King Lab Yearly Safety Review
by Cammie Haase-Pettingell
for March 2005
Disclaimer-This is Jonathan King’s lab in Biology Dept.
yearly safety review and not to be substituted for an initial
Any questions should be
directed to Cammie 3-3545
Points be covered
Emergency Action Plan
Biologicals, Blood Borne Pathogens, Infectious Agents
n Ensure that the lab is conducting in a safe manor
w This is by the PI or by a representative the
n To conduct incoming safety orientation
n Go to safety meetings
n Update the lab on new policy
n To follow the above policy
No Eating in lab
n Can eat in
w Tea room
w Computer room ,
w hall and seminar room
No smoking except outside
No mouth pipeting
Close the lab doors
Pull the fire red fire alarm
n Located by Stairwells , Elevators
Leave by stairwells
Get to a phone- dial x100 report fire
n Don’t hang up till they have hung up
Not responsible to fight a fire
Unknown fire such as strange smell
If in doubt call x 100
Pull fire alarm
Call John Fuccilo to ask he know anything
n Ex . melted stuff in autoclave
The equipment halls can’t hear the alarm
n As exit ensure people know about the alarm
If can - put experiments to rest- not required you safety is
n So firefighters
w ie do not stumble upon a active electrophoresis.
n Just in case not back for a while may not be ruined
Evacuate by stairwell -all are fire stairwells
Meet by the concrete post by bld 66
n even if you think I saw you Check in so we know no one is stuck
in the building
w Then go off for what ever.
Some one needs to go to the command post say we are all out
n by the door toward Main St. -
MSDS see handout
Chemical safety web training
n 68-340D behind door
n 68-322 under sink
Simple Laminar Flow Hood
Ensure the back “slot” in back is clear
n have items on lifts so it is not blocked
Simple test for flow
n to tape Kimwipe
If malfunctioning- inform fixit Zone -A
Rooms air flow and temperature is dependant on
n Clear Area
n Get on appropriate safety gear
n Determine the Scope
n Use the appropriated cleaner
n Dispose correctly
Look at the Red flip chart by phone
w Evacuate area- put sign up
w Done appropriate safety - lab coat, eye protection,
w Determine the necessary sorbant,
w For acid base or solvent.
w Pour around the outside of the spill - mix in
w I use the dust pan and broom sweep up.
w Wipe up residue
w Dispose of as waste
Chemicals and Medical Emergency
Do not block body showers
Eye wash put injured eye down flush for 15 min.
Eyewash or distilled wash with tubing
n Needs to be tempered water
Cut and injury- should go to Medical
n can be documented and treated properly
n Ensure you are not putting you self in danger
n If can rise -call EMT X100
EMT will be concern that they are not in danger
n try to figure what happened
Emergency number X100
If working alone at night
n inform some one on the Hall
n Or call campus police 3-1212
Safety is important to everyone.
Waste needs a red tag
Must be closed when not in use.
Most is in electrophoresis room
n Silver- silver stain
w Gel drying fix. , Transfer solution
n Ethidium bromide -
w Liquid and tips
w Agarose in the dark room
Once full date and should be picked up immediately by law 2
n If can call don’t date till wedded in link on King links page
n Biological or not chemical waste-gray bin
w If it is sharp from a biological lab it can not
be just thrown out.
n Chemical waste- with red tags
At the Bench
n Need to have covers and be covered when not in
Radiation ext. 3-2180
We use 14C and 35S to label rapidly proteins
Disposal designated by 1/2 life
n 20-120day 1/2 life - S- 88 day 1/2 life
n Over 120 day 1/2 life C = 5730 yr 1/2 life
w Type- dry (paper gloves), hi level aqueous, sharps/epi.
n is stored in the locked refrig and freezer 68-346D
Most experiment are preformed in the 68-346D.
Geiger counters in each side of the lab.
n Pancake probes more sensitive
n Ensure the battery is off when done.
Clear the area- block off
Ensure they you have lab coat, safety glasses, double gloves,
Monitor the area with Geiger counter
Mark the area- where there are counts
One does not inactivate the radioactive just clean off the
n Good agents -Bleach, Radiact wash, Am4OH
n Clean till no signal
RPO will do wipe test
If still signal cover till decayed.
BL1- E. Coli
low BL2 - Salmonella typhurminium LT2
n All bacteria needs to be in activated by before
disposal or going to the kitchen
w Chemical, 10% bleach, 1% Westcodyne, Lysol
n Plates go into the bucket autoclaved in big
autoclave after kitchen hours.
Block the area
Let aerosol settle
Clean up by pour/spray disinfectant on glassware
n with dusty pan
Put paper towel on it and pour/spray disinfectant
Dispose of in the waste.
Look for the new biological spill kits
Decontaminating the Bench
70% ethanol is a better agent to decontaminate the
bench than 100% ethanol
n 70% is better at denaturing the outer
membrane and subsequently lysing the cell
n 100 % will only causes effects the on the outer
membrane but not lysis.
Any voltage should be treated as if it is dangerous.
n AC current is the most dangerous.
Running a PAGE
n Start up a gel by connecting the black lead first as a ground and
then the red lead.
w It should be noted that high power leads should be used when
running a sequencing gel with high voltage.
n Turn off a power supply by turning down the voltage source, then
turn off the power.
w After turning off power, disconnect the red (positive) lead
If the hazard light is flashing on the power supply, turn it off and
determine what the problem is and fix it, or have it fixed.
If someone is unconscious, call x100 which is the emergency phone
number at MIT.
Chemical Hygiene Plan
OSHA right to know
MSDS know how to read
SOP- Standard Operating Procedures
n a written procedure for use of every chemical
Safety is everyone responsibility
Everyone will be responsible for the level 1 check
list which I email the pdf
n keep check off for a month
n Cammie and Shannon are the safety Reps to do
an over view
ensure all unlabeled liquid containing item have a
label can be cryptic