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Biotechnology Restriction Enzymes and Plasmids - Michigan

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					             Michigan Department of Education
             Technology-Enhanced Lesson Plan 2007
Title:   Biotechnology
Created by:     Christine G. Harris

Lesson Abstract:      -Students will Research the Human Genome project and understand the
importance of mapping the human genome. Students will interpret pedigree, electrophoresis gel plates
and DNA fingerprints. Students will construct a recombinant DNA model. Students will
debate/discuss bioethical issues and current topics in genetics.

Subject Area: Biology
Grade Level: 10th
Unit Title: Recombinant DNA Technology


Michigan Educational Technology Standards Connections:

Michigan Educational Technology Standards (METS) - 9th to 12th Checklist
      1. Basic Operations and Concepts
              a. Students demonstrate a sound understanding of the nature and operation of
                 technology systems.
      1. Students discuss emerging technology resources (e.g., podcasting, webcasting, compressed
          video delivery, online file sharing, graphing calculators, global positioning software).
      3. Students understand the importance of both the predictable and unpredictable impacts of
          technology.
      6. Students understand that access to online learning increases educational and workplace
          opportunities

Michigan High School Content Expectations Connections:

    B4.2 DNA, RNA, and Protein Synthesis

    B4.2h – Recognize that genetic engineering techniques provide great potential and
           responsibilities.

    B4.r2i – Explain how recombinant DNA technology allows scientists to analyze the
            structure and function of genes. (recommended)

    B4.r5x Recombinant DNA
            Recombinant DNA technology allows scientist in the laboratory to combine the genes
            from different sources, sometimes different species, into a single DNA molecule. This
            manipulation of genes using bacterial plasmids has been used for many practical
            purposes including the mass production of chemicals and drugs. (recommended)



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    B4.r5a – Explain how recombinant DNA technology allows scientists to analyze the structure and
             function of genes. (recommended)
    B4.r5b – Evaluate the advantages and disadvantages of human manipulation of DNA.
             (recommended)


Estimated time required to complete lesson:
Estimated total time: 225 minutes
Daily estimate: 45 minutes
Number of days: 5

Note: the lesson should take a minimum of 200 minutes.

Instructional resources:
http://www.ornl.gov/sci/techsources/HumanGenome/project/info.shtml
http://www.genome.bov/11006943
http://www.genome.gove/HGP/
http://genome.gov/12011251
http://www.accessexcellence.org/RC/AB/IE/
www.encarta.msn.com keyword: plasmids
www.infoplease.com keyword: plasmids


Prior required technology skills:
      -Students need to be familiar with the use of their assigned laptops
      -Students will be given websites to visit
      -Students will need to be able to use the following programs:
            -Microsoft word
            -Microsoft excel
            -Microsoft paint

Sequence of Activities:
      Day One and Day Two:
      1. Introduction to biotechnology Notes/lecture using PowerPoint
      2. Class discussion on the Human Genome Project
      3. Human Genome Activity
            a. Lap tops and or a computer lab will be necessary
            b. This is to be done individually
            c. What is not completed in class must be completed as homework

      Day Three:
      1. Turn in Human Genome Activity
      2. Classroom Discussion about the Human Genome Project:
            a. Advantages to this project
            b. Future advancements
            c. Technology involved
      4. Notes/Lecture: Biotechnology and restriction enzymes

      Day Four:
      1. Lab: Paper Plasmids
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      Day Five:
      1. Review HGP and Lab
      2. Quiz: Restriction Enzymes and Plasmids
      3. Begin Lecture Notes on Gel Electrophoresis


Assessments:
              Human Genome Project – understanding the meaning
              Lab: Paper Plasmids and Restriction Enzymes

Pre-Assessment:
    1.   What is the Human Genome project?
    2.   What is biotechnology?
    3.   What is the purpose of biotechnology?
    4.   Are the moral and ethical issues surrounding biotechnology?
    5.   What is a restriction enzyme?
    6.   What is a plasmid?
    7.   What role do plasmids and restriction enzymes play in biotechnology?

      Scoring Criteria: Briefly explain how the pre-assessment would be scored (actual
      test? teacher observation?) Include an answer key, if necessary.

Post-Assessment:
      Topic Quiz: Restriction Enzymes and Plasmids
      Unit Test: This would be given at the end of the unit (includes answer key)
                  Unit Test Answer Sheet


      Scoring Criteria: Test answer key include in the document.

Technology (hardware/software):
     -Computer (lap top, desk Mac or PC)
      -Microsoft word
      -Microsoft excel
      -Microsoft paint

Key Vocabulary:
      Plasmids
      Recombinant DNA
      Bacteria
      Genome
      Genes
      Genetic disorders
      Restriction enzymes
      Digest
      Ligase
      Antibiotic
      Resistance
      Media (agar growth plates)
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Application Beyond School:
      -Work World
           Genetic Counseling
           Lab Research
           Medical advances




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UDL Connections:
          -Notes and lecture will be presented via the following:

All presentations will be presented using a LCD projector which allows large
print and color to be displayed. This allows all students to see the material
equally.
                  *Power point presentation
                        -including pictures and animations

                       *Elmo: modified overhead projector which allows for teacher
                             to write as material is presented.

                               -The material can be displayed using different colors.

                               -Material can be presented in a very large font this way
                                as well.

                       *Human Genome Project:
                            -web site information can be set up in the text reader
                       *The Paper Plasmid Lab:
                             -can be set up in the text reader. This will assist
                              students in the directions of the lab


Teacher Reflection and Notes:

This lesson takes a relatively difficult concept to grasp and provides a number of useful ways for
students to get their hands on it. The use of computers for both information disseminating and
gathering is used. A paper, scissor lab is used to allow students the time to cut, paste, think and see
what is happening.




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