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fagr_SALWA . HASSANEIN_4200017

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fagr_SALWA . HASSANEIN_4200017 Powered By Docstoc
					            MATERIALS AND METHODS

    The present study was carried out at Giza research station,
Agricultural Research Center, Egypt during three winter seasons of
2002/03, 2003/04, and 2004/05.

Genetic materials and field processes:
     The materials used in this study included five F3-lentil
populations, drived from crosses made among nine parents (Table 1).
The crosses were made at the International Center for Agricultural
Research in the Dry Areas (ICARDA) in 2000/01 season, then F1 and
F2 were sown as bulks in 2001/02 season (including summer off-
season). In 2002/03, about 6000 seeds from each F3 population were
sown on 18 December 2002, on 40 rows, 3-m long and 0.3 m width,
with intra-row spacing of 2 cm (150 plants/m2). The wide spread local
variety Giza 9 and the early maturing variety Sinai 1(Hamdi et al.,
2002) were used as checks, and planted in the same areas and density
as F3 populations.
         Table (1). Cross number, pedigree and origin of the parents for the five
                    crosses used.

           Cross           Pedigree                  Origin
            No.
           1        ILL 7537 x ILL 4404       ICARDA
           2        ILL 7617 x ILL 4147       ICARDA
           3        ILL 7021 x ILL 2580       ICARDA
           4        ILL 6260 x 89510          ICARDA
           5        ILL 6467 x ILL 4147       ICARDA

         ILL = International Legume Lentil.

    No seed inoculation was made, and nodulation was adequate
throughout. Fertilizer at the rate of 15 kg N/feddan and 30 kg P2



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 O5/feddan were added to the soil prior to planting. All recommended
 field practices were applied to the soil prior to planting. The herbicide
 Gessagard was sprayed at pre-emergence at the rate of 1 kg
 product/feddan, to control broad-leafed weeds. Thereafter, hand
 weeding was undertaken as necessary. Three irrigations (in addition to
 sowing irrigation) were given at 30, 60, and 90 days from sowing.

    At maturity, 100 plants from each F3 population were selected on
 the basis of number of pods/plant, which is the major yield – related
 lentil character. Each selected plant was harvested individually and
 saved in a paper bag. A total of 20 plants were selected separately and
 randomly from each F3 population, as well as, from check varieties,
 and the following characters were measured for each plant:
     1- Plant height (cm).
     2- Total number of branches/plant.
     3- Number of pods/plant.
     4- Number of seeds/plant.
     5- Number of seeds/pod (estimated from 20 pods/F3
         population).
     6- Biological yield /plant (g).
     7- Seed yield/plant (g).
     8- Harvest index (seed yield/plant÷ biological yield
         /plant)× 100).
     9- Plant growth vigor (1-high, 2- mediam, 3-low vigor).
     10- Days to 50% flowering.
     11- Days to 90% maturity.
     The characters numbers 9, 10 and 11 were measured on the plot-
 basis.
     The 100 selected plants from each F3 Population were ranked
 according to their seed yield. The plants, significantly exceeded the
 yield of Giza 9, were selected to be grown as F4 families in the next
 season. The total number of F3 selected plants F3 to be grown as F4
 families were 6, 6, 5, 17, and 21 for cross 1, 2, 3, 4 and 5,
 respectively. The seeds of these plants were saved for planting F4
 generation.
Planting of F4 families:


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     In 2003/04-winter season, the seeds of the selected F3 plants were
 planted as F4 families. The seeds of each F4 family were sown in a
 single row, 1-m long and 0.3 m width with 150 seeds/m2. The planting
 was done on 13 December 2003. The numbers of F4 families were 6,
 6, 5, 17 and 21 for cross 1, 2, 3, 4 and 5, respectively (as mentioned
 above). Each of Giza 9 and Sinai 1 were also grown on 1-m long
 single rows as in F4 families. A randomized complete block design
 with two replicates was used. Each replicate contained 57 plots (55
 plots for all the families and two plots for Giza 9 and Sinai 1). Field
 practices were applied as used in F3 generation. The characters: plant
 growth vigor (1-3), days to 50% flowering and days to 90% maturity
 were measured on the plot basis for every F4 family and the check
 varieties Giza 9 and Sinai. At harvesting, each plant in every F4 family
 in every plot was harvested separately, and all the characters measured
 in F3 generation were also measured on plant basis. The highest three
 seed- yielding plants in every F4 family were selected and saved to be
 grown in F5 generation. The remaining plants of each family were
 bulked together and saved.

Planting of F5 families:
    In 2004/05-winter season, the seeds of the three selected F4 plants
 plants were planted as F5 families. F5 families were sown on single
 rows, 1-m long and 0.3 m width with 150 seeds/m2. The planting was
 done on 27 October 2004. The numbers of F5 families were 18, 18, 15,
 51 and 63 for cross 1, 2, 3, 4 and 5, respectively. Giza 9 and Sinai 1
 were also grown in 1-m long single rows as in F4 families. A
 randomized complete block design with three replicates was used.
 Each replicate contained 167 plots (165 plots for all the five crosses
 and two plots for Giza 9 and Sinai 1). Field practices were applied as
 used in F4 generation. The characters: plant vigor (1-3), days to 50%
 flowering and days to 90% maturity were measured on the plot basis
 for every F5 family and the check varieties Giza 9 and Sinai 1. At
 harvesting, each plant in every F5 family in every plot was harvested
 separately, and all the characters measured in F4 generation were also
 measured on plant basis.




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