Docstoc

www.blackwellpublishing.comproductsjournalssupp

Document Sample
www.blackwellpublishing.comproductsjournalssupp Powered By Docstoc
					          Figure S1; ATase removal KG=1.0 mM

                                                    90

                                                    80

                                                    70
                                                              A.
                                                    60
                       v [mM min-1]




                                                    50

                                                    40
                                                                     vGS
                                                    30

                                                    20               vGOG

                                                    10               vGDH

                                                     0
                                                         -5    -4     -3    -2   -1       0        1   2   3         4    5

                                                                                      time [min]
                                                    12                                                                        600

                                                    10        B.                                                              500




                                                                                                                                     VAPPMAX,GS [mM min-1]
                    nAMP [GS AMP-1]




                                                     8                                                                        400
                                                               VAPPMAX,GS
                                                     6                                                                        300


                                                     4                                                                        200

                                                                    nAMP
                                                     2                                                                        100


                                                     0                                                                        0
                                                         -5    -4    -3     -2   -1      0     1       2   3         4    5

                                                                                  time [min]
                                                    80                                                                        2.0


                                                              C.
                       GLN [mM] & PIIKG1 [10-8 M]




                                                    60                                                                        1.5
                                                                                                                                    PIIUMP3KG3 [M]




                                                                PIIUMP3KG3
                                                                                                               GLN

                                                    40                                                                        1.0



                                                    20                                                                        0.5
                                                                                                                 PIIKG1


                                                     0                                                                        0.0
                                                         -5    -4     -3    -2   -1       0        1   2   3         4    5

                                                                                      time [min]



Transient response to a sudden increase in the ammonium availability from 0.05 to 1.0 mM and
removal of ATase at time zero. The a-ketoglutarate concentration was 1.0 mM. A: rates of glutamine
synthetase (vGS), glutamate synthase (vGOGAT) and glutamate dehydrogenase (vGDH). B: adenylylation
state of glutamine synthetase (nAMP) and the 'apparent' maximal rate of glutamine synthetase. C:
concentrations of glutamine (GLN), PII with one 2-ketoglutarate attached to it (PIIKG1), and PII
saturated with UMP and a-ketoglutarate (PIIUMP3KG3).
              Figure S2; UTase removal KG=1.0 mM


                                                        90

                                                        80

                                                        70
                                                                  A.
                                                        60
                           v [mM min-1]




                                                        50

                                                        40                  vGS

                                                        30
                                                                           vGOG
                                                        20
                                                                           vGDH
                                                        10

                                                         0
                                                             -5   -4         -3     -2    -1       0        1     2       3    4   5

                                                                                               time [min]
                                                        12                                                                             600

                                                        10
                                                                  B.                                                                   500




                                                                                                                                              VAPPMAX,GS [mM min-1]
                        nAMP [GS AMP-1]




                                                                           APP
                                                         8             V         MAX,GS                                                400


                                                         6                                                                             300


                                                         4                                                                             200

                                                                           nAMP
                                                         2                                                                             100


                                                         0                                                                             0
                                                             -5   -4        -3      -2    -1      0     1         2       3    4   5

                                                                                           time [min]
                                                        10                                                                             2.0


                                                                  C.
                           GLN [mM] & PIIKG1 [10-8 M]




                                                         8
                                                                                                                                       1.5
                                                                                                                                             PIIUMP3KG3 [M]




                                                                   PIIUMP3KG3
                                                         6

                                                                                                                GLN                    1.0
                                                         4

                                                                                                                                       0.5
                                                         2

                                                                                                                      PIIKG1
                                                         0                                                                             0.0
                                                             -5   -4         -3     -2    -1       0        1         2   3    4   5

                                                                                               time [min]




Transient response to a sudden increase in the ammonium availability from 0.05 to 1.0 mM and removal of
UTase at time zero. The a-ketoglutarate concentration was 1.0 mM. A: rates of glutamine synthetase (vGS),
glutamate synthase (vGOGAT) and glutamate dehydrogenase (vGDH). B: adenylylation state of glutamine
synthetase (nAMP) and the 'apparent' maximal rate of glutamine synthetase. C: concentrations of glutamine
(GLN), PII with one 2-ketoglutarate attached to it (PIIKG1), and PII saturated with UMP and a-ketoglutarate
(PIIUMP3KG3).
             Figure S3; PII removal KG=1.0 mM


                                        90

                                        80

                                        70
                                                  A.
                                        60
                        v [mM min-1]




                                        50

                                        40
                                                             vGS
                                        30

                                        20                   vGOG
                                        10                   vGDH

                                         0
                                             -5   -4         -3     -2   -1       0        1   2   3   4   5

                                                                              time [min]
                                        12                                                                     600

                                        10
                                                  B.                                                           500




                                                                                                                       VAPPMAX,GS [mM min-1]
                                                       APP
                     nAMP [GS AMP-1]




                                                   V         MAX,GS
                                         8                                                                     400


                                         6                                                                     300


                                         4                                                                     200


                                         2                                                                     100
                                                             nAMP

                                         0                                                                     0
                                             -5   -4         -3     -2   -1      0     1       2   3   4   5

                                                                          time [min]
                                        80                                                                     2.0
                                                                                                                     PIIUMP3KG3 [M] & PIIKG1 [10-8 M]




                                        60
                                                  C.                                                           1.5
                                                   PIIUMP3KG3
                             GLN [mM]




                                        40                                                                     1.0


                                                       PIIKG1
                                        20                                                                     0.5


                                                       GLN
                                         0                                                                     0.0
                                             -5   -4         -3     -2   -1       0        1   2   3   4   5

                                                                              time [min]




Transient response to a sudden increase in the ammonium availability from 0.05 to 1.0 mM and
removal of PII at time zero. The a-ketoglutarate concentration was 1.0 mM. A: rates of glutamine
synthetase (vGS), glutamate synthase (vGOGAT) and glutamate dehydrogenase (vGDH). B: adenylylation
state of glutamine synthetase (nAMP) and the 'apparent' maximal rate of glutamine synthetase. C:
concentrations of glutamine (GLN), PII with one a-ketoglutarate attached to it (PIIKG1), and PII
saturated with UMP and a-ketoglutarate (PIIUMP3KG3).
       Figure S4; transient regulatory contributions




The transient regulatory contributions of the regulators of GS are displayed upon a sudden increase
in the ammonium concentrations at time zero (Fig. 3). On the left the calculations were performed at
0.2 mM KG and on the right at 1.0 mM KG. The values for ADP have been divided by 10.
                          Figure S5; JN as function of the
                          maximal rates of GS and GOGAT




                      50



                          40
           J N [mM/min]




                          30



                           20

                                                                                             200
                                                                                            180
                           10                                                             160
                                                                                               in]



                                                                                         140
                                                                                                m




                                                                                       120
                                                                                             M/




                                0                                                     100
                                                                                         [m




                                    700                                             80
                                                                                         G




                                          600                                     60
                                                                                       GO




                                                500
                                                                                     V




                                                      400                       40
                                                            300
                                                 VG [         200             20
                                                   S mM/            100
                                                         min]             0




Ammonium assimilation flux as function the maximal rates of GS and GOGAT at conditions
of ammonium-limited chemostat (NH4+=0.05 mM, KG=0.2 mM, VGDH=360 mM/min). The
dot resembles the conditions of the kinetic model used in previous calculations (Table 3A; 3rd
row, 12th column).

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:0
posted:3/22/2013
language:Estonian
pages:5