Peripheral and uterine cytotoxicity in normal and pathologic
first trimester pregnancies
Daniela Constantinescu1*, Carmen Cozmei1, Andreea Chiriac2,
Elena Mihălceanu2, 3, Irina Dumitraşcu2, 3, Eugen Carasevici4
1. Public Health Institute, Iaşi
2. Obstetrics and Gynecology Clinical Hospital “Cuza Vodă”, Iaşi
3. University of Medicine and Pharmacy “Gr. T. Popa”, Faculty of Medicine, Obstetrics and Gynecology
4. University of Medicine and Pharmacy “Gr. T. Popa”, Faculty of Medicine, Immunology Dept, Iaşi
The maternal immune system is involved in pregnancy success or failure. Increased cytotoxic activity of NK
cells was associated with first trimester abortion, which is the most frequent complication of pregnancy. The aim of our
study was to compare peripheral and uterine cytotoxicity in normal and pathological first-trimester pregnacy, in order to
evaluate the utility of this test in monitoring high-risk pregnancies.
Material and methods: mononuclear cells were separated from peripheral blood and from tissue fragments
obtained by uterine curretage from 10 pregnant women with requested abortion and 10 women with spontaneous
abortion. Natural-killer (NK) cells percentage was detected by flow-cytometry. Separation by triple gradient Percoll
density centrifugation allowed simultaneous isolation of trophoblast cells from uterine tissue fragments. We used a
flowcytometric method of cytotoxicity evaluation which used carboxy-fluorescein-succinimidyl ester (CFSE)-labelled
K562 and autologous trophoblastic cells as targets.
Results: cytotoxic activity of uterine cells was higher than that of peripheral blood cells. Cytotoxicity was
reduced in pathological compared to normal pregnancy. Peripheral blood and uterine cytotoxicity indexes were
significantly different and were correlated only in normal pregnancies. The percentage of NK cells did nor correlate
with their cytotoxic activity.
Conclusions: increased cytotoxic activity cannot be incriminated in inducing abortion. The difference between
the cytotoxic activity of uterine and peripheral blood cells proves the compartmentalisation of uterine events.
Keywords: cytotoxicity, K562, trophoblast, CFSE, NK
* Corresponding author: Daniela Constantinescu, Str. Fundac Mircea nr 13, Iaşi, 700377, Romania
Tel.: 0232 277 289; 0722 67 12 07. E-mail: email@example.com
Institution address: Immunology and Genetics Laboratory, Emergency Clinical Hospital “Sf Spiridon”, 4th floor, Str.
Vasile Conta nr. 2-4, Iaşi, Romania
Post cardiac transplantation survival and its correlations with
the emerging complications
O.S. Cotoi1, Anca Sin1, M. Turcu2, V. C. Bacârea3, Z. Szabolcs4
1. University of Medicine and Pharmacy Tîrgu Mureş, Cellular and Molecular Biology Dept
2. University of Medicine and Pharmacy Tîrgu Mureş, Pathology Dept
3. University of Medicine and Pharmacy Tîrgu Mureş, Dept of Scientific Research Methodology
4. Department of Cardiac Surgery and Transplantation, Semmelweis University, Budapest, Hungary
Introduction. The success rate of heart transplant (HTx) is influenced by a number of factors starting with
compatibility of donor-receiver (cross-match and ABO system), ending with the effects of immunosuppressive therapy
and patient compliance. Survival and patients’ quality of life become, finally, the main criteria in evaluating the benefit
of a HTx. The aim of the study is to disclose post-transplant cardiac complications and establish correlations between
them and survival rate to a batch of 124 patients with HTx, during a period of 16 years. Material and method. Our
study monitors patients with HTx from the Cardio-Vascular Surgery and Transplant Clinic, Semmelweis University ,
Budapest . The studied lot is composed of 124 patients undergoing transplantation during a period of 16 years, when we
quantified all arisen complications and their correlations with the survival rate. Statistical data processing was
performed with the aid of Kaplan Maier survival curves, using the GraphPrism computer program. Results. In early
stages the survival curve shows a survival rate of over 95%, in the middle period a survival rate of over 83%, in the late
period a survival rate of over 60%, within 5 years and over 25% in 10 years . The longest survival rate was recorded in
the first 2 patients, who entered the17th year of their postHTx life. Discussions. Interdisciplinary collaboration in the
field of transplantation is a condition sine-qua non in patient care. Conclusions. Our study shows a very good short and
medium term survival rate and a good long-term survival rate incurages patients from the heart transplant lists. The
arising number and type of complications influence patient's quality of life and causes death. ISHLT (The International
Society for Heart and Lung Transplantation) recommends using the new classification of acute cellular rejection in all
heart transplant centers to improve reporting and comparing data in metaanalitic studies by this referring to the
obligation of monitoring all pathohistologic cases of heart transplant.
Keywords: heart transplant, monitoring, survival.
Anti-oxidized low-density lipoprotein autoantibody, IL-1α,
TNF α - cardiac risk markers
Felicia Sfrijan1*, Mădălina Golieanu2, Camelia Gurban1, Doina Drugărin3
1. Biochemistry Dept, University of Medicine and Pharmacy “Victor Babeş” Timişoara
2. Cabinet medical individual, Galaţi
3. Immunology Dept, University of Medicine and Pharmacy “Victor Babeş” Timişoara
Background: Evidence had been provided that about several interaction between inflammation and oxidized
low-density lipoprotein-oxLDL within the artery wall so that the lipid hypothesis and the response to injury hypothesis
are considered different aspects of a single pathogenic pathway.
Objective: This study was conducted to analyse the correlation between serum level of proinflammatory
cytokines IL-1α and TNF α and anti-oxidized low-density lipoprotein autoantibody (oLAB) in patients with coronary
atherosclerosis and to demonstrate implication of immune-inflammatory mechanisms in progression of atherosclerotic
Material and methods: Samples were obtained from patients with acute myocardial infarction-AMI (70),
unstable angina-UA (40), stable angina-SA (50) and from healthy subjects (40). ELISA assay was used for
quantification of serum levels of IL-1α, TNF α and oLAB.
Results: Patients with AMI and UA had significantly grown serum levels of IL-1α (p<0.05) and TNF α
(p<0.05) compared with the healthy group. The SA group differentiates from the healthy group only through grown
serum levels of TNF α (p<0.001). Significantly increased levels of oLAB appear to the AMI and UA group (p<0.05).
There is a strong correlation (r>0.6) between serum levels of proinflammatory cytokines and oLAB in the AMI group
and a variable correlation of these parameters in UA and SA a groups.
Conclusions: Increased levels of proinflammatory cytokines confirm the fact that the inflammatory reaction
appear precocious in atherosclerotic lesion and influence the progress and the stability of plaque, and the correlation
with immune markers of oxLDL may be an indicator for the prediction of the cardiac risk.
Key words: proinflammatory cytokines, anti-oxidized low-density lipoprotein autoantibody, atherosclerosis.
* Corresponding author: Felicia Sfrijan, Str. Circumvalaţiunii Nr. 47, apt.8, RO-300671 Timişoara, Romania
Tel.: 0720056353, E-mail: firstname.lastname@example.org
Institution address: Biochemistry Dept, University of Medicine and Pharmacy “Victor Babeş”, Piaţa Eftimie Murgu 2,
RO-300041 Timişoara, Romania
Selective inhibition of long chain 3-ketoacyl-Coenzyme-A-
thiolase by Trimetazidine MR in coronary heart disease
induced reduction of inflammatory syndrome and oxidative
stress in concordance with recovery of ECG and
Elena Bobescu1,2*, Mariana Rădoi1,2, Georgeta Datcu3, Zoltan Galajda4, Antoniu
Burducea2, Carmen-Daniela Neculoiu5, Roxana Bârsăşteanu5, Dorina Popa5, Valeria
Paler5, Mariana Anghel5
1. ”Transylvania” University- Faculty of Medicine, Brasov, Romania
2. Clinic County Emergency Hospital, Clinic of Cardiology, Brasov, Romania
3. University of Medicine and Pharmacy ”Gr.T.Popa”, University Hospital “Sf Spiridon”, Medical and
Cardiology Clinic I, ”C.I.Negoita”, Iasi, Romania
4. Institute of Cardiology, Cardiac Surgery Center, Medical and Health Science Center, University of
5. Clinic County Emergency Hospital, Clinic Laboratory, Brasov, Romania
The aim of this clinical study was to evaluate the efficacy of treatment with trimetazidine modified release
(MR) in addition to optimal standard medical therapy in patients with coronary heart disease - stable angina, unstable
angina and non ST elevation myocardial infarction in inflammatory syndrome and oxidative stress reduction in
concordance with recovery of ECG and echocardiographic changes.
Design: 252 patients were included in a prospective study for a period of 3 years and separated in six groups in
relation with type of coronary heart disease and addition of trimetazidine MR to optimal standard medical therapy.
Clinic, electrocardiographic and echocardiographic evaluation were performed initial, at 1, 6 months, 1, 2 and 3 years
and biologic, evaluation initial, at 1 and 6 months. Anti ox-LDL antibody titers and total antioxidant status serum level
were measured for oxidative stress evaluation, C-reactive protein serum level and fibrinogen plasma level were
evaluated as markers of inflammatory syndrome.
Results: A significant reduction in oxidative stress in terms of incidence of low total antioxidant status serum
level at 1, 6 months, incidence of high anti ox-LDL antibody serum titers at 6 months and a significant reduction in
inflammatory syndromes in terms of high C-reactive protein serum level and high fibrinogen plasma values at 6 months
of follow up were observed in patients with unstable angina and non ST elevation myocardial infarction treated with
trimetazidine added to optimal standard medical therapy. This results were in concordance with significant reduction in
incidence of ST depression on ECG at 1, 6 months, 1, 2 and 3 years and with significant improvement in
echocardiographic measured left ventricular ejection fraction at 2 and 3 years in mentioned groups of patients. Global
wall motion score index at 1, 2 and 3 years was significantly improved in all trimetazidine treated groups.
Conclusions: Selective inhibition of long chain 3-ketoacyl-Coenzyme-A-thiolase by Trimetazidine MR was
followed by a significant reduction in inflammatory syndromes and oxidative stress at 6 months of follow up in patients
with unstable angina and non ST elevation myocardial infarction. These results were in concordance with significant
reduction in incidence of ST depression on ECG and significant improvement in echocardiographic measured left
ventricular function at 3 years of follow up.
Keywords: coronary heart disease; trimetazidine MR; oxidative stress; inflammatory syndrome; ST segment
depression; left ventricular function
* Corresponding author: Elena Bobescu, ”Transilvania” University, Braşov, Romania, Faculty of Medicine, Internal
Medicine Dept; Emergency Clinical Hospital, Braşov, Romania, Cardiology Dept.
Str. Independenţei nr 1, 500157 Braşov, Romania, Tel: 0268425701, Fax:0268470318, E-mail:
Epigenetic changes in myelodysplastic syndrome and acute
myeloid leukemia: novel targets for therapy
Andrei M. Cucuianu1*, Anca Bacârea2, Mariana Paţiu1, Delia M. Dima1
1. “Ion Chiricuţă” Cancer Institute, Hematology Department, Cluj-Napoca, Romania
2. University of Medicine and Pharmacy Târgu Mureş, Clinical Laboratory Department
Epigenetic modifications, defined as DNA changes other than changed nucleotide sequence consist mainly of
hypermethylation of gene promoters and histone deacetylation. Through these mechanisms, genes, including tumor
suppressor genes can have a functionally altered expression and therefore be “silenced”. Epigenetically silenced tumor
suppressor genes are common events in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML),
usually correlating with poor prognosis. Epigenetic modifications may be useful targets for therapy in MDS and AML.
Two groups of drugs, demethylating agents (azacytidine and decitabine) and histone deacetylase inhibitors (phenyl-
butyrate, valproic acid and suberoylanilide hydroxamic acid) have recently been proven effective, either alone or in
combinations, by several clinical trials in high-risk MDS and elderly high-risk AML. These agents were able to induce
responses, even complete responses in patients known to have an extremely poor prognosis. In conclusion, epigenetic
changes are important events in MDS and AML pathogenesis and progression; targeting these mechanisms is one of the
strategies that have lately been incorporated in the therapeutic armamentarium of high-risk MDS and AML patients.
* Corresponding author: Andrei M. Cucuianu, “Ion Chiricuţă” Cancer Institute, Hematology Department, Bd. 21
Decembrie Nr 73, 400124, Cluj-Napoca, Romania
Tel: 0264-592766, Fax: 0264-598606, E-mail: email@example.com
Chronic lymphocytic leukemia developing in a patient with
polycythemia vera. Case report
Bogdana Dorcioman1*, Anca Bacârea2, Galafteon Oltean3, Smaranda Demian3,
1. Emergency Clinical Hospital Tîrgu Mureş – Medical Analysis Laboratory, Hematology Dept.
2. University of Medicine and Pharmacy Tîrgu Mureş, Dept of Laboratory-Clinical Biochemistry
3. Emergency Clinical Hospital Tîrgu Mureş, Medical Clinic I, Hematology Dept, University of Medicine
and Pharmacy Tîrgu Mureş
The paper presents a patient with eleven-years history of polycythemia vera, who developed a second
malignant disorder: B-cell chronic lymphocytic leukemia. Patient had also an intracranial tumor with secondary
neurological complications. Because of the complex pathology, the patient prognostic was unfavorable.
Key words: polycythemia vera, chronic lymphocytic leukemia, complications
* Corresponding author: Bogdana Dorcioman, str. Preot Ştefan Rusu, nr.16, Tg. Mureş, Romania.
Tel. 0722527024, E-mail: firstname.lastname@example.org
Institution address: Emergency Clinical Hospital Mureş, Medical Analysis Laboratory, Hematology Dept, str.
Gheorghe Marinescu nr. 50, Tg. Mureş, Romania.
Ferritins in iron metabolism investigation
Mariana Paţiu*, G. Laszlo, A. Cucuianu
Hematology Laboratory, Oncologic Institute ” Ion Chiricuţă”, Cluj Napoca
Ferritin is the main storage form of iron in the body. Ferritin in the serum reflects the extent of the iron stores
and is an early indicator of decreasing iron stores. Erythrocyte ferritin, reflecting the balance between iron entering and
leaving the red blood cell, can be used in monitoring therapy by bleeding in hemochromatosis patients. Mitochondrial
ferritin detection appears to be a promising new means of diagnosing sideroblastic anemia.
Key words: ferritin, serum ferritin, glycosylated ferritin, erythrocyte ferritin, mitochondrial ferritin
* Corresponding author: Mariana Paţiu, “Ion Chiricuţă” Cancer Institute, Hematology Department, Bd. 21
Decembrie Nr 73, 400124, Cluj-Napoca, Romania
The importance of tumor markers in oncology
Adina Elena Stanciu*
„Prof. Dr. Al. Trestioreanu” Cancer Institute, Bucharest, Romania
Because it's important to detect cancer early, researchers are looking for new and better tumor markers. But
as doctors have learned more about cancer, they've found that the level of a single protein or other substance in the
blood may not be the best marker for the disease. In recent years, doctors have begun to detect new tumor associated
Key words: tumor marker, neoplazic disease, sensitivity, specificity
* Corresponding author: Adina Elena Stanciu, „Prof. Dr. Al. Trestioreanu” Cancer Institute, Laboratory of
Oncogenesis and Molecular Biology, Şos. Fundeni nr. 252, sector 2, Bucharest, Romania