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					                                                               Photometric water analysis

The NANOCOLOR ® analytical system with quality control
NANOCOLOR ® is a complete system for photometric water                special chemicals for special applications like sample
analysis. It comprises                                                preparation and elimination of interferences
   valuable photometers                                               numerous analytical accessories: piston pipettes, mem-
   user-friendly tube tests                                           brane filters, glassware etc.

   standard tests for highest precision                               the NANOCONTROL system for analytical quality con-
                                                                      trol – consisting of standard solutions, and
   heating blocks for sample decomposition (COD, AOX,                 NANOCONTROL NANOCHECK
   total N etc.)
                                                                      service information – seminars, certificates, EC safety
   time-saving microwave decomposition                                data sheets, service routines, etc.




                                          NANOCOLOR ® · the system for photometric water analysis
                                             Advantages of the NANOCOLOR ® analytical system                            64 – 65
                                             NANOCOLOR ® photometers and accessories                                    66 – 69
                                             NANOCOLOR ®      heating blocks                                            70 – 71
                                             NANOCOLOR ® tube tests                                                     72 – 73
                                             NANOCOLOR ® standard tests                                                         74
                                             NANOCOLOR ® extraction methods                                                  75
                                             Description of individual parameters and tests                             76 – 90
                                             NANOCOLOR ® digestion methods                                              91 – 96
                                             Digestion methods for total-Nitrogen, total-Phosphorus
                                             and heavy metals                                                           93 – 96
                                             Special NANOCOLOR ® procedures
                                             Sum parameters AOX, BOD5, COD Hg-free, Hydrocarbons, TTC                  98 – 102
                                             NANOCOLOR ® accessories                                                  104 – 106

                                          Analytical quality control
                                             NANOCONTROL standards and NANOCONTROL NANOCHECK                          107 – 109
                                             Service information                                                               110




www.mn-net.com                                                                                                            MN         63
                The system for photometric water analysis

                               Advantages of the NANOCOLOR ® analytical system




                               Photometer 500 D                                Photometer 400 D
                                           bar-code reading photometers




                                                                                            with
                                                                                                   bar
                                                                                                         cod
                                                                                                            e
                                                                                                               tube tests
                                         the system for                                                user-friendly with
     standard tests                 photometric water analysis                                       predosed reagents
       for highest precision




                                NanOx
                                solid reagents
                                for oxidative decomposition
                                                                              numerous analytical accessories



                                                     decomposition systems



                                                        with microwave
                                                                         or heating block




64    MN                                                                                                   www.mn-net.com
                                   The system for photometric water analysis

                         Advantages of the NANOCOLOR ® analytical system

   Analytical quality control                 NANOFIX reagent capsules
                                              for convenient reagent dosage
                                              (Patent EP 92 101 316.5)
                                                 freeze-dried reagents in plastic
                                                 capsules
                                                 ➜ no spilling of powder
                                                 ➜ immediate dissolution of
                                                     reagents
                                                 ➜ minimal exposure to
                                                     chemicals
                                                 predosed content of the
                                                 capsules
                                                 ➜ exact reagent dosage
                                                 ➜ more precise results

The NANOCONTROL system
   standard solutions and multistandards
   standard addition NANOCONTROL 100 +
   NANOCONTROL interlaboratory trial
   NANOCONTROL NANOCHECK




                 We will be glad to help you find the optimum combination for your needs!




www.mn-net.com                                                                              MN   65
                    The system for photometric water analysis

     NANOCOLOR ® photometers

     NANOCOLOR ® 500 D                                                  NANOCOLOR ® 400 D
            The digital photometer for universal use in                        The economical photometer for routine
            water and waste water analysis                                     analyses




                                                      NEW!




        Illuminated graphic display                                        2-line LC display
        USB interface for data transfer
        Additional measuring programme
        ✓   Transmission
        Data memory for up to 500 values                                   Data memory for up to 999 values


     Versatile applicability for every demand

                                                    NANOCOLOR ® 500 D / 400 D
     Unique versatile                                                   Comfortable updating and data processing
     The digital photometer  NANOCOLOR ®      500 D is the conse-          State of the art interfaces
     quent development of our very successful photometer                   Fast photometer update via Internet/PC
     NANOCOLOR ® 400 D. Adapted to our customers’ require-                 GLP compatible measurement documentation on PC or
     ments, today and tomorrow, it is suitable for universal use in        printer
     water and wastewater analysis. This includes municipal and            User-friendly data export software
     industrial wastewater, drinking water, surface water, ground
     water as well as cooling and boiler water.
                                                                           ✓ data transfer in MS Excel / MS Access
                                                                           ✓ recording of calibration curves for programming of
     NANOCOLOR ® Bar Code Technology (NBT)                                     user defined methods
     Fully automatic, instant cuvette detection by the built-in laser      Direct print-out on the NANOCOLOR ® thermal printer
     scanner allows simple and fast routine analysis. Selection of         (Cat. No. 919 16)
     the test method and suitable wavelength, the actual measure-       In the field and in the lab
     ment and storage of the measurement result are carried out
     automatically without having to use any other key.                 The standard built-in, robust high-performance battery with
                                                                        charge regulator allows continuous operation at 220 V
     Simple and clear user guidance                                     (110 V) and up to 3000 on-site mains-independent measure-
     The newly designed user guidance allows safe operation and         ments. The battery charge status is shown in the graphic dis-
     facilitates your daily work. In the configuration menu, you can    play. In the power saving mode, the photometer switches off
     select up to 10 languages (D, GB, F, E, I, NL, H, PL, P, CZ).      itself after 10, 20… 120 min.
     Convenient memory management                                       Universal cuvette holder
     Automatic storage can be switched on and off in the configu-       Round tubes (14 mm ID) and rectangular cuvettes (10, 20
     ration menu. Moreover, individual measurement values can           and 50 mm) can be used without adaptor.
     be stored manually via the keypad at all times. Stored meas-       Versatile – Flexible - Safe
     urement values and data records can be accessed by meth-
     od, sample location, date and time.                                   Over 100 preprogrammed tests with over 200 analysis
                                                                           programmes of MACHEREY-NAGEL
                                                                           Programmable for 100 user defined applications, linear
                                                                           and non-linear methods
                                                                           Automatic functional test and auto-calibration
                                                                           Professional photometric basic functions (extinction,
                                                                           transmission, measurement with factor, kinetics, two-
                                                                           point calibration)



66     MN                                                                                                            www.mn-net.com
                                     The system for photometric water analysis

NANOCOLOR ® Photometer
Everything at a glance
The modern, illuminated graphic display of the
NANOCOLOR ® 500 D with user guidance shows all impor-
tant data at a glance.
    Measurement result, displayed directly in the required
    measuring unit
    Date / time
    Battery charge status
    Sample number (4 characters)
    Sample location (alphanumeric, 12 characters)
    Dilution
    Memory on / off

Technical data
                          NANOCOLOR ® 500 D                                    NANOCOLOR ® 400 D
Type:                     Single beam filter photometer with microproces-       Single beam filter photometer with microproces-
                          sor control, self-test and auto-calibration, wave-   sor control, self-test and auto-calibration, wave-
                          length range 340 – 860 nm                            length range 340 – 860 nm
Optics:                   automatic filter wheel with 10 interference filters    automatic filter wheel with 10 interference filters
Wavelength:               345 / 365 / 436 / 470 / 520 / 540 /                  345 / 365 / 436 / 470 / 520 / 540 /
                          585 / 620 / 690 / 800 nm                             585 / 620 / 690 / 800 nm
                          plus 2 compartments for additional filters            plus 2 compartments for additional filters
Wavelength                ± 2 nm, bandwidth at half transmission 10 – 12 nm    ± 2 nm, bandwidth at half transmission 10 – 12 nm
accuracy:
Light source:             Tungsten lamp                                        Tungsten lamp
Detector:                 silicon photodiode                                   silicon photodiode
Blank value:              automatic and prestored                              automatic and prestored
Measuring modes:          over 100 preprogrammed tests,                        over 100 preprogrammed tests,
                          100 freely programmable methods                      100 freely programmable methods
                          extinction, transmission, factor, kinetics,          extinction, factor, kinetics,
                          two-point calibration                                two-point calibration
Measuring range:          about 3 E without sign                               about 3 E without sign
Photometric               ± 1%                                                 ± 1%
accuracy:
Stability:                < 0.002 E/h                                          < 0.002 E/h
Cuvette holder:           round tubes 14 mm ID                                 round tubes 14 mm ID
                          rectangular cuvettes 10, 20, 50 mm                   rectangular cuvettes 10, 20, 50 mm
Data memory:              500 measurement data records, GLP compatible         999 measurement data records, GLP compatible
Display:                  illuminated graphic display with user guidance,      2-line LC display, 8 languages
                          64 x 128 pixels, 10 languages
Operation:                bar code technology, display user guidance, foil-    bar code technology, display user guidance, foil-
                          covered push-buttons                                 covered push-buttons
Interfaces:               USB 1.1 and bidirectional serial RS 232              bidirectional serial RS 232 and parallel Centronics
Update:                   via Internet / PC                                    via Internet / PC
Operating range:          0 – 50 °C, up to 90 % relative humidity              0 – 50 °C, up to 90 % relative humidity
Power supply:             100 – 240 V~, 50/60 Hz / 6 V, 3.2 Ah via built-in    100 – 240 V~, 50/60 Hz / 6 V, 3.2 Ah via built-in
                          battery with charge regulator and mains power        battery with charge regulator and mains power
                          supply                                               supply
Dimensions:               227 x 282 x 105 mm                                   227 x 282 x 105 mm
Weight:                   2.4 kg                                               2.4 kg
Marking:                  CE                                                   CE
Warranty:                 2 years                                              2 years

        These appliances comply with the following EC directives:
        – 73/23/EEC of19.02.1973 – Low-Voltage Directive
        – 89/336/EEC of 03.05.1989 (including amendment directive 92/31/EEC) – EMV-Directive


www.mn-net.com                                                                                                               MN      67
                   The system for photometric water analysis

     NANOCOLOR ® photometers

     Up-to-date data processing

                                                   NANOCOLOR ® 500 D / 400 D
     Data processing options of the photometers NANOCOLOR ®
     500 D and 400 D offer:
        large data memory for up to 999 measurement values
        (500 for the 500 D)
        ✓ automatic storage of all values or user-defined stor-
            age of specific values via keypad
        ✓ memory storage optional (e. g. for repeat measure-
            ments)
        traceability of measured values
        ✓ built-in timer with date for data processing
        ✓ automatic assignment of date and time for every
            stored value
        ✓ optional assignment of sample location and sample           Data can be either written directly into an EXCEL worksheet
            number for every stored value                             or into an ACCESS data base. Because of the use of Win-
        ✓ clear identification of values above or below the           dows® standard software an easy further processing of the
            measuring range for stored values                         data is guaranteed.
        Free selection of individual values by method, sample lo-
        cation, sample number, date and time.
     Interfaces
        Both photometers are equipped with a serial RS 232 in-
        terface for data transfer to a PC or a direct print-out on    The program allows automatic construction of calibration
        the NANOCOLOR ® thermal printer (Cat. No. 919 16)             curves up to 4th degree and the calculation of all relevant sta-
        The photometer NANOCOLOR ® 500 D is additionally              tistical parameters such as coefficient of variation, standard
        equipped with a USB 1.1 interface for data transfer to a      deviation etc. and the preparation of calibration protocols.
        PC.
        ✓ measured values can be transferred to a computer
           automatically after every measurement or from the
           data memory
        ✓ data management is compatible with Windows® sys-
           tems
        ✓ data transfer to the PC can be performed with pro-
           grams from the Windows® accessories
        The photometer NANOCOLOR ® 400 D is additionally
        equipped with a Centronics interface.




     Data transfer to Windows® standard software:
     NANOCOLOR ® Photometer Data Export
     The fast availability and safe storage of analytical data with   Minimum system requirements: Pentium 2 (> 200 MHz)
     standard software is an important aspect in different kind of    processor, 40 MB free hard disk memory, 64 MB RAM, Win-
     laboratories.                                                    dows® 2000 or XP, a free serial interface or a USB-serial
                                                                      adaptor (with virtual serial interface driver), a USB interface
     The program NANOCOLOR ® Photometer Data Export al-
                                                                      (for the photometer NANOCOLOR ® 500 D) and a CD-ROM
     lows to transfer the data from the memory of the photometers
                                                                      drive for installation.
     NANOCOLOR ® 500 D, 400 D, LINUS, 350 D, 300 D and
     250 D to a PC or receive measurements online from the            To create MS-EXCEL or MS-ACCESS files you need MS Of-
      photometers (not with LINUS).                                   fice 97, 2000 or 2003. To connect the photometer with the PC
                                                                      you need a serial cable (zero modem cable, e.g. Cat. No. 919
                                                                      680). For the NANOCOLOR ® 500 D a USB cable can be
                                                                      used alternatively.

                                                                      Ordering information:
                                                                      NANOCOLOR ® Photometer Data Export Cat. No. 919 02



68     MN                                                                                                            www.mn-net.com
                                    The system for photometric water analysis

NANOCOLOR ® photometer and accessories
Ordering information

Description                                                                                                  Cat. No.
Photometers
Universal photometer NANOCOLOR ® 500 D incl. software CD, manual, protective covering, mains adaptor,        919 500
data cable, USB cable and calibration cuvette in stable transport bag
Universal photometer NANOCOLOR ® 400 D incl. manual, protective covering, mains adaptor, data cable and      919 70
calibration cuvette in stable transport bag

Accessories for photometers NANOCOLOR ® 500 D / 400 D / Linus / 350 D / 300 D / 250 D
Description                                                                                        Pack of   Cat. No.
Analytical quality control
NANOCONTROL NANOCHECK                                                                               1 set    925 701
Test solutions for the determination of photometric accuracy
Special filters for photometers NANOCOLOR ® 500 D / 400 D / 350 D / 300 D / 250 D
Special filters incl. ex factory installation (wavelengths on request)                                1       919 850.2
Manual for NANOCOLOR ® 500 D                                                                         1       919 501
Manual for NANOCOLOR ® 400 D                                                                         1       919 09
Cuvettes
Calibration cuvette for NANOCOLOR ® photometers                                                      1       916 908
Glass cuvettes, 5 mm optical path                                                                    2       919 32
Glass cuvettes, 10 mm optical path                                                                   2       919 33
Glass cuvettes, 20 mm optical path                                                                   2       919 34
Glass cuvette, 50 mm optical path                                                                    1       919 35
Semi-micro cuvette, 50 mm optical path                                                               1       919 50
Lids for glass cuvettes 10 mm                                                                        2       919 41
Lids for glass cuvettes 50 mm                                                                        2       919 40
Disposable plastic cuvettes, 10 mm optical path                                                     100      919 37
Lamps
Tungsten lamp for NANOCOLOR ® 500 D / 400 D / 350 D / 300 D / 250 D                                  1       919 787
Protective coverings
Protective covering for NANOCOLOR ® 500 D / 400 D                                                    1       919 18
Printer
NANOCOLOR ® thermal printer for NANOCOLOR ® 500 D / 400 D / 350 D / 300 D / 250 D and PF-11           1      919 16
Paper for NANOCOLOR ® thermal printer                                                              5 rolls   930 65
rolls of 58 mm width, core 12 mm, outer diameter 30 mm
Paper for NANOCOLOR ® thermal printer as above, outer diameter 45 mm                               5 rolls   930 71
Software
Data transfer software for photometers NANOCOLOR ® Linus / 500 D / 400 D / 350 D / 300 D / 250 D    1 CD     919 02
and luminometer BioFix ® Lumi-10 (German, English, French)
for data transfer required:
Zero modem cable, serial, 2x9 pin SUB-D socket, for NANOCOLOR ® 500 D / 400 D / 350 D /              1       919 680
300 D / 250 D and photometer PF-10 / PF-11
Adaptor, 9 pin SUB-D plug to 25 pin SUB-D socket                                                     1       919 681
Power supply
Mains adaptor for NANOCOLOR ® 500 D / 400 D / 350 D / 300 D / 250 D / PF-11 / PF-10;                 1       919 06
prim. 100 – 240 V ~; sec. 9 V = / 1500 mA
Rechargeable battery for photometers NANOCOLOR ® 500 D / 400 D / 350 D                               1       919 914
Accessories for photometers NANOCOLOR ® 25, 50 D, PT, PT-2, PT-3, 100 D, 200 D, 250 D, 300 D,
350 D, Linus and SM-1 are still available.




www.mn-net.com                                                                                                    MN     69
                    The system for photometric water analysis

     NANOCOLOR ® heating blocks

     NANOCOLOR ® VARIO 3 and VARIO compact                            Suitable for all NANOCOLOR ® digestion methods
     Programmable heating blocks for chemical-analytical digestions   Application                 Temperature           Time
        High-speed COD in 30 minutes
                                                                      COD in accordance to           148 °C           120 min
        Easy to operate via symbol keys                               ISO 15705
        Preprogrammed standard programmes for all routine             High-speed COD                 160 °C        30 min
        digestions                                                    TOC                            120 °C       120 min
        Programmable for user-defined digestion methods               Total nitrogen                 120 °C        30 min
                                                                      Total phosphorus               120 °C        30 min
        High temperature stability and short warm-up times            Organic acids                  100 °C        10 min
        External temperature control and calibration          with    Metals                         120 °C        30 min
        NANOCOLOR ® T-Set (Cat. No. 919 917)                          AOX                            120 °C        30 min
                                                                      Programmable, user-defined    30 – 160 °C 0:01 – 99:59 h
                                                                      programmes



     VARIO 3                                                          VARIO compact


                                                    NEW!                                                            NEW!




        Simultaneous digestion of 24 samples                             Simultaneous digestion of 12 samples
        2 independently usable heating units
        2 separate protective coverings

     Ordering information
      Description                                                                                          Pack of      Cat. No.
      Heating blocks
      Heating block NANOCOLOR ® VARIO 3                                                                         1       919 36
      incl.power cable, 2 separate protective coverings and manual
      Heating block NANOCOLOR ® VARIO compact                                                                   1       919 13
      incl. power cable, protective covering and manual
      Accessories for digestion in a heating block
      NANOCOLOR ® T-Set for electronic temperature control and calibration of heating blocks                    1       919 917
      NANOCOLOR ® VARIO 3 and VARIO compact
      Protective covering for heating blocks NANOCOLOR ® VARIO 3 and VARIO compact, transparent                 1       919 593
      Exchangeable safety cover for heating blocks NANOCOLOR ® VARIO 3 and VARIO compact                        1       919 598
      Reducing adaptors 22 →16 mm for NANOCOLOR ® heating blocks                                                2       919 916



70     MN                                                                                                           www.mn-net.com
                                     The system for photometric water analysis

NANOCOLOR ® heating blocks
Technical data

                  NANOCOLOR ® VARIO 3                                     NANOCOLOR ® VARIO compact
Type:             programmable heating block for chemical-analytical      programmable heating block for chemical-analytical
                  digestions with 24 holes for test tubes with 16 mm      digestions with 12 holes for test tubes with 16 mm
                  OD (outer diameter)                                     OD (outer diameter)
Display:          4 LED displays, 6 mm character height, display          2 LED displays, 6 mm character height, display
                  shows temperature and remaining heating time            shows temperature and remaining heating time
Operation:        foil-covered symbol keys                                foil-covered symbol keys
Temperatures:     5 preprogrammed temperatures                            5 preprogrammed temperatures
                  (70 / 100 / 120 / 148 / 160 °C); 4 free memory loca-    (70 / 100 / 120 / 148 / 160 °C); 4 free memory loca-
                  tions for individual temperature settings               tions for individual temperature settings
Temperature       30 - 160 °C (1 °C increments)                           30 - 160 °C (1 °C increments)
range:
Temperature       ± 1 °C (according to DIN, EN, ISO and EPA methods) ± 1 °C (according to DIN, EN, ISO and EPA methods)
stability:
Warm-up time:     from 20 °C to 160 °C within 10 minutes                  from 20 °C to 160 °C within 10 minutes
Heating times:    4 preprogrammed heating times                           4 preprogrammed heating times
                  (0:30 h, 1:00 h, 2:00 h, cont.)                         (0:30 h, 1:00 h, 2:00 h, cont.)
                  5 free memory locations for individual time settings    5 free memory locations for individual time settings
Time range:       0:01 – 99:59 h (0:01 h increments)                      0:01 – 99:59 h (0:01 h increments)
Safety devices:   2 integrated protective coverings as protection         integrated protective coverings as protection against
                  against excess temperature                              excess temperature
                  exchangeable safety covers as protection against        exchangeable safety covers as protection against
                  contact                                                 contact
Equipment         bidirectional serial RS 232 allows connection with      bidirectional serial RS 232 allows connection with
inspection        NANOCOLOR ® T-Set                                       NANOCOLOR ® T-Set
monitoring:
Interface:        bidirectional serial RS 232                         bidirectional serial RS 232
Update:           via RS 232 and PC                                   via RS 232 and PC
Power supply:     110 – 230 V ~, 50/60 Hz                             110 – 230 V ~, 50/60 Hz
Power             250 / 500 VA                                        125 / 250 VA
consumption:
Dimensions:       255 x 250 x 140 mm                                  155 x 250 x 140 mm
Weight:           ca. 3.85 kg                                         ca. 1.8 kg
Marking:          CE                                                  CE
Warranty:         2 years                                             2 years
       Declaration of conformity:
       These instruments conform to the following directives
       -89/336/EEC of 03.05.1989 (including Amendment Directive 92/31/EEC) – EMV Directive

NANOCOLOR ® T-Set
Calibrated temperature sensor with electronic for external temperature control, fully automatic calibration and prepara-
tion of a test certificate for instrument control and monitoring
Technical data
Detector:                PT 1000 (95 x 4 mm)                         Interfaces:            bidirectional serial RS 232
Accuracy:                ± 0.1 °C                                    Operating range:       0 – 50 °C, up to 90 % relative
Display:                 via LED-displays of the heating                                    humidity
                         block and the NANOCOLOR ®                   Power supply:          via RS 232
                         T-Set software                              Power consumption:     max. 20 mW
Operation:               via foil-covered symbol keys                Dimensions:            112 cm (length)
                         of the heating block and the
                                                                     Weight:                ca. 60 g
                         NANOCOLOR ® T-Set software
                                                                     Marking:               CE
Temperature range:       0 – 200 °C
                                                                     Certificate:            calibrated against adjusted
Temperature stability:   ± 0.2 °C
                                                                                            thermometers
Long term stability:     ± 0.1 °C
                                                                     Warranty:              2 years

www.mn-net.com                                                                                                               MN   71
                     The system for photometric water analysis

     NANOCOLOR ® tube tests

     The perfect tool for routine analysis in sewage plants and industry
          Precisely predosed reagents in 16 mm tubes
          Accurate reagent dosage with NANOFIX capsules
          Measurement directly in the test tube
          Minimal exposure to chemicals
          Reduced reagent consumption
          Less disposal problems
          Bar-code cuvette identification
          No preparation of blank values necessary
          Timesaving and easy-to-follow procedures
          Fast and reliable results


     NANOCOLOR ® tube tests · product information for photometers 500 D, Linus, 400 D, 350 D, 300 D and 250 D
     Test                                   Measuring ranges for NANOCOLOR ® photometers         Wavelength     No. of     Cat. No.
                                                                                                                tests
     Alcohol, see Ethanol and Methanol
     Aluminium 07                    0.02 – 0.70 mg/l Al3+                                         540 nm        19       985 098
     Ammonium 3                      0.04 – 2.30 mg/l NH4-N           0.05 – 3.00 mg/l NH4+        690 nm        20       985 003
     Ammonium 10                     0.2 – 8.0 mg/l NH4-N             0.2 – 10.0 mg/l NH4+         690 nm        20       985 004
     Ammonium 50                     1 – 40 mg/l NH4-N                1 – 50 mg/l NH4+             690 nm        20       985 005
     Ammonium 200                    30 – 160 mg/l NH4-N              40 – 200 mg/l NH4+           585 nm        20       985 006
     AOX 3                           0.1 – 3.0 mg/l AOX               0.01 – 0.30 mg/l AOX         470 nm        20       985 007
     BOD5 (in Winkler bottles accor- 2 – 3000 mg/l O2                                              436 nm       25–50     985 822
     ding to DIN EN 1899-1-H51)
     BOD5-TT (tube test)             2 – 3000 mg/l O2                                              436 nm        22       985 825
     Cadmium 2 1)                    0.05 – 2.00 mg/l Cd2+                                         520 nm       10–19     985 014
     Carbonate hardness 15           1.0 – 15.0 °d                    0.4 – 5.4 mmol/l H+        436/585 nm      20       985 015
     Chloride 200                    5 – 200 mg/l Cl–                                              470 nm        20       985 019
     Chloride 50                     0.5 – 50.0 mg/l Cl–                                           470 nm        20       985 021
     Chlorine/Ozone 2                0.05 – 2.50 mg/l Cl2             0.05 – 2.00 mg/l O3          540 nm        20       985 017
     Chlorine dioxide 5              0.2 – 5.0 mg/l ClO2                                           540 nm        20       985 018
     Chromate 5                      0.05 – 2.00 mg/l Cr(VI)          0.1 – 4.0 mg/l CrO42–        540 nm        20       985 024
                                 2)  0.005 – 0.500 mg/l Cr(VI)        0.01 – 1.00 mg/l CrO42–
     COD 40                          2 – 40 mg/l O2                                                345 nm        20       985 027
     COD 60                          5 – 60 mg/l O2                                                345 nm        20       985 022
     COD 160                         15 – 160 mg/l O2                                              436 nm        20       985 026
     COD 160 Hg-free                 15 – 160 mg/l O2                                              436 nm        20       963 026
     COD 300                         50 – 300 mg/l O2                                              436 nm        20       985 033
     COD 1500                        100 – 1500 mg/l O2                                            620 nm        20       985 029
     COD 10000                       1.00 – 10.00 g/l O2                                           620 nm        20       985 023
     COD 15000                       1.0 – 15.0 g/l O2                                             620 nm        20       985 028
     org. Complexing agents 10 0.5 – 10.0 mg/l IBiK                                                540 nm       10–19     985 052
     (screening test)
     Copper 7                        0.10 – 7.00 mg/l Cu2+                                         585 nm         20      985 054
     Cyanide 08                      0.01 – 0.80 mg/l CN–        2)   0.002 – 0.100 mg/l CN–     585/605 nm       20      985 031
     DEHA 1                          0.05 – 1.00 mg/l DEHA                                         540 nm         20      985 035
     (Diethylhydroxylamine)
     Ethanol 1000                    0.10 – 1.00 g/l EtOH             0.013 – 0.130 Vol.% EtOH     620 nm         23      985 838
     Fatty acids see Organic acids
     Fluoride 2                      0.1 – 2.0 mg/l F–                                             620 nm         20      985 040
     Formaldehyde 8                  0.1 – 8.0 mg/l HCHO                                           585 nm         20      985 041
     1) This test cannot be evaluated with the NANOCOLOR ® 250 D 2)           a more sensitive measuring range is possible by using
     50 mm semi-micro cuvettes (Cat. No. 919 50) 3) without bar code



72    MN                                                                                                               www.mn-net.com
                                    The system for photometric water analysis

NANOCOLOR ® tube tests

Test                            Measuring ranges for NANOCOLOR ® photometers             Wavelength     No. of    Cat. No.
                                                                                                        tests
Hardness 20                   1.0 – 20.0 °d                    5 – 50 mg/l Mg2+            540 nm        20      985 043
                              0.2 – 3.6 mmol/l                10 – 100 mg/l Ca2+
residual Hardness 1           0.02 – 1.00 °d                  0.004 – 0.180 mmol/l         540 nm         20     985 084
HC 300                        0.5 – 5.6 mg/l KW               30 – 300 mg/kg KW            436 nm         20     985 057
(hydrocarbons)
Iron 3                      0.10 – 3.00 mg/l Fe          2)   0.02 – 1.00 mg/l Fe          540 nm         20     985 037
Lead 5 1)                   0.10 – 5.00 mg/l Pb2+                                          520 nm         20     985 009
Manganese 10                0.1 – 10.0 mg/l Mn           2)   0.02 – 2.00 mg/l Mn          470 nm         20     985 058
Methanol 15                 0.2 – 15.0 mg/l MeOH                                           620 nm         23     985 859
Molybdenum 40               1.0 – 40.0 mg/l Mo(VI)            1.6 – 65.0 mg/l MoO42–     345/365 nm       20     985 056
Nickel 7                    0.10 – 7.00 mg/l Ni2+        2)   0.02 – 1.00 mg/l Ni2+        470 nm         20     985 061
Nitrate 50                  0.3 – 22.0 mg/l NO3-N             2 – 100 mg/l NO3–          365/385 nm       20     985 064
Nitrate 250                 4 – 60 mg/l NO3-N                 20 – 250 mg/l NO3–         365/385 nm       20     985 066
Nitrite 2                   0.003 – 0.460 mg/l NO2-N          0.02 – 1.50 mg/l NO2–        540 nm         20     985 068
Nitrite 4              NEW! 0.1 – 4.0 mg/l NO2-N              0.3 – 13.0 mg/l NO2–         540 nm         20     985 069
total Nitrogen TNb 22       0.5 – 22.0 mg/l N                                            365/385 nm       20     985 083
total Nitrogen TNb 220 NEW! 5 – 220 mg/l N                                               365/385 nm       20     985 088
nonionic Surfactants, see Surfactants
Organic acids 3000 NEW! 30 – 3000 mg/l CH3COOH                0.5 – 50.0 mmol/l            470 nm         20     985 050
                                                              CH3COOH
Ozone, see Chlorine/Ozone 2
Peroxide 2                   0.03 – 2.00 mg/l H2O2                                         620 nm       10–19    985 871
pH 6.5–8.2                   pH 6.5 – 8.2                                                 436/540 nm     100     918 72 3)
Phenolic index 5 1)          0.2 – 5.0 mg/l                                                520 nm        20      985 074
ortho- and                   0.05 – 1.50 mg/l PO4-P           0.2 – 5.0 mg/l PO43–         690 nm        19      985 076
total Phosphate 1         2) 0.010 – 0.800 mg/l PO4-P         0.03 – 2.50 mg/l PO43–
ortho- and                   0.20 – 5.00 mg/l PO4-P           0.5 – 15.0 mg/l PO43–        690 nm         19     985 081
total Phosphate 5
ortho- and                   0.30 – 15.00 mg/l PO4-P          1.0 – 45.0 mg/l PO43–        690 nm         19     985 080
total Phosphate 15
ortho- and                   5.0 – 50.0 mg/l PO4-P            15 – 150 mg/l PO43–          690 nm         19     985 055
total-Phosphate 45     NEW!
ortho- and                   10.0 – 50.0 mg/l PO4-P           30 – 150 mg/l PO43–          436 nm         19     985 079
total Phosphate 50
POC 200                NEW! 20 – 200 mg/l                     1.0 – 40.0 mg/l              436 nm         20     985 070
(polyoxycarboxylic acids)
Potassium 50                 2 – 50 mg/l K+                                                690 nm         20     985 045
Oxygen 12                    0.5 – 12.0 mg/l O2                                            436 nm         22     985 082
Starch 100                   5 – 100 mg/l starch                                           540 nm         19     985 085
Sulphate 200                 10 – 200 mg/l SO42–                                           436 nm         20     985 086
Sulphate 1000                200 – 1000 mg/l SO42–                                         436 nm         20     985 087
Sulphite 10                  0.2 – 10.0 mg/l SO32–       2)   0.05 – 2.40 mg/l SO32–       436 nm         20     985 089
Sulphite 100                 5 – 100 mg/l SO3  2–                                          470 nm         19     985 090
nonionic Surfactants 15      0.3 – 15.0 mg/l Triton® X-100                               610/620 nm       20     985 047
                       NEW!
Thiocyanate 50               0.5 – 50.0 mg/l SCN–                                          470 nm         20     985 091
Tin 3 1)                     0.10 – 3.00 mg/l Sn                                           520 nm         18     985 097
TOC 70                       2 – 70 mg/l TOC                                               585 nm         10     985 094
TTC / Sludge activity 150    5 – 150 µg TPF                   0.050 – 2.300 E              470 nm         20     985 890
Zinc 4                       0.10 – 4.00 mg/l Zn2+                                         620 nm         20     985 096
1) This test cannot be evaluated with the NANOCOLOR ® 250 D 2)        a more sensitive measuring range is possible by using
50 mm semi-micro cuvettes (Cat. No. 919 50) 3) without bar code
Special chemicals for NANOCOLOR ® standard tests are described with the individual parameters and tests from page 76 and on
page 97.



www.mn-net.com                                                                                                         MN     73
                    The system for photometric water analysis

     NANOCOLOR ® standard tests

     The perfect tool for routine analysis of drinking water, surface water and boiler feed water
          Ready to use reagents in 100 ml bottles
          Exact sample dosage in volumetric flasks
          Measurement in 10 or 50 mm cuvettes
          Variable measuring ranges due to different cuvette sizes
          Extreme sensitivity due to large cuvette size
          Outstanding accuracy due to large sample volume
          Easy dilutions




     NANOCOLOR ® standard tests · product information for photometers 500 D, 400 D, Linus and 300 D
     These tests cannot be evaluated with the photometer NANOCOLOR® 350 D and 250 D. However, they can still be used with the
     photometers NANOCOLOR ® SM-1, 200 D, 100 D, 50 D, 25, PT-2 and PT-3.

                    Test                  Measuring ranges NANOCOLOR ® photometers                Wave-        No. of     Cat. No.
                                                                                                  length      tests 2)
     Aluminium                  0.01 – 1.00 mg/l Al3+                                             540 nm        200       918 02
     Ammonium                   0.01 – 2.0 mg/l NH4-N                0.01 – 2.5 mg/l NH4+         690 nm        100       918 05
     Chloride                   0.2 – 125 mg/l Cl–                                                470 nm        220       918 20
     Chlorine                   0.02 – 10.0 mg/l Cl2                                              540 nm        250       918 16
     Chlorine dioxide           0.04 – 4.00 mg/l ClO2                                             540 nm         50       918 163
     Chromate                   0.01 – 3.0 mg/l Cr(VI)               0.01 – 6.0 mg/l CrO42–       540 nm        250       918 25
     Cobalt                     0.002 – 0.70 mg/l Co2+                                            540 nm        220       918 51
     Colour (Hazen/DIN) 1)      5 – 500 mg/l Pt (Hazen)              0.2 – 20.0 1/m               436 nm         –       method 1-39
     Copper 4)                  0.01 – 10.0 mg/l Cu2+                                             585 nm        250       918 53
     Cyanide                    0.001 – 0.50 mg/l CN–                                             585 nm        250       918 30
     Fluoride              NEW! 0.05 – 2.00 mg/l F–                                               585 nm        200       918 142
     Hydrazine                  0.002 – 1.50 mg/l N2H4                                            436 nm        220       918 44
     Iron 4)                    0.01 – 15.0 mg/l Fe                                               470 nm        250       918 36
     Manganese 4)               0.01 – 10.0 mg/l Mn                                               470 nm        250       918 60
     Nickel  4)                 0.01 – 10.0 mg/l Ni2+                                             436 nm        250       918 62
     Nitrate                    0.9 – 30.0 mg/l NO3-N                2 – 140 mg/l NO3–          365/385 nm      100       918 65
     Nitrate Z                  0.02 – 1.0 mg/l NO3-N                0.1 – 5.0 mg/l NO3–          520 nm        440       918 63
     Nitrite 4)                 0.002 – 0.30 mg/l NO2-N              0.005 – 1.00 mg/l NO2–       520 nm        220       918 67
     Phenol                     0.01 – 7.0 mg/l Phenol                                            470 nm        440       918 75
     ortho-Phosphate            0.04 – 6.5 mg/l PO4-P                0.1 – 20.0 mg/l PO4 3–       690 nm        440       918 77
     ortho-Phosphate 4)         0.2 – 17 mg/l PO4-P                  0.5 – 50 mg/l PO43–          436 nm        440       918 78
     Silica 4)                  0.01 – 5.00 mg/l Si                  0.02 – 10.0 mg/l SiO2        690 nm        250       918 48
                                0.002 – 0.100 mg/l Si 3)             0.005 – 0.200 mg/l SiO2 3)   800 nm
     Sulphide                   0.01 – 3.0 mg/l S2–                                             620/660 nm      250       918 88
     Turbidity (formazine/DIN)1 1 – 100 TE/F (= FAU)                 0.5 – 40.0 1/m             620/860 nm       –       method 1-93
     Zinc                       0.02 – 3.0 mg/l Zn2+                                              620 nm        250       918 95
     1) A NANOCOLOR ® standard test is not required for this test. The original sample is to be measured without additional reagents.
     2) Maximum number; the actual number of tests depends on the sample volume.
     3) High sensitivity measurement 4) Simplified procedure in a beaker is possible. Please ask for special instructions!

     Special chemicals for NANOCOLOR ® standard tests are described with the individual parameters and tests from page 76.



74     MN                                                                                                             www.mn-net.com
                                       The system for photometric water analysis

NANOCOLOR ® standard tests · extraction methods
Extraction methods are a special type of standard tests:
Some analytical procedures require application of two immis-
cible phases. By shaking in a separation funnel the colour
complex is transferred into the organic phase.
This method is used:
   to increase sensitivity when the colour intensity is higher
   in the organic solvent.
   to increase selectivity, i. e. only the colour complex of
   the substance in question is soluble in the organic phase
   while interfering compounds remain in the aqueous
   phase.
   when the colour complex formed in the reaction is not
   soluble in water.
The NANOCOLOR ® analytical system mainly uses chlorinat-
ed hydrocarbons as organic phase which form the lower layer
during extraction. Some analytical instructions prescribe two
consecutive extractions to increase selectivity and eliminate
interferences.



NANOCOLOR ® standard tests with extraction methods
These tests cannot be evaluated with the photometer NANOCOLOR® 350 D and 250 D. The reagent sets 918 10, 918 13, 918 32
and 918 34 contain chlorinated hydrocarbons. If national or international legal limitations for the use, storage or shipment of tetra-
chloromethane must be observed, the reagent sets 918 10 and 918 13 are also available without tetrachloromethane.

 Test                                              Range                                        Wave-        No. of       Cat. No.
                                           NANOCOLOR ® 500 D, 400 D, Linus, 300 D               length       tests
 Cadmium                                       0.002 – 0.50 mg/l Cd2+                           520 nm        25          918 13
 Cadmium (without tetrachlorometh-             0.002 – 0.50 mg/l Cd2+                           520 nm        25          918 131
 ane) *
 Detergents anionic                             0.02 – 5.0 mg/l MBAS                        620 nm             40         918 32
 Detergents cationic                            0.05 – 5.0 mg/l CTAB                        436 nm             40         918 34
 Lead                                           0.005 – 1.00 mg/l Pb2+                      520 nm             25         918 10
 Lead (without tetrachloromethane) *            0.005 – 1.00 mg/l Pb2+                      520 nm             25         918 101
 * The tetrachloromethane required for these tests has to be purchased from a local laboratory shop.

Special chemicals for these NANOCOLOR ® tests are described with
the individual parameters and tests from page 76.




Accessories for NANOCOLOR ® standard tests
 Description                                                                                                Pack of       Cat. No.
 Precision cuvettes for NANOCOLOR® standard test
 Glass cuvettes, 10 mm optical path                                                                             2          919 33
 Lids for glass cuvettes 10 mm                                                                                  2          919 41
 Glass cuvette, 50 mm optical path                                                                              1          919 35
 Semi-micro cuvette 50 mm for reduced analytical preparations and sensitive phosphate measure-                  1          919 50
 ments
 Lids for glass cuvettes 50 mm                                                                                 2           919 40
 Disposable cuvettes, plastic, 10 mm optical path                                                             100          919 37
 Accessories for extraction methods
 Separation funnel 100 ml, glass, with NS glass tap and polyethylene stopper                                    2          916 64
 Stand with clamps and bosses for 4 separation funnels, height 70 cm                                            1          916 95

www.mn-net.com                                                                                                                  MN       75
                    The system for photometric water analysis

     Description of individual parameters and tests

     Alcohol                                                            NANOCOLOR ® Ammonium 10                      Cat. No. 985 004
     see Ethanol and Methanol                                           Type:                    tube test 0-04
                                                                        Measuring range:         0.2 – 8.0 mg/l NH4-N
     Alkalinity (p/m)                                                   Shelf life:              at least 1 year at 15 – 25 °C
     see Carbonate hardness                                             Sea water suitability:   yes
                                                                        NANOCOLOR ® Ammonium 50                      Cat. No. 985 005
     Aluminium                                                  Al      Type:                    tube test 0-05
                                                                        Measuring range:         1 – 40 mg/l NH4-N
     Reaction basis:
     In weakly acidic solution aluminium ions react with erio-          Shelf life:              at least 1 year at 15 – 25 °C
     chrome cyanine R to form a red-violet coloured complex.            Sea water suitability:   yes
     Strongly acidic and buffered samples have to be adjusted to        NANOCOLOR ® Ammonium 200                     Cat. No. 985 006
     pH 6. Turbid solutions have to be filtered (membrane filters
     0.45 µm, Cat. No. 916 50). Fluoride interferes.                    Type:                    tube test 0-06
                                                                        Measuring range:         30 – 160 mg/l NH4-N
     NANOCOLOR ® Aluminium 07                     Cat. No. 985 098      Shelf life:              at least 1 year at 15 – 25 °C
     Type:                    tube test 0-98                            Sea water suitability:   yes
     Measuring range:         0.02 – 0.70 mg/l Al3+
     Shelf life:              at least 1 year                           NANOCOLOR ® Ammonium                           Cat. No. 918 05
     Sea water suitability:   yes                                       Type:                    standard test 1-05
                                                                        Measuring range:         0.01 – 2.0 mg/l NH4-N
     NANOCOLOR ® Aluminium                            Cat. No. 918 02
                                                                        Shelf life:              at least 1 year at 15 – 25 °C
     Type:                    standard test 1-02                        Sea water suitability:   no
     Measuring range:         0.01 – 1.00 mg/l Al3+
     Shelf life:              at least 2 years
     Sea water suitability:   yes                                       AOX                                                         AOX

                                                                        DIN method: Adsorbable organically bound halogens (AOX)
                                                                        is an important sum parameter for the control of water quality.
                                                                        The AOX content represents the sum of organically bound
                                                                        halogens (chlorine, bromine, iodine) which are adsorbable to
                                                                        a suitable adsorbent.
                                                                        The pH-value of the sample must be between 3 and 5.
                                                                        NANOCOLOR ® AOX 3                            Cat. No. 985 007
                                                                        Type:                   tube test 0-07
                                                                        Measuring range:        0.1 – 3.0 mg/l AOX
                                                                                                0.01 – 0.3 mg/l AOX
                                                                        Shelf life:             at least 1 year
                                                                        Sea water suitability: yes, with 200 ml of rinsing solution
                                                                        For further information about AOX see page 98.




                                                                        BOD5                                                        BOD
     Ammonium                                                  NH4
                                                                        NEW! Simple determination of the biochemical oxygen de-
     Reaction basis:                                                    mand after 5 days (BOD5) of undiluted samples without using
     DIN method: At a pH value of about 12.6 ammonium ions re-          a control in accordance with DIN EN 1899-2-H52! The oxygen
     act with hypochlorite and salicylate in the presence of sodium     enriched, undiluted sample is incubated in test tubes for 5
     nitroprussiate as catalyst to form a blue indophenol.              days at 20 ± 1°C in the dark. Determination of the dissolved
     Good reproducibility is obtained for weakly polluted waters.       oxygen after 5 days is based on the Winkler procedure, DIN
     Heavy pollution causes errors, unless a distillation precedes      EN 25813-G21.
     the analysis. Strongly acidic and buffered samples have to be
     adjusted to pH 9 – 10 for the test using sodium hydroxide so-      NANOCOLOR ® BOD5                             Cat. No. 985 822
     lution.
                                                                        Type:                    in Winkler bottles, according to
     NANOCOLOR ® Ammonium 3                       Cat. No. 985 003                               DIN EN 1899-1-H51; test 8-22
     Type:                    tube test 0-03                            Measuring range:         2 – 3000 mg/l O2
     Measuring range:         0.04 – 2.30 mg/l NH4-N                    Shelf life:              at least 2 years
     Shelf life:              at least 1 year at 15 – 25 °C             Sea water suitability:   yes
     Sea water suitability:   yes, after dilution (1:1).

76     MN                                                                                                              www.mn-net.com
                                      The system for photometric water analysis

Description of individual parameters and tests
                                                                 Type:                  standard test 1-13
                                                                 Measuring range:       0.002 – 0.50 mg/l Cd2+
                                                                 Reaction basis:        (b) dithizone method
                                                                 Shelf life:            at least 18 months
                                                                 Sea water suitability: no
                                                                 Reagent set 918 13 contains tetrachloromethane. Observe
                                                                 local regulations concerning halogenated hydrocarbons.
                                                                 Reagent set 918 131 does not contain the required tetrachlo-
                                                                 romethane which has to be purchased separately from a local
                                                                 laboratory store.

                                                                 NANOCOLOR ® Cadmium KS                       Cat. No. 918 132

NANOCOLOR ® BOD5-TT                         Cat. No. 985 825     Type:                   Pre-extraction kit for the determination
Shelf life: at least 2 years                                                             of cadmium in sludge, test 1-132
Type:                     tube test 8-25                         Shelf life:             at least 18 months
Measuring range:          2 – 3000 mg/l O2                       sufficient for:         25 pre-extractions
Shelf life:               at least 2 years                       This test contains chloroform. Observe local regulations con-
Sea water suitability: yes                                       cerning halogenated hydrocarbons.
For further information about BOD5, please see page 99.

                                                                 Carbonate hardness                                        CO3

Bromine                                                 Br2      Photometric determination is performed with bromophenol
                                                                 blue.
Bromine and bromating reagents such as 1,3-dibromo-5,5-
dimethylhydantoin (DBH) are used – like chlorine – for disin-    NANOCOLOR ® Carbonate hardness 15 Cat. No. 985 015
fecting swimming pool water.                                     Type:                  tube test 0-15
For determination of bromine you may use all                     Measuring range:       1.0 – 15.0 °d
NANOCOLOR® Chlorine tests (see page 83). A factor for                                   0.4 – 5.4 mmol/l H+
conversion is given in the instructions.                         Shelf life:            at least 1 year
                                                                 Sea water suitability: yes



Cadmium                                                  Cd
                                                                 Chloride                                                  Cl–
Reaction bases:                                                  Reaction basis:
(a) Cadion method: in alkaline solution cadmium ions react       Chloride ions react with mercury(II) thiocyanate to form undis-
with cadion [1-(4-nitrophenyl)-3-(4-phenylazophenyl)-tria-       sociated mercury(II) chloride. The liberated thiocyanate forms
zene] to form a red colour complex, which is evaluated pho-      with iron(III) ions a blood-red colouration.
tometrically.
                                                                 Thiocyanate, sulphide, thiosulphate, bromide and iodide in-
(b) Dithizone method: at a pH > 6 cadmium ions react with        terfere, because they react like chloride.
dithizone to form primary cadmium dithizonate, which is sta-
ble in strongly alkaline medium and dissolves in carbon tetra-   NANOCOLOR ® Chloride 200                     Cat. No. 985 019
chloride (tetrachloromethane) highly sensitive with pink         Type:                  tube test 0-19
colour. Interfering heavy metals are removed with dithizone in   Measuring range:       5 – 200 mg/l Cl–
acidic medium.
                                                                 Shelf life:            at least 1 year
Strongly alkaline and buffered sample solutions have to be       Sea water suitability: yes, after dilution 1:200
adjusted to pH 2 for the test.
                                                                 NANOCOLOR ® Chloride 50                      Cat. No. 985 021
NANOCOLOR ® Cadmium 2                       Cat. No. 985 014     Type:                  tube test 0-21
Type:                    tube test 0-14                          Measuring range:       0.5 – 50 mg/l Cl–
Measuring range:         0.10 – 2.00 mg/l Cd2+                   Shelf life:            at least 1 year
Reaction basis:          (a) cadion method                       Sea water suitability: no
Shelf life:              at least 1 year                         NANOCOLOR ® Chloride                          Cat. No. 918 20
Sea water suitability:   yes                                     Type:                  standard test 1-20
                                                                 Measuring range:       0.2 – 125 mg/l Cl–
NANOCOLOR ® Cadmium
                                                                 Shelf life:            at least 1 year
with tetrachloromethane                      Cat. No. 918 13     Sea water suitability: no
without tetrachloromethane                  Cat. No. 918 131


www.mn-net.com                                                                                                            MN        77
                    The system for photometric water analysis

     Description of individual parameters and tests
                                                                        Chlorine dioxide, like chlorine, reacts with DPD to form a red-
                                                                        violet dye. For a simultaneous determination of chlorine, chlo-
     Chlorine / Chlorine dioxide / Ozone                        Cl2     rine dioxide and chlorite we recommend the procedure Test
                                                                        1-164. For an evaluation in accordance with drinking water
     Reaction basis:                                                    specifications only the 50-mm rectangular cuvette can be ap-
     DIN EN ISO method: Free chlorine, total chlorine and ozone         plied.
     react with DPD (N,N-diethyl-1,4-phenylene diamine) to form
     a red-violet dye. By defined addition of iodide ions one can
     differentiate between the individual components.
     Turbidities caused by suspended matter are removed by cen-
     trifugation of the sample. Oxidising compounds like bromine,       Chromium / Chromate                                       Cr
     bromoamines, iodine and higher-valent manganese com-
                                                                        Reaction basis:
     pounds simulate chlorine. When the chlorine concentration
                                                                        In acidic medium, chromate ions react with diphenylcarbazide
     exceeds 20 mg/l the red dye can be bleached (low test re-
                                                                        (lyophilised in the tube test) to form a red-violet colour com-
     sults).
                                                                        plex. Chromium(III) ions are not determined unless they are
     NANOCOLOR ® Chlorine / Ozone 2                  Cat. No. 985 017   converted to Cr(VI) by silver-catalysed oxidation with ammo-
                                                                        nium peroxodisulphate/sulphuric acid.
     Type:                    tube test 0-17
                                                                        For the determination of chromium in electroplating baths
     Measuring range:         0.05 – 2.5 mg/l Cl2
                                                                        please ask for our special instructions.
                              0.05 – 2.0 mg/l O3
     Shelf life:              at least 1 year                           NANOCOLOR ® Chromate 5                      Cat. No. 985 024
     Sea water suitability:   yes                                       Type:                    tube test 0-24
                                                                        Measuring range:         0.1 – 4.0 mg/l CrO42–
                                                                                                 0.01 – 1.00 mg/l CrO42– when using
                                                                                                 semi-micro cuvettes 50 mm
                                                                                                 (Cat. No. 919 50)
                                                                        Shelf life:              at least 2 years
                                                                        Sea water suitability:   yes
                                                                        NANOCOLOR ® Chromate                          Cat. No. 918 25
                                                                        Type:                  standard test 1-25
                                                                        Measuring range:       0.01 – 6.0 mg/l CrO42–
                                                                        Shelf life:            at least 2 years
                                                                        Sea water suitability: yes
                                                                        If you need to determine total chromium with the above rea-
                                                                        gent sets chromate, you require:
                                                                        NanOx metal                               Cat. No. 918 978
                                                                        + empty reaction tubes 14 mm ID               Cat. No. 916 80


     NANOCOLOR ® Chlorine                             Cat. No. 918 16
     Type:                    standard test 1-16
     Measuring range:         0.02 – 10.0 mg/l Cl2
                                                                        Cobalt                                                    Co
     Shelf life:              at least 1 year                           Reaction basis:
     Sea water suitability:   yes                                       At a pH > 5 cobalt(II) ions react with 4-[5-chloropyridyl-(2)-
                                                                        azo]-m-phenylene diamine (5-Cl-PADAB) to form a pink col-
     NANOCOLOR ® Chlorine dioxide 5                  Cat. No. 985 018
                                                                        our complex which, contrary to other heavy metal complexes,
     Type:                    tube test 0-18                            is also stable below pH 5.
     Measuring range:         0.2 – 5.0 mg/l ClO2                       Strongly acidic and buffered samples have to be adjusted to
     Shelf life:              at least 1 year                           pH 6 for the test.
     Sea water suitability: no
                                                                        NANOCOLOR ® Cobalt                            Cat. No. 918 51
     Chlorine dioxide, like chlorine, reacts with DPD to form a red-
     violet dye. By using a special supplementary reagent, chlo-        Type:                    standard test 1-51
     rine dioxide is determined selectively.                            Measuring range:         0.002 – 0.70 mg/l Co2+
                                                                        Shelf life:              at least 2 years
     NANOCOLOR ® Chlorine dioxide                    Cat. No. 918 163
                                                                        Sea water suitability:   yes
     Type:                    standard test 1-163
     Measuring range:         0.04 – 4.00 mg/l ClO2
     Shelf life:              at least 18 months
     Sea water suitability:   yes




78     MN                                                                                                             www.mn-net.com
                                        The system for photometric water analysis

Description of individual parameters and tests

                                                                 NANOCOLOR ® COD 300                          Cat. No. 985 033

COD (chemical oxygen demand)                             COD     Type:                      tube test 0-33
                                                                 Measuring range:           50 – 300 mg/l O2
Reaction basis:                                                  Shelf life:                at least 1 year at 15 – 25 °C
ISO method: The chemical oxygen demand of a water is de-         Sea water suitability: no
termined by silver-catalysed oxidation with potassium dichro-
mate/sulphuric acid at 148 °C during a two hour period.          This test uses the same, 10fold reduced chemical preparation
                                                                 as the German Standard Methods (DIN) determination. Com-
Using different chromate concentrations one can cover differ-    parability of test results is thus best for this tube test.
ent measuring ranges. For COD 40/160/300 the colour de-
crease of the yellow dichromate ions is measured, for COD        NANOCOLOR ® COD 1500                         Cat. No. 985 029
1500/15000 the colour increase of the green chromium(III)
ions formed is evaluated.                                        Type:                    tube test 0-29
If the chloride content is above 1500 mg/l the sample has to     Measuring range:         100 – 1500 mg/l O2
be diluted with COD-free water, or you may use the chloride      Shelf life:              at least 1 year at 15 – 25 °C
complexing agent. Lower chloride concentrations are masked       Sea water suitability:   no
by the mercury(II) sulphate present in the test tubes.

Tests 985 027, 985 022, 985 026 and 985 029 are in
accordance with ISO 15705:2002
NANOCOLOR ® COD 40                            Cat. No. 985 027
Type:                    tube test 0-27
Measuring range:         2 – 40 mg/l O2
Shelf life:              at least 1 year at 4 – 8 °C
Sea water suitability:   no

NANOCOLOR ® COD 60                            Cat. No. 985 022
Type:                    tube test 0-22
Measuring range:         5 – 60 mg/l O2
Shelf life:              at least 1 year at 4 – 8 °C
Sea water suitability: no
The high COD sensitivity of test 0-27 and test 0-22 requires a
reduced potassium dichromate concentration in the tube test,
resulting in a reduced oxidation potential. This can cause low
results for the "residual COD" (often the substances which       NANOCOLOR ® COD 10000                        Cat. No. 985 023
are most difficult to degrade).
                                                                 Type:                    tube test 0-23
NANOCOLOR ® COD 160                           Cat. No. 985 026   Measuring range:         1.00 – 10.00 g/l O2
Type:                    tube test 0-26                          Shelf life:              at least 1 year at 15 – 25 °C
Measuring range:         15 – 160 mg/l O2                        Sea water suitability:   no
Shelf life:              at least 1 year at 15 – 20 °C           NANOCOLOR ® COD 15000                        Cat. No. 985 028
Sea water suitability:   no
                                                                 Type:                    tube test 0-28
NANOCOLOR ® COD 160 Hg-free                   Cat. No. 963 026   Measuring range:         1.0 – 15.0 g/l O2
Type:                    tube test 0-26                          Shelf life:              at least 1 year at 15 – 25 °C
Measuring range:         15 – 160 mg/l O2                        Sea water suitability:   no
Shelf life:              at least 1 year at 4 – 8 °C             Chloride complexing agent                    Cat. No. 918 911
Sea water suitability:   no
                                                                 for COD determinations with chloride concentrations from
without use of toxic mercury salts                               1000 to 7000 mg/l Cl–.
Chloride contents below 2000 mg/l are eliminated by a simple
                                                                 Cartridges for chloride elimination          Cat. No. 963 911
filtration pretreatment step using a special cartridge and do
not interfere.                                                   Shelf life: at least 1 year at 4 – 8 °C
For more information concerning this test refer to page 100.     one cartridge is sufficient for removal of up to 2000 mg/l Cl–
                                                                 COD-free water                               Cat. No. 918 993
                                                                 for control measurements and for dilution of water samples.




www.mn-net.com                                                                                                            MN      79
                    The system for photometric water analysis

     Description of individual parameters and tests

                                                                         NANOCOLOR ® Copper 7                         Cat. No. 985 054

     Colour / Colouration                                       1/m      Type:                    tube test 0-54
                                                                         Measuring range:         0.10 – 7.00 mg/l Cu2+
     Colours occurring in natural waters are usually in the yellow       Shelf life:              at least 2 years
     to brown range. Other colours (e. g. in waste water) are not        Sea water suitability:   yes
     completely covered.
     Reaction basis:                                                     NANOCOLOR ® Copper                              Cat. No. 918 53
     For determination of the colour intensity one uses the HAZEN        Type:                    standard test 1-53
     scale, which is calibrated with platinum-cobalt chloride stand-     Measuring range:         0.10 – 7.00 mg/l Cu2+
     ards. In accordance with DIN EN ISO 7887-C1-3 the colour-
     ation is measured as spectral absorption coefficient at 3           Shelf life:              at least 2 years
     wavelengths: 436 nm, ~525 nm, ~620 nm. The colour of the            Sea water suitability:   yes
     pure sample is measured without any reagents.                       Reagents for lime precipitation              Cat. No. 918 939
     NANOCOLOR® photometers are programmed with all neces-
     sary calibration data for the above determinations.                 Reagent for eliminating interfering calcium (up to 20 g/l Ca2+)
                                                                         for copper, nickel and zinc determinations.
     Turbidities have to be filtered (membrane filtration kit, Cat.
     No. 916 50). If you also need the turbidity value of your sam-
     ple, you may calculate it from the difference of a measure-
     ment before and another after filtration.
                                                                         Cyanide                                                   CN–
     NANOCOLOR ®       Colour
                                                                         Reaction basis:
     Type:                    no reagents required; test 1-39            DIN method: Cyanide ions react with chloroamine T to form
     Measuring range:         5 – 500 mg/l Pt                            cyanogen chloride which, with a pyridine derivative and bar-
                              0.2 – 20.0 1/m                             bituric acid forms a polymethine dye. The test measures free
     Sea water suitability:   yes                                        cyanide and cyanide complexes which can be destroyed with
                                                                         chlorine.
                                                                         For determination of cyanide which can be easily liberated
     Complexing agents (organic)                                IBiC     and for determination of total cyanide please ask for a spe-
                                                                         cial instruction from MACHEREY-NAGEL.
     Reaction basis:
     DIN method: Photometric determination of the decolouration          NANOCOLOR ® Cyanide 08                       Cat. No. 985 031
     of the bismuth-xylenol orange complex.
                                                                         Type:                    tube test 0-31
     The complexing agents are determined relative to the bis-           Measuring range:         0.01 – 0.80 mg/l CN–
     muth complexing index IBiC. The following conversion factors                                 0.002 – 0.100 mg/l CN–
     hold true:                                                                                   when using semi-micro cuvettes
     1 mg/l IBiC = 1.4 mg/l EDTA (M = 292 g/mol) = 1.0 mg/l NTA                                   50 mm (Cat. No. 919 50)
     This method is a screening test which covers strong complex-        Shelf life:              at least 1 year
     ing agents. If the result is positive, metals present in the sam-   Sea water suitability:   yes, after dilution 1:4
     ple may be partially or completely withdrawn from the
     photometric determination. For the analysis of metals in this       NANOCOLOR ® Cyanide                             Cat. No. 918 30
     case a decomposition (e. g. with Crack set Cat. No. 918 08 or       Type:                    standard test 1-30
     NanOx Metal Cat. No. 918 978) has to precede the photomet-          Measuring range:         0.001 – 0.5 mg/l CN–
     ric measurement.
                                                                         Shelf life:              at least 1 year
     Copper(I) cyanide simulates the presence of a strong com-           Sea water suitability:   yes
     plexing agent. It can be destroyed by addition of zinc powder.
     NANOCOLOR ® org. Complexing agents 10                               Decomposition
     (screening test)                   Cat. No. 985 052
                                                                         for decomposition see page 91 ff.
     Type:                    tube test 0-52
     Measuring range:         0.5 – 10.0 mg/l IBiK
     Shelf life:              at least 1 year
     Sea water suitability:   yes, after dilution (1:20)                 DEHA (diethylhydroxylamine)                              DEHA

                                                                         In boiler houses the carcinogenic hydrazine is more and more
     Copper                                                     Cu       replaced by diethylhydroxylamine (DEHA) to remove oxygen.
                                                                         Reaction basis:
     In weakly alkaline solution copper(II) ions react with cupri-       Measurement of the reduction properties of DEHA for iron(III)
     zone [oxalic acid bis(cyclohexylidene hydrazide)] to form a         ions and photometric determination or the iron(II) ions formed
     blue complex. Strongly acidic and buffered samples have to          during 15 min heating to 100 °C.
     be adjusted to pH 9 using ammonia. Cr(III) causes low results
                                                                         Interferences caused by iron(II) present in the sample can be
     (eliminated by oxidation to chromate using ammonium perox-
                                                                         eliminated by subtraction of the iron(II) from the iron(II) +
     odisulphate and sulphuric acid).
                                                                         DEHA result.
     For removal of interfering calcium you may use the reagents
     for lime precipitation.

80     MN                                                                                                                 www.mn-net.com
                                       The system for photometric water analysis

Description of individual parameters and tests

NANOCOLOR ® DEHA 1                           Cat. No. 985 035     NANOCOLOR ® Fluoride                           Cat. No. 918 142
Type:                    tube test 0-35                           Type:                    standard test 1-42
Measuring range:         0.05 – 1.00 mg/l DEHA                    Measuring range:         0.05 – 2.00 mg/l F–
Shelf life:              at least 1 year                          Reaction basis:          (b) SPADNS method
                                                                                                                        NEW!
Sea water suitability:   yes                                      Shelf life:              at least 18 months
                                                                  Sea water suitability:   yes, after distillation
Detergents see Surfactants


                                                                  Formaldehyde                                             HCHO
Ethanol                                                    EtOH
                                                                  Formaldehyde serves as basic material for resins, as binder
Reaction basis:                                                   for the production of wood-based materials, as textile
The enzyme alcoholoxidase splits ethanol to form acetalde-        auxiliary, as disinfectant and preservative, and as raw mate-
hyde and hydrogen peroxide. The peroxide formed reacts by         rial for many chemical and pharmaceutical products.
catalytic oxidation of a chromogen with peroxidase to a blue      Reaction basis:
dye.                                                              Formaldehyde reacts with chromotropic acid in sulphuric acid
Applicable for spirits, beer and fruit juices. Strong oxidising   solution to form a violet dye.
agents can cause high results.                                    NANOCOLOR ® Formaldehyde 8                     Cat. No. 985 041
NANOCOLOR ®       Ethanol 1000               Cat. No. 985 838     Type:                    tube test 0-41
Type:                    tube test 8-38                           Measuring range:         0.1 – 8.0 mg/l HCHO
Measuring range:         0.10 – 1.00 g/l EtOH                     Shelf life:              at least 2 years
                         0.013 – 0.13 Vol.% EtOH                  Sea water suitability:   no
Shelf life:              at least 2 years (below < 0 °C)
Sea water suitability:   no
                                                                  total Hardness                                            °d

                                                                  Reaction basis:
                                                                  Photometric determination of total hardness with phthalein
Fluoride                                                   F–     purple.
Reaction basis:
(a) Fluoride shifts the colour of the lanthanum-alizarin com-
plex to violet. In a buffered solution the colour shift can be
measured photometrically.
(b) Photometric determination of fluoride with SPADNS
(1.8-dihydroxy-2-(4-sulfophenylazo)naphthalen-3.6-disul-
fonacid) at 585 nm.
NANOCOLOR ® Fluoride 2                        Cat. No. 918 40
Type:                    tube test 0-40
Reaction basis:          (a) Method with lanthanum-alizarin
                         complex
Measuring range:         0.1 – 2.0 mg/l F–
Shelf life:              at least 18 months
Sea water suitability:   yes, after dilution 1:10
                                                                  NANOCOLOR ® Hardness 20                        Cat. No. 985 043
                                                                  Type:                  tube test 0-43
                                                                  Measuring range:       1.0 – 20.0 °d
                                                                                         5 – 50 mg/l Mg2+
                                                                                         10 – 100 mg/l Ca2+
                                                                  Shelf life:            at least 18 months
                                                                  Sea water suitability: yes, after dilution 1:30
                                                                  With the aid of a selective masking agent differenciation
                                                                  between calcium and magnesium is possible.
                                                                  NANOCOLOR ® residual Hardness 1                Cat. No. 985 084
                                                                  Type:                    tube test 0-84
                                                                  Measuring range:         0.02 – 1.00 °d
                                                                                           0.004 – 0.180 mmol/l
                                                                  Shelf life:              at least 1 year
                                                                  Sea water suitability:   no

www.mn-net.com                                                                                                             MN       81
                    The system for photometric water analysis

     Description of individual parameters and tests
                                                                         Complex iron compounds are not determined. They have to be
                                                                         destroyed by oxidation with nitric acid and sulphuric acid prior
     Hydrocarbons                                               HC       to the test.

     Reaction basis:                                                     NANOCOLOR ® Iron 3                           Cat. No. 985 037
     The determination of hydrocarbons is based on a MN-patent-          Type:                    tube test 0-37
     ed method without halogenated solvents. The extracting
                                                                         Measuring range:         0.10 – 3.00 mg/l Fe
     agent is n-pentane. After removal of polar substances, the ex-
                                                                                                  0.02 – 1.00 mg/l Fe
     tracting agent is evaporated and the test tube with the evap-
                                                                                                  when using semi-micro cuvettes
     oration residue is connected with a COD test tube via a
                                                                                                  50 mm (Cat. No. 919 50)
     screwed joint. Finally, the hydrocarbons are oxidised as COD
     and determined photometrically.                                     Reaction basis:          (a) triazine method
                                                                         Shelf life:              at least 1 year
     NANOCOLOR ® HC 300                          Cat. No. 985 057
                                                                         Sea water suitability:   yes
     after extraction from water or soil samples
     Type:                   tube test 0-57
     Measuring range:        0.5 – 5.6 mg/l HC
                             30 – 300 mg/kg HC
     Shelf life:             at least 1 year
     Sea water suitability: yes
     NANOCOLOR ® HC 300                            Cat. No. 918 571
     Extraction kit for water samples
     Shelf life:              at least 1 year
     NANOCOLOR ® HC 300                            Cat. No. 918 572
     Extraction kit for soil samples
     Shelf life:               at least 1 year
     For a detailed description see page 102.



     Hydrazine                                                 N2H4
                                                                         NANOCOLOR ® Iron                               Cat. No. 918 36
     Reaction basis:
     DIN method: In acidic solution hydrazine reacts with 4-             Type:                    standard test 1-36
     dimethylaminobenzaldehyde to form a yellow-orange com-              Measuring range:         0.01 – 15.0 mg/l Fe
     pound.                                                              Reaction basis:          (b) phenanthroline method
     NANOCOLOR ® Hydrazine                      Cat. No. 918 44          Shelf life:              at least 3 years
     Type:                  standard test 1-44                           Sea water suitability:   yes
     Measuring range:       0.002 – 1.50 mg/l N2H4
     Shelf life:            at least 1 year
     Sea water suitability: yes                                          Lead                                                       Pb
                                                                         Reaction bases:
                                                                         (a) PAR method: in the presence of cyanide lead(II) ions re-
     Iron                                                        Fe      act with 4-[pyridyl-(2)-azo]-resorcinol (PAR) to form a red dye.
                                                                         If interfering heavy metals are present, only the red lead com-
     Reaction bases:                                                     plex is destroyed and the colour decrease is measured pho-
     (a) Triazine method: iron(II) ions react with a triazine deriva-    tometrically.
     tive to form a violet colour complex. The NANOFIX reagent           (b) Dithizone method: at pH 7 to 9 in the presence of cyanide
     capsule is required for reduction of Fe(III) to Fe(II) and for pH   lead(II) ions react with dithizone to form primary lead dithizo-
     adjustment.                                                         nate, which is soluble in carbon tetrachloride with high selec-
     (b) DIN method: iron(II) ions react with 1,10-phenanthroline        tivity yielding a pink solution (extraction method).
     to from an orange colour complex. This method determines
     dissolved and easily soluble iron compounds.                        NANOCOLOR ® Lead 5                           Cat. No. 985 009
     For differentiation between total iron and dissolved iron we        Type:                    tube test 0-09
     recommend the following methods:                                    Measuring range:         0.10 – 5.00 mg/l Pb2+
     A: determination of the dissolved iron after filtration with the    Reaction basis:          (a) PAR method
         membrane filtration kit 0.45 µm (Cat. No. 916 50)               Shelf life:              at least 1 year
     B: determination of total iron after decomposition with
                                                                         Sea water suitability:   no
         NanOx metal (Cat. No. 918 978) or with NANOCOLOR®
         Crack Set (Cat. No. 918 08)




82     MN                                                                                                                 www.mn-net.com
                                       The system for photometric water analysis

Description of individual parameters and tests

NANOCOLOR ® Lead
with tetrachloromethane                         Cat. No. 918 10   Molybdenum                                                  Mo
without tetrachloromethane                     Cat. No. 918 101
                                                                  Reaction basis:
Type:                  standard test 1-10
                                                                  Molybdate ions react with thioglycolic acid to form a yellow
Measuring range:       0.005 – 1.00 mg/l Pb2+                     colour complex. Nitrite interferes with the determination and
Reaction basis:        (b) dithizone method                       has to be destroyed with amidosulphuric acid (Cat. No. 918
Shelf life:            at least 18 months                         973) prior to the analysis.
Sea water suitability: no                                         NANOCOLOR ® Molybdenum 40                     Cat. No. 985 056
Reagent set 918 10 contains tetrachloromethane. Observe
local regulations concerning halogenated hydrocarbons.            Type:                    tube test 0-56
Reagent set 918 101 does not contain the required tetrachlo-      Measuring range:         1.0 – 40.0 mg/l Mo(VI)
romethane which has to be purchased separately from a local                                1.6 – 65.0 mg/l MoO42–
laboratory store.                                                 Shelf life:              at least 1 year (stored below 0 °C)
                                                                  Sea water suitability:   no
Lipophilic substances
see Hydrocarbons page 102
                                                                  Nickel                                                      Ni

                                                                  Reaction bases:
                                                                  In alkaline solution nickel ions react with diacetyldioxime after
                                                                  oxidation to form a red-brown dye.
Manganese                                                 Mn
                                                                  Nickel cyanide and nickel cyanocomplexes are not deter-
Reaction basis:                                                   mined. To remove interfering calcium, use the reagents for
In alkaline solution manganese ions react with formaldoxime       lime precipitation.
to form an orange-red complex (according to DIN 38 406-E2).       NANOCOLOR ® Nickel 7                          Cat. No. 985 061
NANOCOLOR ®       Manganese 10                 Cat. No. 985 058   Type:                    tube test 0-61
Type:                    tube test 0-58                           Measuring range:         0.10 – 7.00 mg/l Ni2+
Measuring range:         0.1 – 10.0 mg/l Mn                                                0.02 – 1.00 mg/l Ni2+
                         0.02 – 2.00 mg/l Mn                                               when using semi-micro cuvettes 50 mm
                         when using semi-micro cuvettes 50 mm                              (Cat. No. 919 50)
                         (Cat. No. 919 50)                        Shelf life:              at least 2 years
Shelf life:              at least 18 months                       Sea water suitability:   yes, after dilution 1:10
Sea water suitability:   yes                                      NANOCOLOR ® Nickel                                Cat. No. 918 62
NANOCOLOR ® Manganese                           Cat. No. 918 60   Type:                    standard test 1-62
Type:                    standard test 1-60                       Measuring range:         0.01 – 10.00 mg/l Ni2+
Measuring range:         0.01 – 10.0 mg/l Mn                      Shelf life:              at least 2 years
Shelf life:              at least 3 years                         Sea water suitability:   yes
Sea water suitability:   yes                                      Reagents for precipitation                    Cat. No. 918 939
                                                                  Reagents for elimination of interfering calcium (up to 20 g/l
                                                                  Ca2+) for copper, nickel and zinc determinations.
Methanol                                                MeOH

Reaction basis:
The enzyme alcoholoxidase splits methanol to form formalde-
hyde and hydrogen peroxide. The peroxide reacts by catalytic
oxidation of a chromogen with peroxidase to a blue colour
dye.
Suitable for the analysis of waste water, surface water and
drinking water.
NANOCOLOR ® Methanol 15                        Cat. No. 985 859
Type:                    tube test 8-59
Measuring range:         0.2 – 15.0 mg/l MeOH
Shelf life:              at least 1 year (stored below 0 °C)
Sea water suitability:   no




www.mn-net.com                                                                                                               MN       83
                    The system for photometric water analysis

     Description of individual parameters and tests
                                                                           Amidosulphuric acid                             Cat. No. 918 973
                                                                           for elimination of interfering nitrite
     Nitrate                                                      NO3
                                                                           Cartridges for chlorine elimination             Cat. No. 963 911
     Reaction bases:                                                       Shelf life:              at least 1 year at 4 – 8 °C
     (a) ISO method: in acidic solution nitrate ions react with 2,6-
     dimethylphenol to form 4-nitro-2,6-dimethylphenol, which can          One cartridge is sufficient for removal of up to 2000 mg/l Cl–.
     be evaluated photometrically.
     Applicable for drinking, ground and weakly polluted surface
     water.
     (b) Reduction method: first nitrate ions are reduced to form
                                                                           Nitrite                                                       NO2
     nitrite ions. In acidic solution these nitrite ions react with sul-   Reaction basis:
     phanilic acid and 1-naphthylamine to form a red azo dye.              (a) DIN EN method: nitrite reacts with sulphanilamide and N-
     Nitrite interferes (elimination with amidosulphuric acid).            (1-naphthyl)-ethylene diamine (lyophilised) to form a red-vio-
                                                                           let azo dye.
     NANOCOLOR ® Nitrate 50                         Cat. No. 985 064
                                                                           (b) Sulphanilic acid method: in acidic solution sulphanilic
     Type:                    tube test 0-64                               acid is diazotised with nitrite. The diazonium salt is coupled
     Measuring range:         0.3 – 22.0 mg/l NO3-N                        with 1-naphthylamine to form a red dye.
     Reaction basis:          (a) DIN method
                                                                           NANOCOLOR ® Nitrite 4                           Cat. No. 985 069
     Shelf life:              at least 2 years
     Sea water suitability:   no                                           Type:                     tube test 0-69
                                                                           Measuring range:          0.1 – 4.0 mg/l NO2-N
                                                                           Reaction basis:           (a) DIN EN method            NEW!
                                                                           Shelf life:               at least 18 months
                                                                           Sea water suitability:    yes

                                                                           NANOCOLOR ® Nitrite 2                           Cat. No. 985 068
                                                                           Type:                     tube test 0-68
                                                                           Measuring range:          0.003 – 0.460 mg/l NO2-N
                                                                           Reaction basis:           (a) DIN EN method
                                                                           Shelf life:               at least 18 months
                                                                           Sea water suitability:    yes
                                                                           NANOCOLOR ® Nitrite                              Cat. No. 918 67
                                                                           Type:                     standard test 1-67
                                                                           Measuring range:          0.002 – 0.30 mg/l NO2-N
                                                                           Reaction basis:           (b) sulphanilic acid method
                                                                           Shelf life:               at least 18 months
     NANOCOLOR ® Nitrate 250                        Cat. No. 985 066       Sea water suitability:    yes

     Type:                    tube test 0-66
     Measuring range:         4 – 60 mg/l NO3-N
     Reaction basis:          (a) DIN method
                                                                           Nitrite in cooling lubricants
     Shelf life:              at least 2 years                             The reagents for sample preparation allow to prepare nitrite-
     Sea water suitability:   no                                           containing samples for photometric measurement using a
                                                                           clarification precipitation (Carrez solutions 1+2).
     NANOCOLOR ® Nitrate                              Cat. No. 918 65
                                                                           Reagents for sample preparation
     Type:                    standard test 1-65                           by clarification precipitation                   Cat. No. 918 937
     Measuring range:         0.9 – 30.0 mg/l NO3-N                        Reagents for removal of emulsions, turbidities and colour pri-
     Reaction basis:          (a) DIN method                               or to the test, e. g. for nitrite in cooling lubricants, seepage wa-
     Shelf life:              at least 2 years                             ter from waste deposits etc.
     Sea water suitability:   no
     NANOCOLOR ® Nitrate Z                            Cat. No. 918 63
     Type:                    standard test 1-63
     Measuring range:         0.02 – 1.0 mg/l NO3-N
     Reaction basis:          (b) reduction method
     Shelf life:              at least 18 months
     Sea water suitability:   no




84     MN                                                                                                                    www.mn-net.com
                                        The system for photometric water analysis

Description of individual parameters and tests
                                                                    617 we, Cat. No. 535 018 or membrane filter 0.45 µm, Cat.
                                                                    No. 916 50) or centrifugated.
Nitrogen (total)                                           TNb      Interferences can be avoided by a steam distillation before
                                                                    the photometric determination.
Reaction basis:
DIN EN ISO method: all organic and inorganic nitrogen con-          NANOCOLOR ® Organic acids 3000                Cat. No. 985 050
taining substances are oxidised to nitrate in acidic solution. In
                                                                    Type:                    tube test 0-50
acidic solution nitrate ions react with 2,6-dimethylphenol to
form 4-nitro-2,6-dimethylphenol, which can be evaluated pho-        Measuring range:         30 – 3000 mg/l CH3COOH
tometrically.                                                       Shelf life:              at least 18 months
                                                                    Sea water suitability:   yes                    NEW!
NANOCOLOR ® total Nitrogen 22                 Cat. No. 985 083
Type:                   tube test 0-83
Measuring range:        0.5 – 22.0 mg/l N
Shelf life:             at least 1 year
Sea water suitability: no
Complete with NanOx N decompostion and compensation re-
agents and nitrate test tubes for 20 determinations




                                                                    Oxygen                                                     O2
NANOCOLOR ®       total Nitrogen 220          Cat. No. 985 088      Reaction basis:
Type:                    tube test 0-88                             Oxygen reacts with manganese(II) ions and potassium iodide
Measuring range:         5 – 220 mg/l N                             to form an equivalent amount of iodine, which is measured
                                                                    photometrically.
Shelf life:              at least 1 year        NEW!
Sea water suitability:   no                                         Good reproducibility is obtained when the tube test is filled to
                                                                    the brim (without air bubbles) at the sampling location in order
                                                                    to immediately bind the oxygen by chemical reaction. The
Complete with NanOx N decompostion and compensation re-             photometric method thus provides a true alternative to the
agents and nitrate test tubes for 20 determinations.                measurement with electrometric oxygen electrodes.
                                                                    Iodine-producing and/or iodine-consuming suspended matter
                                                                    can interfere (elimination by precipitation with aluminium hy-
nonionic Surfactants see Surfactants                                droxide: see DIN EN 25 813 appendix A).
                                                                    NANOCOLOR ® Oxygen 12                         Cat. No. 985 082
                                                                    Type:                    tube test 0-82
                                                                    Measuring range:         0.5 – 12.0 mg/l O2
Organic acids                                             HOAc      Shelf life:              at least 2 years
Reaction basis:                                                     Sea water suitability:   yes
The determination of organic acids is carried out in two steps:
1. Esterification of organic acids with ethylene glycol
2. Conversion of the esters to hydroxamic acids, that sub-
   sequently react with iron (III) ions to form red coloured        Ozone                                                      O3
   complexes, which are determined photometrically.                 see Chlorine, page 78
The concentration of organic acids in waters is affected by
 biochemical processes. Therefore, the samples have to be
determined rapidly after sampling.
Turbid solutions have to be filtered before the determination.
Digested sludge must be filtered (e.g. with folded filter MN


www.mn-net.com                                                                                                                MN       85
                    The system for photometric water analysis

     Description of individual parameters and tests


     Peroxide                                                    H2O2     Phosphate                                                  PO4

     Reaction basis:                                                      Reaction bases:
     Peroxides react by catalytic oxidation of a chromogen in the         (a) DIN EN method: ammonium molybdate reacts with ortho-
     presence of peroxidase to form a blue dye.                           phosphate ions to form phosphomolybdic acid. This is re-
     Strong oxidising agents cause high results.                          duced to molybdenum blue.
                                                                          Strongly alkaline or strongly acidic test solutions have to be
     NANOCOLOR ® Peroxide 2                         Cat. No. 985 871      adjusted to pH 3 – 10 for the test. Precipitations after decom-
     Type:                    tube test 8-71                              position can be removed with membrane filters prior to the
     Measuring range:         0.03 – 2.00 mg/l H2O2                       test. Silica interferes and leads to falsely hight results.
     Shelf life:              at least 1 year at 4 – 8 °C                 (b) Vanadate method: orthophosphate ions react with molyb-
     Sea water suitability:   yes                                         date/vanadate to form a yellow phosphate-molybdate-vanad-
                                                                          ate complex.
                                                                          Turbid solutions have to be filtered prior to the analysis of or-
     pH value                                                    pH       thophosphate. Strongly alkaline or strongly acidic sample so-
                                                                          lutions have to be adjusted to pH 3 – 10 for the test.
     Reaction basis:
     Photometric determination of the pH value in water with              NANOCOLOR ® ortho- and total Phosphate 1
     phenol red as indicator in the lyophilised stable NANOFIX                                                Cat. No. 985 076
     dosage form. Therefore an immediate reproducible colour              Type:                    tube test 0-76
     formation is possible.                                               Measuring range:         0.05 – 1.50 mg/l P (PO4-P)
                                                                                                   0.010 – 0.800 mg/l P (PO4-P)
     NANOCOLOR ® pH 6.5 – 8.2                        Cat. No. 918 72                               when using semi-micro cuvettes 50 mm
     Type:                    tube test 0-72                                                       (Cat. No. 919 50)
     Measuring range:         pH 6.5 – 8.2                                Reaction basis:          (a) DIN method
     Shelf life:              at least 18 months                          Shelf life:              at least 1 year
     Sea water suitability:   yes                                         Sea water suitability:   yes (only ortho-P)
                                                                  OH      NANOCOLOR ® ortho- and total Phosphate 5
                                                                                                              Cat. No. 985 081
     Phenol / Phenolic index
                                                                          Type:                    tube test 0-81
     Reaction bases:                                                      Measuring range:         0.20 – 5.00 mg/l P (PO4-P)
     (a) DIN method: photometric determination of phenols and             Reaction basis:          (a) DIN method
     other compounds capable of oxidative coupling, which form            Shelf life:              at least 1 year
     antipyrine dyes with 4-aminoantipyrine.                              Sea water suitability:   yes (only ortho-P)
     Oxidising substances, reducing substances and cyanides in-
     terfere. For turbid waters and sea water analysis an additional
     extraction with isobutyl methyl ketone (MIBK) is necessary.
     (b) Nitroaniline method: phenol reacts with diazotised 4-ni-
     troaniline to form a red dye. The colour can fluctuate between
     yellow, brown and red with other phenols. In addition to phe-
     nol most phenol derivatives are determined, too. For heavily
     polluted waters the phenols should first be separated by
     steam distillation.
     NANOCOLOR ® Phenolic index 5                   Cat. No. 985 074
     Type:                    tube test 0-74
     Measuring range:         0.2 – 5.0 mg/l phenolic index
     Reaction basis:          (a) DIN method
     Shelf life:              at least 18 months
     Sea water suitability:   yes, after extraction
     Isobutyl methyl ketone                         Cat. No. 918 929
     for colour extraction, for the tube test, for difficult sample ma-
     trices                                                               NANOCOLOR ® ortho- and total Phosphate 15
                                                                                                              Cat. No. 985 080
     NANOCOLOR ® Phenol                              Cat. No. 918 75
                                                                          Type:                    tube test 0-80
     Type:                    standard test 1-75
                                                                          Measuring range:         0.30 – 15.00 mg/l P (PO4-P)
     Measuring range:         0.02 – 7.0 mg/l phenol
                                                                          Reaction basis:          (a) DIN method
     Reaction basis:          (b) nitroaniline method
                                                                          Shelf life:              at least 1 year
     Shelf life:              at least 3 years
                                                                          Sea water suitability:   yes (only ortho-P)
     Sea water suitability:   yes, after dilution 1:10



86     MN                                                                                                                 www.mn-net.com
                                        The system for photometric water analysis

Description of individual parameters and tests

NANOCOLOR ® ortho- and total Phosphate 45
                                    Cat. No. 985 055
Type:                    tube test 0-55
                                                                   POC                                                       POC
Measuring range:         5.0 – 50.0 mg/l P (PO4-P)                 Polyoxycarboxylic acids (POC) are copolymers (e.g. from ac-
Reaction basis:          (a) DIN method                            rolein, acrylic acid, hydroxy- acrylic acid or maleic acid) with
Shelf life:              at least 1 year                           the general empirical formula –[CH2-CO]n-OR.
                                                   NEW!            Polyacrylates are polymers based on the esters of acrylic acid
Sea water suitability:   yes (only ortho-P)
                                                                   with the general empirical formula –[CH2-CH-COOR]n-.
                                                                   Polyoxycarboxylic acids and Polyacrylates are substances
                                                                   with outstanding dispersing properties. Therefore, they are
NANOCOLOR ® ortho- and total Phosphate 50                          used in boiler feed and cooling water as reagents for hard-
                                     Cat. No. 985 079
                                                                   ness stabilization, to prevent coating generated by calcium
Type:                    tube test 0-79                            carbonate.
Measuring range:         10.0 – 50.0 mg/l P (PO4-P)
                                                                   NANOCOLOR ® POC 200                            Cat. No. 985 070
Reaction basis:          (b) vanadate method
Shelf life:              at least 3 year                           Type:                    tube test 0-70
Sea water suitability:   yes (only ortho-P)                        Measuring range:         20 – 200 mg/l POC
                                                                   Shelf life:              at least 18 months
NANOCOLOR ® ortho-Phosphate                     Cat. No. 918 77
                                                                   Sea water suitability:   yes, after dilution 1:4
Type:                    standard test 1-77
Measuring range:         0.04 – 6.5 mg/l P (PO4-P)
Reaction basis:          (a) DIN method
Shelf life:              at least 3 years
Sea water suitability:   yes
NANOCOLOR ® ortho-Phosphate                     Cat. No. 918 78
Type:                    standard test 1-78
Measuring range:         0.2 – 17 mg/l P (PO4-P)
Reaction basis:          (b) vanadate method
Shelf life:              at least 3 years
Sea water suitability:   yes

Determination of total phosphorus with NanOx metal
For phosphorus compounds which are difficult to oxidize, we
recommend decomposition with NanOx metal solid reagents.
For details concerning the decomposition with NanOx metal
in a heating block or with microwave please see page 99.
This decomposition is also used, when total phosphorus is to
be determined with standard tests 1-77 or 1-78.
                                                                          NEW!


Potassium                                                  K

Reaction basis:
Potassium reacts with sodium tetraphenylborate to form an
insoluble compound which can be measured as turbidity.
NH4+, Hg+, Cs+ and Rb+ cause high results, because they
form a turbidity with the reagent as well. Up to 10 mg/l NH4+      Residual hardness see total Hardness                       °d
can be masked by adding 1 ml sodium hydroxide solution 5
% and 1 ml formaldehyde solution 5 % to 50 ml sample.
Strongly acidic, alkaline and buffered samples have to adjust-
ed to pH 10 – 11 for the test. Turbidities interfere and have to
be filtered prior to the test.
NANOCOLOR ® Potassium 50                      Cat. No. 985 045
Type:                    tube test 0-45
Measuring range:         2 – 50 mg/l K+
Shelf life:              at least 2 years
Sea water suitability:   yes, after dilution 1:10




www.mn-net.com                                                                                                               MN       87
                     The system for photometric water analysis

     Description of individual parameters and tests


     Silica / Silicon                                              SiO2               Sulphide / Hydrogen sulphide                              S2–

     Reaction basis:                                                                  Reaction basis:
     DIN method: in acidic solution dissolved silica and silicates                    DIN method: N,N-Dimethyl-1,4-phenylene diamine reacts
     react with ammonium molybdate to form yellow silicomolyb-                        with hydrogen sulphide to form a compound which changes
     dic acid. This is reduced to a blue compound by addition of a                    to leucomethylene blue. This compound is oxidised to
     reducing agent.                                                                   methylene blue using iron(III) ions.
     It is important that the distilled water used for filling up or dilu-            Sulphide is measured in an acidic medium; vigorous mixing
     tion is free of silica.                                                          can cause evaporation of gaseous hydrogen sulphide which
     NANOCOLOR ® Silica                                  Cat. No. 918 48              escapes analysis.

     Type:                     standard test 1-48                                     NANOCOLOR ® Sulphide                            Cat. No. 918 88
     Measuring range:          0.005 – 10.0 mg/l SiO2                                 Type:                    standard test 1-88
     Shelf life:               at least 3 years                                       Measuring range:         200 – 1000 mg/l SO42–
     Sea water suitability:    yes                                                    Shelf life:              at least 3 years
     Silica-free water                                Cat. No. 918 912                Sea water suitability:   yes
     for the analytical preparation, especially for minute concen-
     trations of silicon, and for dilution of water samples
     purity: < 0.005 mg/l SiO2
                                                                                      Sulphite                                                 SO3
     Sludge                                                                           Reaction bases:
     for sludge activity see TTC (page 73)                                            (a) Thiodibenzoic acid method: sulphite reacts with a deriv-
                                                                                      ative of thiodibenzoic acid to form a yellow colour complex,
     for sludge decomposition see page 103                                            which is evaluated photometrically. Contrary to the reduction
                                                                                      method (b), which also covers other reducing agents, this pro-
                                                                     CH2OH
                                                                                      cedure is selective for sulphite.
     Starch
                                                                             O

                                                                     OH
                                                                                  O


                                                                             OH       (b) Reduction method: sulphite ions bleach iodine solutions.
     Reaction basis:                                                                  The excess iodine is measured photometrically. Oxidising
     Starch reacts with iodine in sulfuric acid solution to form a                    and reducing substances interfere.
     blue inclusion complex.                                                          NANOCOLOR ® Sulphite 10                      Cat. No. 985 089
     Oxidising and reducing substances interfere.                                     Type:                  tube test 0-89
     NANOCOLOR ® Starch 100                           Cat. No. 985 085                Measuring range:       0.2 – 10.0 mg/l SO32–
                                                                                                             0.05 – 2.40 mg/l SO32–
     Type:                     tube test 0-85
                                                                                                             when using semi-micro cuvettes 50 mm
     Measuring range:          5 – 100 mg/l starch                                                           (Cat. No. 919 50)
     Shelf life:               at least 1 year                                        Reaction basis:        (a) thiodibenzoic acid method
     Sea water suitability:    yes, after dilution 1:1                                Shelf life:            at least 1 year
                                                                                      Sea water suitability: yes, after dilution 1:20
                                                                                      NANOCOLOR ® Sulphite 100                    Cat. No. 985 090
     Sulphate                                                      SO4                Type:                    tube test 0-90
                                                                                      Measuring range:         5 – 100 mg/l SO32–
     Reaction basis:
     turbidity measurement as barium sulphate                                         Reaction basis:          (b) reduction method
                                                                                      Shelf life:              at least 2 years
     Turbidities interfere and have to be filtered prior to the test.
     Good reproducibility is obtained for drinking, surface and                       Sea water suitability:   yes
     ground water. Polluted waste waters cause low results.
     NANOCOLOR ® Sulphate 200                         Cat. No. 985 086                Surfactants (detergents)
     Type:                     tube test 0-86                                         Detergents are surface-active substances, which are classi-
     Measuring range:          10 – 200 mg/l SO42–                                    fied into anionic, cationic and nonionic surfactants. They can
     Shelf life:               at least 3 years                                       be found in municipal and industrial waste waters.
     Sea water suitability:    no                                                     Reaction bases:
                                                                                      (a) Under suitable conditions anionic detergents react with
     NANOCOLOR ® Sulphate 1000                        Cat. No. 985 087                methylene blue to form a coloured complex, which is extract-
     Type:                     tube test 0-87                                         ed with an organic phase according to DIN 38 409-H23. Ref-
     Measuring range:          200 – 1000 mg/l SO42–                                  erence substance is methyl dodecylbenzene sulphonate
                                                                                      (MBAS: 342 g/mol).
     Shelf life:               at least 3 years
                                                                                      (b) Cationic detergents react with bromophenol blue to form
     Sea water suitability:    no                                                     a coloured complex, which is extracted with an organic
                                                                                      phase.

88     MN                                                                                                                             www.mn-net.com
                                       The system for photometric water analysis

Description of individual parameters and tests
Reference substance is N-cetyl-N,N,N-trimethylammonium
bromide (CTAB: 364.5 g/mol).
(c) Nonionic surfactants react with an indicator (TBPE =          Thiocyanate                                              SCN
Tetrabromphenolphthaleinethylester) to form a complex,
which is extracted with dichloromethane.                          Reaction basis:
                                                                  Thiocyanate reacts with iron(III) ions to form blood-red
If a water contains cationic and anionic detergents, equivalent
                                                                  iron(III) thiocyanate.
quantities are combined and escape analysis. Normally de-
tergents are determined as a sum. For the analysis of specific    The described method can also be used for detecting a thio-
detergents a correction factor has to be determined.              cyanate interference during cyanide determination (test 1-30
                                                                  and test 0-31).
NANOCOLOR ® Detergents anionic               Cat. No. 918 32
Type:                   standard test 1-32                        NANOCOLOR ® Thiocyanate 50                     Cat. No. 985 091
Measuring range:        0.02 – 5.0 mg/l MBAS                      Type:                    tube test 0-91
Reaction basis:         (a) methylene blue method                 Measuring range:         0.5 – 50.0 mg/l SCN–
Shelf life:             at least 3 years                          Shelf life:              at least 2 years
Sea water suitability: no                                         Sea water suitability:   yes, after dilution 1:1
The test kit contains chlorinated hydrocarbons; observe local
regulations.
NANOCOLOR ® Detergents cationic               Cat. No. 918 34
                                                                  Tin                                                       Sn
Type:                   standard test 1-34
Measuring range:        0.05 – 5.0 mg/l CTAB                      Reaction basis:
Reaction basis:         (b) bromphenol blue method                Photometric determination of dissolved tin(II) and tin(IV) with
                                                                  9-phenyl-3-fluorone.
Shelf life:             at least 3 years
Sea water suitability: no                                         Alkaline and buffered samples have to be adjusted to pH < 5
                                                                  with sulphuric acid prior to the test.
The test kit contains chlorinated hydrocarbons; observe local
regulations.                                                      NANOCOLOR ® Tin 3                              Cat. No. 985 097
NANOCOLOR ®      Nonionic surfactants 15 Cat. No. 985 047         Type:                    tube test 0-97
Type:                    tube test 0-47                           Measuring range:         0.10 – 3.00 mg/l Sn
Measuring range:         0.3 – 15.0 mg/l Triton® X-100            Shelf life:              at least 1 year
Reaction basis:          (c) TBPE method                          Sea water suitability:   yes, after dilution 1:10
Shelf life:              at least 2 years
Sea water suitability:   no


                                                                  TOC (Total organic carbon)                               TOC

                                                                  Reaction basis:
                                                                  clear and simple two-step procedure:
                                                                  1. removal of inorganic carbon (TIC)
                                                                  2. decomposition of organic carbon (TOC) and detemina-
                                                                      tion of the CO2 formed as colour decrease of an indica-
                                                                      tor.


                                                                  NANOCOLOR ® TOC 70                             Cat. No. 985 094
                                                                  Type:                    tube test 0-94
                                                                  Measuring range:         2 – 70 mg/l TOC
                                                                  Shelf life:              at least 1 year
                                                                  Sea water suitability:   no
                                                    NEW!




www.mn-net.com                                                                                                             MN       89
                    The system for photometric water analysis

     Description of individual parameters and tests


     TTC / Sludge activity                                       TPF      Zinc                                                        Zn

     Reaction basis:                                                      Reaction basis:
     Determination of the biochemical activity of sludge (activated       At pH 8.5 – 9.5 zinc ions react with zincon to form a blue col-
     sludge, digested sludge etc.) by means of the dehydrogenase          our complex.
     activity using 2,3,5-triphenyltetrazoliumchloride (TTC). Col-        Interfering metal ions are masked with cyanide. Acidic, alka-
     ourless TTC is converted into red triphenylformazane (TPF)           line and buffered samples have to be adjusted to pH 9 for the
     by dehydrogenases. The formed, water-insoluble TPF is dis-           test.
     solved in ethanol and is determined photometrically.
                                                                          For elimination of interfering calcium you may use the rea-
     This method allows the determination of the biochemical ac-          gents for lime precipitation.
     tivity of sludge samples, the characterisation of the action of
     waste waters and waste water constituents towards sludge             The concentration of chromium(III) must not exceed the zinc
     and a rapid visual evaluation of the degree of stabilisation of      concentration. Higher amounts of chromium(III) cause low re-
     a sludge.                                                            sults and have to be oxidised to chromium(VI) by boiling with
                                                                          ammonium peroxodisulphate / sulphuric acid.
     NANOCOLOR ® TTC / Sludge activity 150 Cat. No. 985 890               For higher calcium, iron and manganese concentrations ask
     Type:                   tube test 8-90                               for special instructions from MACHEREY-NAGEL.
     Measuring range:        5 – 150 µg TPF                               NANOCOLOR ® Zinc 4                            Cat. No. 985 096
     Shelf life:             at least 2 years at 2–8 °C
                                                                          Improved test kit!
     Sea water suitability: no
                                                                          Type:                    tube test 0-96
     For further information please see page 103
                                                                          Measuring range:         0.10 – 4.00 mg/l Zn2+
                                                                          Shelf life:              at least 1 year
                                                                          Sea water suitability:   yes, after dilution 1:1
                                                                          NANOCOLOR ® Zinc                               Cat. No. 918 95
                                                                          Type:                    standard test 1-95
     Turbidity                                                  FAU
                                                                          Measuring range:         0.02 – 3.00 mg/l Zn2+
     Reaction basis:                                                      Shelf life:              at least 3 years
     No reagents are required for this test. The turbidity of the pure    Sea water suitability:   yes
     sample is measured photometrically. NANOCOLOR® photo-
     meters are programmed with all necessary calibration date to         Reagents for lime precipitation               Cat. No. 918 939
     express turbidity as the spectral absorbance coefficient 1/m         Reagents for elimination of interfering calcium (up to 20 g/l
     (DIN EN 27 027) or in internationally valid Formazine Attenua-       Ca2+) for copper, nickel and zinc determinations.
     tion Units (FAU) which are based on formazine standard sus-
     pensions. NANOCOLOR® photometers measure the
     decrease of light intensity after passing the turbid sample. So-
     called turbidimeters measure the intensity of the light which is
     reflected by the undissolved particles in the sample. Thus, no
     factors are available for a conversion from FAU units (meas-
     ured with NANOCOLOR® photometers) to units which are
     shown by turbidimeters (NTU, FTU, FNU, Jackson Turbidity
     Units JTU or mg/l SiO2). However, comparison measurements
     often lead to reliable results. Blue colourations simulate turbid-
     ities.
     NANOCOLOR ® Turbidity                                  Test 1-92
     Type:                    no reagents required; test 1-92
     Measuring range:         1 – 100 FAU / 0.2 – 40.0 1/m
     Sea water suitability:   yes




90     MN                                                                                                                    www.mn-net.com
                               The system for the photometric water analysis

Special NANOCOLOR ® procedures · Overview

NANOCOLOR® digestions
Water samples are not always suited for direct analyses. Es-   Therefore, MACHEREY-NAGEL offers numerous digestion
pecially high contaminated waters and waste waters, sevage     systems for complex samples.
sludge and soil samples must be digested before the analysis   Heating blocks for sample digestions, see pages 70 and 71.
of certain parameters.
                                                                Page
Determination of total nitrogen TNb
Digestion with NanOx N in a heating block                          92
Pressurised microwave digestion with NanOx N                       93
Determination of total Phosphorus
Digestion with NanOx Metal in a heating block                      94
Pressurised microwave digestion with NanOx Metal                   94
Oxidation of samples containing heavy metals
Digestion with NanOx Metal in a heating block                      95
Pressurised microwave digestion with NanOx Metal                   95
Crack set with liquid reagents for the digestion of samples
containing heavy metals                                            96
Reagent set for oxidative digestion of sludge
containing heavy metals                                            96
Chemicals for special   NANOCOLOR®       procedures                97

Special procedures · sum parameters
AOX
Adsorbable organically bound halogens                              98
BOD5
Biochemical oxygen demand                                          99
COD
Chemical oxygen demand without toxic mercury salts                100
COD tube tests in accordance to ISO 15705                         101
Hydrocarbons / lipophilic substances                              102
TTC
Detection of microbial dehydrogenase activity                     103




www.mn-net.com                                                                                                     MN       91
                   The system for the photometric water analysis

     Special NANOCOLOR ® procedures · Determination of total nitrogen TNb
     In some countries government regulations require a complete                                        trogen-containing substances, a subsequent nitrate determi-
     nitrogen balance for effluents. Besides ammonium, nitrite and                                      nation gives the total nitrogen content in mg N per litre.
     nitrate, organic nitrogen compounds have to be determined –                                        In samples with low nitrate and nitrite concentrations this
     the sum of all these nitrogen compounds is called total nitro-                                     method directly gives similar results to the determination of
     gen.                                                                                               Total Kjeldahl Nitrogen TKN (including only ammonium and
     The Standard Method DIN EN ISO 11 905-1 describes the                                              organic nitrogen compounds). In the presence of detectable
     procedure for the determination of total N. After formation of                                     amounts of nitrate/nitrite, the TKN can be determined by sub-
     nitrate by oxidative digestion of all inorganic and organic ni-                                    tracting nitrate/nitrite nitrogen from total nitrogen.
     NanOx N · solid reagents for oxidative digestion of samples containing nitrogen compounds
     The NANOCOLOR® system offers two rapid and convenient                                              a) digestion in a heating block
     methods for sample preparation with solid NanOx N rea-                                                 easy handling
     gents:
                                                                                                            choice for everyday routine analyses
     a) regular digestion in a heating block
                                                                                                        For this digestion the following reagents and accessories are
     b) vigorous pressurised digestion in a microwave oven.                                             required:
     NanOx N consists of convenient solid oxidative digestion re-                                       NANOCOLOR ® total Nitrogen TNb 22                                Cat. No. 985 083
     agents (potassium peroxodisulphate) and solid compensa-
     tion reagents for elimination of interfering substances. After                                     Type:                                        tube test 0-83
     digestion with NanOx N, the samples can be analysed with                                           Measuring range:                             0.5 – 22.0 mg/l N
     NANOCOLOR® Nitrate tube or standard tests.                                                         Shelf life:                                  at least 1 year
                                                                                                        Sea water suitability:                       no
                                                                TNb                                     or
                                                                                                        NANOCOLOR ® total Nitrogen TNb 220                               Cat. No. 985 088
       organic         Ammonium-                                        Nitrite-       Nitrate-         Type:                    tube test 0-88
       nitrogen         nitrogen                                       nitrogen        nitrogen         Measuring range:         5 – 220 mg/l N
        org.-N           NH4-N                                          NO2-N           NO3-N           Shelf life:              at least 1 year
                                                               NanOx N                                  Sea water suitability: no
                                                                                                        convenient combinations of NanOx N reagents, predosed tube
       10 min in the microwave                                     or 0.5 – 1 h in the heating block    tests for determination of total N and digestion tubes (sufficient
                               photometric determination                                                for 20 tests).
                                      as nitrate                                                        or
                                                                                                        NANOCOLOR ® NanOx N solid reagents Cat. No. 918 979
                                                                                                        + empty reaction tubes 14 mm ID                 Cat. No. 916 80
                                                                                                        + NANOCOLOR ® Nitrate 50                       Cat. No. 985 064
                                                                                                        Type:                    tube test 0-64
                                                                                                        Measuring range:         0.5 – 22.0 mg/l N
                                                                                                        Shelf life:              at least 2 years
                                                                                                        Sea water suitability: no
                                                                                                        or
                                                                                                        NANOCOLOR ® NanOx N solid reagents Cat. No. 918 979
                                                                                                        + empty reaction tubes 14 mm ID                 Cat. No. 916 80
                                                                                                        + NANOCOLOR ® Nitrate                           Cat. No. 918 65
                                                                                                        Type:                    standard test 1-65
                                                                                                        Measuring range:         0.9 – 30.0 mg/l N
                                                                                                        Shelf life:              at least 2 years
                                                                                                        Sea water suitability: no
                                                                                                        Dilution of the sample can considerably increase the measur-
                                                                                                        ing ranges.
                                                                         digestion with NanOx N in a heating block

                                                                                                                                                                    0.2 – 0.5 ml
                                                          1x




                      NanOx N                                                                                    NanOx N
                                                                                                                Kompensationsreagenz
                      Aufschlussreagenz
                                                                                                                compensation reagent
                     decomposition reagent

                                                                                                                MACHEREY-NAGEL
                     MACHEREY-NAGEL
                                                                                                                 Postfach 10 13 52 · D-52313 Düren
                      Postfach 10 13 52 · D-52313 Düren




                   5 ml

     Mix sample with NanOx N                                          Boil: 30 minutes at 120 °C       Add compensation reagent.                           Decomposed sample for the
     digestion reagent.                                               or 1 hour at 100 °C.                                                                 test procedure.

92     MN                                                                                                                                                                 www.mn-net.com
                                                      The system for the photometric water analysis

Special NANOCOLOR ® procedures · Determination of total nitrogen TNb

b) Pressurised microwave digestion
   easy handling                                                                        For microwave digestion with NanOx N you need a micro-
   rapid procedure                                                                      wave oven featuring a time setting in seconds and a power of
                                                                                        minimum 750 W and maximum 900 W with the accessories
   high recovery rates                                                                  listed below.
   recommended when digestion in a heating block under nor-
   mal pressure has failed
                                                       Pressurised microwave digestion with NanOx N
                                                                                                                                                           0.2 – 0.5 ml
                                                       23 – 36 seconds            ~ 5 minutes
              2x

                                                                     0024


                                                                    900W


                 NanOx N
                                                                                                             NanOx N
                Aufschlussreagenz
               decomposition reagent                                                                        Kompensationsreagenz
                                                                                                            compensation reagent

               MACHEREY-NAGEL

                Postfach 10 13 52 · D-52313 Düren                                                           MACHEREY-NAGEL

                                                                                                             Postfach 10 13 52 · D-52313 Düren




     10 ml

Mix sample with NanOx N                             Microwave               Let cool in cold water.    Add compensation                               Decomposed
digestion reagent.                                  digestion.                                         reagent.                                       sample for the
                                                                                                                                                      test procedure.


Description                                                                                                                                      Pack of       Cat. No.
Accessories for microwave digestion
PTFE pressure vessel 20 ml for digestion, with safety expansion reservoir, set of sealing disks/safety dia-                                       1 kit         916 40
phragms
PTFE pressure vessel 20 ml                                                                                                                          1           916 43
PFA sealing disks                                                                                                                                  20           916 44
PTFE safety diaphragms                                                                                                                             10           916 45
PFA safety expansion reservoir                                                                                                                      1          916 40.1
PFA tubing with fitting                                                                                                                              1           916 46
PTFE boiling chips                                                                                                                                  3           916 28
Filter flocs MN 101                                                                                                                                500 g        481 100
Filter flocs MN 101                                                                                                                               1000 g        481 110




www.mn-net.com                                                                                                                                                     MN     93
                   The system for the photometric water analysis

     Special NANOCOLOR ® procedures · Determination of total phosphorus
     The element phosphorus occurs in many different circulations
     on earth. In waters phosphorus containing substances are
     unwanted, whereas in soil phosphorus is an important fertiliz-
     er for the growth of plants. Phosphates occur in many differ-
     ent waters, mainly as ortho- and poly-phosphates. Most
     phosphates reach the waters via the municipal and industrial
     waste water. They derive from detergents, residues of human
     and animal excrements and from soluble contents of fertiliz-
     ers. The discharge of phosphates to rivers and lakes leads to
     eutrophication, an increased growth of algae and water
     plants.
     In many countries governmental regulations require
     a complete phosphorus balance for effluents. Besides ortho-
     phosphates, also phosphonates and organic phosphorus
     compounds have to be determined – the sum of all these
     compounds is called total phosphorus. After formation of
     phosphate by oxidative digestion of all inorganic and organic
     phosphorus-containing substances, a subsequent determi-
     nation of phosphate gives the total phosphorus content in mg
     P per litre.                                                     b) NANOCOLOR ® standard tests and solid rea-
     The NANOCOLOR® system offers several rapid and conven-              gents NanOx Metal for digestion in a heating
     ient methods for sample preparation and determination of to-        block
     tal phosphorus.
                                                                      For frequent analyses of large numbers of samples, NANO-
     a) NANOCOLOR ® tube tests for digestion in a                     COLOR® standard tests are the choice for determination of
                                                                      total P. For a correct determination of total P the following re-
        heating block                                                 agents and accessories are required:
        solid reagent capsules for digestion                          NanOx Metal solid reagents                    Cat. No. 918 978
        predosed tube tests for determination of total P              + empty reaction tubes 14 mm ID                 Cat. No. 916 80
        digestion and photometric evaluation in one and the same      + NANOCOLOR ® ortho Phosphate                  Cat. No. 918 77
        test tube
                                                                      Type:                 standard test 1-77
        the choice for everyday routine analyses                      Measuring range:      0.04 – 6.5 mg/l P
     For a correct determination of total P with NANOCOLOR®           Shelf life:           at least 3 years
     tube tests, the following reagents are available:                or
     NANOCOLOR ® ortho- / total Phosphate 1 Cat. No. 985 076          NanOx Metal solid reagents               Cat. No. 918 978
                                                                      + empty reaction tubes 14 mm ID           Cat. No. 916 80
     Type:                  tube test 0-76
     Measuring range:       0.010 – 1.5 mg/l P                        + NANOCOLOR ® ortho Phosphate                  Cat. No. 918 78
     Shelf life:            at least 1 year                           Type:                    standard test 1-78
     NANOCOLOR ® ortho- / total Phosphate 5 Cat. No. 985 081          Measuring range:         0.2 – 17 mg/l P
                                                                      Shelf life:              at least 3 years
     Type:                  tube test 0-81
     Measuring range:       0.20 – 5.00 mg/l P
     Shelf life:            at least 1 year                           c) Pressurised microwave digestion
     NANOCOLOR ® ortho- / total Phosphate 15 Cat. No. 985 080            easy handling
     Type:                  tube test 0-80                               rapid procedure
     Measuring range:       0.30 – 15.00 mg/l P                          high recovery rates
     Shelf life:            at least 1 year
                                                                         recommended when digestion in a heating block under nor-
     NANOCOLOR ® ortho- / total Phosphate 45 Cat. No. 985 055            mal pressure has failed
     Type:                  tube test 0-55                            For microwave digestion of total phosphorus you need
     Measuring range:       5.0 – 50.0 mg/l P
     Shelf life:            at least 1 year                           NanOx Metal solid reagents                    Cat. No. 918 978
                                                                      a microwave oven featuring a time setting in seconds and a
     NANOCOLOR ® ortho- / total Phosphate 50 Cat. No. 985 079         power of minimum 750 W and maximum 900 W with the
     Type:                  tube test 0-79                            accessories listed on page 93 as well as the
     Measuring range:       10.0 – 50.0 mg/l P                        NANOCOLOR ® tube tests ortho- and total Phosphate
     Shelf life:            at least 3 years                          or
                                                                      NANOCOLOR ® standard tests ortho Phosphate




94     MN                                                                                                             www.mn-net.com
                                                                   The system for the photometric water analysis

Special NANOCOLOR ® procedures · Oxidation of samples containing heavy metals
In practice an analysis often recovers only the quantity of met-                                      complexes and adsorptive compounds or interfering organic
als which is present in the sample as dissolved ions. If the to-                                      substances are eliminated. Thus, maximum recovery rates
tal content of a metal has to be determined, particularly                                             can be achieved for the analysis of heavy metals.
samples of highly contaminated water or industrial waste wa-                                          The NANOCOLOR® system offers various rapid and conven-
ter must be decomposed to avoid falsely negative or falsely                                           ient methods for sample preparation with solid reagents for
low results. Usually, by applying acid and heat, undissolved                                          regular digestion and liquid reagents for vigorous digestion.
metal oxides are dissolved, metal ions are released from

NanOx metal · solid reagents for oxidative digestion of samples containing heavy metals
NanOx Metal consists of convenient solid oxidative digestion reagents (potassium peroxodisulphate) and solid neutralisation rea-
gents for pH adjustment prior to the subsequent determination of metals.
a) Digestion in a heating block                                                                       NanOx Metal                                                                                     Cat. No. 918 978
                                                                                                      + empty reaction tubes 14 mm ID                                                                  Cat. No. 916 80
   easy handling
   choice for everyday routine analysis

                                                                      digestion with NanOx Metal in a heating block

                                                            1x




                            NanOx                                                                                 NanOx
                                 Metall                                                                               Metall
                          Aufschlussreagenz
                                                                                                                Neutralisationsreagenz
                         decomposition reagent
                                                                                                                neutralisation reagent
                        MACHEREY-NAGEL
                                                                                                                MACHEREY-NAGEL
                          Postfach 10 13 52 D-52313 Düren
                                                                                                                 Postfach 10 13 52 D-52313 Düren




              5 ml

Mix sample with NanOx Metal                                         Boil: 30 minutes at 120 °C      Add neutralisation reagent.                                                            Decomposed sample for
digestion reagent.                                                  or 1 hour at 100 °C.                                                                                                   test procedure.

b) Pressured microwave digestion                                                                       After digestion with NanOx Metal, the samples can be
   easy handling                                                                                       analysed with the following NANOCOLOR® tests:
   rapid procedure                                                                                     Test 0-98 Aluminium 07 *                                                                   Test 1-53   Copper
   high recovery rates                                                                                 Test 1-02 Aluminium *                                                                      Test 0-37   Iron 3
   recommended when digestion in a heating block under                                                 Test 0-14 Cadmium 2                                                                        Test 1-36   Iron
   normal pressure has failed                                                                          Test1-13 Cadmium                                                                           Test 0-61   Nickel 7
For microwave digestion with NanOx Metal you need a micro-                                             Test 0-24 Chromate 5 (Chromium)                                                            Test 1-62   Nickel
wave oven featuring a time setting in seconds and a power of                                           Test 1-25 Chromate (Chromium)                                                              Test 1-95   Zinc
minimum 750 W and maximum 900 W with the accessories
listed on page 93.                                                                                     Test 0-54 Copper 7                                                                         Test 0-96   Zinc 4
                                                                                                       * only with microwave digestion

                                                                  Pressurised microwave digestion with NanOx Metal

                                                                    23 – 36 seconds              ~ 5 minutes
               2x

                                                                                  0024


                                                                                 900W

                  NanOx
                      Metall                                                                                                                              NanOx
                Aufschlussreagenz                                                                                                                             Metall
               decomposition reagent                                                                                                                    Neutralisationsreagenz
                                                                                                                                                        neutralisation reagent
                MACHEREY-NAGEL


                Postfach 10 13 52 D-52313 Düren                                                                                                         MACHEREY-NAGEL

                                                                                                                                                         Postfach 10 13 52 D-52313 Düren




      10 ml

Mix sample with                                                  Microwave                Let cool in cold water.                                  Add neutralisation                                 Decomposed
NanOx Metal                                                      digestion.                                                                        reagent.                                           sample for the
digestion reagent.                                                                                                                                                                                    test procedure.




www.mn-net.com                                                                                                                                                                                                     MN    95
                    The system for the photometric water analysis

     Special NANOCOLOR ® procedures · Oxidation of samples containing heavy metals

     NANOCOLOR ® Crack set
     Liquid reagents for oxidative digestion of samples containing heavy metals
     The crack set is used for oxidative sample preparation in              NANOCOLOR ® Crack set                          Cat. No. 918 08
     acidic medium (sulphuric acid / potassium peroxodisulphate)
     under normal pressure at 100 °C in a heating block. The crack          Reagent set for sample preparation
     set is recommended                                                     Shelf life: at least 3 years
         for complete and vigorous digestion of very resistant              Reaction basis:
         samples                                                            Digestion with sulphuric acid/potassium peroxodisulphate at
                                                                            100 – 120 °C. Some stable heavy metal cyanide complexes
        when microwave digestion with NanOx has failed                      are not completely destructible.
        when microwave digestion can not be applied because                 The NANOCOLOR® reagent set Crack set contains all rea-
        of high organic contamination of the sample                         gents required for the oxidative digestion and subsequent
     After digestion, the samples can be analysed in accordance             neutralisation.
     with the instructions for the respective NANOCOLOR® tests.             Additionally, a heating block with 22 mm borings and reagent
     For this digestion the following reagents and accessories are          sets for determination of the individual metals are required
     required:

      Description                                                                                                   Pack of       Cat. No.
      Accessories for crack set
      Tube for sample digestion 22 mm diameter, NS 19/26, with glass stopper                                           2          916 66
      Condenser 200 mm, type KS, NS 19/26 bottom and NS 29/32 top, with 3 m polyethylene tubing                        1          916 67
      Absorption attachment for condenser, NS 29/32                                                                    1          916 68

     NANOCOLOR ® reagent set Sludge 50
     Liquid reagents for oxidative digestion of sludge containing heavy metals
     In Germany, the use of sludge for soil improvement in farming          Reaction basis:
     and forestry and in gardening is regulated by government reg-          Mineralisation of sludge and soil samples with aqua regia at
     ulations which define the maximum contents of 7 heavy met-             100 °C and preparation of a solution for metal analysis ac-
     als (Cd, Cr, Cu, Hg, Ni, Pb, Zn) allowable in sludge for the           cording to DIN 38-414-S7. Additionally, reagent sets for de-
     above applications. The NANOCOLOR® reagent set sludge                  termination of the individual metals are required.
     50 allows even non-chemists to determine these contents
     with high accuracy. It is, however, recommended that you ar-            After digestion the sample can be analysed with the fol-
     range for the supplier of the kit to provide instructions to those      lowing NANOCOLOR® tests
     members of your team likely to be involved in its use.                  Test 1-132 Cadmium                     1 – 100 *
     With the exception of mercury and lead all of the above met-            Test 1-253 + 1-25 Chromium            20 – 1800 *
     als can be determined with the NANOCOLOR® analytical                    Test 1-53 Copper                      20 – 4000 *
     system. The photometers NANOCOLOR® 250 D and 350 D
     cannot be used for this sludge analysis. When using the pho-            Test 1-62 Nickel                       2 – 400 *
     tometers NANOCOLOR® 500 D, Linus, 400 D or 300 D, you                   Test 1-95 Zinc                        80 – 6000 *
     can directly read results as mg/kg dried residue of sludge (S).         * ranges in mg/kg for NANOCOLOR ® 500 D, 400 D, Linus
     For a digestion of sludge we recommend the following rea-               and 300 D
     gents and accessories:

     NANOCOLOR ® Sludge                            Cat. No. 918 50
     NANOCOLOR ® Sludge                           Cat. No. 918 501
     Reagent sets for digestion with aqua regia
     Shelf life:              at least 3 years
     Reagent set 918 501 does not contain the nitric acid 65 % and
     hydrochloric acid 37 % required for the digestion and is thus
     suited for airfreight. The acids have to be purchased sepa-
     rately from a local laboratory supply store.

      Accessories for sludge analysis                                                                                             Cat. No.
      Combination of all equipment required for sludge analysis (without reagents, photometer and heating block): 1 mor-           916 10
      tar with pestle, 2 tubes for sample digestion with stoppers, 1 condenser with connecting tubing, 1 absorption attach-
      ment, 100 filter circles MN 1670, 11 cm diameter, 100 filter circles MN 640 d, 15 cm diameter, 1 plastic wash bottle
      500 ml, 1 holder for round glass tubes and tubes for sample digestion, 2 volumetric flasks 100 ml, 1 Erlenmeyer
      flask 200 ml, 1 measuring cylinder 50 ml, 1 pipette 50 ml, 1 glass funnel each with 60 mm and 80 mm diameter, 100
      test sticks pH-Fix 0 – 14, 1 double spatula 180 mm, safety glasses, bulb for filling 20 ml pipettes, instructions for use


96     MN                                                                                                                  www.mn-net.com
                           The system for the photometric water analysis

Special NANOCOLOR ® procedures · Chemicals for special NANOCOLOR ® procedures
Distilled water                                                 Chloride complexing agent
                            For dilutions,                                                        Reagent for COD analysis
                            plastic bottle 1 l                                                    in samples with chloride
                            Cat. No. 918 932                                                      concentrations of 1000 –
                                                                                                  7000 mg/l
                                                                                                  Cat. No. 918 911




Silica-free water                                               Amidosulphuric acid
                            For the preparation of a                                              Reagent for the elimination
                            blank value for the determi-                                          of nitrite interferences
                            nation of silica,                                                     Cat. No. 918 973
                            plastic bottle 1 l
                            Cat. No. 918 912




COD-free water                                                  Reagents for sample preparation by clarification
                                                                precipitation
                                For dilutions of samples
                                prior to COD analysis.                                            Carrez solution 1 + 2, for
                                Distilled water and other di-                                     the determination of nitrite
                                lution waters can contain                                         in cooling lubricants, seep-
                                COD                                                               age waters from waste de-
                                                                                                  posits etc.
                                Cat. No. 918 993                                                  Cat. No. 918 937




Isobutyl methyl ketone (MIBK)                                   Reagents for lime precipitation
                                Extracting reagent for tube                                       Removal of interfering cal-
                                test phenolic index 5                                             cium for determinations of
                                Cat. No. 918 929                                                  copper, nickel and zinc
                                                                                                  Cat. No. 918 939




www.mn-net.com                                                                                                          MN       97
                   The system for the photometric water analysis

     Special NANOCOLOR ® procedures · AOX (adsorbable organically bound halogens)
     The AOX content represents the sum of organically bound        AOX analysis has never been so easy and reli-
     halogens (chlorine, bromine, iodine) which are adsorbable      able before!
     to a suitable adsorbent; their concentration is expressed as
     chloride.                                                        For a correct determination of AOX you need the following
     The rapid test NANOCOLOR® AOX 3 allows the determina-            reagents and accessories:
     tion of AOX in natural waters, industrial or municipal waste     NANOCOLOR ® AOX 3                         Cat. No. 985 007
     waters and sea water. The test is based on an easy three-           Type:                      tube test 0-07
     step procedure:                                                     Measuring range:           0.1 – 3.0 mg/l AOX
     1. Solid phase extraction with the NANOSORB ad-                                                0.01 – 0.30 mg/l AOX
         sorbent for AOX according to DIN 38 409-22                        Shelf life:              at least 1 year
     2. Oxidative digestion of the enriched adsorbent in a                 Sea water suitability:   yes, with 200 ml rinsing
         heating block at 120 °C or in a microwave oven                                             solution
     3. Photometric determination of AOX as chloride
     Benefits of NANOCOLOR ® AOX 3
        The complete test procedure takes less than 60
                                                                           NANOCOLOR ® starter set for AOX 3
        minutes – when using a microwave oven for the                                                    Cat. No. 916 111
        digestion, first results are obtained in even less
                                                                           Includes useful accessories for the determination of
        than 30 minutes.
                                                                           AOX with reagent set AOX 3; recommended to be or-
        No expensive instrumentation is required; the test                 dered with the first order of reagent set AOX 3
        can be performed and evaluated with the usual
        equipment for photometric water analysis.
        In contrast to carbon-based adsorbents, the use of                 Supplement kit for AOX 3            Cat. No. 918 072
        NANOSORB for AOX, a polymeric adsorbent pad,                       Contains additional oxidising reagents for the sen-
        avoids interferences by inorganic halides, which can               sitive AOX range (0.01 – 0.3 mg/l AOX) as well as for
        be removed entirely from the adsorbent. Even in                    COD-contaminated samples between 50 and 1000
        sea water, with chloride contents of up to 20 g/l (!),             mg/l COD
        reproducible results are obtained.                                 Shelf life:            at least 1 year
        For convenient handling, NANOSORB for AOX
                                                                           Pump set for AOX 3                  Cat. No. 916 115
        pads come individually prepackaged in plastic car-
        tridges, protecting the adsorbent during adsorption.               Required to increase the sensitivity of reagent set
        The use of predosed reagents shortens the pro-                     AOX 3 or simply a useful auxiliary for convenient
                                                                           solid phase extraction with NANOSORB for AOX
        cedure and reduces the analyst’s exposure to
        chemicals to a minimum.
        Outstanding recovery rates can be achieved,
        even in undiluted samples with high COD con-
        tents.
        Application of the automatic pump set increases the
        sensitivity of the test kit and facilitates the use of
        NANOSORB for AOX.




98     MN                                                                                                       www.mn-net.com
                              The system for the photometric water analysis

Special NANOCOLOR ® procedures · BOD5 (biochemical oxygen demand)
Together with the chemical oxygen demand (COD) the bio-         For a correct determination of the BOD5 you need the follow-
chemical oxygen demand (BOD) is the most important sum          ing reagents and accessories:
parameter for an estimation of the pollution of waste water.
The BOD is defined as the amount of oxygen per volume,
which is used by the microorganisms at 20 °C for oxidative      a) BOD5 determination in Winkler bottles
degradation of the organic substances present in the water.        according to DIN EN 1899-1-H51
Normally, the BOD is determined over a period of 5 days, and
is then called BOD5. MACHEREY-NAGEL offers two tests for        NANOCOLOR ® BOD5                              Cat. No. 985 822
the determination of the BOD5: a method with sample incuba-
tion in Winkler bottles, in accordance with DIN EN 1899-1-      Type:                    in Winkler bottles, according to
H51, and a rapid simplified tube test.                                                   DIN EN 1899-1-H51; test 8-22
Benefits of NANOCOLOR ® BOD5                                    Measuring range:         2 – 3000 mg/l O2
                                                                Shelf life:              at least 2 years
     recovery rates within the tolerances stated in DIN EN      Sea water suitability:   yes
     1899-1-H51
                                                                BOD5 accessories set                          Cat. No. 916 918
   oxygen measurement similar to DIN EN 25813-621, no
   manometers required                                          Includes useful accessories for sample preparation prior to
                                                                the determination of BOD5, recommended to be ordered with
   use of a nitrification inhibitor avoids oxygen consump-
                                                                the first order of the tube test BOD5.
   tion caused by oxidation of ammonium and nitrite (nitrifi-
   cation)                                                      The accessories set consists of an electric air pump, 2 aerat-
                                                                ing bricks, tubing, a 10-l PE container, a 1-l laboratory bottle
   sample incubation in Winkler flasks in accordance with       and 4 Winkler bottles.
   DIN EN 1899-1-H51 or
                                                                BOD5 nutrient mixture                         Cat. No. 918 994
   simplified incubation and oxygen measurement in one
                                                                Without nitrification inhibitor N-allylthiourea (ATU).
   and the same test tube
                                                                Ready-to-use supplementary reagents for preparation of the
   photometric evaluation with all types of NANOCOLOR®          inoculating water for BOD5 test 822.
   photometers – no additional instrumentation necessary
                                                                BOD5 nutrient mixture Plus                    Cat. No. 918 995
   automatic calculation of BOD5 with the photometer type
   NANOCOLOR® 500 D or                                          With nitrification inhibitor N-allylthiourea (ATU).

   clear evaluation form for hand-written step-by-step cal-     ready-to-use supplementary reagents for preparation of the
                                                                inoculating water for BOD5 test 822.
   culation of the BOD5
NEW! Simple determination of the biochemical oxygen de-
mand after 5 days (BOD5) of undiluted samples without using     b) BOD5 determination as simplified tube test
a control in accordance with DIN EN 1899-2-H52! The oxygen
enriched, undiluted sample is incubated in test tubes for 5     NANOCOLOR ® BOD5-TT                           Cat. No. 985 825
days at 20 ± 1°C in the dark. Determination of the dissolved
oxygen after 5 days is based on the Winkler procedure, DIN      Type:                     tube test 8-25
EN 25813-G21.                                                   Measuring range:          2 – 3000 mg/l O2
                                                                Shelf life:               at least 2 years
                                                                Sea water suitability: yes
                                                                Includes nitrification inhibitor N-allylthiourea (ATU) to prevent
                                                                falsely high BOD5 results due to high ammonium concentra-
                                                                tions.
                                                                BOD5-TT accessories set                       Cat. No. 916 925
                                                                Includes useful accessories for sample preparation prior to
                                                                the determination of BOD5-TT, recommended to be ordered
                                                                with the first order of the tube test BOD5-TT
                                                                The accessories set consists of an electric air pump, 2 aerat-
                                                                ing bricks, tubing, a 1000-ml PE container and two 40-ml re-
                                                                action vessels.


                                                                 Spare parts:                                         Cat. No.
                                                                 4 oxygen bottles according to Winkler                916 919
                                                                 4 aerating bricks                                    916 920
                                                                 10 reaction vessels for BOD5-TT                      916 926




www.mn-net.com                                                                                                              MN      99
                    The system for the photometric water analysis

      Special NANOCOLOR ® procedures · COD Hg-free
      The chemical oxygen demand (COD) is one of the most im-           Convenient chloride removal cartridges eliminate chloride in-
      portant parameters for the evaluation of industrial or munici-    terferences and the reliable dichromate chemistry leads to re-
      pal waste waters. It is defined as the amount of oxygen – in      producible and comparable results. The basic procedure of
      the form of an oxidizing agent – consumed in the oxidation of     the regular COD cuvette test has not been changed, so no
      organic water components. For standard methods (titration)        additional methods must be learned.
      and in cuvette tests, potassium dichromate in sulfuric acid
      has long been used as an oxidizing agent for COD analysis.
      In addition to organic substances, inorganic substances can       Procedure:
      be simultaneously oxidized.                                       1. Pretreatment in 1 minute
      Particularly higher chloride concentrations are known to inter-   2. Regular COD digestion (2 h at 148 °C in a heating block)
      fere. They may cause falsely higher results; but more often       3. Photometric measurement
      chloride interference leads to totally irreproducible results.
      Therefore, during COD analysis, chloride ions are usually         Benefits of the Hg-free NANOCOLOR® COD test:
      masked by mercury salts. However, mercury is toxic, may             Environment-friendly and analytically correct alternative
      cause damage to the nervous system of human beings and
                                                                          to regular COD tests
      animals and in addition is persistant in nature. So meanwhile
      in many countries the use of mercury compounds for analyti-         Established dichromate chemistry for results compara-
      cal purposes is subject to stringent regulations. Applications      ble to standard COD tests and conventional (Hg contain-
      of mercury-consuming analytical methods are forbidden as            ing) tube tests
      soon as they can be replaced by mercury-free methods.               No toxic compounds are used for chloride (salt) elimina-
      For the determination of the COD with tube tests one cannot         tion
      simply omit the mercury, since this may lead to false or irre-      For reproducible results, toxic mercury compounds are
      producible results, due to chloride/salt interferences. And us-     replaced by an effective non toxic salt removal technique
      ing a chemistry different to the potassium dichromate method        Use of chloride (salt) removing cartridges with capacity
      (e.g. permanganate or manganese) may cause conspicuous              of up to 2000 mg/l Cl– per cartridge
      difficulties in comparison to results obtained with standard        No vacuum pretreatment devices necessary for these
      methods or regular COD cuvette tests.                               cartridges
      Now, MACHEREY-NAGEL has developed a mercury-free
      COD test, which uses the established dichromate chem-
      istry, but substitutes the most toxic component – the
      mercury salts – by a non-toxic chloride elimination tech-
      nique using a special cartridge.




100     MN                                                                                                            www.mn-net.com
                                The system for the photometric water analysis

NANOCOLOR ® COD tube tests from MACHEREY-NAGEL in accordance to ISO 15705
The NANOCOLOR® system for photometric water analysis            NANOCOLOR® COD tube tests in accordance to ISO 15705
offers a complete system for the determination of the chemi-    NANOCOLOR ® COD 40                        Cat. No. 985 027
cal oxygen demand in accordance to the ISO norm 15705.
                                                                Type:            tube test 0-27
For the first time the ISO 15705 (“Water quality - Determina-
                                                                Measuring range: 2 – 40 mg/l O2
tion of the chemical oxygen demand index (ST-COD) - Small-
scale sealed tube method”) describes a photometric tube test
as a standardized and international accepted method for the     NANOCOLOR ® COD 60                        Cat. No. 985 022
analysis of water and waste water.                              Type:            tube test 0-22
MACHEREY-NAGEL confirms, that every component of the            Measuring range: 5 – 60 mg/l O2
NANOCOLOR® system for photometric water analysis fulfils
all requirements of the ISO 15705.                              NANOCOLOR ® COD 160                       Cat. No. 985 026
     sample volume of 2 ml                                      Type:            tube test 0-26
   predosed chemicals in round tubes                            Measuring range: 15 – 160 mg/l O2
   digestion conditions (148 °C / 2 h)
                                                                NANOCOLOR ® COD 1500                Cat. No. 985 029
   wavelength of photometric determination
                                                                Type:            tube test 0-29
                                                                Measuring range: 100 – 1500 mg/l O2
Benefits of NANOCOLOR® tube tests in comparison to
ISO 6060:
                                                                The photometric analysis is done
The new ISO 15705 is based on the same chemical reaction        with NANOCOLOR® photometers,
as the established ISO 6060. The water contents are oxidized    which can be operated without any
by sulphuric acid and potassium dichromate in the presence      keystroke, because all the informa-
of silver sulphate and mercury(II) sulphate. Compared with      tion is labelled on the bar-coded
the ISO 6060, the new method, described in the ISO 15705        NANOCOLOR® test tubes. Instant
has significant advantages:                                     cuvette detection by the built-in laser
    five times less mercury                                     scanner allows simple and fast rou-
   comparably less other toxic and hazardous reagents           tine analysis. The selection of the
                                                                right wavelength, depending on the
   all reagents are predosed in round tubes                     measuring range, and even the
   reduced risks for the user                                   measurements are done automati-
                                                                cally. The results are displayed di-
   reproducible results because of photometric determination
                                                                rectly in mg/l.




www.mn-net.com                                                                                                      MN       101
                    The system for the photometric water analysis

      Special NANOCOLOR ® procedures · Hydrocarbons / lipophilic substances
      The rapid test NANOCOLOR® HC 300 (Hydrocarbons) is              For a correct determination of hydrocarbons you need the fol-
      based on a MN-patented method. Hydrocarbons with a boil-        lowing reagents and accessories:
      ing temperature above 120 °C can be detected in a clear five-
      step procedure:                                                 NANOCOLOR ® HC 300                           Cat. No. 985 057
                                                                      after extraction from water or soil samples
      1. extraction of hydrocarbons from the sample
                                                                      Type:                   test tube 0-57
      2. removal of polar substances
                                                                      Range:                  0.5 – 5.6 mg/l HC
      3. evaporation of the extraction solvent                                                30 – 300 mg/kgHC
      4. oxidative digestion in a heating block                       Shelf life:             at least 1 year
      5. photometric determination of hydrocarbons                    Sea water suitability: yes
                                                                      Reaction tubes (empty, pack of 20)            Cat. No. 916 80
             Water sample                      Soil sample            Threaded couplings (pack of 2)                Cat. No. 916 04
       Extraction with pentane          Extraction with pentane       Stop valve s for piston pipettes (pack of 100)Cat. No. 916 21
       with reagent set 918 571         with reagent set 918 572
        in a separation funnel           in a Soxhlet apparatus       For determination of hydrocarbons in water
                                                                      Extraction kit HC from water               Cat. No. 918 571
                      removal of polar substances
                                                                      Shelf life:             at least 18 months
                      (CHROMABOND® ALOX N)
                              (if required)                           Separation funnel 500 ml (pack of 2)        Cat. No. 916 08
                                                                      Volumetric flask 25 ml (pack of 2)          Cat. No. 916 61
                      Evaporation of pentane in a
                                                                      For determination of hydrocarbons in soil
                            heating block
                                                                      Extraction kit HC from soil                Cat. No. 918 572
                     Photometric COD determina-                       Shelf life: at least 18 months
                          tion of the residue
                                                                      Soxhlet apparatus 30 ml                      Cat. No. 915 05
      Benefits of NANOCOLOR ®      HC 300                             (additionally a heater is required)
        applicable for water and soil                                 Extraction thimbles (pack of 25)           Cat. No. 645 008
        extraction without halogenated solvents                       Volumetric flask 50 ml (pack of 2)          Cat. No. 916 06
        mercurey-free reagents
                                                                      For removal of fatty acids, fat or other polar compounds
        outstanding selectivity
        complete removal of polar substances by solid phase           CHROMABOND® SPE columns ALOX N (pack of 20)
        extraction with CHROMABOND® ALOX N columns                                                        Cat. No. 730 250
        can be adapted to the determination of lipophilic sub-        Plastic syringes 50 ml (pack of 10)  Cat. No. 916 09
        stances                                                       Syringe adaptors (pack of 2)         Cat. No. 916 03
        evaluation with all types of NANOCOLOR® photometers
        – no expensive analytical instrumentation required




102     MN                                                                                                         www.mn-net.com
                                The system for the photometric water analysis

Special NANOCOLOR ® procedures · TTC (detection of microbial dehydrogenase activity)
The metabolic activities of microorganisms have a substantial     Benifits of NANOCOLOR® TTC / Sludge activity 150:
influence on the purification efficiency of a sewage plant.         The tube test contains all accessories necessary for the
Therefore it is important for the operator of a sewage plant to     procedure – no special equipment is required
know whether and in which way introduced waste waters af-
                                                                    The evaluation is possible with all NANOCOLOR® pho-
fect the metabolic activity of the microorganisms (the activat-
ed sludge activity).                                                tometers – no other instruments are necessary
                                                                    Procedure in accordance with German Standard Meth-
Microorganisms contain a number of enzymes (among others
                                                                    ods DEV-L3 and DEV-L4, but more convient handling
dehydrogenases), which are responsible for the degradation
of the waste water constituents. These enzymes are present          and faster results!
in all living microorganisms. The detection of dehydrogenas-        Rapid visual evaluation of the degree of stabilisation of a
es is therefore the proof for the occurrence of living biomass.     sludge is possible
The long-established tests for determination of the dehydro-
genase activity – standardized as German Standard Methods         NANOCOLOR ® TTC / Sludge activity 150          Cat. No. 985 890
(DEV) – are based on the so-called TTC method. Basis of this
procedure is the conversion of colourless 2,3,5-triphenyltetra-   Type:                    tube test 8-90
zolium chloride (TTC) to dark-red, water-insoluble 1,3,5-         Range:                   5 – 150 mg/l TPF
triphenylformazane (TPF), which is transferred to ethanol and                              0.050 – 2.300 E
determined either photometrically or visually via a rapid         Shelf life:              at least 2 years at 2 – 8 °C
screening test.                                                   Sea water suitability:   no



The tube test NANOCOLOR® TTC / Sludge activity 150 allows the application of the following methods:
   Determination of the biochemical activity (AS) of sludge samples in µg TPF/mg dry mass of sludge
   Characterisation of the action of waste water and waste water constituents towards sludge
   (stimulation or inhibition of the dehydrogenase activity DHA in %)
   Rapid visual evaluation of the degree of stabilisation of sludge based on a simple screening method




www.mn-net.com                                                                                                             MN       103
                     The system for the photometric water analysis

      NANOCOLOR ® accessories
      A comprehensive analytical system should include all acces-
      sories required for the analytical procedure, such as equip-
      ment for sampling, sample preparation and conservation, for
      digestion reactions, extraction and filtration – from analytical
      preparations to protective accessories for safe operation. All
      these components improve analytical procedures and thus
      help to obtain optimum results.
      NANOCOLOR ® membrane filters
      Removal of turbidities (fine filtration) is recommended for two
      reasons:
         Turbidities interfere with photometric determinations, in-
         tense turbidities must be removed prior to the analysis.
         When both soluble and total amounts of a substance
         (e.g. iron, manganese, COD etc.) have to be deter-
         mined, part of the sample has to be filtered to remove
         undissolved particles. The German Standard Methods
         use a pore size of 0.45 µm as standard for differenti-
         ation between dissolved and undissolved particles.
         Additionally we offer a pore size of 1.2 µm.




      Ordering informations
       Description                                                       Pack of      Cat. No.

       NANOCOLOR ®       membrane filters
       Membrane filtration kit 0.45 µm:                                    1 kit        916 50
       2 syringes 20 ml and 25 CHROMAFIL® membrane filters 0.45 µm
       CHROMAFIL® membrane filters 0.45 µm                                  50          916 52
       Membrane filtration kit 1.2 µm:                                     1 kit        916 511
       2 syringes 20 ml and 25 CHROMAFIL® membrane filters 1.2 µm
       CHROMAFIL® membrane filters 1.2 µm                                   50          916 513




      Piston pipettes and pipette stand
      Exact dosage of water sample and reagents is prerequisite
      for analytical accuracy. We offer a complete line of piston
      pipettes – with fixed or adjustable volume.
          convenient operation – exact dosage
         guarantees clean analytical work through use of dispos-
         able tips
         high security when working with toxic or corrosive sub-
         stances, because direct contact is avoided
         clean and safe storage of the valuable pipettes in a
         pipette stand
         all pipettes are supplied with tip ejector




104     MN                                                                        www.mn-net.com
                                The system for the photometric water analysis

NANOCOLOR ® accessories

Description                                                                                Pack of   Cat. No.
Piston pipettes and pipette stand
200 µl piston pipette (without tips)                                                         1       916 72
Plastic tips for 50 – 200 µl piston pipettes                                                100      916 915
500 µl piston pipette (without tips)                                                         1       916 53
Plastic tips for 200 – 1000 µl piston pipettes                                              100      916 76
1.0 ml piston pipette (without tips)                                                         1       916 71
Plastic tips for 200 – 1000 µl piston pipettes                                              100      916 76
2.0 ml piston pipette (without tips)                                                         1       916 917
Plastic tips for 1.0 – 5.0 ml piston pipettes (inner seal)                                  100      916 916
5 – 50 µl digital piston pipette, adjustable                                                 1       916 58
50 – 200 µl digital piston pipette, adjustable                                               1       916 914
Plastic tips for 5 – 50 and 50 – 200 µl piston pipettes                                     100      916 915
200 – 1000 µl digital piston pipette, adjustable                                             1       916 77
Plastic tips for 200 – 1000 µl piston pipettes                                              100      916 76
1.0 – 5.0 ml digital piston pipette, adjustable                                              1       916 909
Plastic tips for 1.0 – 5.0 ml piston pipettes (inner seal)                                  100      916 916
Pipette stand for 6 piston pipettes                                                          1       916 79

General analytical accessories
Reaction tubes 14 mm ID                                                                      20      916 80
Volumetric flask 10 ml for reduced analytical preparations                                     2      916 42
Volumetric flask 25 ml with NS 10/19 and polyethylene stopper for analytical preparations      2      916 61
Volumetric flask 100 ml with NS 12/21 and polyethylene stopper                                 2      916 83
Measuring cylinder 50 ml                                                                      1      916 84
Pipette 20 ml, short version                                                                  1      916 62
Bulb for filling pipettes                                                                      1      916 65




www.mn-net.com                                                                                           MN     105
                     Quality control for photometric water analysis

      NANOCOLOR ® accessories

       Description                                                                                                                  Pack of            Cat. No.
       General analytical accessories (cont.)
       Glass funnel 60 mm diameter                                                                                                     1               916 81
       Glass funnel 80 mm diameter                                                                                                     1               916 82
       Filter circles MN 1670, 11 cm diameter                                                                                        100               470 011
       Filter circles MN 640 d, 15 cm diameter                                                                                       100               205 015
       Plastic wash bottle 500 ml                                                                                                      1               916 89
       Magnetic stirring unit without heater                                                                                           1               970 115
       Mini-magnets for stirring                                                                                                       1               916 211
       Timer with digital display and acoustic signal (up to 99:59 min)                                                                1               916 96
       Electric air pump with delivery tube                                                                                            1               916 55
       Double spatula, 18/8 steel, 180 mm long                                                                                         1               916 94
       Porcelain mortar 90 mm diameter with pestle                                                                                     1               916 88
       Holder for 15 round glass tubes and 2 tubes for sample digestion                                                                1               916 36
       Safety bottle for shaking COD test tubes                                                                                        1               916 37
       Safety shield for heating block VARIO-1, transparent, for use with tube tests                                                 1 set             916 90




      The NANOCONTROL system for analytical quality control
      Reagents, measuring equipment and personal handling are              Composition and concentrations of single and multi stand-
      already subject to validation, or frequent internal control in       ards meet the requirements of specific user groups e. g. in
      many labs.                                                           waste water treatment, drinking water production and others.
      The NANOCONTROL system offers a complete range of                    The standards are supplied with a documentation form, in
      products for analytical quality control of analyses carried out      which expected concentration and given tolerance range
      with the NANOCOLOR® system.                                          (from/to) are stated for each parameter. In addition there is
                                                                           plenty of space for documentation of quality control. Applica-
          NANOCONTROL standards                                            tion of NANOCONTROL standards permit easy analytical
         NANOCONTROL 100+ addition (spike solution)                        quality control and simplify the corresponding documentation.
         NANOCONTROL NANOCHECK                                             Instead of the water sample, the standard solution is used.
                                                                           The consistent use of NANOCONTROL standards leads to
      NANOCONTROL standards allow the user to check all vari-              an objective proof of the accuracy of the analysis carried out
      ous parts of the analytical system:                                  with the NANOCOLOR® system and confirms the operator’s
         reagents                                                          confidence in the reliability of the measured results.
         measuring equipment, especially the photometer and
         heating block                                                                              NANOCONTROL 100+
         personal handling                                                                       standard addition (increase)
                                                                                                e.g. with Test 0-68 Nitrite 2
                                                                                   (increasing per 0.5 ml 100+ solution 0.02 mg/l NO2-N)
      NANOCONTROL standards                                                  a = sample            a = sample            a = sample             a = sample
                                                                             b = 100+ solution     b = 100+ solution     b = 100+ solution      b = 100+ solution




                                                                                                                                b   ← 1.0 ml           b   ← 1.5 ml
                                                                                                         b    ← 0.5 ml

                                                                              a                      a                      a                      a



                                                                             sample                sample + 1 x 100+     sample + 2 x 100+      sample + 3 x 100+

                                                                                  0.12                   0.14                   0.16                   0.18
                                                                             This example does not show any significant deviation in the sample.

      NANOCONTROL standards are ready-to-use standard solu-
      tions, which contain one or several substances in known con-
      centrations.



106     MN                                                                                                                                     www.mn-net.com
                                Quality control for photometric water analysis

The NANOCONTROL system for analytical quality control

NANOCONTROL single standards for analytical quality control
 NANOCOLOR ® test                Dimension                  Concentration        Addition per 0.5 ml               Number of      Cat. No.
                                                            of the standard        100+ spiking                      tests
                                                                solution               solution
 AOX 3 (test 0-07)               mg/l AOX                          1.0                   1.0                          20           925 07
 BOD5 (test 8-22 and 8-25)       mg/l O2                          210                     –                           10           925 82
 Chlorine (test 1-16)            mg/l Cl2                         1.00                    –                           30           925 17
 Chlorine 2 (test 0-17)          mg/l Cl2                         0.80                    –                           30
 Chromate 5 (test 0-24)          mg/l CrO42–                       2.0                   0.4                          15           925 24
 Chromate (test 1-25)            mg/l CrO42–                      0.40                   0.4                          15
 COD 60 (test 0-22, 0-27)        mg/l O2                           30                     –                           15           925 22
 COD 160 (test 0-26, 0-33,       mg/l O2                          100                     –                           15           925 26
                  0-94)          mg/l TOC                          40                     –                           15
 COD 15000 (test 0-23, 0-28)     g/l O2                            4.0                    –                        30 – 150        925 28
 COD 1500 (test 0-29)            mg/l O2                          400                     –                           15           925 29
 Nitrite (Test 1-67)             mg/l NO2-N / NO2–           0.060 / 0.20           0.02 / 0.065                      15           925 68
 Nitrite 2 (Test 0-68)           mg/l NO2-N / NO2–            0.30 / 1.00           0.02 / 0.065                      15
 ortho-Phosphate 1               mg/l PO4-P / PO43–           1.00 / 3.07            0.10 / 0.31                      15           925 76
 (Test 0-76, 1-77)
 Sulphite 100 (test 0-90)        mg/l SO32–                       50                        –                         15           925 90
 TOC see COD 160
 Note:                        For chlorine (tests 1-16 and 0-17) and sulphite (test 0-90), as standards simulation substances are
                              used, which react in the same way as the original parameter.




NANOCONTROL 100+ addition (spike solution)
Spike solutions (NANOCONTROL 100+ solution) allow trac-                                  NANOCONTROL 100+
ing of interferences, which may be present in the sample. Us-                     standard addition for multistandards
ing spike solutions is recommended whenever:
    you start a new analytical series with an unknown sam-
    ple matrix
    you know that your sample contains interfering sub-
    stances, e. g. proteins, larger amounts of salts etc.
    you find continuous major deviations from other analyti-
                                                                                                                                1 ml
    cal procedures
    you question the accuracy of your own analytical results
                                                                                                   100+ Addition
The principle of standard addition is, to increase the concen-
tration of a sample in defined steps. The recovery rate is then
an indication of possible interferences. When constant, pro-
portional deviations occur, they can be counterbalanced;                 100 ml                 pipette 1.0 ml         add 1 x 1.0 ml
thus, the result can be corrected.                                       sample solution        addition solution      100+ spiking
                                                                         to the ring mark                              solution
                                                                         e. g.
                                                                         result before          theoretical            result after
                                                                         addition               increase               standard addition
                                                                              0.73 mg/l            0.25 mg/l               0.98 mg/l




www.mn-net.com                                                                                                                          MN   107
                   Quality control for photometric water analysis

      The NANOCONTROL system for analytical quality control

      NANOCONTROL multistandards for analytical quality control
       Parameter     NANOCOLOR ® test                       Concentration of the          Confidence interval      Number of Cat. No.
                                                            standard solution                                       tests
      NANOCONTROL multistandard Sewage outflow incl. NANOCONTROL 100+ standard addition                            12 – 120                        925 011
      Ammonium       0-03     Ammonium 3                      0.75   mg/l   NH4-N        0.67   – 0.83     mg/l
                     0-04     Ammonium 10                     3.0    mg/l   NH4-N        2.7    – 3.3      mg/l
      COD            0-26     COD 160                       114      mg/l   COD        103      – 125      mg/l
                     0-33     COD 300                       114      mg/l   COD        103      – 125      mg/l
                                                                                                                           Multistandard
      Nitrate        0-64     Nitrate 50                      6.0    mg/l   NO3-N        5.2    – 6.8      mg/l           KA-Ablauf
                                                                                                                        Sewage, Outflow
      total N        0-83     total Nitrogen 22              12.0    mg/l   N           10.0    – 14.0     mg/l
      total P        0-76     Phosphate 1                     0.63   mg/l   P            0.56   – 0.70     mg/l
                     0-81     Phosphate 5                     2.50   mg/l   P            2.25   – 2.75     mg/l

      NANOCONTROL multistandard Sewage inflow incl. NANOCONTROL 100+ standard addition                             30 – 300                        925 012
      Ammonium       0-05     Ammonium 50                    25      mg/l   NH4-N       22      – 28       mg/l
      COD            0-29     COD 1500                      400      mg/l   COD        360      – 440      mg/l
      Nitrate        0-64     Nitrate 50                     15.0    mg/l   NO3-N       13.5    – 16.5     mg/l
                     0-66     Nitrate 250                    15      mg/l   NO3-N       13      – 17       mg/l            Multistandard

      total N        0-88     total Nitrogen 220             75      mg/l   N           67      – 83       mg/l           KA-Zulauf
                                                                                                                        Sewage, Inflow
      total P        0-80     Phosphate 15                    8.00   mg/l   P            7.20   – 8.80     mg/l
                     0-81     Phosphate 5                     3.20   mg/l   P            2.90   – 3.50     mg/l

      NANOCONTROL multistandard Seepage water incl. NANOCONTROL 100+ standard addition                            15 – 300                        925 013

      Ammonium       0-06     Ammonium 200                  80       mg/l   NH4-N      72       – 88       mg/l
      COD            0-23     COD 10000                      4.00    g/l    COD         3.60    – 4.40     g/l
                     0-28     COD 15000                      4.0     g/l    COD         3.6     – 4.4      g/l             Multistandard

      Nitrate        0-66     Nitrate 250                   30       mg/l   NO3-N      27       – 33       mg/l            Sickerwasser
                                                                                                                        Seepage water / Eaux d’infiltration

      total-P        0-55     Phosphate 45                  25       mg/l   PO4-P      22       – 28       mg/l
      ortho-P        0-79     ortho-Phosphate 50            25       mg/l   PO4-P      22       – 28       mg/l

      NANOCONTROL multistandard Metals 1 incl. NANOCONTROL 100+ standard addition                                  15 – 60                        925 015
      Cadmium        1-13     Cadmium                      0.10      mg/l   Cd2+        0.08    – 0.12     mg/l
                     0-14     Cadmium 2                    1.00      mg/l   Cd2+        0.80    – 1.20     mg/l
      Chloride       0-19     Chloride 200                80         mg/l   Cl–        70       – 90       mg/l
                     0-21     Chloride 50                 20         mg/l   Cl–        17       – 23       mg/l
      Chromium       0-243    total Chromium + Chromate 5 1.0        mg/l   Cr          0.8     – 1.2      mg/l
                     1-253    total-Chromium + Chromate 1.0          mg/l   Cr          0.8     – 1.2      mg/l
      Fluoride       0-40     Fluoride 2                   1.0       mg/l   F–          0.8     – 1.2      mg/l            Multistandard

                     1-42     Fluoride                     1.00      mg/l   F–          0.80    – 1.20     mg/l               Metalle 1
                                                                                                                              Metals 1
      Iron           1-36     Iron                         0.10      mg/l   Fe3+        0.08    – 0.12     mg/l
                     0-37     Iron 3                       1.0       mg/l   Fe3+        0.80    – 1.20     mg/l
      Sulphate       0-86     Sulphate 200                80         mg/l   SO42–      70       – 90       mg/l
      Zinc           1-95     Zinc                         0.10      mg/l   Zn2+        0.08    – 0.12     mg/l
                     0-96     Zinc 4                       1.0       mg/l   Zn2+        0.8     – 1.2      mg/l

      NANOCONTROL multistandard Metals 2 incl. NANOCONTROL 100+ standard addition                                     15                          925 016
      Copper         1-53     Copper                         0.60    mg/l   Cu2+        0.50    – 0.70     mg/l
                     0-54     Copper 7                       2.00    mg/l   Cu2+        1.80    – 2.20     mg/l
      Lead           0-09     Lead 5                         2.50    mg/l   Pb2+        2.25    – 2.75     mg/l
                     1-10     Lead                           0.25    mg/l   Pb2+        0.22    – 0.28     mg/l            Multistandard

      Nickel         0-61     Nickel 7                       2.00    mg/l   Ni2+        1.80    – 2.20     mg/l               Metalle 2
                                                                                                                              Metals 2

                     1-62     Nickel                         0.60    mg/l   Ni2+        0.50    – 0.70     mg/l
      Potassium      0-45     Potassium 50                  20       mg/l   K+         18       – 22       mg/l

      Multistandards can also be used for checking reagents and photometers from other manufacturers. The contents of one package
      of each multistandard are sufficient for at least 1 repeated test of each parameter indicated. If you only check one or a few pa-
      rameters, then the number of individual determinations is increased. Shelf life: 1 year, 6 weeks after opened

108    MN                                                                                                              www.mn-net.com
                                 Quality control for photometric water analysis

The NANOCONTROL system for analytical quality control

 Parameter       NANOCOLOR ® test                      Concentration of the         Confidence interval      Number of Cat. No.
                                                       standard solution                                      tests
NANOCONTROL multistandard Drinking water incl. NANOCONTROL 100+ standard addition                            15 – 30    925 018
Aluminium       1-02    Aluminium                        0.50 mg/l Al3+             0.45 – 0.55 mg/l
                0-98    Aluminium 07                     0.50 mg/l Al3+             0.44 – 0.56 mg/l
Ammonium        1-05    Ammonium                         0.20 mg/l NH4-N            0.17 – 0.23 mg/l
Chloride        1-20    Chloride                        20      mg/l Cl–           18       – 23       mg/l
                0-21    Chloride 50                     20      mg/l Cl–           18       – 23       mg/l        Multistandard
Iron            1-36    Iron                             1.50 mg/l Fe3+             1.35 – 1.65 mg/l               Trinkwasser

                0-37    Iron 3                           1.50 mg/l Fe     3+        1.30 – 1.70 mg/l
                                                                                                                   Drinking water


Manganese       1-60    Manganese                        1.50 mg/l Mn2+             1.35 – 1.65 mg/l
                0-58    Manganese 10                     1.5    mg/l Mn2+           1.3     – 1.7      mg/l
Sulphate        0-86    Sulphate 200                    80      mg/l SO42–         70       – 90       mg/l
Multistandards can also be used for checking reagents and photometers from other manufacturers. The contents of one package
of each multistandard are sufficient for at least 1 repeated test of each parameter indicated. If you only check one or a few pa-
rameters, then the number of individual determinations is increased. Shelf life: 1 year, 6 weeks after opened




NANOCONTROL NANOCHECK
Test solutions for the determination of photometric accuracy
Benefits: NANOCONTROL NANOCHECK is a secondary
standard for the control of inspection, measuring and test
equipment in accordance to ISO 9001 and ISO 14001. The
test solutions have been checked in a reference photometer
monitored with primary standards (NIST-Standards). The re-
sults are documented.
With only 2 stable colour solutions the wavelength accuracy
and the linearity of the absorbance measurement can be
checked.
                                                                  NEW!
NANOCONTROL NANOCHECK fullfils the requirements of
analytical quality control mentioned in the DWA-advisory leaf-
let A 704 (Operational Methods for the Self-Monitoring of
Wastewater Systems).



NANOCONTROL NANOCHECK                       Cat. No. 925 701
Shelf life:             1 year




www.mn-net.com                                                                                                             MN       109
                    Quality control for photometric water analysis


      Customer information
      Product literature                                                MACHEREY-NAGEL on the web
      A large number of catalogues, leaflets with new products,         MACHEREY-NAGEL presents its own homepage with versa-
      special instructions, supplements to photometer manuals and       tile product information from all product ranges. The informa-
      others are available for MACHEREY-NAGEL customers – of            tion is available in English or German.
      course free of charge.
      We also present our products at all major exhibitions for water
      analysis and are happy to meet you there.




                                                                        http://www.mn-net.com


                                                                        Sales and services
                                                                        All around the world, wherever you are, there is always an MN
                                                                        distributor nearby. Their staff can be relied on to give you al-
                                                                        ways the best possible advice about our products for water
                                                                        analysis. They assist with orders and provide you with all nec-
                                                                        essary service.
                                                                        If you request information about your local MN dealer, please
                                                                        do not hesitate to contact us.
                                                                        Phone: +49-2421-969-0
                                                                        Fax:        +49-2421-969-199
                                                                        e-mail:     sales@mn-net.com




      Quality assurance
      During production, filling and storage, NANOCOLOR® rea-
      gents are constantly controlled by a quality system which ful-
      fils the requirements of DIN EN ISO 9001:2000. Lot specific
      routine QA reports provide the constant quality of the calibra-
      tions of our NANOCOLOR® reagents and ensure accuracy of
      given results over the entire shelf life of NANOCOLOR® test
      kits.




110     MN                                                                                                             www.mn-net.com

				
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