Ochratoxin A in Oil Seeds and Nuts – Seldom Present – Investigations from 2012

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Ochratoxin A in Oil Seeds and Nuts – Seldom Present
– Investigations from 2012

Report from a day in the lab

In 2012 oil seeds (poppy, sunflower, pumpkin, and flax seeds) as well as
pistachios and pine nuts taken from the administrative districts of Stuttgart
and Karlsruhe were analyzed at CVUA Stuttgart for the presence of
Ochratoxin A (OTA). A total of 89 samples were analyzed.

          There are no maximum limits established at the EU or national
          level for Ochratoxin A in oil seeds and nuts. For reference, howev-
          er, the EU-wide maximum limit (ML) set by regulation 1881/2006
          for dried wine grapes is 10 µg/kg and for cereals and cereal flour, 3
          No Ochratoxin A was detected in the 14 analyzed poppy seed and
          pine nut samples.
          Most of the pistachio samples and sunflower seeds were also free
          of Ochratoxin A (46 of 50 and 11 of 14, respectively).
          The highest quantities of Ochratoxin A were found in a sample of
          sunflower seeds, with 20.6 µg/kg and in pistachios, with 12.2

These findings show that there is no reason for concern regarding the
residue situation.

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Ochratoxin A is a mycotoxin that is formed from various species of genus
penicillium und aspergillus, which are found throughout the world in na-
ture. When plant-based foods are stored under inadequate conditions, in
contrast to the aflatoxin species, toxins can develop, even in moderate
climate zones. OTA occurs almost exclusively in plant-based foods such
as cereals, legumes, coffee, beer, wine grapes and their products (e.g.
dried wine grapes, red grape juice, and red wine), cocoa, nuts and spices.

The long half-life of OTA in animal and human organisms is problematic.
After the consumption of contaminated products the process of eliminating
the toxins from the body is very slow. According to present knowledge,
OTA is considered to be especially damaging to the kidneys and is immu-
nosuppressive. Animal studies have shown OTA to have carcinogenic
and teratogenic effects. The International Agency for Research on Cancer
(IARC) has categorized OTA as a probable carcinogen for humans.

Following reports on Ochratoxin A findings in pistachios from California,
among other regions, all samples of pistachios, oil seeds and nuts that
were to be analyzed for aflatoxin at CVUA Stuttgart in 2012 were also in-
vestigated for Ochratoxin A.
A preview of these investigations can be found in the article „Mykotoxine
in              Pistazien“     (Mycotoxins           in         Pistachios)
Pdf=No) from 22 June, 2012.

Sunflower and pumpkin seeds, as well as pistachios, are favorites for nib-
bling and eating as snacks. Especially in East Europe, sunflower seeds
are often eaten as snacks on the go. They are also used in the manufac-
turing of sunflower oil. Poppy seeds, on the other hand, are mostly used
in pastries and baked goods. Pumpkin seeds are versatile, in that they can
be eaten as snacks, made into pumpkinseed oil, and also be used in
baked goods.

Pistachios grow on evergreen deciduous trees that can live for hundreds
of years, and are botanically categorized as stone fruit. Because female
and male blossoms grow on different trees, pollination and its ensuing fruit
can only occur when both male and female trees are on the same planta-
tion, or when branches from the male tree are grafted onto a female tree.
Pistachios thrive in dry, desert-like areas. The biggest pistachio farms are
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in Iran, the USA and Turkey. Pistachios usually come roasted and salted,
as munchies.         They taste a bit sweet, almondesque, and full-flavored.
Green, unroasted pistachios are used in the production of confectionaries
(Mozart chocolates, pralines, or baklava), ice cream, and in sausage
products. Pine nuts are used in Mediterranean, Turkish, and Arabic kitch-
ens for baking, in rice dishes, or just to snack on.

Because the seeds of Mediterranean pine trees are very expensive, seeds
of the Korean pine tree are often imported from Korea, China, and Paki-
stan. These Asian pine nuts are easy to recognize for their triangular
shape and dark tips. In comparison to the South European seeds, they are
less aromatic and contain more fat. They are described as having a bitter,
metallic taste and are often reported as causing a serious but temporary
taste impairment, which can last from a few days to several weeks.

Opium poppy seeds contain a small amount of opiates. In Germany there
are just two types with small quantities of morphine that are permitted to
be grown, while in Austria it has long been a tradition to grow opium poppy
seeds, and many pastry recipes contain them. Due to high consumer de-
mand for baked goods in Germany, local stores offer poppy seeds import-
ed from other countries, whose morphine content can be very high. The
opiates can be rendered harmless, however, during the baking process.
(„Mohn          im    Haushalt    richtig   verwenden    -    aber    wie?")
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The following table shows the results for the analyses of Ochratoxin A in
the samples.

Table 1
Ochratoxin A found in various oil seed and nut products.
                           No.        No. Samples       Average    Highest
                        Samples       with Content (Samples with   Quantity
Ochratoxin A
                                         > LoQ*        Residues)   Detected
                                                         [µg/kg]    [µg/kg]
Roasted Pistachios         45           3 (6.7%)           4.8       12.2
Green Pistachios            5           1 (20%)           0.24       0.24
Sunflower seeds            14          3 (21.4%)           8.9       20.6
Pumpkin seeds               3          2 (66.7%)           3.4        6.0
Pine nuts                  10               0               -          -
Poppy seeds                 4               0               -          -
Others (4 Trail Mix-
es, 2 Peanut prod-
                            8          3 (37.5%)          0.34       0.38
ucts, 1 Flaxseed, 1
*LoQ Limit of quantification Ochratoxin A: 0,4 µg/kg.

The results show that OTA contamination of the analyzed products was
fairly limited. Only 8 % of all 50 pistachio samples contained OTA. Sun-
flower seeds were found to have the highest number of samples (21.4%)
above the limit of quantification. In contrast, none of the 10 pine nut or 4
poppy seed samples contained OTA. Because only 3 samples of pumpkin
seeds were analyzed, it is not possible to make a representative state-
ment regarding contamination with OTA.

The pistachio samples with the highest level of OTA contamination (12.2
µg/kg) came from Turkey. The same sample was also found to contain
aflatoxin B1 und total aflatoxin (with 6.1 µg/kg and 6.7 µg/kg, respectively);
however, the quantity was under the legal levels stipulated in EU regula-
tion 1881/2006 (6 µg/kg and 10 µg/kg, respectively, taking into account
recovery and measurement uncertainty).
The pumpkin seeds with 6.0 µg/kg Ochratoxin A originated in China; the
sunflower seeds with 20.6 µg/kg came from the Ukraine. In neither of the-
se samples was aflatoxine detected.

So far there has been no maximum limit established for OTA in oil seeds
and nuts at the EU or national level. However, according to the official
statement from 26 Sept., 2012 regarding the toxicological evaluation of
Ochratoxin A in nuts and oil seeds, the National Institute for Risk Evalua-
tion (BfR) does not expect any negative health effects due to the con-
sumption of pistachios containing 12.2 µg/kg of Ochratoxin A or of sun-
flower seeds containing 20.6 µg/kg of Ochratoxin A.
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[1]      Wikipedia Online-Lexicon.

[2]      IARC 1993: International Agency for research on Cancer, 1993.
         Ochratoxin A (Group 2B). Summaries and Evaluations 56: 489.
         Lyon, France.
         ochra.html [19.09.2012].

[3]      Toxikologische Bewertung des Bundesinstituts für Risikobewer-
         tung BfR von Rückständen an Ochratoxin A in Schalenobst und
         Ölsaaten vom 26.09.2012 (Toxicological Evaluation of Ochratoxin
         A Residues in Nuts and Oil Seeds by the National Institute of Risk
         Evaluation (BfR), 26 Sept., 2012.).

Photo credits:
Pistazien, Sara Hegewald,, Image-ID= 586731.
Kürbiskernknäcke, w.r.wagner,, Image-ID= 214046.

Dr. Renate Schnaufer.

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Description: In 2012 oil seeds (poppy, sunflower, pumpkin, and flax seeds) as well as pistachios and pine nuts taken from the administrative districts of Stuttgart and Karlsruhe were analyzed at CVUA Stuttgart for the presence of Ochratoxin A (OTA). A total of 89 samples were analyzed.