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Genetic Approach towards Modulating

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Genetic Approach towards Modulating Powered By Docstoc
					  A genetic approach towards
  modulating unsynchronised
  pod shatter in Oilseed Rape
  (Brassica napus)




Thomas Wood, Østergaard Lab, Crop Genetics, JIC
     Pod shatter in Brassica napus

• Ave. 10% loss (>75%)
• Volunteer contamination

• Swathing, Desiccants
• Lack of resistant varieties
                POSH 1-3
DH Mapping Population
Segregates for Pod Shatter Resistance (PSR)
Multiple Determinants

Aim:
- Improvement of Linkage Map
- QTL Identification
- Differences in Pod Physiology
 Investigating factors regulating PSR
Physical Analysis:
- Randomised Impact Test
- Sectioning/Microscopy

Mapping:
- 200 loci genotyped
- Candidate Markers
- 2 QTL associated with
   PSR identified

                           POSH Marker Analysis (KW)
                 Thanks !
Acknowledgments:
• Østergaard Lab
• Bancroft Group
• Judith Irwin
• Dean Group
• Peter Werner, KWS
Progress curve for 20 pods samples, Susceptible & Resistant POSH lines,
                               0 - 1040s
                      20
                                                             Resistant
                                                             Susceptible


                      15
 No. of Intact Pods




                      10




                      5




                      0
                           0   200   400         600   800         1000
                                           Time, S

				
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posted:5/5/2013
language:English
pages:6