MATERIALS AND METHODS
The present study was carried out at Giza research station,
Agricultural Research Center, Egypt during three winter seasons of
2002/03, 2003/04, and 2004/05.
Genetic materials and field processes:
The materials used in this study included five F3-lentil
populations, drived from crosses made among nine parents (Table 1).
The crosses were made at the International Center for Agricultural
Research in the Dry Areas (ICARDA) in 2000/01 season, then F1 and
F2 were sown as bulks in 2001/02 season (including summer off-
season). In 2002/03, about 6000 seeds from each F3 population were
sown on 18 December 2002, on 40 rows, 3-m long and 0.3 m width,
with intra-row spacing of 2 cm (150 plants/m2). The wide spread local
variety Giza 9 and the early maturing variety Sinai 1(Hamdi et al.,
2002) were used as checks, and planted in the same areas and density
as F3 populations.
Table (1). Cross number, pedigree and origin of the parents for the five
Cross Pedigree Origin
1 ILL 7537 x ILL 4404 ICARDA
2 ILL 7617 x ILL 4147 ICARDA
3 ILL 7021 x ILL 2580 ICARDA
4 ILL 6260 x 89510 ICARDA
5 ILL 6467 x ILL 4147 ICARDA
ILL = International Legume Lentil.
No seed inoculation was made, and nodulation was adequate
throughout. Fertilizer at the rate of 15 kg N/feddan and 30 kg P2
O5/feddan were added to the soil prior to planting. All recommended
field practices were applied to the soil prior to planting. The herbicide
Gessagard was sprayed at pre-emergence at the rate of 1 kg
product/feddan, to control broad-leafed weeds. Thereafter, hand
weeding was undertaken as necessary. Three irrigations (in addition to
sowing irrigation) were given at 30, 60, and 90 days from sowing.
At maturity, 100 plants from each F3 population were selected on
the basis of number of pods/plant, which is the major yield – related
lentil character. Each selected plant was harvested individually and
saved in a paper bag. A total of 20 plants were selected separately and
randomly from each F3 population, as well as, from check varieties,
and the following characters were measured for each plant:
1- Plant height (cm).
2- Total number of branches/plant.
3- Number of pods/plant.
4- Number of seeds/plant.
5- Number of seeds/pod (estimated from 20 pods/F3
6- Biological yield /plant (g).
7- Seed yield/plant (g).
8- Harvest index (seed yield/plant÷ biological yield
9- Plant growth vigor (1-high, 2- mediam, 3-low vigor).
10- Days to 50% flowering.
11- Days to 90% maturity.
The characters numbers 9, 10 and 11 were measured on the plot-
The 100 selected plants from each F3 Population were ranked
according to their seed yield. The plants, significantly exceeded the
yield of Giza 9, were selected to be grown as F4 families in the next
season. The total number of F3 selected plants F3 to be grown as F4
families were 6, 6, 5, 17, and 21 for cross 1, 2, 3, 4 and 5,
respectively. The seeds of these plants were saved for planting F4
Planting of F4 families:
In 2003/04-winter season, the seeds of the selected F3 plants were
planted as F4 families. The seeds of each F4 family were sown in a
single row, 1-m long and 0.3 m width with 150 seeds/m2. The planting
was done on 13 December 2003. The numbers of F4 families were 6,
6, 5, 17 and 21 for cross 1, 2, 3, 4 and 5, respectively (as mentioned
above). Each of Giza 9 and Sinai 1 were also grown on 1-m long
single rows as in F4 families. A randomized complete block design
with two replicates was used. Each replicate contained 57 plots (55
plots for all the families and two plots for Giza 9 and Sinai 1). Field
practices were applied as used in F3 generation. The characters: plant
growth vigor (1-3), days to 50% flowering and days to 90% maturity
were measured on the plot basis for every F4 family and the check
varieties Giza 9 and Sinai. At harvesting, each plant in every F4 family
in every plot was harvested separately, and all the characters measured
in F3 generation were also measured on plant basis. The highest three
seed- yielding plants in every F4 family were selected and saved to be
grown in F5 generation. The remaining plants of each family were
bulked together and saved.
Planting of F5 families:
In 2004/05-winter season, the seeds of the three selected F4 plants
plants were planted as F5 families. F5 families were sown on single
rows, 1-m long and 0.3 m width with 150 seeds/m2. The planting was
done on 27 October 2004. The numbers of F5 families were 18, 18, 15,
51 and 63 for cross 1, 2, 3, 4 and 5, respectively. Giza 9 and Sinai 1
were also grown in 1-m long single rows as in F4 families. A
randomized complete block design with three replicates was used.
Each replicate contained 167 plots (165 plots for all the five crosses
and two plots for Giza 9 and Sinai 1). Field practices were applied as
used in F4 generation. The characters: plant vigor (1-3), days to 50%
flowering and days to 90% maturity were measured on the plot basis
for every F5 family and the check varieties Giza 9 and Sinai 1. At
harvesting, each plant in every F5 family in every plot was harvested
separately, and all the characters measured in F4 generation were also
measured on plant basis.