Titre du project USING MICROFLUIDICS TO MANIPULATE AND
ANALYZE CELL DIVISION TIMING IN C. ELEGANS
Laboratoires ISREC - Laboratory of Pierre Gönczy
STI – Philippe Renaud
Professeurs Pierre Gönczy
phone : 32596, 36797
Laboratories of Pierre Gönczy (FSV) and Philippe Renaud (STI).
The early C. elegans embryo allows for an analysis of cell division processes with
high spatial and temporal resolution in live specimens. In the two-cell stage embryo,
one of the two cells divides before the other one. Although this time difference is
invariable and under the control of evolutionarily conserved polarity proteins,
whether it is important for development is not known.
During this masters project, this question will be addressed by generating cell- chip
devices using microfluidics and microelectrodes, with the aim of subjecting the two
cells of a wild- type C. elegans embryo to different temperatures. Given that cell
cycle progression is temperature-dependent, this is expected to result in a
synchronous cell division, whose consequences will be analyzed using live imaging
and immunofluorescence analysis of fixed specimens.
SSV – 2009/2010