Sampling Instructions for EFB 493 1. Each group is assigned one main transect to sample – see Cara’s email if you forgot your group’s number. There is also a map online if you do not know the relative starting locations of transects. 2. From the start of your transect, measure/pace out 100 m at a heading of 270˚ (i.e. head west). 3. Once you have reached 100 m, construct 2 perpendicular “wing” transects (one north and one south), each 100 m in length. (1 data sheet per wing transect) 4. At 0 m, 50 m, and 100 m you will take tree measurements (NOTE: You will only do the sample plot for 0 m ONCE since this is where your two transects unite). If your sample plot is in the open, neglect these steps. These include: a. Point-centered-quarter i. Construct quartiles and measure 2 trees per quartile. Record: 1. Species 2. Circumference (cm) 3. Distance from center point (cm) ii. Also measure the height of the lowest branch of the two nearest conifers in each quartile and record the angle using the protractor. If no conifer is found within 25 m, make note that none could be found. b. Tally number of mature (overstory) aspen found in all 4 quartiles i. Try to obtain a twig (with buds) so that the sex of the tree can be determined. If this is impossible, then just make note. If a twig can be obtained, then label which transect and plot it came from. 5. Every 10 m along the 100 m transects you will measure (NOTE: measure between meters 9 and 10 along the transect): a. Percent canopy cover i. Using the pirate scope b. Percent of the canopy cover comprised of hard mast species (pirate scope) c. Percent of the canopy cover comprised of soft mast species (pirate scope) d. Percent herbaceous cover/ Percent ground cover (herbaceous cover = measurements taken in an open area, ground cover = measurements taken of the understory) i. Equal to the proportion of 1 m that contacts a herbaceous plant, measured in cm e. Herbaceous height i. Measured using the cm tape f. Percent shrub cover (NOTE: shrub = multiple woody stems) i. Equal to the proportion of 1 m is covered by a shrub g. Coarse woody debris i. Equal to the proportion of 1 m that contacts a piece of fallen woody debris greater than 10 cm in diameter, i.e. do not include twigs 6. Repeat this process every 100 m along your main transect until the entire transect has been sampled. The number of transects you do will vary with transect length. 100m 100m 100m 100m 100m 100m Transect START N Figure 1: Construction of sampling transects Figure 2: Sampling locations. Boxes indicate where tree measurements should take place (point-centered-quarter, # aspen, lowest conifer branch) – Boxes at 0 m, 50 m, and 100 m along each of the wing transects. Red dots indicate where all other measurements should take place (% canopy cover, % hard mast canopy cover, % soft mast canopy cover, % herbaceous cover or % ground cover, herbaceous height, % shrub cover, coarse woody debris) – Dots at 10 m intervals along the length of the wing transects. a b d c Figure 3: Point-centered-quarter methods. The red line represents the wing transect. The black line is an imaginary line perpendicular to the wing transect. Quartiles are labeled with lowercase letters. In each quartile you will measure the distance, indicated by the dashed blue line, to the nearest two trees, represented by the brown blobs. You will also record the species of these trees and their circumference. At these plots you will also measure the angle to the lowest branch of the 2 nearest conifers per quartile and quantify the total number of mature aspens found in all four quartiles.
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