Nucleic acid purification service

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					Nucleic acids purification service

total RNA purification service genomic DNA purification service miRNA purification service

Total RNA purification service Total RNA purification service uses Trizol reagent combined spin-column method to purify RNA. The RNAs purified are measured by OD260nm, 260/280 ratio to determine the RNA quantity and purity. Also capillary electrophoresis using RNA 6000 nanochips run by Agilent Bioanalyzer 2100 are used to differentiate RNA integrity. Example for RNA QC report

RNA QC suggested OD260/280 ≥ 1.8
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28S/18S Ratio 1.2

RIN 7

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RIN : RNA Integrity Number

References for RNA QC: 1. RNA Quality: Defining the Good, the Bad, & the Ugly。 2. Ambion TechNote 11(1): Assessing RNA quality。 3. Ambion TachNote 11(2): Determinants of RNA Integrity and Purity。

Specimens types Cell line: Please collect ≥10 cells, and lyze them in the Trizol reagent (Invitrogen#15596-018). After cell lysis, the cell lysates are collected and stored in the -80 C freezer. More detail information; please see the sample RNA collection check list. Tissues: ≥ Tissues of 0.02 g is preferred. After surgery, tissues should be stored in LN2, and the whole process is suggested to be completed within 30 min. More detail information; please see the sample RNA collection check list. Regarding the effects of the tissue collection procedure to the RNA quality, please review the article RNA Quality: Defining the Good, the Bad, & the Ugly。 blood: Please collect blood ≥ 5 ml, stabilize the blood RNA in the Paxgene blood RNA tube (Pre Analytix), and store the blood mix in the -20 C freezer. More detail information, please see blood RNA collection check list.
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Total DNA purification service Total DNA purification service uses spin-column method to purify DNA. The DNAs purified are measured by OD260nm, 260/280 ratio to determine the DNA quantity and purity. Also single-stranded DNA length profile of each sample is checked by RNA 6000 nanochips run on Agilent Bioanalyzer 2100. Example for DNA QC report

Specimens types Cell line: Please culture ≥10 cells, scratch the cells, and stored in the -80 C freezer. More detail information, please see the sample DNA collection check list. tissues: ≥ Tissues of 0.02 g is preferred. After surgery, tissues should be stored in LN 2. More detail information, please see the sample DNA collection check list. blood: Please collect blood ≥ 1 ml in the blood tube with anticoagulation (heparin tubes are not suggested). After centrifugation to phase separation, buffy coat is aspirated and stored in the -80 C freezer. More detail information, please see the sample DNA collection check list.
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miRNA purification service Total RNA purification service of Welgene use Trizol reagent combined spin column method to purify miRNA. The miRNAs (<200nt.) and total RNA (>200nt.) purified are measured by OD260nm, 260/280 ratio to determine the RNA quantity and purity. Both miRNA and total RNA are examined by RNA 6000 nanochips run on Agilent Bioanalyzer 2100 to differentiate RNA integrity. PDF & other downloaded files\miRNA_Bioanalyzer.pdfThe relative publication by Agilent Technologies has showed the ability of Bioanalyzer 2100 to analyze small RNAs. Example for miRNA QC report

Specimens types Cell line: Please collect ≥10 cells, and lyze them in the Trizol reagent (Invitrogen#15596-018). After cell lysis, the cell lysates are collected and stored in the -80 C freezer. More detail information; please see the sample RNA collection check list. Tissues: ≥ Tissues of 0.02 g is preferred. After surgery, tissues should be stored in LN2, and the whole process is suggested to be completed within 30 min. More detail information; please see the sample RNA collection check list. Regarding the effects of the tissue collection procedure to the RNA quality, please review the article RNA Quality: Defining the Good, the Bad, & the Ugly。 blood: Please collect blood ≥ 5 ml, stabilize the blood RNA in the Paxgene blood RNA tube (Pre Analytix), and store the blood mix in the -20 C freezer. More detail information, please see blood RNA collection check list.
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posted:11/13/2009
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