Non-Lethal Oil Recovery Suitable For Biocatalytic Algal Platforms R .._1_.pptx

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Non-Lethal Oil Recovery Suitable For Biocatalytic Algal Platforms R .._1_.pptx Powered By Docstoc
					       Don’t Run-Off – De-oiled, Solvent-free Algae as a Sustainable Biofertilizer
                  R. Connelly, R. Pearsall, M. Montoya, B. Morrison, K. Kaden, K. Murphy, L. Eisenberg, M. Werst, R. Hebner
                                    University of Texas at Austin, Center for Electromechanics, Austin, TX
                                                          OpenAlgae LLC, Austin, TX



         Significance of the Study                                                                                 Methods                                                                                                                     Results
                                                                                                                                                                                                                   Processed Algae Biomass Nutritional Analysis
Today, the Haber–Bosch synthetic fertilizer process
consumes more than one percent of the energy on                                                                                                                                                        Nutrient Content of Processed Algal Biomass
Earth and is responsible for feeding roughly one-            The OpenAlgae Mobile Algae Processing (MAP) unit                                                                                                                                                      %
third of the world’s population. Over time, however,                                                                                                                                                N (nitrogen)                                                   8.8
aggressive fertilizer practices have become                                                                                                                                                         P (phosphorus)                                                 5.1
                                                                                                                                                                                                    K (potassium)                                                  5.1
unsustainable and have led to a number of                              Oil Recovery                             Concentration                                       Lysing
                                                                                                                                                                                                    Ca (calcium)                                                   0.2
environmental problems and ironically, diminished
                                                                                                                                                                                                    Mg (magnesium)                                                 0.2
crop yields.
                                                                                                                                                                                                    Na (sodium)                                                    0.1
                                                                                                                                                                                                                                          N:P:K ratio (9:5:5)
The use of algae as a sustainable biofertilizer is
                                                                                                                                                                                                  The nutritional content of processed algae is similar to chemical fertilizers.
particularly appealing for many reasons. Large                                                                                                                                                    The N:P:K ratio was determined to be (9:5:5), and also contained trace
scale growth of algae is accelerating and the                                                                                                                                                     elements necessary for plant growth.
University of Texas/OpenAlgae has developed cost-
effective technologies to recover algal oils for                                                                                                                                                     Comparison of AB and CF on Tomato Yield and Height
                                                                 Source                       Harvesting                   Lysing                     Oil Separations             Clean Biomass                              50                                     50
independent sale, then use the de-oiled, solvent-              open or closed                 pH floccualtion            electric field                  membrane                        dried                                    Algae
                                                                                                                                                                                                                                  Commercial Fertilizer
free biomass as a clean and sustainable biofertilizer.                                                                                                                                                                       40                                     40

UT conducted a pilot study to evaluate the effects




                                                                                                                                                                                                         Number of Fruits




                                                                                                                                                                                                                                                                          Height in Inches
                                                         The algae were concentrated via a pH-driven flocculation process, then lysed
                                                                                                                                                                                                                                                                    30
of processed de-oiled algae on the promotion of          via exposure to an electric field of ~9 kV/cm to liberate algal lipids. The lipids                                                                                  30
                                                         were recovered with the OpenAlgae membrane technology without direct
plant growth and yield. The results showed that          contact with solvents. The de-oiled processed biomass was then dried to a                                                                                           20                                     20

the heights and yield of the biomass-conditioned         powder consistency and analyzed for nitrogen (N), phosphorus (P), potassium
                                                         (K), and protein content.
plants were consistent with, or improved, over                                                                                                                                                                               10                                     10


plants conditioned with a commercial fertilizer                                                                                                                                                                               0                                     0

applied at the same concentration. These data                Processed Algal Biofertilizer (AB) Greenhouse Study
                                                                                                                                                                                                                                       Yield              Height
                                                                                                                                                                                                  There was no significant difference between algae- and commercial fertilizer-
indicate that de-oiled algal biomass processed                                                                                                                                                    treated plants.
using OpenAlgae technologies can be used as an
organic alternative to commercial fertilizers.                                                                                                                                                         Comparison of AB and CF on Basil Yield and Height
                                                                                                                                                                                                                             60                                     60
                                                                                                                                                                                                                                  Algae
                                                                                                                                                                                                                                  Commercial Fertilizer
                                                                                                                                                                                                                             50                                     50


                  Hypotheses                                                                                                                                                                                                             *


                                                                                                                                                                                                         Weight in Grams




                                                                                                                                                                                                                                                                          Height in Inches
                                                                                                                                                                                                                             40                                     40


                                                                                                                                                                                                                             30                                     30

Using OpenAlgae clean processing technologies, de                                                                                                                                                                            20                                     20
-oiled, solvent-free algal biomass can serve as a
sustainable biofertilizer.                                                                                                                                                                                                   10                                     10


                                                                                                                                                                                                                              0                                     0
                                                                                                                                                                                                                                       Yield              Height
Plants conditioned with algal biofertilizer (AB) will                                                                                                                                             There was no significant difference between algae- and commercial fertilizer-
produce yields comparable to plants conditioned                                                                                                                                                   treated plants on plant height, but algae caused significantly more yield
                                                                                                                                                                                                  (*p < 0.05).
with a commercially available chemical fertilizer.
                                                                                                                Experimental Approach
                                                                                                                                                                                                      Comparison of AB and CF on Lettuce Yield and Height
                                                                                     Tomatoes                                    Basil                                  Lettuce                                             350                                     350

                    Methods                                                                                                                                                                                                 300
                                                                                                                                                                                                                                  Algae
                                                                                                                                                                                                                                  Commercial Fertilizer
                                                                                                                                                                                                                                                                    300
                                                                                                                                                                                                         Weight in Grams




                                                                                                                                                                                                                                                                          Height in Inches
                                                                                                                                                                                                                            250                                     250
Freshwater algae , including Chlorella sp. and
Scenedesmus sp. were processed using the                                                                                                                                                                                     30                                     30

OpenAlgae Mobile Algae Processing (MAP) unit                                                                                                                                                                                 20                                     20
(Figure 1), then dried to a powder.
                                                                                Commercial      Processed            Commercial           Processed           Commercial     Processed                                       10                                     10
                                                                                 Fertilizer        Algal              Fertilizer             Algal             Fertilizer       Algal
                                                                                                 Biomass                                   Biomass                            Biomass

The dried algae were analyzed for N:P:K and other                                                                                                                                                                             0
                                                                                                                                                                                                                                       Yield              Height
                                                                                                                                                                                                                                                                    0


for nutrient content by Texas A & M AgriLife                                                                                                                                                      There was no significant difference between algae- and commercial fertilizer-
Services.                                                                                                                                                                                         treated plants
                                                         The soil used in this study was a commercial topsoil with no added nutrients.

The analyzed dried algae (9:5:5) was applied as a
                                                         In this triplicate greenhouse trial, the effect of AB on tomato, basil, and leaf
                                                         lettuce growth and yield compared to controls supplemented with the same
                                                                                                                                                                                                                                             Conclusions
biofertilizer (12.5 kg/acre) to potted vegetables        amount of commercial fertilizer. Plant height and yields (fruits or leaf
                                                         production) were recorded over the course of 8 weeks. At the conclusion of                                                               The heights and yields promoted by algae were
and herbs. The heights and yields of the algal           the study, the bare root plants were weighed.
                                                                                                                                                                                                  comparable to, or exceeded, those produced by the
biomass-conditioned plants were monitored and
                                                                                                                                                                                                  commercial chemical fertilizer.
compared to plants conditioned with a
commercial fertilizer applied at the same
                                                                                                                                                                                                  De-oiled, solvent-free algal biomass can be utilized
concentration.
                                                                                                                                                                                                  as a biofertilizer.


                                                          THE UNIVERSITY OFTEXAS AT AUSTIN

				
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