AKT

Document Sample
AKT Powered By Docstoc
					     How much money we need for a
          research project ?
• Financial support is a must 
  for undertaking any research 
  work.
• Unjustified request  for  fund  
  may lead to rejection of the 
  project 
• Too less of finance may lead 
  to difficulty in completion of 
  a proposed project. 
• Budget is a rational 
  approach towards 
  requirement of fund for 
  research.
              What is budget?  
• A  budget  ensures  that  sufficient  resources 
  (financial,  personnel,  and  infrastructure 
  /equipment)  are  available  to  complete  the 
  research work that is being proposed.
•  It should be based on a full analysis of research 
  project costs, and aim to get the sponsor to pay 
  for  as  many  of  those  costs  as  possible,  while 
  ensuring  that  both  the  sponsoring  agency  and 
  Institutional guidelines are followed.
                 Justification
• The budget justification narrative, required by 
  most agencies, is an important opportunity to 
  describe exactly how budget items will be used 
  to complete the study.
• A carefully constructed detailed budget increases 
  the likelihood of securing funding because it 
  documents the research team’s expertise to the 
  funding agency. An accurate budget is essential 
  to provide the necessary resources to conduct 
  the study.
 At what stage it should be done?
• Preparing a research 
  proposal budget can seem 
  like an overwhelming 
  responsibility, especially 
  for new investigators.
•  Preparing a detailed 
  budget during the 
  proposal stage can 
  minimize budget 
  management difficulties 
  once the project is funded. 
Budget of a research project
       Nonrecurring Expenditure
Refer to one time expenditure 
Equipment for Sample processing
Equipment for Storage
Instruments for Quantification
Instruments for Quantification
 Instruments for Quantification
• Spectro flurometer (8-10L): Measurement of 
  fluroescence.
• Nephlometer (2-3L) : Immuno-turbidimetry
• Chemiluminiscence (4-5L) :
• Multimode reader (12-15L) 
 Instruments for Quantification
Costly and big instruments
• Gas Chromatography (15-20L) 
• HPLC (30-50L) 
• Atomic absorption spectrophotometer (30-
  40L)
• Flowcytometer (50-60L)
• Flowcytometer  with cell sorter ( 75- 100L)
Equipments for Molecular Biology  research

• Thermo cycler              personal (2-3L) 
                                           gradient ( 5-6L)
• Gel Electrophoresis         (2-3 L)
• Gel documentation System  (5-6L)
• Real time PCR   (20-25 L)
• Gene analysis system (40L)
                                
       BUDGET FOR EQUIPMENT*
                                             Estimated cost
      Generic name of equipment   Imported /                Spare time for
S.No. with make and model                    (in foreign
                                  Indigenous                other users (in %)
                                             currency also)
       Real Time PCR (2 plex                   Rs 16.3 lakhs
 1.    with HRM)                   Imported                         50%
                                               (US$32,000)
        Inverted microscope with
                                                 Rs 5 lakhs  
 2.        digital camera and      Imported     (US$10,100) 
                                                                    50%
                computer
 3.    Deep freezer (minus 20º C) Indigenous     Rs 75,000          20%
       Liquid nitrogen containers
 4.                               Indigenous     Rs 80,000          20%
               (35 L x 2)
 Justification for the proposed equipment
• The Department  of Biochemistry,  University  College  of Medical 
  Sciences,  Delhi, where the proposed research project work will 
  be executed, has all the necessary equipments as mentioned in 
  “facilities/equipments being extended by parent institution” 
  except the above mentioned equipment.  
• A  Real  time  PCR  machine  (Q-PCR),  critical  for quantifying gene
  expression patterns, is essential for achieving the objectives. 
•  The department is initiating cell / tissue culture facility and have 
  all  the  other  equipments  such  as    Laminer  flow,  CO2  incubator 
  except  an inverted microscope with a digital camera attached to 
  a computer, for detailed image analysis. 
•  We will also require liquid nitrogen containers that will be used 
  for storing the  cell lines.
• A  dedicated  deep  freezer  will  be  essential    for  storing    tissue   
  culture  material,    temperature  sensitive  reagents  and  samples 
  for the project as the existing freezers are overloaded.  
             Recurring Expenditure
Repeating Expenditure
Ø  Manpower
Ø   Consumable
Ø   Travel
Ø   Contingency
Ø    Overhead 
•  Expenditure to be mentioned Year wise separately for  all the 
  above mentioned items depending on the experimental 
  protocol. 

  
 
                      Manpower
Research Personnel
Help in planning, execution & data collection 
• Research associate  ( 23-24 K*)                    
•  Medical officer ( 20K*)
• Senior research fellow ( 18-20 K* )
•  Junior Research  Fellow ( 16-18 K* )
• Research Assistant ( 21K *)
  * + HRA @ as admissible
            Technical Personnel
• Technical Officer/ Computer programmer                
  (29.5K*) 
• Data entry operator/ Statistician  (26.5 K*) 
• Technician/  Data entry operator/ Nurse / Field 
  worker ( 17.5*)
• Lab. Assistant/ data entry operator Jr ( 15.5K*)  
•  Office assistant/ Driver (11.9 K*)
• Lab. Attendant/ Animal attendant ( 11K*) 
* Consolidated salary
   BUDGET FOR SALARIES / WAGES
                                    BUDGET                    (In rupees)

                         1st year    2nd year     3rd year       Total
                         (m.m*)      (m.m*)       (m.m*)        (m.m.)
            Monthly
Designation
            emolument
and no. of                  –           –            –            –
            s (in
persons
            Rupees)
                         1,87,200    1,87,200    1,87,200      5,61,600
JRF (one)    15,600
                           (12)        (12)        (12)          (36)
Lab.
                                                               2,34,720
Attendant     6520      78,240 (12) 78,240 (12) 78,240 (12)
                                                                 (36)
(one)
Total           –        2,65,440    2,65,440    2,65,440      7,96,320
  Justification for the manpower requirement

(i) Junior Research Fellow (One) : The experiments and estimations 
    planned in the proposed research project will be carried out by the 
    investigators themselves / their post-graduate students. However, 
    to assist in the basic work like isolation of cells / DNA, preparation
    of reagents, sample collection and processing and data
    computation, a skilled JRF is required. Therefore, we propose to 
    recruit one JRF with M.Sc./M.Tech degree in Biochemistry/Life 
    Sciences/Medical Laboratory Technology/ Biotechnology (NET 
    qualified) with at least one year work experience in a
    Biochemistry/Molecular biology laboratory and in-hand 
    experience in molecular biology techniques; pay as per DST rules.
(ii) Laboratory attendant : The proposed project involves collection of 
    large number of human samples. Transportation of samples from 
    O.P.D. to lab, processing, storage, analysis, and disposal of
    samples, assisting tissue culture procedures like washing of
    plasticware and glassware, autoclaving and cleaning of lab
    accessories demands a full time helping hand. Therefore, we felt 
    the need of a laboratory attendant with some work experience in 
    laboratory work; consolidated pay as per DST rules. 
Consumables 
                    Consumables
To be calculated year wise as per sample size and other 
  variables. 

•   Drugs /Medicines
•   Chemicals
•   Plastic wares/ glass wares
•   Disposables
         Justification for consumables
      The budget for biological reagents and chemicals constitute a major 
      expenditure for this project.  Many of the reagents are not manufactured
      in India and have to be imported either directly or through their
      distributors.  We will require reagents for
 (i) Gas chromatography (GC) based analysis, that include HPLC grade 
      solvents, high quality  nitrogen gas and standerds and GC vials. ECD
      column used for GC can cater to approximately 300 – 400 samples.  As 
      we will process more than seven hundred samples, we will require two 
      such ECD columns
 (ii) Molecular biology  work with special requirements for Real time PCR  
      (Taqman  master mix and real time tubes), reagents / kits for DNA and 
      RNA isolation and   PCR  based reagents  such as dNTPs, restriction 
      enzymes, Taq polymerase for gene polymorphism studies 
(iii) Cell culture.  For in vitro work, two cell lines will be commercially 
      procured.  
(iv) As Real time PCR and cell culture are sensitive areas of research, we will 
      require at least two sets of dedicated autopipettes exclusively for each 
      area.    
                 Plastic wares
• Autopipette :  Routine use (3-5K)
                 For small volume (7-10K)
                 Multichannel (20K)
                 Electronic (50K)
• Other commonly required plastic wares 
  – ELISA plates, Microcentifuge tubes (2/1.5/0.5,0.2mL 
    @ 1K/1000) , Microtips (1000/100/10uL @ 1k/1000), 
    Tip boxes (1000/100/10uL @3K/12), centrifuge tubes 
    (15/50mL @ 1K/100 ), sample storage boxes 
    (2/1.5/0.5/0.2mL @ 3k/12), etc.
         BUDGET FOR CONSUMABLE MATERIALS
                                                   BUDGET                  (In Rupees)
S. No.             ITEM                 1st year    2nd year    3rd year      Total
         Taqman master mix  for 
  1.                                    40,000      40,000      30,000      1,10,000
         Real time PCR
         Taq polymerase, dNTPs,
  2.                                    35,000      35,000      20,000       90,000
         Primers, etc for PCR
         Renal Proximal tubular
  3.                                    60,000      40,000         –        1,00,000
         epithelial cell lines
         Cell culture reagents like 
  4.     FBS, RPMI, antibiotics         30,000      30,000      20,000       80,000
         mixtures, etc
         DNA / RNA isolation kits / 
  5.                                    30,000      30,000      20,000       80,000
         reagents
  6.     Glass / Plastic ware           20,000      20,000      10,000       50,000
         Automatic pipette sets 
  7.     (P1000, P200, P5-50, P2-       40,000      40,000         –         80,000
         20, P2.5)
         GC reagents (GC 
  8.                                    70,000      70,000      40,000      1,80,000
         standard, Hexane, Florisil)
  9.     GC vials, Nitrogen gas         20,000      20,000      10,000       50,000
 10.     ECD columns                    50,000      90,000         –        1,40,000
                TOTAL B**               3,95,000    4,15,000    1,50,000     9,60,000
                      F***             (US$5000)   (US$5000)   (US$1500)   (US$11,500)
                     Travel
• Expenditure for travel include :

i. Sample collection ( particularly for survey/ 
    epidemiologic/  multi centric sampling).
ii. Travel expenditure for presenting research 
    work in scientific meeting ( inland only).
iii.Attending meeting for presenting annual 
    reports to funding agency.  
                   BUDGET FOR TRAVEL

                                        BUDGET                       (In rupees)

                            1st year        2nd year     3rd year      Total

          Travel (Only 
                             10,000          20,000       20,000       50,000
         inland travel)




Justification for travel
The budget for travel will be utilized for presentation of research findings in
different scientific meetings and DST grant monitoring meetings.
              Contingency
• Expenditure under contingency include
i. Maintenance of equipment
ii. Stationary
iii.Printing material
iv.Petty expenditure
v. Postage
 BUDGET FOR OTHER COSTS /
     CONTINGENCIES

                                              (In
                         BUDGET
                                            rupees)
              1st year    2nd year 3rd year Total
 For
equipment
maintenance   10,000      20,000   20,000   50,000
and
contingency
                    Overhead
• A budgetary provision by the funding authority for 
    infrastructural expenses   to the Investigator &   
    Project executing Institute.
• Electrical fixture, AC, refrigerator, furniture, 
    telephone/ internet bill, common minor equipment, 
    contingent salary for manpower assistance, gas etc. 
• Calculated in terms of percentage of the total 
    budget excluding equipments.
    
 BUDGET ESTIMATES: SUMMARY
          Item                      BUDGET                  (In Rupees)
                        1st year     2nd year    3rd year       Total
A   Recurring
    1.                2,65,440      2,65,440    2,65,440     7,96,320
    Salaries/Wages
    2. Consumables     3,95,000      4,15,000    1,50,000     9,60,000
                      (US$5000)     (US$5000)   (US$1500)   (US$11,500)
    3. Travel           10,000       20,000      20,000       50,000
    4. Other costs      10,000       20,000      20,000       50,000
    (Maintenance of
    equipment and
    contingency)
B. Equipment           22,85,000       –            –        22,85,000
                      (US$42,100)                           (US$42,100)
    Total (A+B)        29,65,440     7,20,440    4,55,440    41,41,320
    FEC*              (US$47,100)   (US$5000)   (US$1500)   (US$53,600)
C. Overhead (20%)       5,93,088    1,44,088     91,088       8,28,264
   Grand total         35,58,528    8,64,528    5,46,528     49,69,584
 Some funded projects by various funding agency
• The effect of low glycemic composite flour on 
  diabetic and normal subjects
• Pharmacokinetic Profile of Nasal 
  Corticosteroid and Oral antiHistaminic used in 
  combination for treatment of Allergic Rhinitis 
  in rural India.
• Genotype phenotype correlation in Wilson's 
  Disease within families   
• Study of New Gemostatic Gene Polymorphisms in 
  Young Patients from North India with acute 
  Myocardial Infraction 
• Genetic, Metabolic and Hormonal Profile in 
  Obstructive Sleep Apnea Syndrome 
• Invitro inhibition of T cell Cytokines production 
  by methotrexate: Can it predict response to 
  methotrexate in patients with rheumatoid 
  arthritis 
• Gene-Environment interactions and susceptibility 
  to  breast cancer. 
         Some ICMR funded Project
• MOLECULAR BASIS OF AGE-RELATED DECLINE IN LEARNING AND 
  MEMORY ;M.S.KANUNGO 
  BANARAS HINDU UNIVERSITY, VARANASI   
• SELENOPROTEINS AND ANTIOXIDANTS IN PATHOGENESIS OF 
  ALZHEIMER'S DISEASE : P.RANI, P.S.G.COLLEGE OF TECHNOLOGY, 
  COIMBATORE   
•   PATTERNS OF FOOD ALLERGEN- SPECIFIC CYTOKINE PRODUCTION BY T 
    LYMPHOCYTE SUBSETS OF ATOPIC CHILDREN : B.P.CHATTERJEE 
    INDIAN ASSOCIATION FOR CULTIVATION OF SCIENCE ,KOLKATA 
•   A STUDY ON THE NOVEL ANALGESIC EFFECT OF INTRASPINAL 
    LOPERAMIDE IN A MURINE MODEL OF POSTSURGICAL PAIN : S.K.B.RAY, 
    ALL INDIA INSTITUTE OF MEDICAL SCIENCES ,NEW DELHI   
•     IS OXIDATIVE STRESS AND LIPID PEROXIDATION AN VIABLE MECHANISM 
    OF PERPETUATION OF VASCULAR PATHOLOGY DUE TO 
    HYPERHOMOCYSTEINEMIA?   SEEMA BHARGAVA, SIR GANGARAM 
    HOSPITAL , NEW DELHI   
                       ICMR funded project
•   EVALUATION OF PLATELET RECEPTOR POLYMORPHISM IN PATIENTS WITH 
    CORONARY ARTERY DISEASE, ENU SAXENA, ALL INDIA INSTITUTE OF 
    MEDICAL SCIENCES , NEW DELHI  
•   ESTABLISHMENT OF MOLECULAR CHARACTERIZATION OF HEMOLOGLO- 
    BINOPATHIES AND PRENATAL DIAGNOSIS OF THALASSEMIA AND SICKLE 
    CELL DISEASE AT REGIONAL CENTRES ,M.K.GHOSH,N.R.S.MEDICAL 
    COLLEGE AND HOSPITAL,KOLKATA   
•   EVALUATION OF THE BENEFICIAL EFFECTS OF TAURINE 
    SUPPLEMENTATION IN PATIENTS WITH DIABETES MELLITUS 
    ,S.SETHUPATHY,RAJAH MUTHIAH MEDICAL COLLEGE AND HOSPITAL, 
    ANNAMALAI NAGAR   
•    
 Title of the Project :
Association of Common Variants of Inflammatory
Cytokine Genes with Risk of Nephropathy in Type 2
Diabetes among Asian Indians
Objective :  
Eight single nucleotide polymorphisms (SNPs) of 
   pro-inflammatory cytokine genes (CCL2, TGFB1, 
   IL8, CCR5, and MMP9) were to be  genotyped.
Sample :
 Two independently ascertained type 2 diabetic 
   cohorts with nephropathy (n=200), without 
   nephropathy (n=200) and control ( n=200).
Methodology:
• DNA will be isolated by DNA isolation kit.
• Genotyping will be carried out by PCR- RFLP 
   using a thermocycler.
• hsCRP will be estimated by ELISA using 
   commercially available kit.
( Note:  Collaborative dept. have ELISA reader & 
   do not have a thermocycler. 50 sample DNA 
   isolation kit costs = Rs 5 K, 96 well hsCRP kit 
   costs Rs 12K)
  

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:3
posted:3/9/2014
language:English
pages:38