Design of Experiments and Statistics in Biotechnology Chemical

Design of Experiments and Statistics in Biotechnology Chemical Engineering / Biomedical Engineering Graduate Course Tufts University – Summer Term 2009 Title: Design of Experiments and Statistics in Biotechnology Course Number: CHBE/BME 193 - C Course Description Examples in the areas of cell culture, purification, formulation, drug delivery, and tissue engineering will be used to describe methods for designed experiments and statistical analysis of data. Attention will be on methods for collection and reporting of scientific and engineering data for research and publication. Brief review of and advanced treatments of statistical concepts of sample sizes, population numbers, ANOVA, and variances (BME-193 AT) will be included. Statistical and Taguci square designs will be included. Additional sample problems will be added from student requests and or samples of research data. Pre-Req’s and Overlap • This class is not a substitute for BME193AT (which is required in some Biomedical Engineering Degree Programs. • Overlap of 193C with 193AT is minimal (~1 hour over the term) • A college level (undergraduate or graduate) statistics class is recommended prior to ChBE/BME193C. Offered in : Summer2009 Day(s) : W 6-9:30pm Instructor : Leah C. Abraham Class Lecture 1 Lecture 2 Lecture 3 Lecture 4 Lecture 5 Lecture 6 Lecture 7 Lecture 8 Lecture 9 Lecture 10 Lecture 11 Lecture 12 Date 20may2009 Material Course Intro DOE and Protein Basics DOE Math Data Integrity Analysis Intro Software Experiment Design - Software Blocking / Adv. Analysis – software Adv. Designs: Latin Sq, Taguchi Data transformations Range and Response Optimization Data Smoothing / Process Control Final / Student Requests Recommended Readings (Montgomery, 2005) – Ch1, Ch5.1 (Montgomery, 2005)- Skim Ch 2,3,5 (Naveena et al., 2005) (Szalowska et al., 2007) (Montgomery, 2005)- More Ch 2,3,5 (Montgomery, 2005)- More Ch 2,3,5 (Chen et al., 2003) Worked Problems Due 27may2009 Cell culture doubling time PS 1 PS 2 03june2009 10june2009 17june2009 24june2009 01july2009 08july2009 15july2009 22july2009 29july2009 05aug2009 HPLC elution time variance PS 3 PS 4 / exam 1 (Montgomery, 2005)- More Ch 5 (Montgomery, 2005)- Ch 6,7 (Montgomery, 2005)- Ch 8 AFM roughness for TE scaffolds PCR transcript levels– error calc’s PS 5 PS 6 PS 7 PS 8 / exam 2 (Montgomery, 2005)- CH 9, 15 (Montgomery, 2005)- Ch10 (Haaland, 1989)- Ch. 9 … looking for a good review article Preformulation solubility study PS 9 PS 10 / projects (Montgomery, 2005)- Ch 11 Recommended Readings TE in vitro viability experiment PS 11 / final Leah Abraham 1 April 3, 2009 Design of Experiments and Statistics in Biotechnology Chemical Engineering / Biomedical Engineering Graduate Course Tufts University – Summer Term 2009 CHBE/BME 193 -C - Design of Experiments and Statistics in Biotechnology Instructor: Leah C Abraham Tufts University 4 Colby Street Medford, MA 02155 leah.abraham@tufts.edu 617-627-2445 Examples in the areas of cell culture, purification, formulation, drug delivery, and tissue engineering will be used to describe methods for designed experiments and statistical analysis of data. Attention will be on methods for collection and reporting of scientific and engineering data for research and publication. Brief review of and advanced treatments of statistical concepts of sample sizes, population numbers, ANOVA, and variances (BME-193 AT) will be included. Statistical and Taguci square designs will be included. Additional sample problems will be added from student requests and or samples of research data. BME193AT or equivalent statistics class. Exams (60% of grade) Problem Sets (20% of grade) Project (20% of grade) Primary text – Montgomery – Design and Analysis of Experiments 6th edition Wiley, 2004 http://www.amazon.com/Design-Analysis-Experiments-DouglasMontgomery/dp/047148735X/ref=pd_bbs_2?ie=UTF8&s=books&qid=1238735587&sr=82 Description: Prerequisites: Requirements: Texts: Project: Turnitin A 4-5 page paper/project addressing an issue particular to statistics/design of experiments is required. Suggestions for topics will be made in class. Students will select a proposed paper topic with problem set #2. Papers on approved topics are due at the beginning of lecture # 10. The papers grades will reflect both technical content and writing. The program “Turnitin” is planned for use to encourage good referencing on assignments. http://www.turnitin.com/ factor Per the Dean’s office: “Our hope is not just to catch students cheating, but to create a strong incentive to do work and to avoid the temptation to cheat.” Ref: Jillian L. Dubman / Anti-Plagiarism Information / Sep 1, 2006 11:01 AM class ID: 1894797 enrollment password: Leah Abraham 2 April 3, 2009 Design of Experiments and Statistics in Biotechnology Chemical Engineering / Biomedical Engineering Graduate Course Tufts University – Summer Term 2009 Grading • Exams are similar to the problem sets. The exams have a short answer/general understanding section that are closed book. The more involved and calculation problems are open book / open notes. The "oral" final was a big hit with the last group how took the class. Grades ran from 85100%. It was a long set of short answer questions from which people got to select the ones they wanted to answer. Extra points were given for people who helped each other "correct" answers, and there were extra questions available in case people needed extra tries. I will offer an option to go with the final in written form, but the oral is much more fun. Undergrad’s are graded on the same scale as grad’s (you get the same credit). • • A: A ≥ X + B: X + σ 2 30% 55% σ 2 ≥ B ≥ X −σ ≤ 1% C : X − σ ≥ C ≥ X − 2σ 15% D : D ≤ X − 2σ Leah Abraham 3 April 3, 2009