Vigilância e controle de doenças infecciosas e parasitárias

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					Vigilância e controle de doenças infecciosas e parasitárias




VII SI I                                                                                                      VIISI3

ESTUDO           DA      OCORRÊNCIA            DE    FILARIOSE             BANCROFTIANA                 NO    THE      "PRESSURE                   PAN"          EVOLUTION                 OF        HUMAN              ERYTHROVIRUS
MUNiCíPIO              DE   GUAJARÁ       MIRIM,     RONDÔNIA,             BRASIL.                            BI9INTHEAMAZON,BRAZIL.
PEREIRA.      C.G.';    KORTE,   R.L.';   COSTA,    K.O.3; SANTOS,         R.V.'; SANTOS,      B.C.';   CA-   FREITAS,         R.B.';     DURIGON,             E.L.';     OUVEIRA,            D.S.';       ROMANO.              C.M.';       FREITAS,
MARGO,j.A.';           FONTES,   G.'; CAMARGO,        L.M.A.'.                                                M.R.C.s; UNHARES,A.C.';                     MELO,         ~L.7; ZANOTTO,              P.M.'.

I.Faculdade     Sãa Lucas, Mante      Negra, Ra, Brasil; 2.Fsllicb5usp,     Parta Velha, Ra, Brasil; 3,5,6.   t,J,S.lnstituto      Evondro Chogos,Anonindeua,                   Po, Brasil; 2,4,6, 7,8.Universidode                     de São Paulo,
Fsl, Parta Velha, Ra, Brasi/; 4,7.UFAL, Maceió,A/,     Brasil;8.lcb5usp,     Mante    Negra, Ra, Brasil.      São Paulo, Sp, Brasil.



Introdução:    Guajará-Mirim (RO), nunca constou como área de ocorrência de                                   Introduction:The               erythrovirus            B 19, a human-associated member of the family Par-
transmissão ativa de filariose bancroftiana no Brasil, ao contrário de outras cidades                          vaviridae,is the only known human pathogenic erythrovirus and has been detected
amazônicas como Manaus e Belém que já foram focos de transmissão. O único estu-                               globally in human populations. This virus causes a wide spectrum of dinical condi-
do relatado sobre a prevalência de filariose bancroftiana em Rondônia na região de                            tions, mainly erythema infectious in children and arthropathy/arthralgia in adults.
Guajará-Mirim, data de 1953, por RACHOU, sendo que o único caso encontrado era                                Objective: To understand the evolutionary dynamics of human parvovirus B 19,
alóctone. Objetivos: a-) verificar a ocorrência de filariose bancroftiana em Guajará                          analyzingVPI and VP2 gene sequences of BI9 sampled from Belém (Amuon), the
Mirim, b-) determinar a infecção natural de mosquitos da espécie Culex quinque-                               city of São Paulo, Brazil and globally. Material and Methods:Viral  DNA was ex-
fasciatus por W. bancrofti em Guajará Mirim-RO, utilizando a Reação em Cadeia da                              tracted from serum samples by the phenol-chloroform method. The PCR and semi-
Polimerase (PCR) Materiais e Métodos: a-) em humanos: pesquisa hemoscópica pelo                               nested PCR were performed and positive samples were sequenced by the dideoxy
método da gota espessa, na população de área de risco da cidade de Guajará Mirim,                             chain termination method. To compare B 19 from different regions we inferred maxi-
realizadas das 22 ás OI h. b-) em mosquitos: captura de mosquitos por xenomoni-                               mum likelihood phylogenetic trees using PAUR using the best-fit evolutionary model,
toramento nas áreas de risco das 07 ás 10 horas e determinação da infecção dos                                as determined by Modeltest 3.7.To determine the selective pressures acting on BI9
mosquitos por W bancrofti pela técnica da PCR. Resultados: até a presente data                                virus we performed the Tajima\'s D statistic and cates of nonsynonymous (dN) and
foram realizadas pesquisas hemoscópicas em 169 pacientes no Bairro Triângulo (de                              synonymous (dS) substitutions per site (ratio dN/dS) using the MEGA v3.1 package.
um total de 320 pessoas residentes), não sendo encontrado paciente portador de                                We also inferred site-specific dN and dS using both the CODEML and HyPhy pro-
filariose. Na pesquisa de vetores foram capturados e já processados pelo método da                            grams. To estimate cates of evolutionary change of B 19 in both Brazilian populations
PCR um total de 299 mosquitos fêmeas, sendo 281 Culex quinquefasciatus e todos                                we employed the Bayesian-Markov Chain-Monte Carlo (MCMC) method available
negativos. Conclusão:  pelos dados apresentados, podemos afirmar, até o momento,                              in the BEAST v 1.3 package. Results: Our results support the hypothesis of multi-
que não há a ocorrência de filariose bancroftiana na cidade de Guajará Mirim. O es-                           pie and independent introductions of genotype I lineages into the Amazon region.
tudo deve-se ampliar para a abordagem de 1.000 habitantes, incluindo outros bairros                           The analysis revealed strikingly different patterns of evolutionary change for those
e a captura de 3.000 mosquitos. Apoio: FAPESP2007/00848-9                                                     virallineages introduced in Belém, which exhibited a higher cate of nonsynonymous
                                                                                                              substitutions compared to those viruses sampled from other locations. We propose
                                                                                                              that such a difference is due to the high prevalence of B 19 in Belém (up to 85%)
                                                                                                              compared to other locations (prevalences of approximately SO%),which imposes a
VI1512
                                                                                                              more intense selection pressure. Conclusion:                                    Hence, those BI9 lineages introdu-
ASEPTIC         MENINGITISASSOCIATEDWITH                            PARVOVIRUS             BI9INFEC-          ced in Belém experienced an elevated cate of amino acid change, driven by positive
TION        IN PATIENTS          FROM BELÉM,           BRAZIL.                                                selection, in arder to generate serial re-infections in a small web of transmission,
FREITAS, M.R.C.; FREITAS, R.B.; GOMES, M.L.C.; LlNHARES,AC                                                    which can be thought of as an evolutionary"pressure                                         pan".
Instituto   Evondro     Chogos, Anonindeuo,   Po, Brasil.



Introduction:The parvovirus BI9 was discovered in the England by Cossart et ai.                               VIISI4
(I 975). After its discovery, seroepidemiology studies were developed in temperate                            MOLECULAR                    CHARACTERIZATION     OF HUMAN                                                ERYTHROVIRUS
and tropical climate countries, showing its worldwide dispersion across age groups                            B 19 STRAINS                 OBTAINED   FROM PATIENTS WITH                                                SEVERAL  CLINI-
and in both sexes. Studies related to its pathogeny showed that the virus is as-                              CAL PRESENTATIONS                               INTHEAMAZON                          REGION             OF BRAZIL.
sociated with erythema infectiosum, an exanthematous disease that affects mainly
                                                                                                              FREITAS, R.B.'; MELO, F.L.'; OLIVEIRA, D.S.'; ROMANO, C.M.'; FREITAS, M.R.C.5;
 children less than 10 years ord. In addition, this virus causes transient aplastic crisis,
                                                                                                              LINHARES,A.C.'; ZANOTTO, ~M.7; DURIGON, E.L'.
particularly in patients suffering from hemolytic chronic anemia, arthropathy, hepati-
                                                                                                               1,3,5.lnstituto Evandro Chagas,Ananindeua,Pa,Brasil; 2,4,6, 7,8.Universidade de São Paulo,
tis, meningitis, and meningoencephalitis in immunossupressed individuais and intrau-
terine infections leading to hydrops fetalis and even fetal death.ln this study, the main                     São Paulo,Sp,Brasil.
objective was to assess the possible association between parvovirus B 19 infections
and aseptic meningitis. Material and methods:The clinical samples (LCR) were col-                             Introdution:              Human       erythrovirus         Bl9,endemic              in theAmazon               region    since      1990, is
lected in the Health Basic Unit of Pedreira district, 5ecretary of Public Health, Para                        associated        with      a wide     spectrum          of clinical    presentations.            Objectives:To                assess the
5tate. The samples were examined by ELlSA to the capture of detect IgM and IgG                                prevalence         of erythrovirus             B 19 infection          and the relative           frequency         of erythrovirus
antibodies. With regards to the molecular biology, was utilized the polymerase chain                          B 19 genotypes             in patients         in the Amazon           region       with     various     clinical       presentations.
reaction (PCR)/nested PCR to detect parvovirus BI9 DNA. Results:A total of 144                                Material          and       Methods:           A total      of 487       clinical     samples          obtained         from     patients
clinical samples were examined with 49.3% (71/144) of samples belonging to patients                           with     symptoms           suggestive         of erythrovirus           infection         were      tested      using specific         IgM
with symptoms suggestive of aseptic meningitis, including fever, headache, vomits,                            and IgG antibody              assays (ELlSA)            and PCR         for viral      DNA        detection.         Partial VP 1 and
neck stiffness. There were 73 (50.7%) out of 144 samples which belonged to the                                VP2 regions           were     sequenced            and genotyped             by phylogenetic              reconstruction.              Re-
contrai group, including patients with symptoms other than those typical of aseptic                           sults:     BI9     DNA        was detected             in 117 (24%)          of 487 samples.              Df      these,       106 (91%)
meningitis. The cate of parvovirus BI9 recent infection (antibody IgM positive andl                           isolates    were          genotype      1 and 11 (9%) were                genotype           3. No genotype               2 was found.
or DNA detection) was of 11.3% (8/71) among patients with symptoms and signs                                  Genotype           1 had three          clusters       (AI,A2      and B) and ali genotype                       3 sequences          were
suggestive of aseptic meningitis and of 1.4% (1/73) those patients assigned to the                            subtype     3b.AII         patients     with     hematological           disorders          within     cluster      B of genotype              I
control-group. There was a significant difference between these two groups: 11.3%                             were     infected         by the same B 19 lineage, suggesting                        that this lineage of B 19 mar have
(8/71) and 1.4% (1/73) [P=O.OI] for patients with aseptic meningitis and contrais,                            been transmitted              via transfusion           of blood       products.           Conclusion:We                 reported       two
respectively. Other viruses, bacteria, parasite and fungi were ruled out as potential                         genotypes.         1 and 3b, with              three      genotype       1 clusters          co-circulating             in the Amazon
etiological agents. Conclusion: Although preliminary, our data strongly suggest that                          region     during         the past 10 years.
 parvovirus B 19 mar piar a role as a cause of aseptic meningitis in the Amazon region
and serve as an alert for health professionals.




 462                                                                                                                                                                 4S0 Congresso         da Sociedade         Brasileira      de Medicina       Tropical