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					                    OIE Reference Laboratory Reports
                                           Activities in 2011


   Name of disease (or topic) for                                  Foot and mouth disease
 which you are a designated OIE
         Reference Laboratory:

                             Address of laboratory:     Regional Reference Laboratory for FMF in South East Asia
                                                                   National Institute of Animal Health
                                                                 Department of Livestock Development
                                                                  Pakchong, Nakhonratchasima 30130
                                                                              THAILAND


                                                Tel.:                +66 44 279112, +66 44 313869

                                                Fax:                 +66 44 314889, +66 44 313869


                                     e-mail address:                         rrl@dld.go.th,

                                            website:                       www. dld.go.th/rrl


      Name (including Title and                              Dr Wilai Linchongsubongkoch
Position) of Head of Laboratory                         Director, Regional Reference laboratory
          (Responsible Official):                           for FMD since 1st January to 30
                                                                    September 2011

                                                                Dr Panithan Thongtha
                                                         Acting Director, Regional Reference
                                                        Laboratory for FMD in South East Asia
                                                         Since 1st October 2011to the present

          Name(including Title and                         Dr Wilai Linchongsubongkoch
         Position) of OIE Reference                       Veterinary Expert, Consultant of
                            Expert:                     Department of Livestock Development

       Name (including Title and                            Dr Wilai Linchongsubongkoch
 Position) of writer of this report                          Dr Kingkarn Boosuya Seeyo
        (if different from above):                      Veterinary officer, Regional Reference
                                                        Laboratory for FMD in South East Asia




Annual reports of OIE Reference Centres, 2011                                                                      1
                                                Foot and mouth disease




                Part I: Summary of general activities related to the disease


    1. Test(s) in use/or available for the specified disease/topic at your laboratory


                   Test                          For                         Specificity                    Total

          Liquid phase blocking         Antibody to structural             Sera from cattle,                12,246
           ELISA (LP ELISA)              protein of FMDV                 buffaloes, pigs, goat,

         Indirect blocking ELISA      Antibody to non-structural           Sera from cattle,                9,199
                                            protein (NSP)                   buffaloes, pigs

          Antigen capture ELISA                Antigen                     Infected tissue from              834
                                                                         tongue epithelium and
                                                                           foot and laboratory
                                                                                 samples

           BHK-21 cell culture              Virus isolation              Filed isolate samples               398

                 RT-PCR                        Antigen               Tissue samples and virus                354
                                                                             isolation

                Sequencing                Molecular analysis              Field isolate viruses              107

           r-value by LP ELISA            Vaccine matching                Field isolate viruses               56



    2. Production and distribution of diagnostic reagents

      The diagnostic reagents produced by Regional Reference Laboratory for FMD in South East Asia (RRLSEA),
      Pakchong, Thailand was mainly ELISA reagents use in ELISA technique, the number of reagents produced in the
      year 2011 was shown in the table below:

      Table 1 Quantity of FMD diagnostic reagents that has been produced at RRLFMD in year 2011


            Item                              Description                                            Quantity (ml)

            1         Rabbit trapping antibody to FMDV
                      type O,                                                                  40
                      type A                                                                   98
                      type Asia1                                                               18

            2         Guinea pig detecting antibody to FMDV
                      type O,                                                                  112
                      type A                                                                   205
                      type Asia1                                                               81

            3         Inactivated FMD antigen (concentrated and partial purified)
                      type O,                                                                  200
                      type A                                                                   240
                      type Asia1                                                               200

            4         Strong positive control serum to FMDV type O, A and Asia1                240

            5         Weak positive control serum to FMDV type O, A and Asia1                  240




2                                                                              Annual reports of OIE Reference Centres, 2011
                                                    Foot and mouth disease




      Table 2. Number of FMDV diagnostic reagents and reference materials from RRLSEA supplied at the national
      and regional level for OIE member countries in 2011.

     Type of reagents        Supplied nationally       Supplied OIE           Total                     Remarks
                             and own lab               Members Countries

     ELISA reagent for       Type O = 23 sets          Type O = 9 sets        Type O = 32 sets          1 set can be
     FMDV Rabbit             Type A = 33 sets          Type A = 9 sets        Type A = 42 sets          tested for
     trapping antibody for   Type Asia1 = 23 sets      Type Asia1 = 9 sets    Type Asia1 = 32 sets      1000 samples
     type O, A, and Asia1

     Guinea pig detecting    Type O = 26 sets          Type O = 9 sets        Type O = 35 sets          1 set can be
     antibody for type O,    Type A = 36 sets          Type A = 9 sets        Type A = 45 sets          tested for
     A and Asia1             Type Asia1 = 30sets       Type Asia1 = 9 sets    Type Asia1 = 39 sets      1000 samples

     Concentrated            Type O = 165 ml           Type O = 9 ml          Type O = 174 ml
     inactivated (20X)       Type A = 210 ml           Type A = 9 ml          Type A = 219 ml
     antigen type O, A,      Type Asia1 = 180 ml       Type Asia1 = 9 ml      Type Asia1 = 189 ml
     Asia1

     Control serum for       C++ = 660 ml              C++ = 9 ml             C++ = 669 ml
     C++, C+ and C-          C+ = 510 ml               C+ = 9 ml              C+ = 519 ml
                             C- = 495 ml               C- = 9 ml              C- = 504 ml



                     Part II: Activities specifically related to the mandate
                                 of OIE Reference Laboratories

3.    International harmonisation and standardisation of methods for diagnostic testing or the

      The standardisation of test method for FMD diagnosis using antigen capture ELISA, LP ELISA, NSP test and
      virus isolation test have been harmonized since the first lab network meeting in 2005 and the standard operation
      procedure (SOP) of those test methods have been distributed to all member countries to use in their routine assays.

      a)    Establishment and maintenance of a network with other OIE Reference Laboratories
            designated for the same pathogen or disease and organisation of regular inter-laboratory
            proficiency testing to ensure comparability of results

            SEACFMD Laboratories network meeting has been organized annually under the support by OIR-SRR and
            OIE-RCU, the RRLSEA acts as the leader and co-organizer of this network. The network activities in the
            year 2011 as this follow:

                  The 6th meeting of SEACFMD laboratory network meeting and joint EpiNet meeting was held in
                   Pakchong, Thailand during 2-3 March 2011. Head of FMD national laboratories and Head of
                   epidemiologists from Cambodia, Brunei, China, Lao PDR, Malaysia, Myanmar, Philippines,
                   Indonesia, Vietnam, Thailand including the Representative from FAO, OIE-SRR were participated.
                   There was a special lecture on transportation of infectious substances by Dr Wilai
                   Linchongsubongkoch, an OIE Expert. In addition, a packing of specimen using standard container
                   approved by IATA has been demonstrated and practiced by all participants to have an experience and
                   discuss on constrain of airline shipment. The protocol sample submission to the reference laboratory
                   has been prepared and distributed to all member countries to use as a guidance.

                  The second round of 2011_ inter-laboratory comparison testing on foot and mouth disease (FMD)
                   ELISA typing, FMD serology by LP ELISA and NSPs test has been organized by RRLSEA,
                   Pakchong, Thailand during March to August 2011. Totally 17 FMD laboratories within Thailand and
                   SEACFMD countries were participated. The reports of 2011_inter-laboratory comparison has been
                   prepared and distributed to all participating laboratories and National Coordinators in August 2011
                   during the 14th National Coordinator Meeting of the SEACFMD in Hanoi, Vietnam during 12-16
                   August 2011.



Annual reports of OIE Reference Centres, 2011                                                                          3
                                                 Foot and mouth disease




     b)   Organisation of inter-laboratory proficiency testing with laboratories other than OIE
          Reference Laboratories for the same pathogens and diseases to ensure equivalence of
          results

                RRLSEA participated in Foot and Mouth Disease and Swine Vesicular Disease (SVD) Proficiency
                 studies 2011 by antigen capture ELISA, LP ELIA and NSP ELISA and RT-PCR test. The PT
                 programme was organized by WRL, Pirbright Laboratory, UK during August – October 2011. The PT
                 results has been sent to WRL since October 2011.

                RRLSEA has sent a panel of FMD samples annually to World Reference Laboratory (WRLFMD),
                 Pirbright Laboratory, UK. Totally 34 FMD samples were submitted to WRL in July 2011 for FMDV
                 serotype confirmation and strain characterisation, the diagnostic results were compared between
                 RRLSEA, Thailand and WRL, UK. Details of 34 samples were from Thailand = 17, Vietnam = 6, Lao
                 PDR = 4 and Sri Lanka = 2 respectively. The diagnostic results from both laboratories gave a
                 similarly results or indicted as 100 % agreement.


4.   Preparation and supply of international reference standards for diagnostic tests or vaccines and
     supply of international reference standards for diagnostic tests or vaccines

     Preparation of reference materials such as rabbit trapping antibodies, guinea pig detecting antibodies, inactivated
     FMD antigen, positive control serum and negative control serum for verification and validation assay in order to
     meet the standard of ISO 17025:2005 regulation. These reference materials were supplied to RRLSEA laboratory,
     8 regional laboratories within Thailand and OIE member countries, such as Cambodia, Myanmar, Vietnam, Lao
     PDR, Sri Lanka as already listed in the table 2 of item No. 2.


5.   Research and development of new procedures for diagnosis and control

     Several projects have been conducted in this year and in progress, as this follow:
     -    Collaboration with the RRLSEA, Pakchong, Thailand and Australian Animal Health Laboratory (AAHL,
          Geelong, Australia, entitled: Foot and Mouth Disease Risk Management for Australia and South East Asia,
          duration 5 years, from 2011 -2015.
     -    Development of real time PCR technique for foot and mouth disease diagnosis, now the test has been used as
          routine diagnosis for supporting the ELISA typing test.
     -    On going Serosurveillance of foot and mouth disease in elephants in Thailand (3 years project, 2010- 2012)
     -    Serosurveillance of foot and mouth disease in sheep and goat in Thailand (2 year project, 2012-2013)
     -    On going vaccine matching test (r-value) and molecular epidemiology analysis of foot and mouth disease
          viruses causing outbreak in Thailand and SEA region.


6.   Collection, analysis and dissemination of epizootiological data relevant to international disease
     control

     Not done.


7.   Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
     pathogen and the disease concerned

     There were several activities has been conducted annually in order to maintain the international standard of quality
     assurance system, Biosfety and biosecurity system. As this follow:

         To maintain the ISO /IEC 17025:2005 Certified on Veterinary Laboratory testing for FMD diagnosis in the
          scopes of Antigen typing test, LP ELISA test and Non-structural protein test, by conducting the internal audit
          or assessment once a year by Accreditation Boby in Thailand, other activities related to ISO 17025 regulation
          such as calibration of essential equipment, verify standard materials regularly, staff training and regularly
          update the quality manual document under the ISO 17025 standard.




4                                                                             Annual reports of OIE Reference Centres, 2011
                                                  Foot and mouth disease




            To maintain the biosafety and biosecurity system in the laboratory by conducting the annual maintenance of
             BSL-3 microsecurity system such as testing of HEPA Filter efficacy, negative pressure system, Air-locked
             system, Pneumatic Logic Control (PLC) system, Exhaust system, Bio-waste treatment system, checking of 2-
             door autoclave and also staff training in Biosecurity system regularly.

            Participating in the Biorisk Management Programme organized by the Department of medical Sciences,
             Ministry of Public Health, This activity is to establish and implement the biosafety and biosecurity in the
             laboratory for animal health and human health in Thailand to work together in order to establish the One
             Health Concept in near future.


8.   Provision of consultant expertise to OIE or to OIE Member Countries

     8.1 Dr Wilai Linchongsubongkoch, has been worked with Department of Livestock Development (DLD) for the
         contribution and review of text book on OIE manual of diagnostic tests and vaccine for Terrestrial animals on
         chapter 2.1.5 Foot and Mouth Disease and Terrestrial Animal Health Code.

     8.2 Dr Wilai Linchongsubongkoch, has been attending the OIE Commission and Ad Hoc Group on Biosafety
         and Biosecurity in Veterinary Laboratories, the AHG worked on reviewing the OIE Terrestrial manual of the
         chapter 1.1.1 ‘ Collection and shipment of diagnostic specimens’ and chapter1.1.2 ‘Biosafety and biosecurity
         in the veterinary microbiology laboratory and animal facilities’ at OIE Headquarters during 19 -21 September
         2011.
     8.3 Dr Wilai Linchongsubongkoch has been attending OIE Sub-Commissions annual meeting for foot and mouth
         disease control campaign in South East Asia and China (SEACFMD) and others OIE meeting as a technical
         resource person.

     8.4 Dr Wilai Linchongsubongkoch has been attending the second project Steering Committee for phase II on
         Transboundary Animal Disease Control for Poverty Reduction in the Greater Mekong Subregion
         (GCP/RAS/233/ASB) as an technical resource person, at FAO Office, Bangkok, Thailand during 19 -30 June
         2011.

     8.5 Dr Wilai Linchongsubongkoch has been attending the meeting of technical committee of National Bureau of
         Agriculture Commodity and Food Standard (ACFS), Thailand on reviewing the standard procedure of foot
         and mouth disease diagnostic manual, during July – September 2011.


9.   Provision of scientific and technical training to personnel from other OIE Member Countries

     9.1 Training of RRL staff in 2011
     9.1.1      Total 4 persons have been train on Internal audit for ISO 17025:2005, during 22-23 February 2011
     9.1.2      Total 5 persons have been trained on Statistic for Analysis testing, during 8-10 March 2011
     9.1.3      Total 5 persons have been train on Analysis Instrument Certification, on 19 April 2011
     9.1.4      Total 4 persons have been trained on Research methodology on 24 March 2011
     9.1.5      Total 5 persons have been trained on Knowledge Management (KM). During 10-11 May 2011
     9.1.6      Total 8 persons have been trained on Biosafety and Bosecurity during 23-14 June 2011
     9.1.7      Dr Kingkard Boonsuya Seeyo has been participated in Laboratory Training Course on FMD in Cattle at
                Yunan Animal Science and Veterinary Institute, Yunan province, P.R. China, during 9-10 June 2011. The
                training under the FAO-ADB supported.
     9.1.8      Dr Kingkarn Boonsuya Seeyo has been trained on Laboratory Biorisk Management Awareness and
                Transportation of Infectious Substance in July 2011.
     9.1.9      Dr Kingkarn Boonsuya Seeyo was attending the FMD vaccine matching training course at World
                Reference Laboratory (WRL), Pirbright, UK, during 7-11 October 2011.
     9.1.10     Mr. Jarouy Yothakeaw received training on Advance Biorisk Training at Australian Animal Health
                Laboratory(AAHL), Geelong, Australia during 21-25 November 2011, the training was Supported by
                FAORAS and USAID.

     9.2 FMD Training to OIE member in 2011
     9.2.1      Total 8 persons from OIE –SRR, Bangkok has visited RRLSEA laboratory and the BSL-3 facilities also
                there was a discussion and exchange FMD information.



Annual reports of OIE Reference Centres, 2011                                                                         5
                                                Foot and mouth disease




    9.2.2   Two trainees from Sri Lanka, Dr Eranga Abeyratna and Mr. Ha Wijithasiri, from Research Institute,
            Department of Animal Production and Health, Sri Lanka received training on FMD vaccine production,
            quality control and FMD diagnosis, during 17 January 2011 to 28 February 2011.
    9.2.3   Two laboratory staff from Department of Livestock and Fisheries (DLF), Lao PDR received training on
            Bio-safety Management in Laboratory for 2 weeks, during 1-12 August 2011. The training was under
            FAO supported.
    9.2.4   Total 5 Veterinary officers and with Scientists from Yunan Animal Science and Veterinary Institute,
            Yunan province, P.R. China received training on FMD diagnosis for 1 week during 29 August to 2nd
            September 2011 under the project on The Exchange of diagnostic techniques for transboundary animal
            disease.

10. Provision of diagnostic testing facilities to other OIE Member Countries

    RRLSEA have provided the diagnostic services to OIE member countries on antigen typing test, LP ELISA, NSP
    test, RT-PCR, strain characterisation by r-value and sequencing test, details as this follow.


      Country          Type of specimen                        Test method                    Total samples

      Cambodia         Epithelium tissue and tissue            Antigen typing, RT-PCR         31
                       extraction fluid                        r-value and sequencing
                       Serum sample
                                                               LP ELISA and NSP               80

      Lao PDR          Isolate virus                           Antigen typing, RT-PCR         20
                                                               and sequencing

      Vietnam          Epithelium tissue and Isolate virus     Antigen typing, RT-PCR         27
                                                               r-value and sequencing

      Sri Lanka        Epithelium tissue and Isolate virus     Antigen typing, RT-PCR         4
                                                               and sequencing


11. Organisation of international scientific meetings on behalf of OIE or other international bodies

    The 6th SEACFMD Laboratories Network and joint FMD EpiNet Meeting was co-organized with OIE-SRR under
    the support of OIE-SRR at Pakchong, Nakhonratchasima, Thailand during 2-3 March 2011.


12. Participation in international scientific collaborative studies

    12.1 The RRLSEA has been conducted cooperative work and exchange FMD diagnostic information and samples
         with World Reference Laboratory for FMD (WRL), Pirbright, UK by sending a panel of FMD specimens and
         isolation samples to WRL annually. This year totally 34 samples were sent to WRL in July 2011 for the
         confirmation of typing ELISA and strain characterisation by r-value and sequencing.
    12.2 The RRLSEA participated in 2011 External Quality Assurance Exercises for proficiency testing in serology
         for foot-and-mouth disease. The programme was organized by Dr Jef Hammond, WRL, Pirbright Laboratory,
         UK during August –October 2011. A panel Non-infectious unknown samples (6 samples), were received for
         antigen detection as well as a panel of non-infectious sera for serology test by LP ELISA and NSP test (6
         samples), The results was sent to WRL by October 2011 and final report is on-going evaluated.
    12.3 The RRLSEA organized the inter-laboratory comparison programme for FMD serology test and antigen
         detection, Totally 17 laboratories were participated in this programme exercise, which were 9 Laboratories
         from South East Asia countries and 8 FMD Laboratories within Thailand. A set of non-infectious reagents
         consist of 5 unknown serum samples and 10 unknown inactivated agents were distributed on date 3 rd March
         2011 to all participating laboratories to use for LP ELISA, NSP test and antigen typing test respectively. The
         inter-laboratory samples has been analysed, the completed report has been prepared and distributed to all
         participating laboratories within September 2011
    12.4 On-going collaboration with the RRLSEA, Pakchong, Thailand and Australian Animal Health Laboratory
         (AAHL, Geelong, Australia, entitled: Foot and Mouth Disease Risk Management for Australia and South



6                                                                           Annual reports of OIE Reference Centres, 2011
                                                 Foot and mouth disease




           East Asia, duration 5 years, from 2011 -2015.


13. Publication and dissemination of information relevant to the work of OIE (including list of
    scientific publications, internet publishing activities, presentations at international conferences)

         Presentations at international conferences and meetings
     -     Linchongsubongkoch, L. was attended the final Thailand joint coordinating committee meeting (JICA
           ADB2-Thailand project), 14 January 2011, Bangkok, Thailand.
     -     Linchongsubongkoch, L. was attended the One Health Congress, 14-16 February 2011, Melbourne,
           Australia.
     -     Linchongsubogkoch, L. was invited to give presentation in the 6th SEACFMD Laboratories Network and
           joint FMD EpiNet meeting, Pakchong, Nakhonratchasia, Thailand during 2-3 March 2011.
     -     Linchongsubogkoch, L. was invited to give presentation and attending the 17th Meeting of the OIE Sub-
           Commission for Mouth Disease Foot and Mouth Disease in South East Asia, Bali, Indonesia, during 7-11
           March 2011.
     -     Aunpomma, D., Thongtha, P. and Seeyo Kingkarn Boonsuya were attended the 6th SEACFMD Laboratories
           Network and joint FMD EpiNet meeting, Pakchong, Nakhonratchasia, Thailand during 2-3 March 2011.
     -     Aunpomma, D. and Seeyo Kingkarn Boonsuya were attended in the 17th Meeting of the OIE Sub-
           Commission for Mouth Disease Foot and Mouth Disease in South East Asia, Bali, Indonesia, during 7-11
           March 2011.
     -     Linchongsubongkoch, L was invited to give presentation in STANDZ Launching and Steering Committee,
           STANDZ Monitoring and Evaluation Workshop and 14th meeting of SEACFMD National Coordinators
           (NC), Hanoi, Vietnam during 12-16 September 2011.
     -     Linchongsubongkoch, L was invited to attend the OIE Commission and Ad Hoc Group on Biosafety and
           Biosecurity in Veterinary Laboratories, OIE, Paris during 19-21 September 2011.
     -     Dr Panithan Thongtha and Dr Kingkarn Boonsuya Seeyo were invited to give a presentation on the 3rd
           Central Laboratory Directors Meeting, KL, Malaysia 18-21 October 2011.
     -     Linchongsubogkoch, L. And Dr Kingkarn Boonsuya seeyo were participated and given the presentation in
           The 7th OIE/FAO FMD Reference Laboratories Network Meeting, Pirbright laboratory, UK, during 13-16
           November 2011. Organized by OIE/FAO.
     -     Linchongsubogkoch, L. was invited to give presentation in the Inception meeting of the OIE/JTF project for
           FMD control in Asia, Tokyo, Japan, 13-14 December 2011.

         Scientific publications in peer-reviewed journals

     -     Linchongsubongkoch, L., Aunpomma, D., Singkleebuth, S., Samanit, J., Chareonpol, P. and Thongtha, R.
           2011. Quality assurance for foot and mouth disease laboratories network. Annual technical meeting of the
           National Institute of Animal Health, Surin province, Thailand during 21-24 June 2011.

         Scientific publications in peer-reviewed journals

     -    Linchongsubongkoch was requested from OIE to review scientific paper for publishing in OIE journal
          entitled ‘ Epidemiology of foot and mouth disease in Uttar Pradesh state, India 2000-2009’ Authors by Amit
          Kumar Verma, Sahzad and S. K. Yadav, Department of Veterinary Epidemiology and Preventive Medicine,
          DUVASU, Mathura, Uttar Pradesh, India

         Other communications
     -     Technical consultant and assistant on FMD subjects to universities and institutes both in country and OIE
           member countries such as analysis and validation assay on project titled Development of recombinant 3ABC
           non-structural protein based ELISA to differentiate vaccinated from infected animals
     -     Consultant and gave advices to FMD laboratories within Thailand and OIE member countries on FMD
           diagnostic testing and quality assurance control system also other subjects related to FMD.
     -     Assist and coordinate with OIE member countries (Myanmar, Cambodia, Lao PDR and Vietnam) in
           preparing an essential document, packing of infectious substances and transportation of specimen to
           Reference laboratory by International Airline complied with IATA regulation and using standard container
           approved by UN or IATA.
                                                    _______________




Annual reports of OIE Reference Centres, 2011                                                                      7

				
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