Laboratory SOP INDEX by langkunxg

VIEWS: 0 PAGES: 6

									                                              Aerobiology Biomarkers Lab
                                    Laboratory Standard Operating Procedures (SOPs)



SOP Number       Title                                                  Status        Approval Date Last Revision Date
                 Determination of 3-OHFA in House Dust by GC/MS         approved
L-001                                                                                      5/10/2006
L-002            Determination of Endotoxin using the LAL Assay         approved           3/31/2006
L-003            Determination of Endotoxin using the rFC Assay         approved           5/10/2006
L-004            N-acety Muramic Acid by GC/MS                          in process
L-005            Glucan Assay                                           not started
                 Determination of Ergosterol in House Dust by GC/MS     approved
L-006                                                                                     12/19/2006           4/9/2007
L-007            Preparation of TAP Buffer                              approved           3/28/2006           5/9/2006
L-008            Cleaning/ Baking Glassware & Utensils                  approved            6/7/2007          7/12/2007
L-009            Data Analysis using the Klare Method                   in process
L-010            Sensitivity Test for rFC Plate Reader                  in process
L-011            Seiving of Loose Dust Samples & Storage                not started
L-012            Chemical Ordering, Receiving, & Handling               approved           6/28/2008
L-013            Sterilizing Caps                                       approved           4/24/2006
L-014            Silica Desiccant Regeneration                          approved           4/24/2006           6/7/2007
L-015            Preparation of PBS (pH 7.4)                            approved            6/8/2007
L-016            Spike Recovery for Ergosterol in Dust Samples using    approved
                 GC/MS                                                                      5/1/2007           8/8/2007
L-017            Spike Recovery for 3-OHFA in Dust Samples using        approved
                 GC/MS                                                                      5/1/2007
L-018            Preparation of 3-OHFA Standard Curve                   approved           4/20/2007          6/22/2007
L-019            Preparation of Validation Controls for 3-OHFA          approved           2/19/2008
L-020            Determination of 3-OHFA in House Dust Samples          approved
                 using Ion Trap GC/MS                                                      7/23/2007
L-021            Preparation of Pellets & Extracts for Analysis         approved           7/25/2007
L-022            Preparation of an Ergosterol Standard Curve            approved
                                                                                           4/25/2007           8/8/2007
L-023            Preparation of Validation Controls for Ergosterol      approved
                 Assays                                                                    4/25/2007
L-024            Determination of Ergosterol in Dust Samples using      approved
                 GC/MS                                                                      5/1/2007           8/8/2007
L-025            Determination of Endotoxin in House Dust Samples       in process
                 using the rFC PyroGene Kit by Cambrex
L-026
                 LIMS for Access and Management of Laboratory Data      approved           9/25/2007
L-027            Data Handling for the GC/MS                            approved           7/27/2007          9/10/2007
L-028            Spike Recovery with 13C labelled Yeast for Measuring
                 ergosterol in Dust Samples                             approved            8/9/2007
L-029            Determination of ergosterol in House Dust Samples
                 with 13C Yeast Internal Standard                       approved            8/9/2007
L-030
                 Preparation of an Ergosterol Standard Curve (1.5625-
                 100ng range) with 13C Yeast as Internal Standard       approved            8/9/2007
L-031            Preparation of 13C Labelled Yeast                      not started
L-032
                 Transferring Data from Varian Computer to ALICE        approved           1/30/2008
L-033            Determination of Endotoxin using the rFC Assay         approved            8/1/2008

        C:\Docstoc\Working\pdf\81890293-3333-43c7-a6a6-763045a57806.xls
        5/27/2013 10:59 PM
                                               Aerobiology Biomarkers Lab
                                     Laboratory Standard Operating Procedures (SOPs)



L-034            Preparation of 70% Ethanol
L-035            MDCK cell culture propagation & maintenance
L-036            Influenza focus reduction assay, incl 96-well plate
                 seeding
L-037
L-038
L-039
L-040
L-041
L-042




        C:\Docstoc\Working\pdf\81890293-3333-43c7-a6a6-763045a57806.xls
        5/27/2013 10:59 PM
                                          Aerobiology Biomarkers Lab
                                 Laboratory Standard Operating Procedures (SOPs)



Use Status

superseded by L-020
current
replaced by L-033
under revision


superseded by L-029
current
current
under revision
under revision

current
current
current
current

obsolete

current
current
current

current
current

superseded by L-030

current

superseded by L-029


replaced by L-033

current
current

current

current


current


current
current

      C:\Docstoc\Working\pdf\81890293-3333-43c7-a6a6-763045a57806.xls
      5/27/2013 10:59 PM
                                    Aerobiology Biomarkers Lab
                           Laboratory Standard Operating Procedures (SOPs)




C:\Docstoc\Working\pdf\81890293-3333-43c7-a6a6-763045a57806.xls
5/27/2013 10:59 PM
                                 Aerobiology Biomarkers Lab
                       Equipment Standard Operating Procedures (SOPs)




SOP Number   Title                                               Status        Approval Date Last Revision Date
E-001        Calibration & Use of Mettler-Toledo AG285 Balance
                                                                 approved            6/7/2007
E-002        General Use and Maintenance of the GC/MS            in process
E-003        General Use of the Vortex Evaporator                approved           4/24/2006          5/9/2006
E-004        Calibration & Use of the Mettler-Toledo AB104-5
             Balance                                             approved            6/7/2007
E-005        General Use of the Fisher Scientific Oven           approved            6/8/2007
E-006        General Use & Calibration of the Beckman Φ340 pH
             Meter                                               approved           3/31/2006         7/12/2007
E-007        General Use of the Labconco Dishwasher              approved           6/28/2007
E-008        General Use of the Sterilmatic Steam Sterilizer
             (Autoclave)                                         approved           6/25/2007
E-009        Use of the BIO-TEK Instruments ELx808 Incubating
             Plate Reader                                        in process
E-010        General Use of the Baker Class II Type A2 Biohood   not started
E-011        General Use of the Heating Block                    not started
E-012        General Use of the Heraeus Centrifuge               approved           6/28/2007
E-013        Use of the BIO-TEK Instruments FLx800 Fluorescent
             Plate Reader                                        in process
E-014        General Use of the Nitrogen Evaporator "MultiVap"   not started
E-015        General Use & Maintenance of the Lyophilizer        approved           4/20/2007
E-016        Computer Maintenance Procedures                     approved           6/25/2007
E-017        Freezer Inventory Backup on Computer                approved           6/28/2007
E-018        Plater Reader Decontamination                       approved           6/28/2007
E-019        How to change a Tank (Gas Cylinder)                 approved            2/4/2008
E-020
E-021
E-022
E-023
E-024
E-025
E-026
E-027
L-028




                                                    5/27/2013
      Aerobiology Biomarkers Lab
Equipment Standard Operating Procedures (SOPs)




                   5/27/2013

								
To top