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					Cloning and expression of the coding regions of th...[Biol Res. 2003] - PubMed Result                                                                        Page 1 of 1



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     1: Biol Res. 2003;36(3-4):421-8.                                                                                                                                   Links


                                                                                                                 Related Articles
  Cloning and expression of the coding regions of the heat shock proteins HSP10
  and HSP16 from Piscirickettsia salmonis.                                                                           Production and immune response of recombinant
                                                                                                                     Hsp60 and Hsp70 from the salmon pathogen
                                                                                                                     Piscirickettsia salmonis.           [Biol Res. 2005]
  Wilhelm V, Huaracán B, Martínez R, Rosemblatt M, Burzio LO, Valenzuela
  PD.                                                                                                                Isolation and expression of the genes coding for the
                                                                                                                     membrane bound transglycosylase B (MltB) and the
  Fundación Ciencia para la Vida e Instituto Milenio de Biología Fundamental y Aplicada, Av.                         transferrin binding protein B (TbpB) of the salmon
  Marathon 1943, Santiago, Chile.                                                                                    pathogen Piscirickettsia salmonis.      [Biol Res. 2004]

  The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon                                           Immunological characterization of a bacterial protein
                                                                                                                     isolated from salmonid fish naturally infected with
  pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10                                      Piscirickettsia salmonis.                [Vaccine. 2007]
  coding sequence is located in an open reading frame of 291 base pairs encoding
  96 aminoacids. The HSP16 coding region was isolated as a 471 base pair                                             Cloning and sequencing of the genes coding for the
  fragment encoding a protein of 156 aminoacids. The deduced aminoacid                                               10- and 60-kDa heat shock proteins from
  sequences of both proteins show a significant homology to the respective protein                                   Pseudomonas aeruginosa and mapping of a species-
                                                                                                                     specific epitope.              [Infect Immun. 1991]
  from other prokaryotic organisms. Both proteins were expressed in E. coli as
  fusion proteins with thioredoxin and purified by chromatography on Nicolumn. A                                     Putative heat shock protein 70 gene from
  rabbit serum against P. salmonis total proteins reacts with the recombinant                                        Actinobacillus actinomycetemcomitans: molecular
  HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using                                                                 [Oral Microbiol Immunol. 1998]
                                                                                                                     cloning and sequence analysis of its gene.
  serum from salmon infected with P. salmonis. The possibility of formulating a
  vaccine containing these two proteins is discussed.                                                                                            » See all Related Articles...

  PMID: 14631874 [PubMed - indexed for MEDLINE]


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