. Objectives:To assess the
5tate. The samples were examined by ELlSA to the capture of detect IgM and IgG prevalence of erythrovirus B 19 infection and the relative frequency of erythrovirus
antibodies. With regards to the molecular biology, was utilized the polymerase chain B 19 genotypes in patients in the Amazon region with various clinical presentations.
reaction (PCR)/nested PCR to detect parvovirus BI9 DNA. Results:A total of 144 Material and Methods: A total of 487 clinical samples obtained from patients
clinical samples were examined with 49.3% (71/144) of samples belonging to patients with symptoms suggestive of erythrovirus infection were tested using specific IgM
with symptoms suggestive of aseptic meningitis, including fever, headache, vomits, and IgG antibody assays (ELlSA) and PCR for viral DNA detection. Partial VP 1 and
neck stiffness. There were 73 (50.7%) out of 144 samples which belonged to the VP2 regions were sequenced and genotyped by phylogenetic reconstruction. Re-
contrai group, including patients with symptoms other than those typical of aseptic sults: BI9 DNA was detected in 117 (24%) of 487 samples. Df these, 106 (91%)
meningitis. The cate of parvovirus BI9 recent infection (antibody IgM positive andl isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found.
or DNA detection) was of 11.3% (8/71) among patients with symptoms and signs Genotype 1 had three clusters (AI,A2 and B) and ali genotype 3 sequences were
suggestive of aseptic meningitis and of 1.4% (1/73) those patients assigned to the subtype 3b.AII patients with hematological disorders within cluster B of genotype I
control-group. There was a significant difference between these two groups: 11.3% were infected by the same B 19 lineage, suggesting that this lineage of B 19 mar have
(8/71) and 1.4% (1/73) [P=O.OI] for patients with aseptic meningitis and contrais, been transmitted via transfusion of blood products. Conclusion:We reported two
respectively. Other viruses, bacteria, parasite and fungi were ruled out as potential genotypes. 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon
etiological agents. Conclusion: Although preliminary, our data strongly suggest that region during the past 10 years.
parvovirus B 19 mar piar a role as a cause of aseptic meningitis in the Amazon region
and serve as an alert for health professionals.
462 4S0 Congresso da Sociedade Brasileira de Medicina Tropical