Enabling Marker-Assisted Breeding in Rosaceae - RosBREED by linxiaoqin

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									       Enabling Marker-Assisted Breeding in Rosaceae
                                                                          Project Director
Dedicated to the genetic
 improvement of U.S.                                             Amy Iezzoni
                                                                 A I        i
                                                                 Michigan State University
   rosaceous crops                                               iezzoni@msu.edu
  www.rosbreed.org                                               517.355.5191 x 1391


                                         Team Leaders
                                           Breeding
                                         James Luby
                                     University of Minnesota
                                      lubyx001@umn.edu
Socio‐Economics                                   Marker‐Assisted Breeding Pipeline
Chengyan Yue                                      Cameron Peace
  University of Minnesota                           Washington State University
  yuechy@umn.edu                                    cpeace@wsu.edu
Breeding Information Management System            Genomics
Gennaro Fazio                                     Dorrie Main
  USDA‐ARS Plant Genetic Resources Unit                   g                  y
                                                   Washington State University
  gf35@cornell.edu                                 dorrie@wsu.edu
Stakeholder                                       Pedigree‐Based Analysis 
Jim McFerson                                      Eric van de Weg
  Washington Tree Fruit Research Commission          Plant Research International – Netherlands              
  mcferson@treefruitresearch.com                     eric.vandeweg@wur.nl 
Extension                                         Genotyping
Cholani Weebaddee                                 Nahla Bassil
  Michigan State University                         USDA‐ARS National Clonal Germplasm
  weebadde@msu.edu                                  nahla.bassil@ars.usda.gov


                                    What is RosBREED?
                                    What is RosBREED?
 RosBREED is a multi‐state, multi‐institution project dedicated to genetic improvement of U.S. 
rosaceous crops by targeted applications of genomics knowledge and tools to accelerate and 
increase the efficiency of breeding programs.  This Coordinated Agricultural Project is funded 
through the Specialty Crops Research Initiative by a combination of federal and matching funds. 

                                                                                                                1
                                    RosBREED Team Members
Marco Bink                                     Tom Gradziel
Plant Research International, Netherlands      University of California ‐ Davis
marco.bink@wur.nl                              tmgradziel@ucdavis.edu
Susan Brown
Susan Brown                                    James Hancock 
                                               James Hancock
Cornell Univ.                                  Michigan State University
skb3@cornell.edu                               hancock@msu.edu
David Byrne                                    Raymond Jussaume
Texas A&M                                      Washington State University
d‐byrne@tamu.edu                               rajussaume@wsu.edu
John Clark                                     Vicki McCracken
University of Arkansas                         Washington State University
jrclark@uark.edu                               mccracke@wsu.edu
Carlos Crisosto                                Nnadozie Oraguzie
Univ. of California – Davis                    Washington State University
carlos@uckac edu
carlos@uckac.edu                               noraguzie@wsu.edu
                                               noraguzie@wsu edu
Thomas Davis                                   Gregory Reighard
Univ. of New Hampshire                         Clemson University
tom.davis@unh.edu                              grghrd@clemson.edu
Kate Evans                                     Alexandra Stone 
       g                  y
Washington State University                        g                 y
                                               Oregon State University
kate_evans@wsu.edu                             stonea@hort.oregonstate.edu
Chad Finn                                      Mykel Taylor
USDA‐ARS , Corvallis, OR                       Washington State University
finnc@science.oregonstate.edu                  m_taylor@wsu.edu
Karina Gallardo                                Dechun Wang
Washington State University                    Michigan State University
karina_gallardo@wsu.edu                        wangdech@msu.edu
Ksenija Gasic                                  Kenong Xu
Clemson University                             Cornell University
kgasic@clemson.edu                             kx27@cornell.edu

                                RosBREED Project Associates
Riaz Ahmad (U. of Calif. – Davis)              Steven McKay (U. of Minn.)
rnuahmad@ucdavis.edu                           mckay098@umn.edu
Matt Clark (U. of Minn.)                       John McQueen (Oregon State Univ.)
clark776@umn.edu                               mcqueenjpg@gmail.com
Daniel Edge‐Garza (Wash. State Univ.)          Paul Sandefur (U. of Arkansas)
esteboone@wsu.edu                              psandefu@uark.edu
Lise Mahoney (U. of New Hampshire)             Travis Stegmeir (Michigan State Univ.)
lisem@unh.edu                                  stegmeir@msu.edu


                                                                                        2
                      International Partners and Nature of Partnership

Sequencing/Molecular Groups 
    David Chagné ‐ Plant & Food Research Palmerston North, 4474, New Zealand 
    Lee Meisel ‐ Center of Plant Biotechnology, Ecology & Natural Resources. Andres Bello Univ 
       Santiago, Chile 
    Jasper Reese ‐Dept of Biotechnology, Univ of the Western Cape, Bellville, 7535, South Africa 
    Dan Sargent ‐ Department of Plant Breeding and Genetics, East Malling Research, East 
       Malling, UK. 
    Herman Silva ‐ Center of Plant Biotechnology, Ecology & Natural Resources. Andres Bello Univ 
       Santiago, Chile 
 
INRA – Breeding & Genetics teams 
    Beatrice Denoyes‐Rothan ‐ Unité de Recherche des Espèces Fruitières (UREF), INRA CR 
       Bordeaux.Villenave d'Ornon, France 
    Elisabeth Dirlewanger ‐ Unité de Recherche des Espèces Fruitières (UREF), INRA CR 
       Bordeaux.Villenave d'Ornon, France 
    Charles‐Eric Durel‐ Unité de Génétique et Horticulture (GENHORT), INRA CR Angers, 
       Beaucouze, France 
    Francois Laurens ‐ Unité de Génétique et Horticulture (GENHORT), INRA CR Angers, 
       Beaucouze, France 
    Jose Quero Garcia ‐ Unité de Recherche des Espèces Fruitières (UREF), INRA CR 
       Bordeaux.Villenave d'Ornon, France 
    Benedicte Quilot‐Turion ‐ Unité de Génétique et Amélioration des Fruits et Légumes (UGAFL), 
       INRA CR Avignon, France 




                          Collaborators and Nature of Collaboration

Pedigree Based Analysis ‐ Plant Research International, Netherlands  
    Hans Jansen ‐ Institute for Hort Plant Breeding 
    Chris Maliepaard ‐ Plant Breeding 
    Roeland Voorrips ‐Centre for Plant Breeding & Reproduction Research.   
        
Sequencing/Molecular Groups 
    Amit Dhingra ‐ Dept of Hort. & Landscape Arch., Washington State Univ., Pullman, WA  99164
    Bryon Sosinski ‐ Dept. of Hort Sci North Carolina State University, Raleigh, NC 27695 
    Vladimir Shulaev  ‐ Virginia Tech & Virginia Bioinformatics Institute Blacksburg, VA 24061‐0477
    Riccardo Velasco  ‐ Biology Department Istituto Agraraio San Michele. Trento, Italy 




                                                                                                       3
Professional interests of RosBREED Advisory Panel members
 RosBREED has three Advisory Panels, representing industry, scientific, and 
 extension interests.  Panel members broadly represent the diversity of Rosaceae by 
 crop, region, and professional expertise. 
 crop region and professional expertise


AP Color Key
  Industry
  E t   i
  Extension
  Scientific



                                       Bierlink
                                       Curtis
                                       Perry
           Alcala                      Sparks
           Cook                        Seavert
           Particka                    Bliss              Watkins
           van Sickle                                     Cheng
           Hirst                                          Simon
                                  Allen    Goldberger
           Abbott
                                  Carr     Olmstead
           Buell
                                  Dodd     Sherman
           Gardiner
                                  Grim     Arus
                                  Korson   Arvai
                        Baugher
                                                     Stokes




                             Moffitt    Karp      Ross




                                                                                       4
                       RosBREED Industry Advisory Panel


   Jim Allen: President 
    New York Apple Association, Fishers, NY (http://www.nyapplecountry.com/)  
    Board of Trustees, US Apple Association (http://www.usapple.org/) 
   Phil Baugher: Co‐owner 
    Adams County Nursery, Aspers, PA (http://www.acnursery.com/)  
    Chair, US Apple Association Research Committee  
   Henry Bierlink: Director 
    Washington Red Raspberry Commission, Lynden, WA (http://www.red‐raspberry.org)  
    National Berry Crops Initiative (http://www.nationalberrycrops.org/) 
   Chalmers Carr III: CEO 
    Titan Peach Farms, Inc., Ridge Spring, SC (http://www.titanfarms.com/default.asp)  
    Board of Directors, South Carolina Peach Council (http://www.scpeach.com/about.htm) 
   Fred Cook:  National Research Department Manager 
    Driscoll’s Strawberry Associates, Watsonville, CA  (http://www.driscolls.com/  ) 
   Robert Curtis: Senior Manager, Production Research 
    Almond Board of California, Modesto, CA (http://www.almondboard.com) 
   Bill Dodd: President 
    Ohio Fruit Growers Marketing Association, Newcomerstown, OH 
    (http://www.ohioapples.com/ohio_apples_fgma.htm) 
    Board of Trustees, US Apple Association
   Bruce Grim: Director 
    Washington Marketing Associations, Wenatchee, WA 
    (http://www.themarketingassociations.org/) 
    Executive Director, Washington State Horticultural Association 
    (http://www.wahort.org/) 
    President, Board of Trustees, US Apple Association
   Philip Korson: President 
    Cherry Marketing Inst., Inc., Lansing, MI (http://www.cherrymkt.org/)  
   Kevin Moffitt: President 
    Pear Bureau Northwest, Portland, OR (http://www.usapears.com/) 
   Chrislyn Particka:  Research Director 
    Sakuma Bros. Inc. Burlington, WA (http://sakumabros.com)
   Tom Stokes: CEO 
    Tree Top Inc, Selah, WA (http://www.treetop.com/) 
    Board of Trustees, US Apple Association 
   Gary van Sickle: Research Director 
    California Tree Fruit Agreement, Reedley, CA, 
    (http://www.eatcaliforniafruit.com/index.asp)
    (h //               lf     f         / d        )




                                                                                           5
                      RosBREED Extension Advisory Panel

   Jessica Goldberger: Asst. Professor, Dept. of Rural Sociology, Washington State Univ.
    Jessica Goldberger: Asst Professor Dept of Rural Sociology Washington State Univ
    Professional interests: agricultural knowledge, science, and technology 
    (http://www.crs.wsu.edu/facstaff/goldberger/goldberger.html) 
   Peter Hirst: Assoc. Professor, Dept. of Horticulture, Purdue Univ. 
    Professional interests: tree fruit production systems and physiology, physiological 
    genetics of flowering and fruit development in Rosaceae; state Extension specialist; 
    undergraduate and graduate education
    undergraduate and graduate education
    (http://www.hort.purdue.edu/hort/people/faculty/hirst.shtml) 
   David Karp: Associate, Agricultural Experiment Station, UC Riverside 
    Professional interests; freelance food journalist and photographer 
   Mercy Olmstead: Asst. Professor, Department of Horticultural Sciences, University of 
    Florida‐IFAS, Professional interests: Stone Fruit Extension Specialist 
    ( p //              /          )
    (http://hos.ufl.edu/stonefruit) 
   Ron Perry: Professor, Dept. of Horticulture, Michigan State Univ. 
    Professional interests: extension and research in cherry and apple orchard systems 
    (http://www.hrt.msu.edu/faculty/perry.htm) 
   Clark Seavert: Professor and Center Director, North Willamette Research and Extension 
    Center, Oregon State Univ. 
    Professional interests: economics, risk management and decision tools for ornamental, 
                                        ,         g                                        ,
    nursery, and berry crops 
    (http://oregonstate.edu/dept/NWREC/Staff/Seavert/Seavert.html) 
   Jamie Sherman: Asst. Professor, Dept. of Plant Sciences and Plant Pathology, Montana 
    State Univ. 
    Professional interests: wheat genetics and Outreach Coordinator for USDA‐NRI 
    WheatCAP 
    (http://plantsciences.montana.edu/facultyorstaff/faculty/sherman/sherman.html) 
   Brian Sparks, Group Editor, American/Western Fruit Grower:   
    Willoughby, OH 
    Professional interests: trade and web‐based publication covering U.S fruit production 
    (http://www.growingproduce.com/americanfruitgrower/) 
   Chris Watkins: Professor, Dept. of Horticulture, Cornell Univ.,  
    Assoc. Director for Cornell Cooperative Extension
    Professional interests; postharvest physiology and management of apple, pear, peach, 
    and cherry; state‐wide agricultural extension activities 
    (http://hort.cals.cornell.edu/people/faculty.cfm?netId=cbw3) 




                                                                                               6
                       RosBREED Scientific Advisory Panel

   Albert Abbott: Professor, Dept of Biological Sciences, Clemson Univ. 
    Research areas; structural genomics of Prunus, sequencing of peach genome 
    (http://www.clemson.edu/cafls/departments/genbiochem/people/aabbott.html) 
   Pere Arús: Head, Plant Genetics Dept., Institut de Recerca i Tecnologia Agroalimentàries 
    (IRTA), Cabrils, Spain 
    Research areas:  molecular tools for plant genetics; comparative genomics and 
                                          p     g              p       g
    application of molecular markers in Rosaceae breeding 
    co‐PD: European Integrated Research Project ISAFRUIT 
    (http://www.isafruit.org/Portal/about.php) 
   Joe Arvai, Professor of Judgment and Decision Science, Michigan State Univ. 
    Research areas: testing theories of decision‐making in the absence of formalized 
    decision support; developing decision aids to improve decision quality 
   Frederick Bliss: Professor Emeritus, Univ. of California, Davis and Sr. Director, R&D 
    Special Projects, Seminis Vegetable Seeds, Woodland, CA 
    Research areas: genomics, genetics, and breeding of fruit and vegetable crops; research 
    administrator in public and private sectors; commercialization of breeding products 
   Robin Buell: Assoc. Professor, Dept. of Plant Biology, Michigan State Univ. 
    Research areas: genomic aspects of plant biology and plant pathogens; high throughput 
    sequencing, functional genomics, comparative genomics, and bioinformatics 
    (http://www.plantbiology.msu.edu/faculty/faculty‐research/robin‐buell/) 
   Lailing Cheng: Assoc. Professor, Dept. of Horticulture, Cornell Univ., Ithaca, NY 
    Research areas: plant nutrition, fruit physiology, molecular, cellular, and whole plant 
    response to abiotic stress in apple, cherry, grape; undergraduate and graduate 
    education (http://hort.cals.cornell.edu/cals/hort/people/faculty.cfm?netId=lc89) 
   Susan Gardiner: Principal Scientist, The Horticultural and Food Research Inst. of New 
    Zealand (Plant & Food Research [PFR]) 
    Research areas: leads Gene Mapping Team integrated with PFR breeders in several 
    Rosaceae crops, including, apple, apple rootstock, pear, peach/nectarine and apricot 
   Carolyn Ross: Asst. Professor, Dept. of Food Science, Washington State Univ. 
    Research areas: sensory analysis and consumer preferences of fruit and fruit products, 
       l d f h d                   d   l           d h         d
    including fresh and processed apple, pear, and cherry and wine 
    (http://sfs.wsu.edu/facultystaff/Ross.html) 
   Phil Simon: Research Geneticist, USDA‐ARS and Professor, Dept. of Horticulture, Univ. of 
    Wisconsin‐Madison 
    Research: vegetable breeding and genetics; biochemistry of culinary and nutritive 
    factors, terpenoids, and sugars in carrots and garlic 
    (htt //       h ti lt        i   d /f lt /f lt li t h )
    (http://www.horticulture.wisc.edu/faculty/facultylist.php)
    Vice Chair: U.S. Plant Breeding Coordinating Committee 
    (http://cuke.hort.ncsu.edu/gpb/pr/pbccmain.html) 




                                                                                                7
                         What need does RosBREED address?
U.S. Rosaceae crop industries face numerous limitations to profitability and 
sustainability.  Overcoming these barriers requires rapid development and deployment 
of new cultivars with improved characteristics to meet dynamic industry and market 
of new cultivars with improved characteristics to meet dynamic industry and market
needs and consumer preferences.  This project seeks to identify breeding trait targets 
based on knowledge of what industry sectors and consumer’s value, and utilize 
genomics information to develop a sustainable technical platform to accelerate and 
increase  the efficiency of cultivar development and adoption. 


                 What crops and traits will be targeted in RosBREED?
RosBREED will focus on fruit quality traits for four rosaceous crops: apple, peach, 
cherry and strawberry.  Market‐based information will be used to objectively quantify 
the importance of fruit quality traits that will be targeted for marker‐assisted breeding.
the importance of fruit quality traits that will be targeted for marker assisted breeding


                               RosBREED core breeders
Apple                                              Peach
Washington State Univ (Kate Evans)
Washington State Univ (Kate Evans)                 Clemson Univ (Ksenija Gasic)
                                                   Clemson Univ (Ksenija Gasic)
Cornell Univ (Susan Brown)                         Univ of Arkansas (John Clark)
Univ of Minnesota (James Luby)                     Texas A&M (David Byrne)
                                                   Univ of California (Tom Gradziel)
Sweet cherry
       g              (            g    )
Washington State Univ (Nnadozie Oraguzie)          Strawberryy
                                                   Michigan State Univ (James Hancock)
Tart Cherry                                        USDA‐ARS Corvallis (Chad Finn)
Michigan State Univ (Amy Iezzoni)                  Univ of New Hampshire (Tom Davis)




                                                                                             8
                                          Why now?
2010 is a watershed year for Rosaceae with the anticipated release of the peach, apple, and 
strawberry genome sequences.  In addition over 250 major genes and marker‐trait associations 
have been identified in rosaceous crops.  Yet a huge gap exists as this genetic information is 
rarely used to improve plant breeding.  RosBREED will bridge that gap and ensure that future 
breeding efforts leverage genomics and genetics information to more efficiently reach the 
breeding efforts leverage genomics and genetics information to more efficiently reach the
cultivar improvement goals.




           What is the technology that will be developed and adopted?

The central technology to be used in this project is marker‐assisted breeding (MAB). An eight‐
stage MAB Pipeline will be established, and demonstrated with markers for high impact traits, to 
assist breeders in improving the efficiency of their programs.  RosBREED will develop the 
community resource of a genome‐scanned, comprehensively phenotyped (for fruit quality) 
reference database on pedigree‐linked breeding germplasm. Pedigree‐Based Analysis will be 
used to functionally characterize alleles at detected QTLs, describing the genotypic value of 
breeding parents.  The genome scans for each of the major crop groups (apple, peach, 
strawberry, and cherry) will exploit ancestral genomic relationships across Rosaceae, and focus 
on shared fruit quality genes.  Software will be developed for breeder access to databases and 
decision‐support analytical tools, incorporating such features as trait economic values, cost‐
decision support analytical tools incorporating such features as trait economic values cost
efficiency calculation of (marker‐assisted) seedling selection schemes, cross planning, and 
breeder‐friendly interfacing of genomics information.




                                                                                                    9
                    Why are we working together as a crop family?

  RosBREED is rooted in our vision that the common ancestry of the diverse 
           g                                  g        g
  Rosaceae genera can be harnessed to leverage knowledge and resources across 
  commodity boundaries.  For example…




                                 the same gene
                              …the same gene 
                              that controls the 
                              presence of red 
                              pigment (skin and 
                              flesh) in apple
                              flesh) in apple 
                              likely controls the 
                              presence of red 
                              pigment in cherry. 


                          What are the targets for success?

The marker‐assisted breeding approach will be adopted and implemented by the core 
breeders as a cost effective strategy for improving the efficiency of their programs. 
Stakeholders will appreciate how the use of genomics information can be harnessed to 
                                            f             f
develop new varieties that meet market needs and consumer preferences. 




                                                                                         10
                                Marker‐Assisted Breeding
 This approach, usually shortened to MAB, uses genetic markers, usually DNA‐based 
tests, to monitor the presence of desirable and undesirable genes in breeding plants. 
RosBREED will focus on enabling breeders of apple, peach, cherry, and strawberry 
breeders to make more informed selection of the best parents to combine and the 
breeders to make more informed selection of the best parents to combine and the
best seedlings to advance in cultivar development.  Selection for improved fruit 
quality traits including texture, size, and flavor will thereby be enabled prior to 
planting young trees or plants in evaluation orchards or field plots, and set the stage 
for similar advances in other Rosaceae crops.

DNA:         The genetic code that controls the structure and function of all 
             organisms, including fruit crops.
Gene:        A specific segment of DNA that codes for a certain trait.
Marker:      A specific DNA segment that is close to a gene of interest and can be 
             used to predict the presence of the gene.
MAPS
MAPS:        Marker‐assisted parent selection
             Marker assisted parent selection
MASS:        Marker‐assisted seedling selection

Apple Texture is an example of an important trait that influences consumer 
preference for apple fruit.  Firm, juicy, crisp‐fleshed apples are more desirable than 
those with soft and mealy flesh.
those with soft and mealy flesh.
                          A gene that influences the maintenance of apple texture is 
                          known – Md‐ACS1. One variant of this gene results in normal 
                          production of the ripening hormone ethylene in fruit.  The 
                          other variant results in much less ethylene, allowing fruit to  
                          maintain excellent texture for longer periods.  A DNA marker 
                          is available that acts like a flag for the Md‐ACS1 gene, 
                          indicating which variant is present.
                          Breeders can now determine which variants each potential 
                          parent has, and thereby design crosses that will yield a large 
                          proportion of seedlings with low ethylene production.  Those 
                          offspring carrying the “wrong” variant can also be weeded 
                          out at the very young seedling stage. The breeders avoid 
                          wasting resources producing and growing inferior trees.

                                                         Selected text courtesy of:

                                                                                             11
                            RosBREED Meetings & Workshops 
                            R BREED M ti      &W k h
Timeline      Meeting            Location                Target Audience                             Theme
                                                     RosBREED Participants,
                              San Diego, CA
            RosBREED I                             Advisory Panel Members, &           RosBREED Orientation & Planning
                              Jan 7-8, 2010
                                                         Collaborators
 YR 1                          Seattle, WA         RosBREED Genomics team               Design genotyping platforms in
            SNP Summit
                                May 2010          participants & Collaborators      coordination with international partners.
                             Palm Springs, CA      Non-core breeders & local           RosBREED Introduction to non-core
           ASHS Workshop
                               August 2010          Advisory Panel Members                 Rosaceous crop breeders
                                                                                    • Breeding teams perform data analysis.
                                           MI
                             East Lansing, M         RosBREED Participants,              •O            f             l
                                                                                           Overview of RosBREED goals,
 YR 2       RosBREED II     Week of March 3rd,     Advisory Panel Members, &         accomplishments, deliverables to date.
                                  2011                   Collaborators                   •Feedback from AP Members &
                                                                                                 Collaborators
                                                                                    • Breeding teams perform data analysis.
                             East Lansing, MI        RosBREED Participants,              • Overview of RosBREED goals,
            RosBREED III    Week of March 5th ,    Advisory Panel Members, &
                                                          y                ,         accomplishments, deliverables to date.
                                                                                            p        ,
 YR 3                             2012                   Collaborators                   •Feedback from AP Members &
                                                                                                 Collaborators
            Participatory       4 locations*       Core and non-core breeders         Assist in MAB adoption by breeders
             Workshops             2012           Local Advisory Panel Members                Stakeholder education
                                                                                    • Breeding teams perform data analysis.
                                                                                          • Overview of RosBREED goals,
                                                     RosBREED Participants,          accomplishments, deliverables to date.
                                    TBD
            RosBREED IV                            Advisory Panel Members, &             •Feedback from AP Members &
                                   2013
                                                         Collaborators                            Collaborators
                                                                                      •Final recommendations and program
 YR 4                                                                                              evaluation
            Participatory       5 locations*       Core and non-core breeders         Assist in MAB adoption by breeders
                 k h
             Workshops             2013                                Members
                                                  Local Advisory Panel M                      Stakeholder education

                                                  Regional extension specialists.               MAB training
              Regional       NY, SC, WA, CA
                                                       Non-core breeders                    Technology transfer
             Workshops            2013
                                                    Industry representatives            Engage stakeholder community




   * Participatory workshops will be located in NY, MI, SC, AR, TX, CA, WA, MN, and NH.

                                                                                                                                12
Dr. Michael Coe
Cedar Lake Research and Consulting LLC
Role: External evaluation of RosBREED outreach acitivies
michael@cedarlakereserch

                                            RosBREED Evaluation of Extension Activities – Narrative Overview
            Group                                                                Overview of Evaluation Approach

                            Several methods will be used to measure integration of genomics and socio-economic information into breeding programs, including
                            knowledge of these methods, related attitudes and skills, and actual use of these methods in plant breeding activities. Baseline levels will
                            be measured during 2010 using written surveys and telephone interviews, which will be repeated in subsequent years to monitor change
                            over time. These surveys and interviews will also be used to obtain feedback about RosBREED sponsored online tools and resources,
                            including RosBREED Community of Practice and Community of Interest activities and publications.
        Breeders and
                            Feedback forms will also be used immediately after workshops, short courses, national and regional meeting presentations or other
       Allied Scientists
                            training sessions, to gather extension audience observations and recommendations on both the content and format of these events.
                            Surveys, interviews and document review will also be used to assess the extent to which the project has resulted in peer reviewed
                            publications that contribute to the knowledge base in genomics and plant breeding; the extent to which online bioinformatics database
                            access and use is improved; the extent to which additional educational opportunities in genome-assisted methods are available to
                            breeders, allied scientists and industry stakeholders.

                            An organization-level survey for these groups will be used to track their awareness and support of marker assisted breeding and the
                            integration of socio-economic research into Rosaceae breeding, as well as the creation and use of new cultivars and related expansion of
                            production and marketing of Rosaceae crops.
     Producers/Processors
                            Industry professionals may also participate in workshops, short courses, regional meetings, or online extension activities. Assessment data
      Marketing groups
                            will also be gathered from these groups using parallel surveys so that their feedback can be compared to that received from breeders,
     Trade organizations    allied scientists, and students.
                            A sample of individuals from these groups will also be asked to participate in an evaluation of RosBREED online extension activities and
                            resources, including RosBREED Community of Practice and Community of Interest activities and publications.

                            Surveys will be used at the beginning and end of graduate student traineeships to gather student observations and recommendations
                            about the usefulness of this program. Additional follow up surveys will be given to graduate students approximately one year after their
                            participation, including questions about the extent to which students have ongoing or increased interest and participation in research,
      Graduate Students     teaching or field practice in marker assisted methods. Document review will include theses, dissertations, peer-reviewed journal articles
                            or other professional publications or presentations by students on related topics.
                            Students may also participate in workshops, short courses, regional meetings, or online extension activities. Student participants will be
                            tracked so that their feedback can be compared to that received from breeders, allied scientists, and industry professionals.




                                                                                                                                                                           13
                                     RosBREED Evaluation of Extension Activities – Data Sources and Timeframe

                                                                                                     Training Event
       Group                            Surveys                          Interviews                                                Other Data Sources
                                                                                                    Feedback Forms

                        2010 Baseline Survey of knowledge,
                                                                     2010 Baseline
                         attitude, skills, aspirations, and
                                                                      Interviews with a
   Breeders and          behaviors related to the integration of
                                                                      sample of breeders
  Allied Scientists      marker assisted methods and socio-
                         economic research in breeding               Follow-up in
                                                                      subsequent years
                        Follow-up in subsequent years
                                                                                                                             Ongoing Document Review will
                                                                                                                             include project-related peer
                                                                                                                             reviewed journal articles, other
                        2010 Baseline Organizational Survey on                             Event Feedback Forms will be     publications, course syllabi,
Producers/Processors     awareness and support of marker                                    systematically used at the end   dissertations, etc.
                         assisted breeding, socio-economic                                  of RosBREED sponsored
 Marketing groups        research, creation and adoption of new                             workshops, courses, and          Participants from all groups
Trade organizations      cultivars                                                          presentations at regional or     may be involved in evaluation
                        Follow-up in subsequent years                                      national meetings.               of online extension activities
                                                                                                                             and resources through usability
                                                                                                                             testing or expert review
                                                                                                                             methods.
                        Baseline at beginning of each
                         traineeship, beginning in 2010
 Graduate Students
                        Follow-up at end of each traineeship
                        Additional follow up in 2012, 2013, 2014




                                                                                                                                                                14
                               RosBREED I Agenda
                             Wednesday, January 6, 2010 – Arrive 
 
Evening ‐ Jim McFerson & Amy Iezzoni will coordinate dinner plans for participants who arrive 
in the afternoon.  
 
       Thursday, January 7, 2010 – (Agenda focuses on involving Advisory Panel Members) 
 
Meeting Location:  Windsor Room, Town and Country Hotel, Mission Valley 
                         San Diego, CA 
 
8:00 – 8:30  Welcome, agenda and self‐introductions – Amy Iezzoni 
 
8:30 – 9:30  Project Overview with time for questions ‐ Amy Iezzoni 
 
9:30 ‐ 9:45  Break 
 
9:45 – 11:45   RosBREED Core breeding programs –Amy Iezzoni moderator. 
 
11:45 – 1:15 Lunch (walk to Mission Valley Food Court) 
 
1:15 – 1:30 Introduction of RosBREED activities – Cameron Peace (Moderator for afternoon 
session) 
session)
 
1:30 – 2:00  Activity 1 Socio‐Economics – Chengyan Yue 
 
2:00 – 3:00 Activities 2‐4 Genomics‐PBA‐BIMS‐MAB Pipeline – Cameron Peace 
 
3:00  3:30 Break
3:00 – 3:30  Break 
 
3:30 ‐  4:00 Activity 5 Extension – Cholani Weebadde 
 
4:00 ‐ 4:15 Activity 5d – Outreach impact evaluation – Michael Coe 
 
4:15 – 5:00 Discussion with AP members ‐ Jim McFerson, moderator
4:15  5:00 Discussion with AP members Jim McFerson, moderator
 
6:00  RosBREED buffet dinner at the Marriott Courtyard San Diego Mission Valley.  Synergy 
Room.   (595 Hotel Circle South) 



                                                                                                 15
                          RosBREED I Agenda con’t
   Friday, January 8 – (Agenda targets within‐project educational and organizational needs) 
 
Meeting Location:  Windsor Room, Town and Country Hotel, Mission Valley 
                        San Diego, CA 
 
8:00 – 8:15 Overview of day’s activities – Jim Luby (Moderator of morning session) 
 
8:15 – 8:45  PediMap demo – Roeland Voorrips 
 
8:45 – 9:45  Introduction to FlexQTL and discussion of software targets – Marco Bink & Eric van 
de Weg 
 
9:45 – 10:15  Break 
 
10:15 – 12:15 Breeder Discussion (Eric, Jim, Dechun, Marco, Nahla, Cameron) Make sure the 
breeders know what their goals are for the 2010 growing season, including PBG goals for 2010.  
 
12:15 – 1:30  Lunch (Walk to Mission Valley Food Court) 
 
    Cholani moderates afternoon session  
 
1:30 – 2:00 PBGworks demo and discussion – Alex Stone (available for Q&A from 2‐3) 
 
2:00 – 4:00 Concurrent activities 
 
    Activity 1: PediMAP hands on sessions with breeders split into 3 groups (Prunus, Malus 
          dF       i ) R l dV          i &E i        d W
       and Fragaria) ‐  Roeland Voorrips & Eric van de Weg
    Activity 2: SE breeder interviews 
    Activity 3:  DNA marker polymorphism discussion – Nahla 
 
4:00 – 5:00 Wrap up – Cholani Weebadde and Audrey Sebolt: Identification of any knowledge 
gaps or organizational items that need to be addressed. Discussion of how progress and 
potential problems should be communicated to the Administrative Team and/or Team Leaders
potential problems should be communicated to the Administrative Team and/or Team Leaders




                                                                                                   16
                                   Advisory Panel Members

                    Questions to be thinking about for our discussion at 4:15 PM

            R BREED build interactive communications on goals, activities, outcomes, and impacts 
1. How can RosBREED b ild i t
1 H                                 ti          i ti        l    ti iti      t         di     t
   that are mutually beneficial to both RosBREED members and stakeholder groups? 

2. How can RosBREED advance the idea that the benefits from RosBREED activities are not defined 
   by state or crop boundaries but should be viewed as regional or national benefits?

3 How can RosBREED communicate goals activities outcomes and impacts to the general public of
3. How can RosBREED communicate goals, activities, outcomes, and impacts to the general public of 
   consumers and tax payers?

4. Are there other projects or on‐going research activities where collaboration with RosBREED
   would produce significant synergies?  What are these opportunities?

5. Are there any methods or plans of work that could be done differently to better meet project 
               y            p                                          y                p j
   goals?

6. Are there any misunderstandings of our project that we need to address (e.g. RosBREED is not 
   about GMOs. RosBREED will only help growers who have access to Club varieties)?

7. What was good about RosBREED I?  

8. What recommendations do you have for next year so we can make RosBREED II the best use of 
   your time?




                                                                                                     17
                    RosBREED Staff

  Audrey Sebolt  Project Assistant for Communications
  Audrey Sebolt – Project Assistant for Communications

Michigan State University
A342‐E PSSB
            g,
East Lansing, MI  48824
517.355.5191 x 1432
Cell:  517.230.5506
grantzau@msu.edu

   Joan Schneider – Project Assistant for Budget

Michigan State University
A288 PSSB
East Lansing, MI  48824
517.355.5191 x 1359
schnei37@msu.edu




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