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					                         ANU Bioscience Services
                                 CMBE

                      Standard Operating Procedure
                                  004
                               Version 2.0
                               Uncontrolled if printed



Effective From : 30 Nov 2010                      Review due: 29 June 2011

References:
Reporting of illness. Injuries and abnormalities in animals
Related to: All personnel required to work with animals within an ABS Animal
Facility


TITLE:               Using Animal Monitoring Score Index & Cards


OBJECTIVE

To ensure that any animal identified as “At risk” in a welfare capacity is adequately
monitored to avoid undue pain, suffering or death of the animal.

BACKGROUND

Monitoring score cards are to be used for animals which have been identified as having
an illness, disease, phenotype or undergoing a procedure that may impact the welfare of
the animal.

RISK STATEMENT:

As this is an administrative procedure there has been no physical safety risks associated
with it.

PPE

There is no additional PPC&E required for this procedure

PREREQUISITES

      Competent in identifying sick and abnormal animals

EQUIPMENT REQUIRED
   Writing implements
   Score cards
                       Standard Operating Procedure
               004 - Using Animal Monitoring Score Index & Cards
                                       Version 2.0


PROCEDURE

DEVELOPING THE SCORING INDEX:
1. A Scoring Index must be developed where the Protocol indicates that an animal is “At
risk” in a welfare capacity. It is the responsibility of the Chief Investigator to ensure that
the Index is available and approved by the AEEC. The Index must include:
      Normal basic physical characteristics
      Expected changes to physical characteristics based on procedures performed
   Refer to example below
2. Where an animal is not part of a Protocol that has been identified as requiring a
specific scoring index by the AEEC, a Generic Scoring Index is available, see below
3. The Scoring Index must be available in all rooms where affected animals are kept.


USING THE SCORING INDEX:
   1. Under each Observable:
             Identify the description that best applies to the condition of the animal and
              note the relevant column number.
             Record the appropriate score on the card/sheet against that observable.


COMPLETING THE CARD/SHEET:
NOTE: New cases must be flagged with a red cage card so the facility veterinarian
knows to examine the animal during rounds (Please refer to the procedure “Reporting of
Sick & Abnormal Animals”)

   1. Immediately upon identifying an abnormality or sickness, complete an “Animal
       Monitoring Score Card/Sheet”, ensuring the following is included:
       o Animal details and date first observed.
       o Note the Examination interval, eg: Daily/Weekly.
       o Score the animal according to the clinical signs observed. (Refer to the
          scoring index which is available in every room)
       o Cross out observables that are not applicable, eg: surgery/site wound
   2. The information is to be readily available either on a card attached to the animal
       cage or in a folder within the relevant animal room. Where the information is
       contained in a folder, the cage must be noted to direct observers to check and
       score the animal.
                              UNCONTROLLED IF PRINTED
                           Standard Operating Procedure
                   004 - Using Animal Monitoring Score Index & Cards
                                          Version 2.0



SCORING OUTCOMES
   1.      If an animal reaches a score of ‘3’ on any given examination interval, it is to be
           euthanased immediately. This could be either “3” in one observable or a
           cumulative score across a number of observables at that exam, eg: 1+1+1, or
           2+1. Ensure a record is made on the card/sheet as to the reason behind its
           euthanasia.
   2.      If an animal has 3 consecutive exam intervals scoring a total of ‘0’, cease
           monitoring the animal and record the information on the card/sheet.
NOTE: Where an exam interval will fall “Out of Hours” relevant staff must be notified to
ensure the animals are checked.
   3.      Score Cards/Sheets must be kept for future reference for the researcher or
           facility veterinarian.
   4.
EXAMPLE SCORE CARD/SHEET
                          ANIMAL OF INTEREST: MONITORING CARD
        Animal Details:       ENU 101 Spot    First Noted: (date)               10/03/2010
                                 Spotty,
        Phenotype:              paralysis     Exam Interval:                       Daily
                                                             Date
        OBSERVABLES           10/03   11/03   12/03     13/03       14/03   15/03
        Coat                  0       0       0         0           0       1
        Activity              0       0       1         1           1       1
        Breathing
        Movement              0       0       0         1           1       1
        Eating/Drinking
        Condition
        Dehydration
        Faeces/Urine
        Body Weight
        Surgery
        Site/Wound
        Comments



                                                                            Culled
        INITIALS              SF      SF      SF        SF          SF      SF




                                  UNCONTROLLED IF PRINTED
                          Standard Operating Procedure
                  004 - Using Animal Monitoring Score Index & Cards
                                           Version 2.0


VERSION HISTORY

Version           Date Initiated    Changes Made                                    Made by
Number
1.0               23 Nov 2010       Original
2.0               April 2011        Addition of new example score card
                                    Minor formatting and editing changes




SUMMARY

         All animals that are identified as being “At Risk” must have a monitoring score
          card/sheet available for review in the room
         Ensure animals are checked at the correct intervals as indicated
         If an animal reaches a score of ‘3’ on any given examination interval, it is to be
          euthanased immediately. This could be either “3” in one observable or a
          cumulative score across a number of observables at that exam


I have read the full version of the above summarised SOP and recognise the risks
associated with this procedure.


Name
                                                           Date

Signature


Supervisor
                                                           Date

Signature


A copy of this summary page must be kept in each employee’s handbook.




                                   UNCONTROLLED IF PRINTED
                                         Example of Scoring Index


                                           ABS GENERIC SCORING INDEX
                                                                    SCORES
OBSERVABLES             0                       1                                2                           3
                                                                                                       Discoloured,
Coat                 Normal                   Rough                   Unkempt, thin, wounds           skin discharge
                                                                       Huddled/inactive OR
Activity             Normal         Isolated, abnormal posture               overactive           Declining or seizuring
                                                                      Rapid, deep (abdominal     Laboured, cyanosis (blue
Breathing            Normal               Rapid, shallow                    movement)                   extremities)
                                                                     uncoordinated, walking on    Staggering, paralysis,
Movement             Normal      Slightly uncoordinated/ abnormal     toes, reluctant to move         limb dragging
                                                                      Increased/decreased for    Increased/decreased >48
Eating/Drinking      Normal       Increased/Decreased for 24 hrs                48 hrs                 hours. Obese*
Condition            Normal                    Thin                     Loss of fat, no growth         Loss of muscle
Dehydration          Nil                 Skin less elastic                    Skin tents          Skin tents, eyes sunken
                                                                      Diarrhoea or dry faeces,    Uncontrolled diarrhoea,
                                                                           abnormal urine         blood in faeces. Nil urine
Faeces/Urine         Normal                Moist faeces                    (volume/colour)              or incontinent
                                                                                                   Weight loss >10% over
                                                                                                 short period or >20% over
Body Weight          Normal              Reduced growth                  Weight loss >15%                    time
                     Normal,                                           Discharge ++ or gap       Bleeding heavily, wound
Surgery Site/Wound   healing                Discharge                        forming                 reopened, red

                              If surgery has taken place, please indicate time, date and surgeon in comments section
 Other Information
                      Specific requests/comments regarding animal/strains should be recorded on the database and made by
                                                 email, these forms are ONLY for daily monitoring

				
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