Bioconversion of phospholipids in supercritical carbon dioxide media T. Shiina1, K. Onoyama1,Y. Inoue2, and K. Takahashi*1, 1Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan, 2Lipid Lab., Kawasaki, Japan.. Phospholipase A1 from Aspergillus oryzae (SANKYO LIFETECH CO., LTD., Tokyo) mediated selective partial hydrolysis in supercritical carbon dioxide media using squid meal phosphatidylcholine (PC), a typical marine phospholipid rich in DHA exclusively in sn-2 position of the glycerol backbone as substrate, was practically viable to enrich DHA more. The principle of this reaction was cleaving off fatty acid moiety in sn-1 position leaving the DHA moiety in the sn-2 position of the glycerophospho-backbone. Another phospholipid bioconversion trial done in supercritical carbon dioxide media was the transphosphatidylation of squid meal (PC) to phosphatidylserine (PS) which is usually denoted as “DHA-PS”, a typical brain health beneficial compound. This reaction was carried out by Phospholipase D from Streptomyces sp. (ASAHI KASEI CO., LTD., Shizuoka, Japan). Expectedly, the desired transphosphatidylation proceeded in the supercritical carbon dioxide media though both phospholipids are basically insoluble in the media. Bioconversion of lipids in supercritical carbon dioxide media must be one of the most highly potential alternative solvent free systems in the future.