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					  Acute toxicity to some
g A                          state Michaelis–Menten kinet   tics.7
   ish
  fi and aquatic             for the wild type isoform of 4-  -coum
   n t b t
  invertebrates              bottleneck and significantly s slowi
                             backup of cinnamic acid (Fig. 3). O
  Highly toxic to bees and
g H                          potential solutions to get around t
                                                             e
                             concentration of the wild type isof
   ome species of fish
  so        i    f fi h      2) To use a mutant enzyme w   with m
                             (Fig. 5) or 3) To 10x increase the c
                                                        (Fig. 6).
                             4-coumarate:CoA ligase (Fig. 6) W
                             coumarate:CoA ligase, the ba   ackup
                             cinnamaldehyde (4). The mut    tant is

 l
al                           selected for based on a 2000 pape
                             Institute who altered three po
                             and discovered the Km of 4-c
                                                            olar a
                                                            coum
                                              mutant s
                             increasing the mutant's subs   strate
                             concentration of the mutant i   isofor
                                                            en
                             increased 10x, the flux is eve mo
   yde
dehy production in:          most ideal situation for high y  yield
                             We chose to investigate all th hree o
                             conceived reaction kinetics w  were o
                             hypothetical model for the reverse
                             real world data exists charact   terizi
                             aforementioned enzymes. This me

				
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posted:4/3/2013
language:English
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