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Laboratory Safety SOP Development Guidance Template - MCG by Levone

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									LABORATORY
STANDARD OPERATING PROCEDURE DEVELOPMENT GUIDANCE TEMPLATE Please edit and complete as necessary to address Biosafety Risks within your laboratory

Date: Principal Investigator: Department: Room(s) + BSL of each room: Phone: ______________________________________________________________________________ 1. Access to the laboratory is limited to staff, or other persons with permission of the Principal Investigator, when work with BSL-2 pathogens is being conducted. Access is limited according to attached procedures. 2. All laboratory personnel must be screened by Employee Health before working with potentially infectious materials, including fresh, unfixed, human or non-human primate specimens, uncharacterized cell lines, etc. Any available vaccinations which would reduce the risks associated with exposure to any of the agents in the protocol must be offered to all personnel or a signed waiver must be obtained by the PI. 3. Persons who are increased risk of infection, or for whom infection may have serious consequences, must not be allowed to enter laboratory when work with infectious agents are in progress without permission of the Principal Investigator. 4. All persons entering the laboratory must be advised of potential hazards. Laboratory workers must be trained and made aware of the hazards and appropriate safety precautions before working with any of the biological agents. 5. Spills and accidents that result in overt exposures to infectious materials must be immediately reported to the Principal Investigator and appropriate medical evaluation must be provided. 6. Laboratory staff must not eat, drink, smoke, handle contact lenses, chew gum, apply cosmetics in laboratory. 7. Food or drink for human consumption or utensils or cups must stored outside laboratory work area in refrigerators designated for that purpose only. 8. Laboratory staff and all other persons working with infectious substances must wear gloves. Contaminated gloves must be changed IMMEDIATELY. Under NO CIRCUMSTANCES will gloves be reused.
Application Date: _______________
101206

Principal Investigator: ___________________________________________ 1

9. Laboratory staff must wash hands after handling infectious materials, after removing gloves, and before leaving the laboratory. 10. Face protection (goggles, mask, face shield, or other splatter guard) must be used for all procedures when such procedures could produce splashes or sprays of infectious or other hazardous materials or when microorganisms are manipulated outside the biological safety cabinet. See attached procedures for more detailed PPE requirements. 11. Protective clothing must be removed and left in laboratory before going to non-laboratory areas (cafeteria, library, administrative areas). 12. Protective clothing must be either disposed of in laboratory or laundered by institution. (NEVER taken home!) 13. Only mechanical pipetting devices must be used in the laboratory. 14. All sharps must be placed in red (approved) sharps containers. 15. Sharps containers must be turned in when ¾ full. 16. All procedures must be performed to minimize creation of splashes or aerosols according to attached procedures. 17. A Biosafety Cabinet must be used for all procedures with potential for creating infectious aerosols or splashes, or whenever handling high concentrations of infectious materials. 18. Biological Safety Cabinet must have a current (annual) certification. 19. Biological Safety Cabinet must not be used until it is recertified after relocation, any movement, or dislocation. 20. Centrifuges used for high concentrations or large volumes of infectious agents must have sealed rotor heads or centrifuge safety cups. Safety cups must be opened only in a Biosafety cabinet. 21. All infectious animal carcasses must be disposed of by placing in a plastic bag and placing in infected animal carcass freezer or according to attached procedures. 22. All non-liquid contaminated cultures, stocks, plasticware and other biologically contaminated waste must be disinfected and placed in biomedical waste containers for disposal according to attached procedures. 23. All infectious liquids must be decontaminated or disinfected prior to being poured into drain according to attached procedures. 24. All work surfaces must be decontaminated at the completion of work, at the end of the day, or after any spill or splash of viable material according to attached procedures. 25. Laboratory equipment, surfaces and other contaminated materials must be decontaminated with an effective disinfectant on a routine basis, after work with infectious materials is finished, and especially after overt spills, splashes, or other contamination according to attached procedures. Equipment must be decontaminated before removal from the laboratory (for repair maintenance or other purposes). Decontamination protocol is attached. 26. Laboratory doors must be locked when the laboratory is not occupied.
Application Date: _______________
101206

Principal Investigator: ___________________________________________ 2

27. Spaces between benches, cabinets, and equipment must be kept accessible for cleaning. 28. Eyewash must be flushed at least monthly and kept clear of obstructions. 29. All personnel must know the location of the nearest safety shower and must ensure that area under the shower is kept free of obstructions. 30. Any personnel with overt exposure will be taken to Employee Health and/or the ER for immediate (<1 hour) post-exposure medical intervention and monitoring. 31. Any overt exposures of personnel to biological agents will be reported to the PI and the BSO as soon as possible.

Application Date: _______________
101206

Principal Investigator: ___________________________________________ 3

SOP ADDENUM- DETAILED LABORATORY-SPECIFIC PROTOCOLS
1. Storage and access control of cells or cultures include the following. Consider storage in all locations, including freezers, cryotanks, cold rooms. Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No No No No No Agents are stored inside well-labeled tubes/vials. Agents are stored inside well-labeled storage boxes. Biohazard labels are clearly affixed on any storage device clearly identifying the hazards inside Storage refrigerators/freezers/cryotanks are in rooms whose access is limited to authorized personnel. Refrigerators are kept locked and accessible only to authorized personnel Freezers are kept locked and accessible only to authorized personnel Cryotanks are kept locked and accessed only by authorized personnel Lockboxes are utilized and accessible only to authorized personnel Policies and procedures are in place whereby only persons who have been advised of the potential hazard and meet any specific entry requirements (e.g., immunization) are authorized to enter the laboratory or have access to agents. No Others (please specify)

2.

Procedures employed which may result in the generation of aerosols, splashes and/or sprays of biological material and safety precautions that should be followed by personnel performing these procedures are as follows: Centrifugation Sonication Vortexing Homogenization Opening Vacuum vials Syringe filtration Placing biological materials under pressure (through small bore hoses or needles) Animal cage changing Intranasal inoculation Necropsy of infected animals Fluorescence activated cell sorting Fluorescence activated cell analysis Other (please specify):

Safety precautions (e.g. use of Biosafety Cabinets, shields, respirators, containment devices):

3. Transport procedures for transport of agent(s)* between locations (i.e. through hallways and other non-laboratory areas). Note: For BSL2 agents, consider transporting agents inside a sealed, leakproof primary container inside a well-labeled sealed, leakproof, durable secondary container. *If transporting animals, please describe procedure. *If transporting any biological agent off-campus, please refer to Biosafety Schedule L for transport guidelines.

Application Date: _______________
101206

Principal Investigator: ___________________________________________ 4

4. Disinfection/decontamination and disposal of viable agents or recombinant DNA or items which have come in contact with these agents. See: http://www.mcg.edu/research/ibc/disinfectants for guidance in selecting appropriate disinfectants for the agents in this protocol. LIQUID WASTES (check all that apply): Disinfection/Decontamination Method Treatment time (eg. 30 min) N/A Final Disposal (after disinfection indicated in left column)) Check all that apply. If “other”, please specify below In sharps container In sharps container In sharps container As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste As chemical waste Other Other Other Other Other Other Other Other Other Other

None necessary Autoclaving Chemical Methods: Bleach (freshly diluted to final 10% (v/v)) Other chlorine products (Clidox®) Iodine/iodophors (eg. Wescodyne®) Alcohols (e.g. final 70% (v/v) EtOH or IPA) Phenolic agents (e.g. Biozide®, Vesphene®) Quaternary Ammonium Agents (e.g. Roccal®, Coverage Plus®, Cavicide®, Lysol®) Aldehydes (e.g. 2-4% glutaraldehyde, 4% formaldehyde, Cidex®) Peroxygens (e.g. Virkon®) Other, please specify:

Into Sewer Into Sewer

SOLID WASTES (Plasticware, glassware, tubes, tissues) (check all that apply): Disinfection/Decontamination Method Treatment time (eg. 30 min) N/A Final Disposal (after disinfection indicated in left column) Check all that apply. If “other”, please specify below All biological waste must be disposed as Biohazardous Waste In Biohaz. box In Biohaz. box In Biohaz. box In Biohaz. box In Biohaz. box In biohaz. sharps container In biohaz. sharps container In biohaz. sharps container In biohaz. sharps container In biohaz. sharps container Other Other Other Other Other

None required Autoclaving Chemical Methods Bleach (freshly diluted to final 10% (v/v)) Aldehydes (e.g. fixation with 2-4% glutaraldehyde, 4% formaldehyde) Peroxygens (e.g. Virkon®) Other, please specify:

ANIMAL TISSUES/CARCASS WASTE (check all that apply): Disinfection/Decontamination Method Treatment time (eg. 30 min) N/A Final Disposal (after disinfection indicated in left column) Check all that apply. If “other”, please specify below In LAS animal disposal freezer In LAS animal disposal freezer In Biohaz. waste box In Biohaz. waste box Other Other

Autoclaving prior to removal from laboratory None, transported to LAS as per protocol below Other, please specify:

Application Date: _______________
101206

Principal Investigator: ___________________________________________ 5

5.

Work surfaces, instruments, equipment will be decontaminated using the following method(s). Consider both benchtop surfaces and stainless steel surfaces (such as those in biosafety cabinets), as well as in equipment (such as centrifuges) and instruments (check all that apply): Check all that apply Stainless Equipment Surfaces

Disinfection/Decontamination Method

Contact time (e.g. 30’)

Frequency (e.g. daily and after spills)

Benchtops

Instruments

Autoclaving Chemical Methods: Bleach (freshly diluted to final 10% (v/v)) Bleach (freshly diluted to final 10% (v/v)), + rinse with 70% Alcohol Other chlorine products (Clidox®) Iodine/iodophors (eg. Wescodyne®) Alcohols (e.g. final 70% (v/v) EtOH or IPA) Phenolic agents (e.g. Biozide®, Vesphene®) Quaternary Ammonium Agents (e.g. Roccal®, Coverage Plus®, Cavicide®, Lysol®) Aldehydes (e.g. 2-4% glutaraldehyde, 4% formaldehyde, Cidex®) Peroxygens (e.g. Virkon®) Other, please specify:

6. Spills involving biological material will be handled in your laboratory using the following procedures (check all that apply): (a) Describe which measures will be employed to handle spills in your laboratory: Large spills or high-hazard spills will be reported to PI and BSO immediately for assistance Spills in public (non-laboratory) areas will be reported to PI and BSO immediately Spills involving high hazard agent(s) (e.g. Select Agents) will be reported to the PI and BSO immediately Any injured individuals will be removed from areas and medical attention and follow-up will be provided All personnel in area will be advised of hazard and told to avoid the area Spill will be covered with copious absorbent material (like paper towels) The spill will be decontaminated with an appropriate disinfectant for the agent(s) spilled for an appropriate contact period (see below) After decontamination, absorbent material will be disposed as biohazardous waste After decontamination, the surface will be cleaned to remove residual disinfectant Spills in equipment (e.g. Biosafety cabinets or centrifuges) will be disinfected according to written procedures on the MCG Emergency Response Flip charts which have been posted in my laboratory

Application Date: _______________
101206

Principal Investigator: ___________________________________________ 6

(b) Spills will be disinfected using the following disinfectant and contact time: Disinfection/Decontamination Method Autoclaving Chemical Methods: Bleach (freshly diluted to final 10% (v/v)) Bleach (freshly diluted to final 10% (v/v)), + rinse with 70% Alcohol Other chlorine products (Clidox®) Iodine/iodophors (eg. Wescodyne®) Alcohols (e.g. final 70% (v/v) EtOH or IPA) Phenolic agents (e.g. Biozide®, Vesphene®) Quaternary Ammonium Agents (e.g. Roccal®, Coverage Plus®, Cavicide®, Lysol®) Aldehydes (e.g. 2-4% glutaraldehyde, 4% formaldehyde, Cidex®) Peroxygens (e.g. Virkon®) 7. Protective equipment which will be utilized to minimize exposure of personnel to agents while in the laboratory: Contact time (e.g. 30’)

(check all that apply): Gloves (nitrile or latex) Double Gloves (nitrile or latex) Lab coat Tyvek coveralls Rear-closing gowns Other (please specify)

Cuffed wrists on gowns Sleeve protectors Scrubs Shoe covers/booties Biosafety Cabinet

Safety glasses Goggles Face shield Plexiglass shield Safety caps/Sealed rotors on Centrifuge

Surgical mask N95 Respirator PAPR Respirator Aprons Rubber boots

8. Protective equipment which will be utilized to minimize exposure of personnel to agents while working with animals (if applicable):
Agents will be delivered into animals using which BSL containment practices and standards: BSL-1 BSL-2 BSL-3

Please indicate Personal Protective Equipment (PPE) and other safety equipment to be utilized while delivering agents into animals (check all that apply): Gloves (nitrile or latex) Cuffed wrists on gowns Safety glasses Surgical mask Double Gloves (nitrile or latex) Sleeve protectors Goggles N95 Respirator
Lab coat Tyvek coveralls Rear-closing gowns Scrubs Shoe covers/booties Biosafety Cabinet Face shield Plexiglass shield PAPR Respirator Aprons Rubber boots

Other (please specify): ______________________________________________________________________________ After delivery of agents into animals: After delivery of agents, animals will be housed in (check all that apply): Separate cages from other animals Microisolator cages Well-labeled cages indicating possible hazards to animal staff Conventional/ABSL-1 facilities ABSL-2 facilities Cages with filter-top lids ABSL-3 facilities

Live animals will not be returned to LAS facilities after delivery

Other (please specify): _________________________________

How long after delivery of agents will animals be analyzed and/or sacrificed (indicate time range)? _________________________________

Application Date: _______________
101206

Principal Investigator: ___________________________________________ 7


								
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