University of Sheffield

							University of Sheffield Department of Engineering Materials

6 August 2002

CoSHH Assessment – Experimental
CoSHH Ref No: 02/069/JWH Lab where procedure Will be carried out: G4 Describe Procedure: Making up keratinocyte and fibroblast culture media List ALL Substances and Quantities Used (including product):
Sodium hydrogen carbonate (17mls) Insulin (2.5mls) Transferrin (0.5mls) Hydrocortisone (0.080mls) May be harmful if ingested. Biohazard, may be capable of transmitting infectious agents. Harmful if in contact with skin. Possible risk of harm to the unborn child. May be irritating to eyes, respiratory system and skin May be irritating to eyes, respiratory system and skin. Biohazard. May cause human disease. Harmful in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Contact with acids liberates a very toxic gas. Due to low % of toxic material (<0.1%) material is low risk. May be harmful if ingested Due to low % of toxic material (<0.1%) material is low risk. May be harmful if ingested May be harmful if inhaled, ingested or in contact with skin. May be harmful if inhaled or ingested. May be harmful if inhaled, ingested or in contact with skin. May be harmful if inhaled, ingested or in contact with skin. May be harmful if ingested. May be harmful if inhaled, ingested or in contact with skin. May cause irritation in heart and C.N.S.

Hazards Identified:

May be harmful if inhaled, ingested or in contact with skin.

Epidermal Growth Factor (0.025ml) Cholera Toxin (0.5mls)

Nutrient Mixture F-12 Dulbeccos Modified Eagles Medium

Fungizone (2.5 mls) Adenine (2mls) Glutamine (5mls) Penicillin (5mls) Foetal Bovine Serum (50mls) Tri iodo-L- Thyronine

Particular Control/safety Measures to be adopted:
(include where and how substances are stored)
Sodium hydrogen carbonate : Stored in a sealed container on the laboratory shelf. Wear lab coat and gloves. Insulin : Stored in a sealed container in the fridge. Wear lab coat and gloves and safety specs. Transferrin : Stored in a sealed container in the freezer. Only handle in a Class II cabinet. Wear lab coat and gloves. Hydrocortisone : Stored in a sealed container in the freezer. Only handle in a Class II cabinet. Wear lab coat and gloves. Epidermal Growth Factor : As above Cholera Toxin : Stored in a sealed container in the freezer. Only handle in a Class II cabinet. Wear lab coat and gloves. Keep away from acids. Nutrient Mixture F-12: Stored in a sealed container in the fridge. Because of its sterility, this is handled in a Class.II cabinet. Wear lab coat and gloves. Dulbeccos Modified Eagles medium: : Stored in a sealed container in the fridge. Because of its sterility, this is handled in a Class.II cabinet. Wear lab coat and gloves. Fungizone: Stored in a sealed container in the freezer. Wear lab coat and gloves. Adenine: Stored in a sealed container in the freezer. Wear lab coat and gloves. Glutamine: Stored in a sealed container in the freezer. Wear lab coat and gloves. Penicilin: Stored in a sealed container in the freezer. Wear lab coat and gloves. Fetal Bovine Serum: Stored in a sealed container in the freezer. Wear lab coat and gloves. Tri iodo-L- Thyronine: Stored in a sealed container in the freezer. Only handle in a Class II cabinet. Wear lab coat and gloves.

Emergency Procedures: a) Spillage
For the amounts that we are working with, wear lab coat, face mask, safety specs and gloves; ventilate area, wipe up spilt material and dispose of in yellow biohazard bins. Wash up area. .

b) Fire Unless stated otherwise, use extinguisher appropriate to surroundings.
Hydrochloric acid: Carbon Dioxide, dry chemical powder or appropriate foam.

Information Sources:
Sigma MSDS

Could a Less Hazardous Substance be used? If so, why not use it? No

First Aid Treatment:
Inhalation – remove to fresh air Exposure to eye/skin – wash with H2O for 15 minutes, if irritation develops seek medical advice Ingestion – seek medical advice, stating exact chemicals that you have been exposed to

Waste Disposal Procedures:
Inactivate solution (denaturing any active components – proteins etc) by placing in bleach pot (water material with bleach tablet in bucket – at least 2500 ppm available chlorine). Flush waste down sink after dilution with water once deactivated – this is to avoid corrosion of steel sink with chlorine. Check inactivation with storage (in incubator) and examination representative samples

Name of Assessor:

I have read this document and approve the above risk assessment

Status of Assessor: Signed:___________________________________

Signed:________________________________ Date:__________________________________ Date:_____________________________________