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Sphere_StrandDisplacementHandles.xlsx - OpenWetWare

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Sphere_StrandDisplacementHandles.xlsx - OpenWetWare Powered By Docstoc
					ORIGINALS TO BE MODIFIED (Red Strands in Sphere_StrandDisplacementHandles.json)

HANDLE 1      0[58]         0[59]
N. Hemisphere 2[140]        2[125]




HANDLE 2        22[425]     21[314]
S. Hemisphere
                21[286]     21[285]




NEW TO BE ORDERED
handle1      5' CGACTATACGATAACTTTTTTATTGCGTGCTTTCGAGGGGATTTTGCTAAGGA
handle1      5' CTTTCCAGACTTTCAACAGTTTCAAGGAACAACTAAAACAACGTTAGTTTTTTTCGATATGCTGTGCTACT
key1         5' GAACTGGAGTAGCACAGCATATCG TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT GTTATCGTATAGTCG

pseudohandle2   5'   CTAAAGCACAACAGTGCCA
handle2         5'   GTATCGGCGTCGAATTTTTTCGCTGTAAAACCCAGCCACAGACA
handle2         5'   AACAACTAATAGAATACGTGGAGAAGAAGAGCCATTTTTTTCGGGCATGTGCTACTCCAGTTC
pseudohandle2   5'   GCAGCAAATTAGGAGCACT
key2            5'   GAACTGGAGTAGCACATGCCCGATTTTTTTTTTTTTTTTTTTTTTTTTTTCGACGCCGATAC




NOT-TO-BE-MODIFIED ORIGINALS (Black Strands in Sphere_StrandDisplacementHandles.json)

                1[45]       2[156]
                2[62]       4[75]
                2[78]       2[63]
                2[96]       3[133]
                2[124]      4[174]
                2[155]      4[223]
                2[184]      2[185]
                3[50]       1[44]
3[134]   2[97]
3[210]   4[274]
4[51]    3[49]
4[74]    5[81]
4[101]   2[79]
4[127]   5[141]
4[173]   5[196]
4[200]   2[141]
4[222]   5[254]
4[273]   3[209]
5[82]    7[120]
5[142]   5[170]
5[171]   4[128]
5[197]   7[260]
5[255]   7[331]
5[283]   5[311]
5[312]   7[50]
6[49]    4[52]
6[81]    7[83]
6[114]   4[102]
6[146]   7[153]
6[181]   7[191]
6[210]   7[225]
6[243]   4[201]
6[275]   7[297]
6[310]   5[282]
6[340]   6[311]
7[51]    9[54]
7[84]    9[92]
7[121]   9[132]
7[154]   6[147]
7[192]   9[210]
7[226]   9[251]
7[261]   9[289]
7[298]   9[328]
7[332]   9[369]
7[367]   8[394]
8[161]   9[188]
8[393]   7[366]
9[33]    6[341]
9[55]    11[53]
9[73]     6[50]
9[93]     11[94]
9[112]    6[82]
9[133]    11[135]
9[151]    6[115]
9[189]    8[162]
9[211]    11[216]
9[230]    6[182]
9[252]    11[257]
9[270]    6[211]
9[290]    11[298]
9[310]    6[244]
9[329]    11[338]
9[348]    6[276]
9[370]    11[379]
9[391]    10[420]
10[192]   11[173]
10[419]   9[390]
11[54]    14[161]
11[95]    14[201]
11[136]   14[241]
11[174]   10[193]
11[217]   14[320]
11[258]   14[359]
11[299]   14[398]
11[339]   14[438]
11[380]   12[50]
11[421]   14[123]
12[49]    9[347]
12[90]    11[420]
12[131]   9[32]
12[171]   9[72]
12[212]   9[111]
12[253]   9[150]
12[296]   13[277]
12[334]   9[229]
12[375]   9[269]
12[416]   9[309]
13[51]    14[80]
13[278]   12[297]
14[79]    13[50]
14[100]   12[91]
14[122]   17[194]
14[141]   12[132]
14[160]   17[226]
14[180]   12[172]
14[200]   17[259]
14[218]   12[213]
14[240]   17[288]
14[259]   12[254]
14[281]   15[308]
14[319]   17[355]
14[337]   12[335]
14[358]   17[388]
14[377]   12[376]
14[397]   17[420]
14[415]   12[417]
14[437]   17[129]
15[77]    16[104]
15[309]   14[282]
16[103]   15[76]
16[138]   14[101]
16[172]   14[142]
16[209]   14[181]
16[244]   14[219]
16[278]   14[260]
16[316]   17[323]
16[349]   14[338]
16[386]   14[378]
16[419]   14[416]
17[130]   17[159]
17[160]   18[188]
17[195]   16[173]
17[227]   19[269]
17[260]   16[245]
17[289]   16[279]
17[324]   16[317]
17[356]   19[368]
17[389]   16[387]
17[421]   19[418]
18[158]   16[420]
18[187]   18[159]
18[215]   16[139]
18[273]   16[210]
18[299]   19[342]
18[328]   18[300]
18[388]   16[350]
19[197]   20[261]
19[248]   18[216]
19[270]   21[329]
19[297]   18[274]
19[343]   18[329]
19[369]   21[391]
19[396]   18[389]
19[419]   20[421]
20[260]   19[196]
20[336]   21[373]
20[420]   22[426]
21[315]   19[247]
21[330]   21[345]
21[346]   19[296]
21[374]   20[337]
21[392]   21[407]
21[408]   19[395]
23[412]   23[411]
ed Strands in Sphere_StrandDisplacementHandles.json)

           ATTGCGTGCTTTCGAGGGGATTTTGCTAAGGA
           CTTTCCAGACTTTCAACAGTTTCAAGGAACAACTAAAACAACGTTAGT




           CTAAAGCACAACAGTGCCACGCTGTAAAACCCAGCCACAGACA

           AACAACTAATAGAATACGTGGAGAAGAAGAGCCAGCAGCAAATTAGGAGCACT




GATAACTTTTTTATTGCGTGCTTTCGAGGGGATTTTGCTAAGGA
CTTTCAACAGTTTCAAGGAACAACTAAAACAACGTTAGTTTTTTTCGATATGCTGTGCTACTCCAGTTC
 TAGCACAGCATATCG TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT GTTATCGTATAGTCG

AACAGTGCCA
TCGAATTTTTTCGCTGTAAAACCCAGCCACAGACA
TAGAATACGTGGAGAAGAAGAGCCATTTTTTTCGGGCATGTGCTACTCCAGTTC
TAGGAGCACT
 TAGCACATGCCCGATTTTTTTTTTTTTTTTTTTTTTTTTTTCGACGCCGATAC




LS (Black Strands in Sphere_StrandDisplacementHandles.json)

           GAGCCTTTTTTTCACGTTGAAAATATAGAAGCGGAGTAACGAT                  43
           ACAACAACCAGCGAAAGACAGCAGCACCAACCTAA                          35
           AGTTGCGCTATCGGTTTATCAGCTAATAATAATTAATTGCGACAATG              47
           AAACAGCTTGGAGGCTTTGAGGACTAAAGATCAGGGATAG                     40
           AAATGAAAGGAACCCATGTACCCGCCACCCTCAGA                          35
           CTAAAGTAAACTACAACGCCTGATAGGTGTATCAC                          35
           CGGTCGCTGATCATAGTTAGCGTGAGACTCCAAAAAAAAGGCACCGATATATT        53
           TCGTCACCCTCAGCATCGCCCACGCATATCCAAAAG                         36
CAAGCCCAATTTTTCTGTATGTGAATTTCTT                       31
CAGACAGCCCGGCTTGCAGGGAGTTAAAGGCTTTGACCCCCAG           43
CTAAAACACTCATCCGCTTTTGCGGGA                           27
AACGAAAGAGGCACCATGTTACTTAGCC                          28
AATGCCACTACGAAGTCGGAACGAGGGTAGCAACGGCTACAATACCGAT     49
GTTTCCATTAAACGGGCAACTTTGAAAGAGGAC                     33
ACCGCCACCCTCCTATTATTCTGAAA                            26
GCCACCCTCAGAACGTAACACTGAGTTTCGTCACCAGTACTTTGTCGT      48
CGTACTCAGGAGGGATTAGCGGGGTTT                           27
CGATTATACCAAGCGCGATGATATAAGTATAGCCCGGATAGCATTCCA      48
GGAACGAGGCGCAGTCAACGGAACAACATTATTACAG                 37
AGATGAACGGTGTACAGAATGCCCCCTGC                         29
CTATTTCGGAACAGAGCCACCACCCTCATTTCTTTTTCATGAGGAA        46
CATGAAAGTATTAAACTGGTGCCACCCTCAGAGCCA                  36
TGCTCAGTACCAGGAGCGCAGAGGTTGAGGCAGGTC                  36
CGAGAGGGTAACAAAGTACAACGGAGATT                         29
TGTATCATCGCCTGAGTAAATTTAAGAACTGGCTCATT                38
CCAGAACGAGTATAAATTGTGTCGAAATCCGCGACCTGCTAAAGAATACA    50
TCAGTGAATAAGGCTTGGAAGAAAAATCTAC                       31
CATTACCCAAAACGGTCAATCATAAGGGAACCGAACTGACTAAAATACGT    50
ATCAAGAGTAATCTTGTGAGATTTAGGAATA                       31
AAACAGTTACCAGGCGCATCATAATCAAAATCACCG                  36
GTCAGTGCCTTGAGTAGAACCGCCTCCCTCAG                      32
TACAGGAGTGTGAGGCTGAGACTCCTCAAGAGAAGGATTAGTTTAGTACC    50
TTCCAGTAAGCGTCATCAGAACCACCACCAGAGC                    34
AGCCAGAATGGAACGGATAAGTGCCGT                           27
TTAATTTCAACACAAATAAATCCTCATTAA                        30
ATACCAGTCAGGTTTGCAAAAGAAGTTTTGCCAGAGAATCCCCCTCAAAT    50
GTTAATAAAACGAATACCAGACGACGATAAAAACCAATGACCATAAATC     49
GTAGAAAGATTCGAGGCATAGTAAGAGCAACACTAATAGTCAGAAGCA      48
CCACATTCAATGCCATCTTTTAGGCTGGCTGACCTTC                 37
GAACCAGAGCCACGTTTTCATCGGCATTTTCGGTCAACGCAGTATGTT      48
AGCCGCCACCCTCACAGAATCAAGTTTGCCTTTAGCGGTGGCAACATATA    50
CCACCCTCAGAGCCAATGAAACCATCGATAGCAGCACAAGTTTATTTTG     49
CGCCGCCAGCATAGTAGCACCATTACCATTAGCAAGTTACCAGCGCCA      48
AGACGATTGGCCTCTTGAGCCATTTGGGAATTAGAGTGAGGGAGGGAAGG    50
GTGAATTACCTTAATGTTTAGA                                22
TACATAACGAGGAAGCCCGAAAGACTTCACATGATTAAG               39
CTGGATAGCGGAATTATCACCGTCACCGATGATATTCACAATTTAATCATT   51
ATTCATTGGGGGTAATAGTAAATGCGA?TT?TGGGCTTGAGATGGT        46
GCTTTAAACAGGGCTTAGAGCTTAATTGCTGAATATAGTTGATTCCCAAT    50
AACGAGAAAATAGCGAGAGGCTACGTTGGCCCTGACGAGAAACA          44
AAAAATCAGGTCCTTTAATTGCTCCTTTTGATAAGAGATTAGATACATTT    50
TGACTATTTCATAACCCTCGTTCTAACGTAACAAAGCTGCTCAT          44
AAGCGGATTGCAGACCGGAAGCAAACTCCAACAGGTCTATTTTCATTTGG    50
AGATTAAGCCAAAAGGAATTACATCAGTACAAGAACCGGATATT          44
ACTCCTTATTTAGCCCCCTTATTAGCGTTCTAATGCAGA               39
AGCAAACGTAGAGAAACCGAGGAAACGCAATAATAACTGCTATTTTGCAC    50
TACATAAAGTCAGACTGTAGCGCCACCGACAGTGCCCGTAT             41
AAAGAAACGCATTAAGAAAAGTAAGCAGATAGCCGAACAACGCTAACGAG    50
CACGGAATCGTAATCAGTAGCGAGAACCAATAAGTTTTAACGGG          44
TCACAATCAATAAAACAATGAAATAGCAATAGCTATCACAAAATAAACAG    50
ATATGGTGCCGGAAACGTCACCGCCACACATGGCTTTTGATGA           43
AAGACAAAAGGGAACCCACAAGAATTGAGTTAAGCCCGAAACGATTTTTT    50
CAACCGATCCAGCAAAATCACCTGACAGGTCTCTGAATTTACCG          44
TAAATATTGACAACAAAGTCAGAGGGTAATTGAGCGCCTTTACAGAGAGA    50
CATTAAAGGTTCCAATACTGCGGAATCGTATGTTTTAAA               39
AAAGAACTGGAATATCGCGTTTTAATTCGACTACTAATAG              40
TATGCAACTAAGAATTAACTGAACACCCTGGGAAATTATT              40
TCTGCGAACGAGCACTCCAGCCAGCTTACGTTGTAAAACGACAATGAATC    50
CGCAAATGGTCAAAGTTTGAGGGGACGACCAGGTCGACTCTAGACCGCTTT   51
GGCGCGAGCTGAACGTTGGTGTAGATGCGTAATCATGGTCATTGAGTGA     49
TAGTAGCATTAGCCTTAAATCAAGATTAGTGGAATACCCA              40
CCAGCTACAATTTGTTTTAGCGAACCTCCTTCTGTCCAGACGACGGCATTT   51
CGTCTTTCCAGAAGATATAGAAGGCTTGCAGAACGCGCCTGTTCGCCAT     49
CCATATTATTTATTCATCGTAGGAATCACAAGAAAAATAATATGTATAAA    50
GTTTAACGTCAAAAAGTACCGCACTCATAACCAATCAATAATCAGCCTG?T   51
ATAACATAAAAATGGGAAGGGCGATCGGTGCGGCCAAGAAC             41
TGTCTGGAAGTTCCAGGCAAAGCGCCAGGCGATTAAGTTGGGTTGGGCG     49
GGGTATTAAACCAATGAAAATAGCAGCTAATATCAGAGAGATCGACATT     49
CTGCGCAACTGTCAGGGAAGCGCATTAGACGGGAAGTACGG             41
CTGGTGCCGGAAATCATTCCATATAACAATGCTGTAGCTCAACCATAAA?T   51
TCAGGAAGATCGTAGATTTAGTTTGACCGTCATTTTTGCGGATTTCAGAA    50
CGTGCATCTGCCTAACCTGTTTAGCTAAGGATTAGAGAGTACTTTACCC     49
AATGGGATAGGTCAAAGGTGGCATCAATTGCTTCAAAGCGAACCATCAAAA   51
GAGGTTTTGAATTCTCCGTGGGAACAAACGTCCGCTCACA              40
AGAACGCGAGGCTATCCTGAATCTTACCAAAGTTACCAGAAGAAATACA     49
TAGCAAGCAAATCGCCTAATTTGCCAGTTTTACCGAAGCCCTTTAAGACAC   51
GCCGTTTTTATTTCCCAATCCAAATAAAATAATAAGAGCAAGGAAAATTC    50
CCTTATCATTGCCTCTTCGCTATTACGCCAGCAACAGCTG              40
ATTCCACACAACCGACAAAAGGTAAAGTAACGACTTGCGG              40
ATTGCCCTTCAATCATAATTACTAGAAAAGGCTGTCTTT               39
CAGTGAGACGGGCTGGCGAAAGGGGGATGTGCTGCAATTCGCCATTCAGG    50
CCAGGGTGGTTTGCCCCAGCAGGAAGAAGCGCCGCTACAGGGCG          44
GCGGTTTGCGTATAACGCCAGGGTTTTCCCAGTCACGTCCGGCACCGCTT    50
GGCCAACTCCGAAATCGGCAAACGGGGTGACGAGCACGTATAA           43
GCCAGCTGCATTGGCCAGTGCCAAGCTTGCATGCCTGGACAGTATCGGCC    50
CCAGTCGGGCCCGAGATAGGGTCGGAACAATCAGAGCGGGAGCT          44
GCGCTCACTGCGGATCCCCGGGTACCGAGCTCGAATTGGCGCATCGTAAC    50
GCTAACTCAAGAGTCCACTATTGTTTTTAAAGGGATTTTAG             41
TGGGGTGCCTAAAGCTGTTTCCTGTGTGAAATTGTTAGCGGATTGACCGT    50
CGGAAGCATAAGGGCGAAAAACCGTCTATTATATAACTA               39
TCGAGCCAGCTGATGCAAATCCCTACCTATTACCTGAGCAAAAG          44
ATTTAGGCAGAGACAATAAACAACATGTTCAGCTAATATCCGGTATTCTA    50
ATTTAACAAAAACTTTTTCAAAAATAGTATCAAGAAAACAAAAT          44
CTTAATTGAGAATTATCAACAATAGATAAGTCCTGAATTACCGCGCCCAA    50
GCCAACGCCTGACCTAAATTTATAGCGATTTCATTTGAATTACC          44
AATTCTTACCACCCATCCTAATTTACGAGCATGTAGACGAGAACAAGCAA    50
TTAGTATCATAAATAAGGCGTTCTATT?AT?ACATAAATCAATATA        46
TAAACACCGGACCGCCTGGCCCTGAGAGACAAGTGTAGCGGAACCTTGCTT   51
TATGTAAATGTAATAAGAGAATATAAAGTACATACGAGC               39
CTGTAAATCGTCGAAATAAGAA                                22
GCTAGGGCGCTGGGTTGCAGCAAGCGGTCCACGCTGGTTTTTCTTTTCAC    50
GAGAAAGGAAGGGCGAAAATCCTGTTTGATGGTGGTGCGCGGGGAGAG      48
GATTTAGAGCTTGAATCCCTTATAAATCAAAAGAATAGAAACCTGTCGT     49
TAAAGCACTAAATTGAGTGTTGTTCCAGTTTGGAACACATTAATTGCGTT    50
CACCCAAATCAAAAAGAACGTGGACTCCAACGTCAAAAGTGTAAAGCC      48
TAGGTTGGGTCAGGGCGATGGCAAAATCGCGCAGAGG                 37
AGGTCTGAGAGAAATCGCAAGACAAAGAACGCGAGACGCCAACATGTA      48
CGCTGAGAAGAGTCTATATTTTAGTTAATTTCATCTTTCAACAGTAGGG     49
TCCTTGAAAACAATGGTTTGAAATACCGACCGTGTGATATGCGTTATACA    50
TGTGAGTGAATTCACGCTGCGCGTAACCAC                        30
CACACCCGCCGCGAACATCACTTGCCT                           27
CGTACTATGGTTGCTTAAAGCCGGCGAACGTGGC                    34
CGTGCTTTCCTAAAGAGTCTGTCCATCACGCAAATTAACCCGCCAGCCAT    50
AAACAGGAGGCCGATTTGGGGTCGAGGTGCCG                      32
ACAGGAACATACCAAGTTACCCACTACGTGAACCAT                  36
CGAATTATTCATTTCATTTTAACCTCCGGCT                       31
AAGATGATGAAATATACAGTAACAGTACCTTTTACATCGGCAAAGAAACC    50
TAATTACATTTAACAATAGCTTAGATTAAGA                       31
TTTTTTAATGGTTGCACGTAAAACAGAAATAAAGAAATTGGATGATGGCA    50
CATATCAAAATTATAAACAGATTAATTTTCCCTTAGAA                38
GAGTAGAAGATGGAAGGGTTAGAACCTAC                         29
CTTTGATTAGTAATCTTAATAGCGAAAGGAGCGGGC                36
GAGGCCACCGAGTACGTTAGCCTAAAGGGAGCCCCC                36
TCCTGAGAAGTGACGCTCAATCGTCTGAAATAAAGTTTGAGTAACA      46
TCGCCTGATTGCTTTGAGGTACGCCAGAA                       29
TTAACGTCAGATGAACAAACGAATTTATCAAAATCAT               37
GGATTATACTTCTGAATAAACTCAAACTATCGGCCTTGCCTTCTGACC    48
GAACAATATTACGTTGTAGCAATACTT                         27
TGCAACAGGAAAAAACCAGTAATAAAAGGGACATTCTGGCTGAATGGC    48
ACCTACATTTTGTTTTTATAATCAGT                          26
TTATCATTTTGCGGAAGAGAAACAATAACGGAT                   33
ACCAGAAGGAGCGGATTCGACAACTCGTATTAAATCCTTTGGCAAATCA   49
TTCCTGATTATCACGTAGATTTTCAGGT                        28
ATTCATCAATATAAAATACATTTGAGG                         27
TGAAAGCGTAAGATTAGAGCCGTCAATAGATTCCTGATTGTTT         43
CATTGGCAGAGATAGCCCTACAATCAATATC                     31
ATTTAGAAGTATTTTATCTAAAATATCTTGAAAAAT                36
ATATTTTCAACAGAGATAGAACCTGGTAATATCCA                 35
TATTAGTCAATACCGAACGAACCAAGAGGTGAGGCTTAAATTTAATGC    48
GCGAACTTTCACCAGTCACACGCGCTCATGGAAAT                 35
TGGTCAGTTGCCCGAACGTTATTAATTTTAGGATTATTTA            40
ACAGTTGATTGCTGAACCTCAAATACACCGCCTGTCACCAAGGAATT     47
GAGGAAGGAGACTTTACAAACAAATTATCATCATA                 35
GTATTAATCAAACCCTAAACATCGCCAGGTCA                    32
MODIFIED ORIGINALS

           5' CGACTATACGATAAC TTTTTT
           5'

KEY 1      5' GAACTGGAGTAGCACAGCATATCG TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT GTTATCGTATAGTCG (15


           5'
           5' GTATCGGCGTCGAA TTTTTT
           5'
           5'

KEY 2      5' GAACTGGAGTAGCACATGCCCGA TTTTTTTTTTTTTTTTTTTTTTTTT TTCGACGCCGATAC (10 nm poly
                                                                 Toehold
          ATTGCGTGCTTTCGAGGGGATTTTGCTAAGGA
          CTTTCCAGACTTTCAACAGTTTCAAGGAACAACTAAAACAACGTTAGT TTTTTT CGATATGCT

CACAGCATATCG TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT GTTATCGTATAGTCG (15 nm polyT for 6 nm gap)


          CTAAAGCACAACAGTGCCA
          CGCTGTAAAACCCAGCCACAGACA
          AACAACTAATAGAATACGTGGAGAAGAAGAGCCA                     TTTTTT TCGGGCAT
          GCAGCAAATTAGGAGCACT

CACATGCCCGA TTTTTTTTTTTTTTTTTTTTTTTTT TTCGACGCCGATAC (10 nm polyT for 4nm gap)
            Attached to Gold Strand   OTHER USEFUL STUFF
                                              U1C
            GTGCTACTCCAGTTC                5' GAACTGGAGTAGCAC

T for 6 nm gap)

                                      Coordinates on broken pieces from Sphere_StrandDisplacementHandle
                                      22[425] 23[438]
                                      23[385] 21[314]
            GTGCTACTCCAGTTC           21[286] 22[337]
                                      22[336] 21[285]
_StrandDisplacementHandles.json

				
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posted:3/23/2013
language:English
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