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Table st1_ XLSX - mBio

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Table st1_ XLSX - mBio Powered By Docstoc
					Supplementary Table 1A. M. sympodialis proteins involved in cell wall bio
                   S. cerevisiae

β-1,3-glucan synthesis and processing
β-1,3-Glucan synthesis
       Fks family          YLR342W and others
         Rho1                  YPR165W




          Knr4              YGR229C
          Hkr1              YDR420W
β-1,3-Glucan processing
          Eng1              YNR067C
    Exg1, Exg2, Spr1    YLR300W, YDR261C,
                            YOR190W




        No hits                    No hits

        Bgl2                   YGR282C
      Crh family           YGR189C and others

      Gas family           YMR307W and others
      Sun family           YHR200W and others
       No hits                  No hits

Chitin synthesis and processing
UDP-N-acetylglucosamine synthesis (LeLoir pathway)
        Gfa1                 YKL104C
        Gna1                 YFL017C

         Pcm1                  YEL058W
          Qri1                 YDL103C
Chitin synthesis
         Chs1                  YNL192W

         Chs2                  YBR038W

         Chs3                  YBR023C
Chitin synthesis accessory proteins
         Chs4                   YBL061C


        Chs5                  YLR330W
        Chs6
                             YJL099W
         Chs7                YHR142W
Chitinases
         Cts1                YLR286C
         Cts2                YDR371W
N-acetyl-β-D-glucosaminidase
        No hits               No hits

Chitosanase
        No hits                No hits
Chitin deacetylases
         Cda1                 YLR307W




β-1,6-glucan synthesis and processing
β-1,6-glucan synthesis
         No hits               No hits

         Kre1                 YNL322C
         Kre5                 YOR336W
         Kre6                 YPR159W




        Kre9                  YJL174W
        Kre11                 YGR166W

         Big1                 YHR101C
Other enzymes for cell wall synthesis
        Ecm33                 YBR078W
      Dfg5/Dcw1               YMR238W

Cell wall integrity signalling
        Wsc1                  YOR008C


         Pkc1                 YBL105C
                        YJL095W
     Mkk1, Mkk2     YOR231W, YPL140C
        Slt2            YHR030C
       Mcm1             YMR043W
       Rlm1             YPL089C

    Rom1, Rom2      YGR070W, YLR371W
      Cdc24             YAL041W
      Bem2              YER155C

        Lrg1            YDL240W
        Sac7            YDR389W
Protein glycosylation
O-mannosylation
       Pmt1             YDL095W
       Pmt2             YAL023C
       Pmt4             YJR143C
       Mnt1             YDR483W
       Alg1             YBR110W
       Alg2             YGL065C



        Alg3            YBL082C
        Alg5            YPL227C

        Alg6            YOR002W
        Alg7            YBR243C

        Alg8            YOR067C
        Alg9            YNL219C

       Alg11            YNL048W

       Alg12            YNR030W
       Anp1             YEL036C
       Die2             YGR227W

       Mnn1             YER001W
       Mnn3             YDR372C
       Mnn4

       Mnn9             YPL050C
       Van1             YML115C
       Mnn10            YDR245W
       Mnn11            YJL183W
        Ost1             YJL002C
        Ost3            YOR085W
         Och1, Hoc1           YGL038C, YJR075W
           Pmr1                   YGL167C
            Rot1                  YMR200W

            Stt3                   YGL022W
           No hits                  No hits
GPI biosynthesis pathway
           Cwh43                    YCR017C

            Eri1                   YPL096C-A
            Gaa1                    YLR088W
            Gab1                    YLR459W
            Gpi1                    YGR216C

            Gpi2                    YPL076W

            Gpi8                   YDR331W

            Gpi10                   YGL142C

            Gpi11                  YDR302W
            Gpi12                  YMR281W

        Gpi13, Las21           YLL031C, YJL062W
           Mcd4                    YKL165C
           Gpi14                   YJR013W
           Gpi15                   YNL038W
           Gpi16                   YHR188C

            Gpi17                  YDR434W

            Gpi18                   YBR004C

            Gpi19                  YDR437W

            Gwt1                    YJL091C
            Pbn1                    YCL052C

            Per1                    YCR044C
            Smp3                    YOR149C
            Spt14                   YPL175W

1
    M. globosa and U. maydis genes on the same rows are orthologs of M. sympodialis based on best-blast scores. M

Supplementary Table 1 B. M. sympodialis and M. globosa predicted GPI proteins
 Gene identifier Screening method2
   (Swiss-Prot
     number)
M. sympodialis predicted GPI proteins
   MSY001_1515            1+2
   MSY001_0697             1
   MSY001_1070            1+ 2
   MSY001_0245            1+ 2
   MSY001_2789            1+ 2
   MSY001_2778            1+ 2
   MSY001_1638            1+ 2
   MSY001_2913            1+ 2
   MSY001_2552             2

   MSY001_2190             2
M. globosa predicted GPI proteins
     MGL_0131             1+2
  (XP_001732356.1)
     MGL_0132             1+2
  (XP_001732357.1)
     MGL_0200              1
  (XP_001732425.1)
     MGL_0288              2
  (XP_001732513.1)
     MGL_0504              2
  (XP_001732729.1)
     MGL_0599             1+2
  (XP_001732824.1)
     MGL_0610              1
  (XP_001732835.1)
     MGL_0611             1+2
  (XP_001732836.1)
     MGL_0663             1+2
  (XP_001732070.1)
     MGL_0751             1+2
  (XP_001732158.1)
     MGL_0798              1
  (XP_001732205.1)
     MGL_1187             1+2
  (XP_001731919.1)
     MGL_1308              1
  (XP_001732040.1)
     MGL_1399             1+2
  (XP_001731216.1)
     MGL_1565              1
  (XP_001731382.1)
        MGL_1575                        1
    (XP_001731392.1)
        MGL_1981                      1+2
    (XP_001730982.1)
        MGL_2668                      1+2
    (XP_001730286.1)
        MGL_3439                      1+2
    (XP_001729404.1)
        MGL_3490                      1+2
    (XP_001729455.1)
2
  Method 1: Big-PI positive, SignalP4 positive and no internal TM domains. Method 2: pattern + composition scannin
3
  Orthologs predicted to be GPI proteins are in bold.
    sympodialis proteins involved in cell wall biosynthesis and remodeling, and thei
                                     Description

cessing

          Catalytic subunit β-1,3-glucan synthase complex, ScFks1-3
          Rho-related GTPase, ScRho1-like




          Regulatory component β-1,3-glucan synthesis
          Regulatory component β-1,3-glucan synthesis

          Endo-β-1,3-glucanase (GH 81-family)
          Exo-β-1,3-glucanase family (GH 5 family); ScExg1-2, ScSpr1




          Exo-β-1,3-glucanase (GH55-family); related to Coniothyrium
          minitans exo-β-1,3-glucanase (Cmg1)
          β-1,3-glucanosyltransferase (GH17)
          Putative transglycosidases (GH 16); involved in crosslinking β-
          glucan and chitin
          β-1,3-transglucosylases (GH 72)
          Involved in septation, possibly β-glucosidase activity
          Mlg1 mixed-linked glucanase precursor [Cochliobolus carbonum]

g
Loir pathway)
          Glutamine-fructose-6-phosphate transaminase
          Glucosamine-phosphate N-acetyltransferase

          Phosphoacetylglucosamine mutase
          UDP-N-acetylglucosamine pyrophosphorylase;

          Class I chitin synthase

          Class II chitin synthase

          Class IV chitin synthase
          Activator of Chs3


          Component of exomer complex
          Component of exomer complex

          Export control protein

          Class III chitinase (GH18)
          Class V chitinase (GH18)

          N-acetyl-β-D-glucosaminidase (GH20); homologous to C. albicans
          Hex1

          A. oryzae and A. fumigatus chitosanases

          Putative chitin deacetylase




cessing

          Putative β-1,6-glucan synthases of Bradyrhizobium ; ndvB, ndvC

          Cell wall glycoprotein involved in β-glucan assembly
          Putative protein involved in β-1,6-glucan biosynthesis
          Putative transglycosidase required for β-1,6-glucan biosynthesis




          Glycoprotein involved in cell wall β-glucan assembly
          Role in cell wall β-glucan biosynthesis and the stress response

          Required for normal content of cell wall β-1,6-glucan
thesis
          Involved in cell wall biosynthesis but unknown function
          Endo-mannanase family (GH76) with a putative role in GPI-CWP
          incorporation

          Putative plasma membrane sensor-transducer of the stress-
          activated PKC1-MPK1 kinase pathway involved in maintenance of
          cell wall integrity
          Protein kinase C
MAPKKK
MAPKK
MAPK
MADS-box transcription factor
MADS-box transcription factors

Rho1-Guanine Exchange Factor
Guanine nucleotide exchange factor for Cdc42
Rho GTPase activating protein

GTPase-activating protein (GAP); ScLrg1
GTPase activating protein (GAP) for Rho1



Protein O-mannosyltransferase
Protein O-mannosyltransferase
Protein O-mannosyltransferase
Protein O-mannosyltransferase
Mannosyltransferase
Related to α-1,3-mannosyltransferase



α-1,3-Mannosyltransferase
Putative glucosyltransferase

Putative glucosyltransferase
UDP-N-acetyl-glucosamine-1-P transferase

Putative glucosyltransferase
Mannosyltransferase, involved in N-linked glycosylation
Mannosyltransferase, involved in N-linked glycosylation
Ortholog(s) have α-1,2-mannosyltransferase activity, role in
oligosaccharide-lipid intermediate biosynthetic process
α-1,6-Mannosyltransferase localized to the ER
Subunit of the α-1,6 mannosyltransferase complex
Ortholog(s) have dolichyl-phosphate-glucose-glycolipid α-
glucosyltransferase activity
Mnn family of α-mannosyltransferases (GT71)
Predicted Golgi α-1,2-mannosyltransferase
Protein required for normal mannosylphosphorylation of
oligosaccharides linked to cell wall proteins
Subunit of a Golgi mannosyltransferase complex (GT62)
Member of Mnn9p family of mannosyltransferases
Subunit of a Golgi mannosyltransferase complex (GT34)
α-1,6-mannosyltransferase activity
Putative oligosaccharyltransferase
Related to OST3 - oligosaccharyltransferase gamma subunit
           α-1,6-Mannosyltransferase
           High affinity Ca2+/Mn2+ P-type ATPase; ScPmr1
           ER chaperone involved in protein N- and O-glycosylation

           Putative oligosaccharyltransferase complex component
           β-Mannosyltransferases; CaBmt family

           Putative sensor/transporter protein involved in cell wall biogenesis

           ER membrane protein that binds and inhibits Ras2p
           Subunit of the GPI:protein transamidase complex
           GPI transamidase subunit
           Membrane protein involved in the synthesis of GlcNAc-PI

           Protein involved in the synthesis of GlcNAc-PI

           ER membrane glycoprotein subunit of the GPI transamidase
           complex
           Integral membrane protein involved in GPI anchor synthesis,
           putative α-1,2-mannosyltransferase
           ER membrane protein involved in GPI anchor assembly
           ER membrane protein involved in second step of GPI anchor
           assembly
           ER phosphoryltransferase for GPI anchor precursors
           Protein involved in GPI anchor synthesis
           Glycosylphosphatidylinositol α-1,4-mannosyltransferase I
           Protein involved in the synthesis of GlcNAc-PI
           Subunit of the glycosylphosphatidylinositol transamidase complex

           Transmembrane protein subunit of the glycosylphosphatidylinositol
           transamidase complex
           ER membrane glycoprotein subunit of the GPI transamidase
           complex
           Subunit of GPI-GlcNAc transferase involved in synthesis of
           GlcNAc-PI
           Protein involved in the inositol acylation of GlcN-PI
           Component of glycosylphosphatidylinositol-mannosyltransferase I

           Protein of the ER required for GPI phospholipase A2
           α-1,2-Mannosyltransferase involved in GPI biosynthesis
           UDP-glycosyltransferase subunit of the GPI-GnT complex
           mediates first step of pathway
ame rows are orthologs of M. sympodialis based on best-blast scores. Multiple genes in one row indicate paralogs in that spec

mpodialis and M. globosa predicted GPI proteins
                                    Description


roteins
          Putative chitin deacetylase
          Hypothetical protein
          MP88 [C. neoformans ]
          Phospholipase B
          Putative chitin deacetylase
          Aspartyl protease
          Hypothetical protein
          Putative chitin deacetylase
          N-term. part weak homology with C-term. part of GPI-anchored
          wall transfer proteins
          Flavin-binding monooxygenase-like protein
ins
          Hypothetical protein

          Hypothetical protein

          Hypothetical protein

          MP88 [C. neoformans ]

          Related to UDP-glucose:glycoprotein glucosyltransferase
          precursor (kre5)
          Aspartic protease

          Chitin deacetylase-like

          Chitin deacetylase-like

          Hypothetical protein

          Related to glucose oxidase

          Lipase

          Hypothetical protein

          Hypothetical protein

          Related to mixed-linked glucanase precursor MLG1

          Hypothetical protein
           Sphingomyelin phosphodiesterase

           MP88 [C. neoformans]

           Hypothetical protein

           Hypothetical protein

           Hypothetical protein

e and no internal TM domains. Method 2: pattern + composition scanning (De Groot & Brandt, FGB_2012) instead of Big-PI.
biosynthesis and remodeling, and their homologs in M. globosa and U. maydis 1
              M. sympodialis      Proposed Ms            M. globosa
                                     name


                MSY001_2709            Fks1          MGL_0311, MGL_0312
                MSY001_0082           Rho1.1             MGL_3754
                MSY001_1527           Rho1.2               No hits
                MSY001_0921           Rho1.3             MGL_1797
                MSY001_3465           Rho1.4             MGL_0548
                MSY001_0792            Knr4              MGL_1072
                  No hits                                  No hits

                  No hits                                   No hits
                MSY001_3300           Exg1.1               MGL_3222

                MSY001_3053           Exg1.2               MGL_2819
                MSY001_3396           Exg1.3
                MSY001_3180           Exg1.4         MGL_3944, MGL_3945
                MSY001_1797           Exg1.5
                MSY001_3407           Exg1.6
                MSY001_2105           Exg1.7             MGL_0748
                MSY001_1172            Msg1       MGL_2108 (+2107 and 2106)

                  No hits                                   No hits
                MSY001_3487            Utr2                MGL_4218

                  No hits                                  No hits
                  No hits                                  No hits
                MSY001_2330            Mlg1          MGL_1399, MGL_2744




                MSY001_0411           Gfa1                  MGL_2940
                MSY001_3342           Gna1       Present in the genome based on
                                                 TblastN, but no model available
                MSY001_1048           Pcm1                  MGL_1956
                MSY001_1326            Qri1                 MGL_0007

                MSY001_1791           Chs1                 MGL_4248

                MSY001_0691           Chs2                 MGL_1195

                MSY001_1876           Chs5          MGL_2139 + MGL_2138
                MSY001_2382           Chs6                 MGL_1466
                MSY001_1919           Chs8                MGL_2189_2
            MSY001_0030                   Chs7          MGL_3688

              MSY001_0295                 Chs4.1        MGL_1332
              MSY001_0220                 Chs4.2        MGL_2619
              MSY001_1921                 Chs4.3        MGL_2191
              MSY001_0276                  Chs5         MGL_2546
Present in the genome based on TblastN,                 MGL_4090
          but no model available
              MSY001_0705                 Chs7          MGL_1176

              No hits                                    No hits
            MSY001_0767                    Cts2         MGL_1104

                No hits                                  No hits


                No hits                                  No hits

            MSY001_2789                   Cda1.1   MGL_0610, MGL_0611

            MSY001_1515                   Cda1.2        MGL_3970
            MSY001_1517                   Cda1.3
            MSY001_2913                   Cda1.4        MGL_0816
            MSY001_2914                   Cda1.5



                No hits                                  No hits

              No hits                                    No hits
            MSY001_1494                    Kre5    MGL_0504 + MGL_0505
            MSY001_1559                   Kre6.1        MGL_3357

            MSY001_0543                   Kre6.2   MGL_0163; MGL_0162
            MSY001_3212                   Kre6.3       MGL_0376

                No hits                                  No hits
                No hits                                  No hits

                No hits                                  No hits

                No hits                                  No hits
                No hits                                  No hits



                No hits                                  No hits


            MSY001_0108                   Pkc1       MGL_2756+2756
              MSY001_2846                  Bck1          MGL_3142, MGL_0324
              MSY001_2857                  Mkk1          MGL_3129, MGL_3979
              MSY001_0128                   Slt2             MGL_2731
              MSY001_3483                  Mcm1              MGL_4212
Present in the genome based on TblastN,    Rlm1              MGL_2872
          but no model available
              MSY001_0240                  Rom1          MGL_2594, MGL_4005
              MSY001_2662                  Cdc24                MGL_0960
 Weak similarity with MSY001_2946 and      Bem2     Weak similarity with MGL_3683 and
              MSY001_0860                                       MGL_2818
              MSY001_1519                  Lrg1                 MGL_3892
   Only a hit with very weak similarity             Only a hit with very weak similarity



              MSY001_2013                  Pmt1               MGL_2299
              MSY001_3045                  Pmt2               MGL_2811
              MSY001_2301                  Pmt4               MGL_3853
              MSY001_2023                  Mnt1     MGL_2311, MGL_2312, MGL_2320
              MSY001_0066                  Alg1               MGL_3730
   MSY001_0809, also weak sim. with        Alg2               MGL_1050
  MSY001_0716 on the same scaffold
 (3.1), so possibly two copies in tandem
              MSY001_3019                   Alg3                 No hits
              MSY001_2232                  Alg5.1               MGL_2489
              MSY001_0419                  Alg5.2               MGL_2948
              MSY001_2045                   Alg6                MGL_2343
Present in the genome based on TblastN,     Alg7                MGL_3398
          but no model available
              MSY001_3196                   Alg8                MGL_0397
              MSY001_1844                  Alg9.1               MGL_3788
              MSY001_2930                  Alg9.2               MGL_4169
              MSY001_0716                  Alg11                MGL_1160

             MSY001_1982                   Alg12                MGL_2261
               No hits                                           No hits
             MSY001_2039                   Die2                  No hits

                No hits                                           No hits
                No hits                                           No hits
                No hits                                           No hits

                No hits                                          No hits
                No hits                                          No hits
                No hits                                          No hits
                No hits                                          No hits
            MSY001_2131                    Ost1                 MGL_0777
          Only very weak hits                                   MGL_2445
                          MSY001_3200                          Och1                      MGL_0392
                          MSY001_3092                          Pmr1                      MGL_3276
                          MSY001_3413                          Rot1                  MGL_0359, MGL_0666

                          MSY001_1830                           Stt3                       MGL_3807
                            No hits                                                         No hits

            Present in the genome based on TblastN,           Cwh43                          No hits
                      but no model available
                             No hits                                                        No hits
                          MSY001_1364                          Gaa1                        MGL_3621
                          MSY001_1143                          Gab1                         No hits
            Present in the genome based on TblastN,            Gpi1                        MGL_3923
                      but no model available
            Present in the genome based on TblastN,            Gpi2                        MGL_1215
                      but no model available
                          MSY001_0550                          Gpi8                        MGL_0172

                          MSY001_2444                          Gpi10                       MGL_1537

                            No hits                                                         No hits
                          MSY001_2666                          Gpi12                       MGL_0955

                          MSY001_1417                          Gpi13                       MGL_0416
                          MSY001_1018                          Mcd4                        MGL_1911
                          MSY001_2840                          Gpi14                       MGL_3150
                            No hits                                                         No hits
                          MSY001_3412                          Gpi16                       MGL_0360

                          MSY001_1734                          Gpi17                       MGL_2394

                          MSY001_1741                          Gpi18                       MGL_2402

                              No hits                                                        No hits

                          MSY001_2553                          Gwt1                        MGL_1672
                            No hits                                                         No hits

                          MSY001_1138                          Per1                        MGL_2065
                            No hits                                                         No hits
                          MSY001_2597                          Spt14                       MGL_1728

es. Multiple genes in one row indicate paralogs in that species, the orthologs (best scoring protein) are underlined
Cross-species ortholog3                          Comment



         No hits
        MGL_1187
        MGL_1981
        MGL_2589
        MGL_0610
        MGL_0599
        MGL_3439
        MGL_0816
        MGL_1672           The M. sympodialis gene has homology to only the C-term
                           of the M. globosa gene, which is not a GPI protein. Note
        MGL_4008           Ambiguous prediction.

         No hits           MGL_0132 seems 100% identical but has 14aa extra
                           behind the SP.
         No hits           MGL_0131 seems 100% identical but lacks 14aa behind
                           the SP.
         No hits

      MSY001_2728

      MSY001_1494

      MSY001_2778

      MSY001_2789

      MSY001_3514

      MSY001_1690

      MSY001_2109

 ?MSY001_2146 (MGL_0799)

      MSY001_0697

      MSY001_0602

      MSY001_2330

         No hits
                        MSY001_2467

                        MSY001_1070

                            No hits

                        MSY001_1638

                            No hits

nning (De Groot & Brandt, FGB_2012) instead of Big-PI.
d U. maydis 1
                  U. maydis



                  um01639
           um02494 (Rho2), um05734
                  um10663
               um00774 (Rac1)

                   um02733.2
                     No hits

                    No hits
          um00235, um00876, um05550,
              um04364, um10211
                   um01165

                    um06102


                    um10029




                um00891, um00330

                   um01640
                    No hits
          um05036, um02134, um04368,
                   um01898



                    um03199
                um10864, um11638

                    um04983
                    um11497

          um10117, um10120, um04290,
                  um10718.2


                   um10277.2
                    um10367
                    um03204
              um05480

              um10641
              um11518
              um10391
              um02840
              um04959

              um11089

               No hits
          um10419, um06190

              um00695




    um02019, um00638, um01788

              um05792

          um11922, um01143




No hits


um01512
um00857

um03569, um05807
um10364, um05809, um05811,
um15051, um05718
No hits
No hits

No hits

No hits
No hits




              um15023
          um01662
          um10855
          um10107
          um01224
        um10560 (weak)

   um15070 (closer to Rom2)
         um02422
         um01161

          um10200
um02902 (more similarity to Bag7)



          um11220
          um10749
          um05433
      um01154, um01821
          um04649
          um02653



            um06205
            um01231
            um06329
            um02519
            um10484

            um00605
            um06088
            um11758
            um05209

            um11341
             No hits
            um05547

             No hits
             No hits
             No hits

             No hits
             No hits
             No hits
             No hits
            um05769
            um04198
    um02624, um15072
         um04461
um11927, um11187, um04171,
         um10940
         um05293
          No hits

        um10320.2

          No hits
         um06205
         um11456
         um00213

          No hits

         um05709

         um06295

          No hits
         um00302

         um03469
         um00454
        um11347.2
          No hits
         um02731

         um01565

         um05554

          No hits

         um06316
          No hits

         um04859
          No hits
         um11784
Proposed Ms name



     Cda1.2
      Pga1
      Pga2
      Plb1
     Cda1.1
      Pga3
      Pga4
     Cda1.4
      Pga5

      Pga6
                                            Comment



MGL_0312 and 0311 are two parts of one gene.
MSY001_0082 best hit with ScRho1.




Homology in MGL_3944 start at aa 503, might be a fusion of two genes.



MGL_2108, 2107 and 2106 are three parts of one gene.


MGL_4218 is not a GPI protein.



MGL_1399 is a predicted GPI protein but MSY001_2330 is not. Based on pairwise Blast the latter lacks
its C-terminal region.TblastN indicates a 2nd Ms paralog but no model available.




Ms gene model may be combination of two ORFs. First part comprises part of a chitin synthase until but
not including the conserved QRRRW motif. Last part is highly similar to MGL_1196.
Based on Ustilago maydis nomenclature; MGL_2139 + MGL_2138 are two fragments of 1 gene.
Based on Ustilago maydis nomenclature.
Based on Ustilago maydis nomenclature.
Based on Ustilago maydis nomenclature.




MSY001_2789 seems to be a fusion/duplication of two tandem chitin deacetylase genes.
MSY001_2789, and MGL_0610 and 0611 are predicted GPI proteins.
Tandem duplication with MSY001_1517.
Tandem duplication with MSY001_1515.
Tandem duplication with MSY001_2914.
Tandem duplication with MSY001_2913.




MGL_0504 and 0505 are two parts of 1 gene.


This Ms ORF seems to contain two copies of Kre6 in tandem.




MGL_2756+2756 are two parts of 1 gene.
Ms ORF only has similarity with C-terminal part of Bck1.




Weak similarity.




Ms and Mg genes do not hit each other in reciprocal blasts, MSY001_2946 is ortholog of MGL_3555,
and MSY001_0860 is ortholog of MGL_0853.




Mg N-term seems to be missing.




Ms ORF is lacking C-term part according to tBlastn result.

Weak similarity.




Ms sim. only in C-term half, Mg N-term seems to be missing.
Ms N-term possibly missing.



Ms and Um weak similarity.




Ms, Mg weak similarity.
All have very weak similarity. Reciprocal blast of um02624 with Ms is negative.




um06205 also shares similarity with Alg3 and MSY001_3019.

Ms has weak similarity.

Very weak similarity.



MSY001_2444 seems to be a fusion of two ORFs. First part has homology with MGL_1537, 2nd part
with MGL_1539.




Only weak similarity with C-terminal half.

				
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