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Curriculum Vitae - East Carolina University

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					CURRICULUM VITAE OF ALEXANDROS GEORGAKILAS
Name Surname Family status Place and Date of Birth Nationality Address Work Address : Alexandros : Georgakilas : Married with Mary Kountouraki-Georgakilas. One Child. : Athens, August 15, 1969 : Hellenic/US Permanent Resident : 737 Ashley Meadows Dr, Winterville, NC 28590 : Biology Department, East Carolina University, NC, USA Tel. : +1-252-328-5446, Fax: +1-252-328-4178 e-mail: georgakilasa@ECU.EDU

EDUCATION 1987-1992 1991-1992 1992-1993 1993-1998

: Bachelor of Science (B.Sc.) in Physics with Honors, University of Athens, Athens, Greece. : Diploma thesis with Honors “The Effects of - Irradiation on the DNA macromolecule” : Post-Graduate Studies in molecular and cellular biology, Institute of Biology, NCSR “Demokritos” : Ph.D in Biology with Honors “Effects of -Particles radiation on mammalian DNA”, Biology Department, University of Athens, Greece. (also available at : http://thesis.ekt.gr/thesisII/index.jsp?id=11182&lang=en&p=1)

PROFESSIONAL EXPERIENCE Mar 1999-Mar 2000: Post-doctoral research associate in the Institute of Biology, NCSR “DEMOKRITOS” (Supervisor Dr. E.G. Sideris). Mar 2000 - Jan 2001: Post-doctoral research associate in the Department of Physics, Health & Medical Physics Group, University of Athens, Athens, Greece (Supervisor Assoc. Prof. L. Sakelliou) and „ARETAION‟ Oncological Hospital, Athens, Greece. Feb 2001- July 2003: Post-doctoral research associate in Biology Department, Brookhaven National Laboratory, NY, USA (Supervisor: Dr. B. M. Sutherland). Jan 2004: Assistant. Professor, Biology Department, East Carolina University, NC, USA. Jan 2008:Adjunct Assist. Professor, Chemistry Department, East Carolina University, NC, USA. TEACHING EXPERIENCE 1994-1998 : Responsible for Laboratory Training Courses on “Methods of Radiation Genetics” for postgraduate students of Medical Physics, Institute of Biology, NCSR “DEMOKRITOS”. 2004: Teaching various courses in Biology Dep., East Carolina University: General Biology (BIOL 1050), Principles of Genetics (BIOL 2300), Honors Biology (BIOL 3550, BIOL 4550), Research Problems in Biology (BIOL 4504, BIOL 4514, BIOL 6514), Concepts in Cell Biology (BIOL 4230), Molecular Genetics (BIOL 5870), Thesis (BIOL 7000), Biological Effects of Radiation (RONC 7370), Introduction to Research (BISC 8830).

FELLOWSHIPS-AWARDS 1996 : ESRB 1996 Fellowship, 27th Annual Meeting of the European Society for Radiation Biology. 1997 : European Radiation Research Society Fellowship, 5th International Workshop, “Radiation Damage to DNA: Techniques, Quantitation and Mechanisms”, Windermere, UK. 1997 : NATO Fellowship, NATO ASI "DNA Damage and Repair, Oxygen Radical Effects, Cellular Protection and Biological Consequences", Antalya, Turkey 1997. 1998 : Gordon Fellowship, 1998 Gordon Conference on Radiation Chemistry, Rhode Island, USA. 2000 : Post-doctorate Fellowship of the Greek Secretariat of Technology (PENED), on the subject: “Designing and Improvement of Biological Systems for use in Radiotherapy through the MRI system (Magnetic Resonance Imaging). 2002 : Young Investigator Travel Award from Radiation Reearch Society (USA) to attend and participate the RRS Annual Meeting at Reno Nevada, 2002. 2003 : Radiation Reearch Society SIT Award (USA) to attend and participate in the 12th International Congress of Radiation Research (ICRR) on Brisbane, Australia, August 2003.

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RESEARCH EXPERIENCE 1991-1992 : Senior Student, Graduate Research under the supervision of Prof. L.H. Margaritis, Department of Biology, University of Athens , Research Director E.G. Sideris, Institute of Biology, NCSR “DEMOKRITOS”. Study of the effects of ionizing Radiation on the DNA macromolecule using UV Thermal Transition Spectrophotometry. 1992-2003 : a. Institute of Biology, NCSR “DEMOKRITOS”, Laboratory for Radiation Genetics under the supervision of Res. Dir. E.G. Sideris. Study of chromosome aberrations with conventional microscopy and Methods of Molecular Biology. b. Department of Physics, University of Athens, Laboratory of Medical Physics under the supervision of Ass. Prof. L. Sakelliou and Dr. G. Zarris. Dosimetry of a 60Co using TLD measurements and of an 41Am surface source using CR-39 measurements. Modeling of γ-radiation Induced DNA damage using Monte Carlo simulation and Fortran programming. c. Institute of Nuclear Physics, NCSR “DEMOKRITOS”, under the supervision of Res. Dir. Physicist C.A. Kalfas. Modeling of DNA thermal transition using methods of arithmetical analysis and Fortran programming. d. Department of Physics, National Polytechnic School of Athens, under the supervision of Assoc. Prof. A. Konsta. Study of radiation-induced DNA damage using dielectric relaxation spectroscopy (DRS). e. Institute of Biology, NCSR “DEMOKRITOS”, Laboratory for Radiation and Molecular Genetics, under the supervision of Assoc. Res. Biol. V. Sophianopoulou. Study of the Radiation induced DNA Damage using methods of Molecular Biology (Pulsed Field Gel Electrophoresis, Southern Blotting, Hybridizations). f. Department of Cell Biology and Biophysics, University of Athens, under the supervision of Prof. E.N. Moudrianakis and Prof L.H. Margaritis. Mammalian DNA isolation and purification. Optimization of DNA fragmentation using different techniques. g. Department of Physics,- Health & Medical Physics Group, University of Athens, Athens, Greece, University of Athens and „ARETAION‟ Hospital, Athens, Greece, Magnetic Resonance Imaging studies on biological materials simulating human tissues. h. Biology Deparment, Brookhaven National Laboratory. NY, USA, Detection of closely spaced DNA damages (clustered DNA damages) in isolated DNA and in human cells, using different repair enzymes or chemical agents. Detection of apoptosis using fluorescence microscopy. PARTICIPATION IN INTERNATIONAL MEETINGS 1992 : EBSA International Workshop on Water-Biomolecule Interactions, Palermo, Italy. 1994 : 1st Mediterranean Congress on Radiation Protection, Athens. 1994 : IEEE Trans. Dielectr. Electr. Insul. Society ISE 8, Paris, France. 1996 : 27th Annual Meeting of the European Society for Radiation Biology 1996 : Erasmus Intensive Course on “Transduction Phenomena and Sensors” 29 June- 3 July Pisa, Italy. 1996 : IEEE Trans. Dielectr. Electr. Insul. Society ISE 9, Sanghai, China. 1997 : 5th International Workshop, “Radiation Damage to DNA: Techniques, Quantitation and Mechanisms”, Windermere, UK . 1997 : 1st Biological Meeting of Balkan Countries, Thessaloniki, Greece. 1997 : NATO ASI “DNA Damage and Repair: Oxygen Radical Effects, Cellular Protection and Biological Consequences”, Antalya, Turkey 1998 : 1998 Gordon Research Conference on Radiation Chemistry 1998 : Dielectrica98 “Relaxation in Dielectrics”. Dielectric Investigation of Biological and Bio-Compatible Systems by A.G. Georgakilas, A.A. Konsta and E.G. Sideris (Invited Speech). 1998 :Third TESLA Workshop, Radiation Research with Ion Beams, April 6-7, 1998, Beograd, Yugoslavia 1999 : VI International Conference on Medical Physics, Patras, Greece. 2000 : 6th International Conference on Dielectric and Related Phenomena, DRP 2000, Spala, Poland 6-10 September 2000 (Invited Speech). 2001 2001 2002 : DS 2001, 1st International Conference on Dielectric Spectroscopy in Physical, Chemical and Biological Applications, Jerusalem, Israel . : 48th Annual Meeting of the Radiation Research Society, April 21-25, San Juan, Puerto Rico, USA. : 49th Annual Meeting of the Radiation Research Society, April 21-25, Reno , Nevada, USA.

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:12th International Congress of Radiation Research (ICRR) August 18-23, 2003, Brisbane, Australia.

NATIONAL MEETINGS Numerous Presentations in meetings of Greek and US Scientific Societies. BIBLIOGRAPHY I. PUBLICATIONS IN PEER REVIEWED SCIENTIFIC JOURNALS 1. E.G. Sideris, A.G. Georgakilas, C.A. Kalfas and Anagnostopoulou-Konsta A. (1994) “Thermally stimulated electric changes during the helix to coil transition of irradiated DNA‟‟, IEEE Dielectric and Electric Insulation Society, ISE 8, 857-862. 2. C.A. Kalfas, G.K. Loukakis, A.G. Georgakilas and E.G. Sideris (1996) “Flexibility and Thermal Denaturation (Melting) of Irradiated DNA”, J. Biol. Sys. 4, 405-423. 3. A.G. Georgakilas, K.S. Haveles, A. Konsta and E.G. Sideris (1996) “Dielectric Changes Precede Hydrogen Bond Breakage During Helix to Coil Transition of Deoxyribonucleic Acid”, IEEE Dielectric and Electric Insulation Society, ISE 10, 747-752. 4. A. G. Georgakilas, V. Sophianopoulou and E. G. Sideris (1997) "Enhanced DNA Stability as a Result of Low Doses of - and -Irradiation", Radioprotection 32: C1-101-102. 5. A. G. Georgakilas, K. S. Haveles, L. C. Margaritis, L. Sakelliou and E. G. Sideris (1997) "Conformational Changes Induced on the DNA by Exposure to Low Doses of - and -Irradiation”, Radiation Research 148, 503-504. 6. K. S. Haveles, A. G. Georgakilas, V. Sophianopoulou and E. G. Sideris (1997) “Effects of Tris on the DNA Structure and the Problem of DSB‟s Induction by Ionizing Radiation”, Radiation Research 148, 500-501. 7. A.G. Georgakilas, A.A. Konsta, K.S. Haveles and E.G. Sideris (1998) “Dielectric Study of the Double Helix to Single Coil Transition of the DNA Macromolecule”, IEEE Trans. Dielectrics El., 5, 26-32. 8. A. G. Georgakilas, L. Sakelliou, L. H. Margaritis, E. G. Sideris and V. Sophianopoulou (1998) "Effects of Rays on DNA Stability and Size", Radiation. Research 150, 488-491. 9. G. Zarris, A. G. Georgakilas, L. Sakelliou, K. Sarigiannis and E. G. Sideris (1998) - and -Irradiation of aqueous DNA Solutions‟‟, Radiat. Measurements 29, 611-617. 10. A. G. Georgakilas, E. G. Sideris, L. Sakelliou and C.A. Kalfas (1999) “Low Doses of - and -Radiation Enhance DNA Thermal Stability”, Biophysical Chemistry 80, 103-118. 11. A.G. Georgakilas, K.S. Haveles, V. Sophianopoulou, G. Zarris, L. Sakelliou and E.G. Sideris (1999). “- and -Radiation Induced Changes on the Stability and Size of Mammalian DNA in Solutions” Physica Medica XV, 198-200. 12. K. S. Haveles, A. G. Georgakilas, E. G. Sideris and V. Sophianopoulou (2000) “Effects of Radical Scavengers on Radiation Induced Double Strand Breaks”, Intern. J. Radiat. Biol. 76, 51-9. 13. A.G. Georgakilas, K.S. Haveles, V. Sophianopoulou, L. Sakelliou, G. Zarris and E.G. Sideris (2000) “Particle-Induced Changes on the Stability and Size of DNA”, Radiation. Research 153, 258-262. 14. A.G. Georgakilas, A.A. Konsta , L. Sakelliou and E.G. Sideris (2001) “Dielectric and UV Spectrophotometric Study of Physicochemical Effects of Ionizing Radiations on Mammalian Macromolecular DNA”, IEEE Transactions on Dielectrics and EI, 8, 549--554. 15. E.G. Sideris, A.G. Georgakilas, K.S. Haveles, A.A. Konsta, V.Sophianopoulou, E.E.Visvardis (2001) “The „Balkan Syndrome‟ of depleted uranium-effected leukemia: facts and fears”, Journal of the Balkan Union of Oncology, 6, 231-235. 16. A. Konsta, E-E. Visvardis, K.S. Haveles, A.G. Georgakilas and E.G. Sideris (2002) “Detecting radiationinduced DNA damage: From changes in dielectric properties to programmed cell death”. Journal of nonCrystalline Solids, 305, 295-302. 17. A.G. Georgakilas, P. V. Bennett and B. M. Sutherland (2002) “ High efficiency detection of bistranded abasic clusters in -irradiated T7 DNA by putrescine”. Nucleic Acids Research, 30, 2800-2808. 18. Sutherland, B.M., Bennett, P.V., Georgakilas, A.G., and Sutherland, J.C. (2003) “Evaluation of Number Average length Analysis in Quantifying Double Strand Breaks in Genomic DNAs”. Biochemistry, 42, 33753384. 19. B.M. Sutherland, A.G. Georgakilas, P.V. Bennett, J. Laval and J.C. Sutherland (2003) “Quantifying Clustered DNA Damage Induction and Repair by Gel Electrophoresis, Electronic Imaging and Number Average Length Analysis”. Mutat. Res. (Review) 531, 93-107. 20. A.G. Georgakilas, P. V. Bennett and B. M. Sutherland (2004) “Processing of Bistranded Abasic DNA Clustered Damages in γ-Irradiated Human Hematopoietic Cells”. Nucleic Acids Res. 32, 5609-5620.

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Gollapalle, E., Wong, R., Adetolu, R., Tsao, D., Francisco, D., Sigounas, G. and Georgakilas, A.G. (2007) “Detection of oxidative clustered DNA lesions in X-irradiated mouse skin tissues and human MCF-7 breast cancer cells”. Radiat. Res. 167, 207-216. D. Tsao, P. Kalogerinis, I. Tabrizi, M. Dingfelder, R.D. Stewart and A.G. Georgakilas (2007) “Induction and processing of oxidative clustered DNA lesions and double strand breaks induced by high-LET 56Fe space radiation in human monocytes”. Radiat. Res. 168, 87-97. Holt, S. M.; Georgakilas, A. G. Detection of complex DNA damage in γ-irradiated acute lymphoblastic leukemia pre-B NALM-6 cells. Radiat. Res. 168:527-534; 2007. Lally, B. E.; Geiger, G. A.; Kridel, S.; Arcury-Quandt, A. E.; Robbins, M. E.; Kock, N. E.; Wheeler, K.; Peddi, P.; Georgakilas, A. G.; Kao, G. D.; Koumenis, C. Identification and biological evaluation of a novel and potent small molecule radiation sensitizer via an unbiased screen of a chemical library. Cancer Res. 67:8791-8799; 2007. Francisco, D. C.; Peddi, P.; Hair, J. M.; Flood, B. A.; Cecil, A. M.; Kalogerinis, P. T.; Sigounas, G.; Georgakilas, A. G. Induction and processing of complex DNA damage in human breast cancer cells MCF7 and non-malignant MCF-10A cells. Free Radic. Biol. Med. 44:558-569; 2008. Georgakilas, A. G. Processing of DNA damage clusters in human cells: Current status of knowledge Mol. Biosyst. 4:30-35; 2008 (Review). Poulos, J. E.; Georgakilas, A. G.; Kalogerinis, P. T.; Daignaut, T.; Lujan, G. A Patient with Granular Cell Tumor of the Colon. Practical Gastroenterology In press; 2008.

II. PUBLICATIONS IN BOOKS AND PROCEEDINGS FROM INTERNATIONAL MEETINGS 1. E.G. Sideris, G. Zarris, A. Anagnostopoulou-Konsta, A.G. Georgakilas and C.A. Kalfas (1993) “DNA-Water free Radicals interaction and the thermostability of the DNA molecule”, SIF Conference Proceedings 43, p. 219-222. 2. A.G. Georgakilas, G. Zarris, E.G. Sideris and L. Sakelliou (1994) “High LET irradiation of aqueous DNA solutions: II. Experimental results‟‟, Proceedings of the 1st Mediterranean Congress on Radiation Protection, 5th-7th April, Athens, Greece 1994, p.151-154. 3. G. Zarris, A. Angelopoulos, A.G. Georgakilas, P. Karaiskos, E. Pappas, A. Perris, L. Sakelliou and E.G. Sideris “High LET irradiation of aqueous DNA solutions: I. Dosimetry”, Proceedings of the 1st Mediterranean Congress on Radiation Protection, 5th-7th April, Athens, Greece 1994, p. 82-86. 4. K.S. Haveles, A.G. Georgakilas, T. Katsorchis, and E.G Sideris (1996) “Free radical scavengers effect on the helix to coil transition of the DNA macromolecule”, Molecular Properties and Chemisty of Biological Systems, Ioannina, Greece, Ed. Dell’ Orso, p.203-208. 5. A.G. Georgakilas, L.C. Margaritis, L. Sakelliou and E.G. Sideris (1997). “- and -Radiation Induced Changes on the Stability and Dielectric Properties of Mammalian Macromolecular DNA”, Proceedings of the 1st Biological Meeting of Balkan Countries,15-18 May Thessaloniki, Greece, p. 121. 6. K.S. Haveles, A.G. Georgakilas, Th. Katsorchis and E.G. Sideris (1997) “Role of Tris on the Stability of the Irradiated DNA Aqueous Solutions”, Proceedings of the 1st Biological Meeting of Balkan Countries,15-18 May Thessaloniki, Greece, p. 149. 7. A.G. Georgakilas, A.A. Konsta, K.S. Haveles, A. Kanapitsas and E.G. Sideris (1997) “Comparative Study of the DNA helix to coil thermal transition by dielectric spectroscopy and thermal transition spectrophotometry”, Spectroscopy of Biological Molecules: Modern Trends, Kluver Academic Editions, p. 255-256. 8. A.G. Georgakilas, L.C. Margaritis, T. Katshorhis, L. Sakelliou and E.G. Sideris (1997) "Influence of - and Radiation Induced Nicks on the Physical Properties of Calf Thymus DNA" Proceedings of the NATO ASI Conference in Antalya, Turkey 1997, p.115-116. 9. A. G. Georgakilas, _. S. Haveles, L. H. Margaritis, E. G. Sideris and V. Sophianopoulou (1998) - and radiation induced DNA-DNA interactions in solution" Proceedings of the 46th Annual Meeting of the Radiation Research Society and the 17 th Annual Meeting of the North American Hyperthermia Society, Louisville, Kentucky, p. 192, P21-405. 10. K. S. Haveles, A. G. Georgakilas, L. H. Margaritis, E. G. Sideris and V. Sophianopoulou (1998) "Radical Scavengers effects on mobility and thermostability of DNA molecules. Proceedings of the 46th Annual Meeting of the Radiation Research Society and the 17th Annual Meeting of the North American Hyperthermia Society, Louisville, Kentucky, p. 192, P21-406.

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A.G. Georgakilas, A.A. Konsta and E.G. Sideris (2000) “Dielectric and UV Spectrophotometric study of physicochemical effects of ionizing radiation on mammalian macromolecular DNA”. Proceedings of 6 th International Conference on Dielectric and Related Phenomena, DRP 2000, Spala, Poland, 6-10 September 2000. K.S. Haveles, E. Visvardis, A.G. Georgakilas, V.Sophianopoulou and E.G Sideris (2001) “Distribution of DNA strand breaks in -irradiated human lymphocytes”, Proceedings 48th Annual Meeting of the Radiation Research Society, p. 102. S. Voyiatzi, A. Angelopoulos, A. G. Georgakilas, M. Kamariotaki, I. Seimenis and L. Vlachos (2001) “MRI of immobilized protein solutions: signal dependence on magnetization transfer contrast (MTC) prepulses”, Proceedings of International Conference on Imaging Technologies in Biomedical Sciences, 20-24 May 2001, Milos Island, Greece, p. 72. B.M. Sutherland, P.V.Bennett, N. Cintron, A.G.Georgakilas, P.Guida, M.Hada, S.Paul, H.Schenk, J.C.Sutherland, D.Monteleone, J.Trunk and J. Laval (2001) “Clustered DNA damages”, DOE/NASA Radiation Investigator’s Workshop, 27-30 June 2001, Washington, DC. A.G. Georgakilas, P.V. Bennett and B.M. Sutherland (2002). “Detecting Clustered DNA Abasic sites by Use of Polyamines”, Proceedings 49th Annual Meeting of the Radiation Research Society, Reno, Nevada, USA, p. 14. EE Visvardis, KS Haveles, AG Georgakilas, EG Sideris, V Sophianopoulou (2003). Patterns of DNA DSBs, SSBs and alkali-labile sites induction and repair in γ-irradiated human lymphocytes using alkaline and neutral PFGE. EU-US Workshop on Molecular signatures of DNA damage induced stress responses. September 26-30, Cortona, Italy. B.M. Sutherland, P.V. Bennett , A.G. Georgakilas and M. Hada (2003) “Bistranded DNA Damage Clusters Induced by Low LET Radiation and Heavy Charged Particles: Formation and Repair” 14 th Annual NASA Space Radiation Investigator’s Workshop, April 27-30, 2003, Houston, Texas, USA, p.12. A.G. Georgakilas, P.V. Bennett and B.M. Sutherland (2003) “ Delayed Repair of Clustered Abasic-DNA Damage in γ-Irradiated Human Cells”, Proceedings of the 12th International Congress of Radiation Research (ICRR) August 18-23, 2003, Brisbane, Australia,.p. 35-36. E.E Visvardis, K.S Haveles, A.G Georgakilas, E.G Sideris, V. Sophianopoulou (2003). Patterns of DNA DSBs, SSBs and alkali-labile sites induction and repair in γ-irradiated human lymphocytes using alkaline and neutral PFGE. EU-US Workshop on Molecular signatures of DNA damage induced stress responses. September 26-30, Cortona, Italy. S.M. Holt, D.C. Francisco, M. Hada and A.G. Georgakilas (2005) “Detection of oxidative clustered DNA lesions in human cancerous cell lines”. Proceedings 52th Annual Meeting of the Radiation Research Society, Denver, Colorado, USA, p. 65. D. Tsao, I. Tabrizi, M. Dingfelder, R.D. Stewart and A.G. Georgakilas (2006) “Induction and processing of oxidative clustered DNA lesions and double strand breaks induced by high-LET 56Fe space radiation in human monocytes. Proceedings 53th Annual Meeting of the Radiation Research Society, Philadelphia, Pennsylvania, Nov 5-8, USA, p. 53. S.M. Holt, J.L. Scemama and A.G. Georgakilas (2006) Processing of oxidative clustered DNA lesions (OCDL) in the MSH2 deficient acute lymphoblastic leukemia human cell line NALM-6. Proceedings 53th Annual Meeting of the Radiation Research Society, Philadelphia, Pennsylvania, Nov 5-8, USA, p. 54. B. Flood, D.C. Francisco, P. Peddi and A. G. Georgakilas (2007) “Processing of oxidative clustered DNA lesions in human breast cancer MCF-7 and non-malignant breast MCF-10A cells”. Proceedings of the North Carolina Academy of Sciences 104th Annual Meeting, Greenville, NC, Mar 30- Apr 1, 2007, P3, p.5 R. Hudgins, C. Cooke, G.W. Kalmus, A. G. Georgakilas and G. Sigounas (2007) “The effect of erythropoietin on prostate cancer cells expressing erythropoietin and/or its receptor”. Proceedings of the North Carolina Academy of Sciences 104 th Annual Meeting, Greenville, NC, Mar 30- Apr 1, 2007, P27, p.7. P. Peddi1, D.C. Francisco1, J. M. Hair1, P.K. Katikaneni, A.M. Cecil1, B.A. Flood1, G. Sigounas and A. G. Georgakilas (2007) “Role of DNA-PKcs in the processing of double stranded breaks and oxidative clustered DNA lesions in the human breast cancer cell line MCF-7”. Proceedings of the North Carolina Academy of Sciences 104th Annual Meeting, Greenville, NC, Mar 30- Apr 1, 2007, p.13. Peddi, P.; Francisco, D. C.; Flood, B. A.; Sigounas, G.; Georgakilas, A. G. Accumulation of oxidative clustered DNA lesions (OCDLs) in the human breast cancer cells MCF-7. Proceedings 53th Annual Meeting of the Radiation Research Society. Philadelphia, Pennsylvania, Nov 5-8, USA; 2007: 53. Hair, J. M.; Peddi, P.; Francisco, D.; Flood, B.; Cecil, A.; Georgakilas, A. G. (PS3085) Induction and processing of oxidative clustered DNA lesions in the human breast cancer lines MCF-7, MCF-10A and

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HCC1937. In: Society, R. R., ed. International Congress of Radiation Research San Francisco, CA 2007: 169-170.

REVIEWING Reviewer for Radiation Research, Phys. Med. Biol., DNA Repair journals. Reviewer for Belk/Borden: Science for life, 2nd Edition Biology Book. Reviewer for Cain et al.: Discover Biology, 3rd Edition, W.W. Norton & Co. Reviewer for the 6nd Edition, Berg/Stryer, Biochemistry Book, Freeman. Reviewer for the 5th Edition of Klug and Cumming‟s: Essentials of Genetics 5th Ed. . INVITED LECTURES 1. Biology Department, East Carolina University, North Carolina, USA with subject: “Clustered abasic-DNA damage: A challenge for the human cells” Hosted by Prof. R. Newton (Chair), May 2003. 2. Biology Department, National University of Greece, Athens, Greece with subject: “Repair of Clustered DNA Damages by the human cell”. Hosted by Prog. L. H. Margaritis (Chair), July 2003. 3. Biology Institute, Nuclear Center for Scientific Research „Demokritos‟, Athens, Greece, July 2003 with subject: “Detection and analysis of clustered DNA Damage”. 4. Institute of Radioisotopes and Radiodiagnostic products, Nuclear Center for Scientific Research „Demokritos, Athens, Greece, July of 2003 “Induction of oxidative clustered DNA lesions by low doses of ionizing radiation”. 5. National Hellenic Research Foundation, Institute of Biological Research and Biotechnology, Athens, Greece “Role of clustered DNA damage in mutagenesis and carcinogenesis”, May 2004. 6. Department of Materials Science and Engineering, Penn State University, USA with subject “Using Biophysical, Biochemical and Molecular Techniques in the Detection of Oxidative DNA Damage”. 7. Department of Biochemistry, ECU School of Medicine, November 28, 2005 “Detection of oxidative clustered DNA lesions in human cells and mammalian tissues”. 8. Department of Anatomy and Cell Biology, ECU Brody School of Medicine, October 2006 “Detection of oxidative clustered DNA lesions in human breast cancer cells and mammalian tissues” 9. Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, February 2007 “Processing of complex DNA damage in human breast cancer cells and tissues”. Invitation by Dr. William Bonner. 10. Department of Chemistry, ECU, March 30, 2007 “Detection of oxidative DNA lesions in cancer: A fact or a myth?”. Invitation by Dr. A. Morehead. 11. School of Health Sciences, Purdue University, October 2007 “Detection of Oxidative Clustered DNA Lesions in Human Cells and Tissues”. Invitation by Dr. R.D. Stewart, Director of Undergraduate Program in Radiological Health. 12. Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, February 2007 “Processing of complex DNA damage in human breast cancer cells and tissues”. Invitation by Dr. William Bonner, Dir.

PARTICIPATION AS CO-RESEARCHER IN EU INTERNATIONAL RESEARCH PROGRAMMES 1996-1999: Participation in the EEC/Euratom financed program under the title: „Experimental Data for the induction of cancer by radiation of different qualities‟ (FI4P-CT-950011, 1995-1999, 60000 Euros) (Senior Scientist Dr. E.G. Sideris). 2000-2001: Participation as CO-PI in FIS5-99-00079 Grant “Induction Repair and Biological Consequences of DNA Damage Caused by Radiation of Various Qualities” (2000-2004, 60000 Euros). Both grants were awarded by the XII General Direction (General Direction of Research, Technology & Development) of the EU. 2001-2003: Participation in a NIH (National Institute of Health) USA funded research program entitled: ‘DNA Damage Clusters: Repair in Mammalian Cells’ (Supervisor: Senior Scientist Dr. B.M. Sutherland).

PERSONAL FUNDING OF MY RESEARCH

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2005: “Is Mercury efficient in inducing Oxidative Clustered DNA Damage in Human Hematopoietic Cells?”, Amount: ~17,000. East Carolina University/Creative Activity Award Program. PI. Awarded.
SPECIALIZATION 1. Detection and analysis of ionizing radiation-induced DNA damage, repair and apoptosis applying different techniques of biochemistry, molecular and cellular biology such as: a. Different agarose gel electrophoreses including DNA laddering for the detection of apoptotic DNA b. Different staining methods of microscopic visualization of DNA and human cells c. Southern Hybridization d. Thermal Transition Spectrophotometry e. Dielectric Studies of biomolecules and human cells with Impedance Analyzer f.. Use of different repair enzymes and electrophoresis strategies to detect DNA clustered damage 2. Extraction and purification of mammalian DNA 3. Chromosome aberrations and conventional Microscopy 4. Dosimetry of -radiation sources and -radiation sources specially designed for low exposure rates and irradiation of DNA solutions and human cells. 5. Modeling of Radiation Induced DNA Damage and UV Thermal Transition of DNA Macromolecules with Fortran programming 6. Cell cultures and handling of different cell lines: hamster V79, CHO cells, human cells (monocytes and lymphoblasts) and skin fibroblasts. Isolation of human lymphocytes. 7. Different kind of Gel Electrophoreses including Pulsed Field Gel Electrophoresis (PFGE). 8. Comet assay under neutral and alkaline conditions. 9. Participation in Alternating Gradient Synchrotron (AGS) experiments of irradiation of biological samples with different high LET radiations, at Brookhaven National Laboratory, NY, USA. 10. Clonogenic survival studies.

OTHER ACTIVITIES-MEMBERSHIP IN INTERNATIONAL SCIENTIFIC SOCIETIES Member of the Sigma Xi (Scientific Research Society), Member of the Radiation Research Society since 1998, Member of Federation of American Societies for Experimental Biology (FASEB) since 2001. ARMY SERVICE 1998-2000 : Served as an officer in the Greek Army.

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