Thin Layer Chromatography TLC Analysis of Commercial Analgesics by FR3n75


									Name:                                                                              Experiment Report

         Thin Layer Chromatography (TLC) Analysis of
                    Commercial Analgesics
                     (Describe your conclusions, observations, and identify your
                     unknowns. Use complete sentences and proper grammar.)

Attach TLC Plate here

Standards Plate
                        Acetaminophen      Aspirin      Caffeine      Ibuprofen       Solvent Front
 Distance Traveled

Unknowns Plate Data                                                 Unknown Number:
                                 Unknown                              Solvent Front
 Distance Traveled

Identify Your Unknown
Trade Name of Your Unknown

1.   What factors affect Rf values in TLC? List at least four factors.

2.   You ran the unknown tablet against each known even though you had already
     determined the Rf values of the knows. Why?

3.   If the known caffeine solution were made by dissolving caffeine in
     dichloromethane (CH2Cl2 ) rather than in methanol, would this change affect the Rf
     for the known caffeine? Explain.

4.   In carrying out an analysis of a mixture, what do you expect to see when the TLC
     plate has been allowed to remain in the developing chamber too long, so that the
     solvent has reached the top of the plate?

5.   What will be the result of applying too much compound to a TLC plate?

6.   Why is it necessary to run TLC in a closed container and to have the interior vapor
     saturated with the solvent?

7.   How could TLC be used for a preparative separation? That is, how could you use
     TLC to isolate compounds from a mixture so that the individual pure compounds
     could be studied further

8.   Why would it not be wise to use a pen when drawing lines on the TLC plates?


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