MONGOLIAN ACADEMY OF SCIENCES Institute of Chemistry and Chemical Technology MONGOLIAN JOURNAL OF CHEMISTRY № 13 (39) Ulaanbaatar 2012 Editorial board: Editorial-in-Chief: Acad. Prof. Batsuren D Dr.Minjigmaa A Members: Acad. Amgalan J Acad. Regdel D Acad. Purevsuren B Acad.Temuujin J Sc.D. Gan-Erdene T Sc.D. Avid B Dr. Odontuya G Dr. Bayarmaa B Dr. Ganbaatar J Dr. Bayarjargal M Dr. Khasbaatar D Dr.Enkhtuul S Prepared by: M.S Otgonsuren D MAS, 4th building, Peace Avenue, Ulaanbaatar 51, Mongolia. Fax: 976-11-453133 email: email@example.com w w w .icct.mas.ac.mn Preface Mongolian Journal of Chemistry provides a unique forum for the publication of significant and original work across a variety of disciplines including chemistry, biology, physics, chemical engineering and material science, which is likely to be of interest to the multidisciplinary community that the journal addresses. Readership-Mong.J.Chem coverage is highly relevant to a variety of industrial and academic sectors including: pharmaceuticals; plant chemistry, analytical science; coal and petrochemistry, bio- and nanotechnology and material science. The Editors at Mongolian Journal of Chemistry are committed to publishing high quality new work which makes a significant contribution to the both academic and industrial sectors development. In order to meet this aim, submitted manuscripts were evaluated by the professional Mong.J.Chem Editors to ensue they meet essential criteria for publication in the journal. We thank you for your submission to our journal and look forward to get another submission next year. Organizing Committee: Dr. Minjigmaa A Dr.Bayarmaa B Dr.Ariunaa A Dr.Otgonjargal E Contents 1. A new humic acid remedy with addition of silver nanoparticles G.P.Alexandrova, G. Dolmaa, U.Enkhbadral, G.L.Grishenko, Sh.Tserenpil, B.G.Sukhov, D.Regdel, B.A.Trofimov 7-11 2. Biochemical study on the meat and oil of Mongolian fishes B.Chantsalnyam, Ch.Otgonbayar, P.Odonmajig , N.Tsevegsuren 12-15 3. Catalase, protease and urease activity in some types of soil D.Purev, J.Bayarmaa, B.Ganchimeg, B.Ankhtsetseg, O.Anumandal 16-18 4. Chemical composition and quality of some river of Mongol Altai G.Erdenechimeg, Munguntsetseg 19-22 5. Coumarins of Peucedanum Baicalense and their cytotoxic activity J. Ganbaatar, E.E.Shults, Т.N.Petrova, M.М. Shakirov, D.Оtgonsuren, E.Munkhbat, D. Badamkhand, А.G. Pokrovskii, G.А. Тоlstikov 23-27 6. Comparative study of essential oil constituents of Bupleurum species from Mongolia Sh.Altantsetseg, S.Shatar, N.Javzmaa 28-30 7. Comparison of hematological and histological analysis of Clinomon and Litovit-M in mice J.Oyuntsetseg, P.Odnoo, J.Ganbaatar, L.Mandakhsaikhan, L.Tsogtzandan 31-36 8. Determination of phenolic compounds in Moravian wines S. Badamtestseg, Ignc Hoza, Pavel Valášek, Vlastimil Kubáň 37-40 9. Determination of Alkaloids from the aerial parts of Caryopteris mongolica Bunge. M.Dumaa, Ya.Gerelt-Od, Zh.Puzhao, L.Yinggang, S.Javzan, D.Selenge, G. Zhang 41-45 10. The comparison between novel reagent H-142, H-143, H-145 and xanthate for flotation behaviour of molybdenum-containing ores Z.Zolzaya, G.Burmaa, S.Enkhtuul, D.Khasbaatar, M.Munkhshur, E.Otgonjargal 46-48 11. Investigation of glucan polysaccharide derived from Fomes fomentarius. ( L ) Fr.grow ing in Mongolia Ts.Bolor, N.Erdenechimeg, B.Amartuvshin, L.Munkhgerel, P.Odonmajig 49-54 12. Investigation of medical mud from Holboolj lake G.Dolmaa, B.Nomintsetseg, G.Ganzaya 55-57 13. Investigation on characterization and liquefaction of coals from Tsaidamnuur and Khoot deposits S.Batbileg , Ya.Dabaajav, B.Purevsuren, J.Namkhainorov 58-65 14. Investigation of phenolic compounds of Scabiosa comosa, structure elucidation of cosmosine D.Batsuren, A.Tuvshintugs, B.Ganpurev, B.Sodbayar, B.Enkhjargal, J.Tunsag 66-71 15. Phytochemical constituents on leaves of Mongolian Sea buckthorn (Hippophae rhamnoides L.) G.Davaakhuu, N.Tsevegsuren, Ts.Bulgan 72-74 16. Phytochemical and pharmacological study of Mongolian Traditional drug GARIDI-5 E.Sugarjav, B.Uuganbayar, Z.Oyun, Z.Ariunaa,G.Davaakhuu, Ch.Chimedragchaa 75-77 17. Preliminary results on characterization of various coal combustion products from Mongolian thermal power stations and their application for preparation of geopolymers A.Minjigmaa, Ts.Zolzaya, B.Davaabal, U.Bayarzul, J.Temuujin 78-81 18. Protein and amino acid compositions in some Mongolian wood-rotting fungi Sh.Naranmandakh 82-86 19. Removal characteristics of arsenic by Fe-Mn layered double hydroxide and double oxide E.Otgonjargal, Kitae Baek, Ts.Batjargal, Jung-Seok Yang 87-97 20. Relationship between structure and thermodynamic activity of carbon black A.M.Amdur, V.V.Pavlov, B.Purevsuren, L.Munkhtuul 98-101 21. Some phytochemical constutients and bioactivity of water- and ethanolic extract of Rumex acetosella L D.Punsaldulam, E.Munkhbat, D.Otgonsuren 102-107 22. Synthesis and their photodynamic activity of new Photosensitizers for PDT B.Bayarmaa, Young Key Shim 108-113 23. Synthesis and catalytic test of bimetallic Pd-Ag nanoparticle prepared by the impregnation method Sh.Nyamdelger, G.Burmaa, Christian Weilach and Karin Föttinger 114-119 24. Spectroscopic study and implications for biological reactive oxygen space sensing of the inorganic DMSO/POCl3 reaction with BODIPY N.Amgalan, Taehong Jun, Kang Mun Lee, HyuDavid, G.Churchill 120-122 25. Study of biological activity compounds in some Mongolian medicinal plants S.Bayaraa, J.Batkhuu, A.Byayanmunkh, L.Khurelbaatar 123-124 26. Study biological activity of alkaloid protopine S.Javzan, Y.Jamyansan, R.I.Alexandrova, D.S.Bae , Ch.W.Nho 125-133 27. Study on the washability of Baganuur coal by using float sink test A.Ariunaa, G.Tsatsral, J.Narangerel, R.Erdenechimeg, J.Dugarjav, B.Purevsuren, S.Jargalmaa 134-138 28. Study on biological activity of Hyoscyamus niger L. grown in Mongolia J.Irekhbayar, G.Davaakhuu, S.Odontuya, Burm-Jong Lee 139-142 29. Use of modifed zeolites for chromium removal from tannery wastewater D.Batgerel, S.Erkhembayar, E.Anudari, G.Ariunzul 143-145 A new humic acid remedy with addition of silver nanoparticles G.P. Alexandrova1, G. Dolmaa2, U.Enkhbadral3, G.L.Grishenko1, Sh.Tserenpil2, B.G. Sukhov1, D. Regdel1, B.A.Trofimov1 1 Irkutsk Institute of Chemistry, Siberian Branch of RAS, Irkutsk, Russia 2 Institute of Chemistry and Chemical Technology, MAS, Ulaanbaatar, Mongolia e-mail:firstname.lastname@example.org ABSTRACT: Previously known biogenic stimulator humic acid (HA) was the subject of this current study and HA based new remediation was developed by addition of silver (Ag) nanoparticles in its macromolecule. Extracted HA from a healing mud was characterized and used as reducing agent for Ag ion as well as a stabilizer for the formed Ag nanoparticles. The properties of the obtained hybrid composite were examined by XRD, UV and FTIR spectroscopic techniques. The diameter of the nanoparticles in the HA polymer was up to 8.6 nm and they were identified to be metallic Ag. Keywords: Composite; Healing mud; Humic acid; Silver nanoparticles INTRODUCTION biomaterials, as well as film castings for A romatic nucleus and functional groups electronic and optoelectronic devices. The in the HA molecule react with cell walls formation of Ag nanoparticiples in a variety of of organisms and with receptors in a polymers (e.g., natural and syntetic) has been derma; furthermore, it shows a therapeutic examined as size and dispersion pattern of metal effect by penetrating through tissues . HA is particles were unique for each case. Also natural a major proportion of organic matters in polymers are biocompatible making them Mongolian healing mud  and it tends to form particularly interesting for researchers. an organomineral particles. A hypothesis, For obtaining metal nanoparticles, the most therefore, was developed that HA may possess a important controlling parameters include stabilizing character for metal nanoparticles as reducing agent type, stabilizer and the matrix in other natural polymers do [3-4] and such which nanoparticles are formed. Dendrimers behaviour has been recently investigated . usually take on a spherical three dimensional Humic substances from the healing mud differ structure, which is very different from linear from coal, turf and soil derived counterparts by polymers. Additionally, it is predicted that HA their low molecular weight and high content of may provide more active sites for stabilization reactive functional groups . Silver shows an of metal nanoparticles. antibacterial characteristic in addition to its Akaihge et al examined the formation of Ag chemical stability, good thermal and electrical nanoparticle in the presence of HA (i.e, from the conductivity and catalytic properties. Therefore fresh water and marine sediment) under organic and inorganic hydrid composites environmentally relevant conditions (e.g., bearing Ag nanoparticles were synthesized and concentration, pH and temperature), and studied for a different purposes; for instance, as discussed the possible formation by the natural medical preparations, antimicrobial coatings for processes and their stability depending on HA 7 Biochemical study on the meat and oil of Mongolian fishes B.Chantsalnyam1, Ch.Otgonbayar1, P.Odonmajig1, N.Tsevegsuren2 1 Institute of Chemistry and Chemical Technology, MAS 2 School of Chemistry and Chemical Engineering, NUM e-mail:email@example.com ABSTRACT: The objective of this study was to determine the meat quality and fish oil’s physical and chemical characteristics of the Mongolian fishes: Syrok (Coregonus peled), and Lenok (Brachymystax lenok). Lipid content was 3.1 to 14.15%, moisture was 72.14 to 80.5%, protein content was 8.54 to 9.8% and total mineral element was 0.5 to 3.1%. The caloric value Lenok (Brachymystax lenok) was 68.7 and Syrok (Coregonus peled) - 142.8 Kj/100g, respectively. Minerals included potassium (46.32 to 52.22%), phosphorous (39.06 to 41.3%), calcium (3.26 to 5.87%), magnesium (2.08 to 3.97%) and silicon (1.14 to 2.24%), while zinc, iron and copper were present intrace amounts. Eight non essential amino acids with total amount of 58.61 to 59.64% were identified, and histidine detected as in highest value in all the species, followed by arginine. The data showed that the Mongolian fishes are of high nutritional value and good source of proteins, minerals as well as non essential amino acids. The physical and chemical characteristics of Syrok (Coregonus peled), and Lenok (Brachymystax lenok) fish oil were analyzed. These parameters are: iodine value (IV), peroxide value (PV), acid value (AV), saponification value (SV), percentage of free fatty acid (%FFA), refractive index (RI) and colour. The extracted lipid content obtained from muscles of Syrok and Lenok was about 14.15% and 3.1%. The percentages of unsaturated fatty acids were higher than saturated fatty acids accounting for 74.37% and 25.6%, 83.83% and 16.46% respectively. Keywords: Syrok (Coregonus peled), Lenok (Brachymystax lenok), protein, fish oil, macro- and micro-elements, amino acid, infrared spectrophotometer INTRODUCTION hypolipidemic agent an antiarthristic agent) . F ish and other sea foods remain an important source of white meat for the human diet to its beneficial effect in reducing coronary heart diseases. This effects especially, due to the fat for the fish. Fishes It is known to be that Mongolia is rich in fishes. From ancient times Mongolian people have been used fish oil as food and remedy in traditional and physical treatment of some illnesses. Therefore, we have used fish Syrok living in fresh water and ocean are contained (Coregonus peled) and Lenok (Brachymystax oils which used as food and in medicine. Fresh Lenok) which are widely distributed in water’s and ocean’s fish oil are contained 14-35 Mongolia. The purpose of our study was highly unsaturated fatty acids. The fatty acid biochemical study of meat obtained from two pattern of triacylglycerol and phospholipids of species of Syrok (Coregonus peled) and Lenok various fish oils were also assessed . (Brachymystax Lenok) Epidemiological studies on the last assure that omega-3 fatty acids are derived from fish and EXPERIMENTAL fish oil to decrease the risk of coronary heart Methods. Moisture content was determined by disease, hypertension and stroke, and their gravimetric method. The ash content was complications . The liver obtained from fish determined by incinerating in a muffle furnance has high pharmacological activity (serve as at 5500C. Composition of the ash was measured 12 Catalase, protease and urease activity in some types of soil D.Purev, J.Bayarmaa, B.Ganchimeg, B.Ankhtsetseg, O.Anumandal School of Biology and Biotechnology, NUM e-mail: firstname.lastname@example.org ABSTRACT: Enzymes in the soil are closely related to the physical, chemical and biological characteristics of the soil and regulate the formation of soil fertility, organic matter, nutrient mineralization and cycling in nature. Soil contains phosphatase, urease, dehydrogenase, catalase, peroxidase, saccharase, protease, amylase, β-glucosidase, arylsulphatase and other enzymes. The purpose of the given work was estimation of catalase protease and urease activity on three different types of soil as high mountained steppe and dry steppe roughly humusified soil; mealy brown carbonated soil and mountain meadow forest soil which are common in the mountain and steppe regions of our country in correlation with soil depth and anthropogenic impacts. In all soil samples the highest enzymatic activities were detected in the depth of 0-15 cm from the soil surface. For all soil samples the enzymes activities decreased more rapidly with increasing soil depth affected to anthropogenic impacts in comparison with samples not exposed to human impacts. Keywords: soil enzymes, catalase, protease, urease, anthropogenic impacts INTRODUCTION depend on microbial community of soils and E nzymes in soils mainly come from plants, soil animals and microorganisms and connected covalently, crosslinked, copolymerized, adsorbed and included in the microcapsules of soil particles (Girish S., Ajit expresses the biological activity of the soil. The enzyme associated with soil fertility, regulating the amount of available nitrogen in plant and plant growth. (Girish S.et al., 2011). Peroxide in soil is formed during respiration and oxidation V., 2011). Soil enzyme activities vary of organic matter (Andersen D.W., Gregorich seasonally and have been related to soil E.G., 1980). Catalase (EC 184.108.40.206) decomposes physiсo-chemical characters, microbial peroxide and its activity depends from organic community structure, vegetation, disturbance oxygen concentration, microbe biomass, and succession (Caldwell B. A. (2005). That is changes in CO2, and depends from why soil enzymes play an important role in dehydrogenase, amidase, glucosidase and formation, converting and decomposition of esterase activity in soils. Therefore an important organic matter to the plant digestible forms, indicator of soil fertility and aerobic decomposition of xenobiotics, involved in the microorganisms (Burns R.G., 1982). Urease (EC nitrogen and other elements cycle and life 220.127.116.11) activity in soils depends from organic cycling of soil microorganisms (Schaller K., and inorganic matter content; especially urease 2009). Nowaday soil enzyme activities have is very sensitive to heavy metals (Girish S.et al., been used as indicators in evaluation of soil 2011). quality, climate changes, destruction and toxification in ecosystems. Protease (EC 3.4.4…) EXPERIMENTAL in soil plays a significant role in nitrogen Three types of soils samples were used in our mineralization, more active in soils with a high study (table 1). In all soil samples moisture, water and humus content forest soils and soluble protein, protease, catalase and urease landfills. Activity of this enzyme does not activities were estimated. 16 Chemical composition and quality of some river of Mongol Altai G.Erdenechimeg1, Munguntsetseg2 1 School of Pharmacy, HSUM 2 Faculty of Chemistry, NUM e-mail:Chimge_5@yahoo.com ABSTRACT: Main ions and guality of Hovd River and, its subrivers were studied. Keywords: Hovd River, main ions, mineralization INTRODUCTION EXPERIMENTAL T he surface water of Mongolia is classified Hydrochemical studies of the water samples were into three groups according to its watershed; done using methods of O.A.Alekin (1973), Arctic Ocean, Pacific Ocean and Continental U.U.Lurie (1971-1947) V.Leite (1975), watershed. The continental watershed involves 68 % L.P.Sokolova (1989), L.A.Reznikov (1970), of the surface area of Mongolia and 40 % of water D.Darimaa (1985), A.Munguntsetseg (2003). We capacity. The system of Khovd River is in Khyargas have taken samples from the points of Khovd river Lake Basin. The system of Khovd River involves 99 and its subrivers, and determined the location of % of surface area of Bayan-Ulgii province, 21.0 % groundwater by JPS until October, 2010. We did of surface area of Khovd province and 21.0 % of chemical composition analysis complex in Barnayl surface area of Uvs province. Khovd river is the Laboratory of water institution in Russia seventh longest and the fifth largest river in Mongolia. Its source is the top glacials of Altai Tavan Bogd Mountains and it flows to Khar-Us Lake. Several other rivers, Tsagaan, Ikh, Uigar, Sogoo, Sagsai, Khatuu, Khavchig, Shijigt, Uliastai, Shurag, Shiver, whose sources are in the glacials of Altai Tavan Bogd mountains flow to Khovd river. G.N. Potanin /1883/, B.A. Smernov /1932/, N.D.Bespalov /1951/, Sh.Luwsandorj /1959, 1968/, D.Dawaasuren /1961/, N.Tsend, Sh.Sodnombaljer /1964/, J.Tserensodnom /1971, 1975/, B. Tsendee /1980/, B. Ariyadagva /1985/ did hydrochemical studies from the points of the sample from Hovd river. According to the classification of O.A.Alekin from that research result Khovd river and its subrivers and lakes waters belong to the group of hydrocarbonate with low mineralization and low hardness water. Beside that do some research and summerize some materials, determine the composition water from Mongol Altai mointain, water gualified estimation, defermine pollution source, collect some etalon materials. Figure 1. The points of the sample 19 Coumarins of Peucedanum baicalense and cytotoxic activity of some isolated coumarins J.Ganbaatar1, E.E.Shults2, Т.N.Petrova2, M.М.Shakirov2, D.Оtgonsuren1, E.Munkhbat1, D.Badamkhand1, G.A.Tolstikov2, D.Batsuren1 1 Institute of Chemistry and Chemical Technology, MAS 2 Novosibirsk Institute of Organic Chemistry SO RAN ABSTRACT: It was shown that the plant Peucedanum baicalense (Redow.) Koch is the source of valuable coumarins. Five linear furocoumarins – isoimperatorin, fellopterin, 8-(1,1-dimethylallyloxy) bergapten, deltoin and marmesin were isolated from the roots of Peucedanum baicalense (Redow.) Koch. The structures of these compounds were elucidated by spectroscopic methods. The cytotoxicity of isoimperatorin, fellopterin, 8-(1,1-dimethylallyloxy)bergapten and deltoin was studied on models of human СEM-13, MT-4 and U-937 tumor cells. Compound 8-(1,1- dimethylallyloxy)bergapten had the greatest cytotoxicity. Keywords: Peucedanum baicalense, furocoumarins, isoimperatorin, fellopterin, 8-(1,1-dimethylallyloxy)bergapten, deltoin, cytotoxic activity, tumor cells, X-ray analysis INTRODUCTION Mongolian territory . To our knowledge a P lants of the genus Peucedanum sp. attract an attention of many researchers as a source of coumarins. Previously, we isolated pyranocoumarin (+)-pterixin from Peucedanum terebinthaceum Fischer et Turcz. systematic phytochemical investigation of this plant has not been properly carried out yet. The aim of this study was to investigate coumarins of Peucedanum baicalense (Redow.) Koch and cytoxic activity some of the pure coumarins. of the Mongolian flora . Plants belonging to Peucedanum species characterized as the source EXPERIMENTAL of angular furocoumarins . Coumarins are Roots of P. baicalense were considered as phytoalexins since plants produce collected near the place Baruun buren, Selenge them as defence substances when wounded or aimag, Mongolia in its butonization-flowering attacked by other organisms. Coumarins can be period in 2012. suggested to be beneficial for the plants . Air-dried ground themselves as natural biocontrolling roots of Peucedanum baicalense (Redow.) Koch antipathogenic compounds as well as for (400 g) were exhaustively extracted by humans as remedy for hyperproliferative skin maceration with 96% EtOH (3x500 ml) at room deseases and as reference compounds in various temperature. The EtOH extract was evaporated bioactive tests. Furthermore, coumarin to an aqueous residue, which diluted with containing plants are valuable as dietary distilled water (1:1), and filtered. The filtrate supplements on the basis of their mild was fractionated by solvents with increasing antimicrobial and anti-inflammatory effects. polarity, i. e., n-hexane, diethyl ether and Coumarins are also active in a plant ethylacetate, respectively. Each fraction was metabolism, taking part in growth regulation. condensed by a rotatory evaporator. Then, the Peucedanum baicalense (Redow.) Koch is a fractions were separated by column plant which widely spread throughout the chromatography over silica gel. 23 Comparative study of essential oil constituents of Bupleurum species from Mongolia Sh.Altantsetseg, S.Shatar, N.Javzmaa Institute of Chemistry and Chemical Ttechnology, MAS e-mail: email@example.com ABSTRACT: Essential oils have been isolated from the Mongolian plant species Bupleurum scorzonerifolium Willd, Bupleurum sibiricum Vest and Bupleurum multinerve DC by a hydro distillation method. The compositions of the essentials oils were analyzed by GC and GC-MS methods. Indicating the following main constituents: limonene (15.21%), p-cymene (11.54%), (E)-β-ocimene (10.50%), myrcene (8.09%), sabinene (6.56%), α-pinene (6.33%), germacrene-D (4.08%), β-pinene (3.13%), δ-cadinene (2.73%), spathulenol (2.71%) and caryophyllene oxide (2.53%) for B.scorzonerifolium; caryophyllene oxide (16.97%), spathulenol (7.25%), pentodecane (6.33%), α-pinene (4.20%), limonene (3.30%), myrcene (3.09%), cis-carvylacetate (1.69%) and β-cubebene (1.43%) for B.sibiricum; germacrene-D (19.41%), (E)-β-ocimene (18.63%), myrcene (9.13%), limonene (7.81%), E-caryophyllene (4.60%), sabinene (2.80%), β-elemene (2.72%), bicyclogermacrene, β-pinene, γ-terpinene, (Z)- β-ocimene (2.00-2.10%), and caryophyllene oxide (1.11%) for B.multinerve. Keywords: Bupleurum scorzonerifolium, B.sibiricum, B.multinerve (Apiaceae), essential oil composition INTRODUCTION cardiovascular diseases, in addition to infectious diseases4. A literature search is revealed many T he genus Bupleurum is a very common wild growing perennial and 150 species of those originate from Euro-Asia. The roots are collected in the spring and autumn of those bitter herbs are used in herbal medicine references of previous work those are on the essential oils of Bupleurum species[6-8]. Volatile components of Bupleurum scorzonerifolium and Bupleurum sibiricum from that contain rutin, quercetin, organic acids the Mongolian flora have been (oleic, linolenic, palmetic, stearic et al), investigated[9,10] previously, while there is no stigmasterol and bupleuromol, as well as report on the volatile constituents of the aerial calcium and potassium[2.3]. parts of Bupleurum multinerve. The Bupleurum root is considered an herbal The aim of this paper was to investigate the anti-inflammatory and antibacterial agent that chemical compositions of the essential oils of may be useful for treating rheumatoid arthritis B.scorzonerifolium, B.sibiricum and as well as it is a popular plant in traditional B.multinerve from the Mongolian forest-steppe medical systems in Europe and Asia with region. reputation in treatment of chronic enlarged liver or spleen which are caused by chemicals and EXPERIMENTAL other hepatic affects, liver stasis, liver Plant material. The aerial parts of the congestion, depression and irregular B.sibiricum Vest were collected from Zuun- menstruation. haraa of the Selenge aimag, B.multinerve DC In Mongolian traditional therapy, the aerial and B. scorzonerifolium Willd were collected parts of Bupleurum species are used in some from Terelj, Hentii mountains in Mongolia 28 Comparison of hematological and histological analysis of “Clinomon” and “Litovit-M” in mice J.Oyuntsetseg1, P.Odnoo2, J.Ganbaatar1, L.Mandakhsaikhan1 L.Tsogtzandan2 1 Institute of Chemistry and Chemical Technology, MAS 2 Mongolian International University ABSTRACT: Based on earlier studies we activated Mongolian Tsagaantsav zeolite by tribo-mechanical avtivation and utilized it as a food additive, giving it the name Clinomon. This research is devoted to investigation whether the supplementations of 2 levels (0.5%and 10%) of Clinomon and Litovit-M in the concentrate feed of mice have any effects on their hematological parameters and compare parameters of Clinomon and Litovit-M. Keywords: Tsagaantsav zeolite, Clinomon, Litovit-M, clinoptilolite, dietary supplement , hematological parameters, histo-pathology analysis. INTRODUCTION clinoptilolite powders. Two mice were M ongolia is rich in many types of mineral resources; zeolite which has unique properties is deservedly one of them. Zeolite from the Mongolian Tsagaantsav deposit has certain advantages like having sacrificed from each group after 10, 20 and 30 days, and blood samples from these mice were collected for hematological parameters and liver and kidney were obtained for histopathological analysis. The mice fed Clinomon and Litovit-M similar chemical composition and physico- didn’t lose weight. Liver and kidney chemical properties to zeolite from the Russian histopathology were not affected. Clinomon, Kholinskii deposit. Based on earlier studies we made by Mongolian Tsagaantsav deposit’s activated Mongolian Tsagaantsav zeolite by clinoptilolite and Litovit-M, made by Russian tribo-mechanical avtivation and utilized it as a Kholinskii deposit’s clinoptilolite at the levels food additive, giving it the name Clinomon. The of 0.5% and 10% in the concentrates doesn’t aim of this research was to investigate whether have any adverse effect on RBC, WBC and the supplementations of 2 levels (0.5%and 10%) HGB. From these results, we concluded that of Clinomon and Litovit-M in the concentrate there were no significant differences between feed of mice have any effects on their Clinomon and Litovit-M on hematological and hematological parameters and compare histological analysis. In further, we should study parameters of Clinomon and Litovit-M. it in long term with large variants animals. Clinoptilolite were added to the standard mouse This paper was based on the animal welfare ration. Four preparations differing in particle application of zeolite. We focused more on size were tested: grilled by vibration mill (VC), previous studies about biomedicine and the natural clinoptilolite (NC), tribomechanically animal nutrition of zeolite. Various biomedical micronized clinoptilolite by attritor mill, named applications of natural zeolite. Clinoptilolite is Clinomon (MC) and Russian dietary non-toxic and safe for use in human and supplement Litovit-M (LM). A total of 54 CBA veterinary medicine. Animal fodder containing strain mice were divided into 9 groups (n=6) zeolites has been shown to increase biomass and supplied with food containing 0.5% or 10% production in fisheries , to promote weight 31 Determination of phenolic compounds in Moravian wines S.Badamtestseg1, Ignc Hoza2, Pavel Valбšek2, Vlastimil Kubбň3 1 Department of Biological Active Products, Drug Research Institute, Ulaanbaatar, Mongolia 2 Department of Biochemistry and Food Analysis, Tomas Bata University, Zlin, Czech Republic 3 Institute of Chemistry, Department of Analytical Chemistry, Masaryk University, Brno, Czech Republic e-mail:Badamtsetseg.firstname.lastname@example.org ABSTRACT: Wines were made from Grьner Veltliner and Zweigelt and cultivars were grown in four different geographical regions of Austria and Czech Republic; two wineries in Austria (Poysdorf, GroЯriedenthal) and two wineries in the Czech Republic (Velkě Bнlovice, Bošovice). Eleven individual phenolics were quantified using a HPLC/UV-VIS method. Keywords: Moravian wine, phenolics, HPLC/UV-VIS, phenolic acids INTRODUCTION The phenolic profiles in wine depend on the phenols contained in the grapes, the extraction T he phenols compounds in wine include a large group of several hundred chemical compounds, known as polyphenols that affect the taste, color and mouthfeel of wine. This large group can be broadly separated into parameters, yeast strain, processing enzymes, cap management, and alcohol concentration [4,5], while the phenolic compounds of grapes are affected by many factors such as genetic variation, maturity, climatic and geographical two categories such as flavonoids and non- conditions [6,7]. Other factors that influence the flavonoids. Flavonoids include anthocyanins extent of phenolic extraction are the molecular and tannins which contribute to the color and weight, size and type of phenolic molecules, the mouthfeel of the wine. Non-flavonoids include surface area for the concentration gradient, other stilbenes sach as resveratrol and compounds temperature treatments including grape and derived from acids in wine like benzoic, caffeic must freezing and thermovinification, and and cinnamic acid. factors that affect cell permeability . White wine contains significantly lower In this study some flavonoids, phenolic acids, amounts of total polyphenols compared with red flavonols and resveratrol were determined. wines, mainly hydroxycinnamic acids, These compounds could be key agents of the hydroxybenzoic acids and flavan-3-ols antioxidant action on the human metabolism . As a material for winemaking, the phenolic pathway, the reason why we wanted to obtain compounds of wine grape are one of the most indication to qualify the wine from a nutritional important aspects determining wine quality. A point of view. Also, the environmental large number of published works have focused condition (temperature, rainfall/humidity, high on the essential contributions of phenolic above sea level and geochemical characteristics) profiles to wine quality and sensory properties can affect the wine maturation and consequently [2, 3]. the concentration of its phenolic compounds. 37 Two new alkaloids from the aerial parts of Caryopteris mongolica Bunge. M.Dumaa1, Ya.Gerelt-Od1, Zh.Puzhao2, L.Yinggang2, S.Javzan1, D.Selenge1, G.Zhang2 1 Institute of Chemistry and Chemical Technology, MAS, Ulaanbaatar, Mongolia 2 Chengdu Institute of Biology, CHAS, Chengdu, China ABSTRACT: Two new alkaloids moncaryopterine A and moncaryopterine B were isolated from the aerial parts of Caryopteris mongolica Bunge. by the column chromatography and HPLC methods. Molecular structures of them were elucidated by MS, 1H, 13C, HSQC, HMBC, 1H/1H COSY, and 1H/1H NOESY NMR methods. Keywords: Caryopteris mongolica Bunge. Verbenaceae, alkaloids, moncaryopterine A, moncaryopterine B INTRODUCTION Caryopteris iridoids, steroid glucosides, C aryopteris mongolica Bunge. is a phenylethanoids, diterpenoids, phenolic acids, deciduous shrub and belongs to the α-caryopterone, a new pyranojuglone, Verbenaceae family. It is widely clandonoside and its acetylated derivatives have distributed throughout the Mongolian territory been isolated, respectively [10, 11, 12, 13, 14]. . In addition, this plant species grows in some To the best of our knowledge there are no data provinces of Hebei, Shanxi of Inner Mongolia on alkaloids in all species of Caryopteris. and Gansu, China . In fact, Caryopteris However, we are reporting here of the mongolica is only species grown in Mongolia, molecular structure elucidation of two alkaloids whereas about 16 other species are discovered isolated from the aerial parts of C. mongolica. in different places of the world. In Mongolian traditional medicine aerial parts EXPERIMENTAL of C. mongolica have been used for the Plant material. The aerial parts of Caryopteris treatment of haemorrhage, chronic bronchitis mongolica Bunge. were collected from Terelj and for an increasing of the muscle strength and Mountain chains, vicinity of Ulaanbaatar, urinary excretion . In Chinese folk medicine during the flowering period in August 2010. Dr. Caryopteris terniflora has been used as B. Mandakh, Institute of Botany, MAS has antipyretic, expectorant and for the treatment of identified the plant species and voucher tuberculosis, rheumatism and cold . Some specimen was deposited at the Herbariums of species of Caryopteris are cultivated for a the Natural Products Chemistry Laboratory, decoration arrangement and ornamental Institute of Chemistry and Chemical purposes. Technology of the Mongolian Academy of Previous chemical investigations of Caryopteris Sciences. mongolica showed the presence of essential Extraction and isolation of alkaloids. The air oils, mono and sesquiterpenoids , hypolaetin- dried and powdered aerial parts (3.3 kg) of 7-glucoside , iridiod glucosides and steriods Caryopteris mongolica were extracted with [7, 8, 9]. Moreover, from other species of 94% ethanol at room temperature for 3 times. 41 The comparison between novel reagent H-142, H-143, H-145 and xanthate for flotation behaviour of molybdenum-containing ores Z.Zolzaya, G.Burmaa, S.Enkhtuul, D.Khasbaatar, M.Munkhshur, E.Otgonjargal Institute of Chemistry and Chemical Technology, MAS ABSTRACT: In this study, a novel flotation collector reagents were tested for molybdenum containing ore. H-142 (E)- 4-[(2-hydroxyethyl)amino]-3-penten-2-one, H-143 (Z)-4-[(2-[(E)-1-methyl-3-oxo-1-byteny]) aminoethyl) amino]-3- penten-2-one and H-145 (E)-4-[(6-[(E)-1-methyl-3-oxo-1-butenyl] aminohexyl) amino]-3-penten-2-one collector reagents were used in flotation process for molybdenum containing ore. Maximum metal recoveries of molybdenum were 63.61%, 67.80% and 68.13%, respectively. Molybdenum recovery of traditional collector butyl xanthate was 15.8%. Keywords: Synthetic reagent, Flotation, Molybdenum, Collector INTRODUCTION different capacity are used in the flotation M olybdenum does not naturally occur as a free metal on the Earth, but rather in various states in minerals, which is most frequently used as an alloying addition in alloy and stainless steels . Its alloying process. Flotation chemicals are divided as collector, coordinator and foaming according to their purpose. Here, we present the possibility use of novel collector reagents such as (E)-4-[(2- versatility is unmatched because its addition hydroxyethyl)amino]-3-penten-2-one (H-142), enhances strength, hardenability, weldability, (Z)-4-[(2-[(E)-1-methyl-3-oxo-1-byteny]) toughness, elevated temperature strength and aminoethyl) amino]-3-penten-2-one (H-143) corrosion resistance. and (E)-4-[(6-[(E)-1-methyl-3-oxo-1-butenyl] Approximately, 50% all molybdenum aminohexyl ) amino]-3-penten-2-one) (H-145) production comes from Cu-Mo ore as a by- that recover the molybdenum from the Cu-Mo product . Mongolia is a major producer and ore and compare the result with the traditional exporter of copper and molybdenum collector BX. concentrate, accounting for 1.2% of the world's molybdenum production . Molybdenum EXPERIMENTAL recovery of Erdenet mining what is only one Main analysis and experiments were carried out Cu-Mo mining industry in Mongolia is only in the laboratory of precious and rare metals, 45%. It needs to find a way to increase Institute of Chemistry and Chemical molybdenum recovery. One of main minerals of Technology, Mongolian Academy of Sciences molybdenum is molybdenite (MoS2) that begins and Central Laboratory of Geology. Samples from primary process of geochemistry. were prepared from copper and molybdenum Flotation means to concentrate the minerals ore of Erdenet mining in 2007. Novel reagents according to their physical and chemical H-142, H-143 and H-145 as collectors that diversity of surface, and specific character to be synthesized in the Irkutsk Institute of Organic soaked with water. A several chemicals of 46 Investigation of glucan polysaccharides derived from Fomes fomentarius (L.) Fr. grown in Mongolia Ts.Bolor, N.Erdenechimeg, B.Amartuvshin, L.Munkhgerel, P.Odonmajig 1 Institute of Chemistry and Chemical Technology, MAS e-mail: email@example.com ABSTRACT: This study was aimed to analyze chemical composition and to isolate the glucan polysaccharides of fruiting bodies of F.fomentarius (L.) Fr. grown in Mongolia. Extraction with hot water (yield 0.91%) and alkali- soluble extraction (yield 3.7%) were purified and examined by using PC, IR and viscometric analysis, respectively. Water fraction showed mainly glucose, rhamnose and arabinose. This preparation contained 0.16% protein and molecular weights were about 6829.3 Da. And Alkali- soluble extraction consist of glucose and rhamnose. The molecular weight of these polysaccharides was 507.9 Da and contained 0.2% protein. Element analysis of Fomes fomentarius (L.) Fr. showed that this fungus contains K, Mg, Ca, Zn and other elements favoring health. Keywords: Fomes fomentarius ( L.) Fr., polysaccharide, monosaccharide, glucan INTRODUCTION antioxidant, anti-inﬂammatory, antidiabetic and M edicinal mushrooms have been widely used as tonic food and herb remedy in many Asian countries since ancient times. They comprise a vast and yet largely untapped source of powerful new antitumor activities [1, 3, 4]. As a result of its perceived health beneﬁts, F.fomentarius has gained wide popularity as an eﬀective medicine and has become one of the valuable mushrooms in China. The fall in supply and the increase in pharmaceutical products and represent a source demand have stimulated interest in the search of polysaccharides with antitumor and for substitutes of the natural products of the immunostimulating properties [1, 2]. Therefore, fungus [5-10]. Also, methanol extract of searching for alternative medicines and natural F.fomentarius had immunomodulatory activity therapeutics for cancer therapy, medicinal . Wasser S.P and Weis A.L have shown that mushrooms are among the most promising the beta-glucans from fruiting body and targets. Fomes fomentarius (Tender polypore) submerged and cultured mycelial biomass of is a fungus of the Polyporaceae family. It is also F.fomentarius had antitumor activity . used to make a popular drink, which is believed Mushroom’s polysaccharides, especially, in Japan to be a tonic and to have an anticancer the high molecular weight β-D-glucan have effect. It has also been reported that Fomes been considered to have anti-cancer activity. fomentarius has an inhibitory effect of virus Many polysaccharides (glucans) purified from infection on plants . The fruiting body has mushroom fungi belong to either homoglycans been used as a traditional Chinese medicine for or heteroglycans while others mostly bind to many centuries for the treatment of various protein residues as polysaccharide-protein diseases, including oral ulcer, gastro enteric complexes. The fungal antitumor disorder, hepatocirrhosis, inﬂammation, and polysaccharides are mainly present as glucans various cancers. Recent study has shown that with different types of glycosidic linkages, but F.fomentarius has the effect of being some are true heteroglycans. Glucan from the 49 Investigation of medical mud from Holboolj Lake G.Dolmaa, B.Nomintsetseg, G.Ganzaya Institute of Chemistry and Chemical Technology, MAS e-mail: firstname.lastname@example.org ABSTRACT: We have studied physicochemical properties and yield of free and linked organic compound, mineral components, element and yield of macromolecular compounds of healing mud, which is collected from Holboolj Lake. Minerals enter peloids underground as well as from the surface. They are leached out of the surrounding earth into the peloid bed. We determined microcline -K(Si3AI)O8, quartz- SiO2 which is most common mineral on the face of earth and albite - Na (AISi3O8) by X-Ray diffraction analysis. The organic matter of medical mud investigated using chemical analysis and analytical technique. Some classes of biomolecules including humin, lipid, and carbohydrate were identified. Humic acid which is the major part of peloid organic matter concentration varied 0.59% and 41.54% of total organic matter of peloid. Composition of organic matter in peloid is to affect its chemical and physical property and to empower bio-chemical process in mud. Holboolj Lake belongs to continental hydrosulfide peloid. Keywords: peloid, hydrosulfide, mineral INTRODUCTION Holboolj Lake and to provide some fundamental P eloids are natural great product and multi- component system, which consists of minerals, water, salt, free and linked organic matter and organo-mineral complex, micro and macro elements, macromolecular data which are useful to explain the therapeutic effects of peloid. EXPERIMENTAL Sampling area. We selected and collected the compounds. Peloids formed a very long period samples of the peloid from the bottom of time by chemical, physical, biological and sediments of the Holboolj Lake that located at geological processes, and that became 1330 m above the sea level and situated healthfulness.The first recorded use of southwest from Bogd sum, Baynkhongor medical mud goes back to ancient Greek but province. The size of the lake is 0.6 km by 1,2 research work of medical mud was not km . Water in this lake is highly mineralized. performed until 19th century. Nowadays, many Methods. For investigation we have used MNS country studied chemical composition and 5849:2008  standard for determination of properties of own country’s peloid. For general characteristics of peloid and organic example, Russian scientists carried out matters isolated from dried peloids, that extensive research on the chemical nature and preliminary washed by distilled water with the therapeutic properties of the sapropel and peat. purpose of cleaning from salts, consistently There are more than hundred lakes with medical extracted with hexane, chloroform, acetone, and mud in Mongolian but we have studied mixture of ethanol: benzene (1:9). The physicochemical properties and some organic concentration of total organic carbon (TOC) compound of healing mud, which is collected was determined using rapid dichromate from 30 lakes. The main objective of this paper oxidation of organic matter (Nelson and is to determine the chemical, physical Sommers, 1996). The composition of organic composition of natural healing mud from compounds was determined by IR method. IR 55 Investigation on characterization and liquefaction of coals from Tsaidamnuur and Khoot deposits S.Batbileg , Ya.Dabaajav, B.Purevsuren, J.Namkhainorov Institiute of Chemistry and Chemical Technology, MAS ABSTRACT: On the basis of proximate, ultimate, petrographic and IR analysis results have been confirmed that the Khoot coal is a middle-rank D mark stone coal and Tsaidamnuur coal is a low-rank B mark lignite brown coal. The results of X-ray fluorescence analysis of coal ash show that the Khoot and Tsaidamnuur coals are subbituminous coal. The ash of Khoot coal has an acidic character and the ash of Tsaidamnuur coal has an alkaline character. The results of pyrolysis of Khoot coal at different heating temperatures show that a maximum yield-10,3% of liquid product can be obtained at 600oC. Also the results of pyrolysis of Tsaidamnuur coal at different heating temperatures show that a maximum yield-13,7% of liquid product can be obtained at 500oC. The results of thermal dissolution of Khoot coal in tetralin with constant mass ratio between coal and tetralin (1:1,8) at 450oC show that 60,8% of liquid product can be obtained after thermal decomposition of the coal organic mass. Also the results of thermal dissolution of Tsaidamnuur coal in tetralin with constant mass ratio between coal and tetralin (1:1,8) at 450 oC show that 80,0% of liquid product can be obtained after thermal decomposition of the coal organic mass. Keywords: pyrolysis, petrographic, mineral compounds, thermal dissolution INTRODUCTION coal basin (Jurassic origin), which contains the C oal has been believed to be the major Baganuur, Ovdogkhudag, Aduunchuluun, energy source and feedstock of chemical Tevshiin govi, Khoot, Tsaidam nuur and Shivee industry among fossil resources in the ovoo deposits and this is located in the central coming century because of its abundant economic region of Mongolia [1 ]. The most reserves and easy availability. Because of important features of these deposits are accessed instability on world oil market , the by opencast mining and coal can be transported diversification of energy carriers is practically using the nearby railway. In Mongolia coal is implemented in many countries with currently the main energy carrier for thermal involvement of various nontraditional types of power plants and local boiler houses and there is organic raw materials, primarily, coal, whose almost no other form of large-scale coal reserves are much greater than oil and gas utilization industry [2 ]. Now Mongolia exports reserves. Mongolia is the country of lack of oil about 15 million tons raw coal by trucks from source with relative rich in coal resource. the South gobi to China. However, coal samples Mongolia has 20 billion tons of proven coal from the Tavan tolgoi deposit have been reserves and estimated resources totalling 163 assessed for benefication  and coke billion tons, mostly of them is low-rank brown production [4 ], samples from Baganuur, coal, but remain undeveloped due to a lack of Bayanteeg and Shivee ovoo deposits as fuel for infrastructure. Such reserves include the huge pyrolysis , hydrogenation and gasification Tavan tolgoi deposit in the South Gobi, which [7,8]. Also samples from Ovdogkhudag and contains over 5 billion tons of high quality stone Aduunchuluun deposits have been assessed for and coking coal, but lies more than 400 km their liquefaction potential using facilities in from the nearest railway. There is a large brown Japan . However, the coals of Khoot and 58 Investigation of Phenolic Compounds of Scabiosa comosa, structure elucidation of cosmosine D.Batsuren1, A.Tuvshintugs1, B.Ganpurev2, B.Sodbayar2, B.Enkhjargal1, J.Tunsag1 1 Institute of Chemistry and Chemical Technology, MAS, 2 Health Sciences University of Mongolia e-mail: email@example.com ABSTRACT: From the areal parts of Scabiosa comosa Fisch. has been isolated cosmosine. The structure was confirmed by chemical and spectral analysis. Keywords: Scabiosa comosa, flavonoid, cosmosine, phenolic compounds, diethyl ester of caffeic acid, apigenin, apigenin-O-glycoside, NMR INTRODUCTION EXPERIMENTAL I n traditional Mongolian medicine aerial parts of Scabiosa comosa used for treatment of diseases of urino-genital system and kidney diseases. From 88g ethylacetate fraction of the aerial parts of Scabiosa comosa For structure elucidation of compounds we used 1 H, 13C NMR, DEPT, two dimensional (H-H- 2D COSY HSQC HMBC) spectral methods. Spectra recorded on BRUKER 500MHz NMR spectrometer. Solvent dimethyl sulfoxide isolated biologically active compound which (DMSO) is used. Chemical shifts expressed has yellow colour. This compound was with ppm, spin-spin coupling – Hz, the quantity crystallized by chloroform-acetone mixture and of protons calculated by integral curve. filtrated, after drying we obtained 5,9mg For structure elucidation of the compound S-2 compound (S-2) belonging to flavonoid group. we dissolved it in DMSO and recorded their 1H Compound S-2 separated by using of NMR spectra (Pig.1). chloroform-methanol (98:2) system as eluent. According to 1H NMR spectra showed the This work is the continuation of previous work presence of two protons at chemical shift 7.96 [2,3] concerning to isolation of caffeic acid’s ppm (2H, d, J=8Hz) and 6.93 ppm (2H, d, diethyl ether and apigenin from Scabiosa J=8,5Hz), as well as two signals of aromatics comosa. In papers [2,3] described this plant, protons with J=8Hz. Thus, compound S-2 might date of collection, methods for isolation of be included to flavones, 4’ position of C-cycle compounds as well as chromatographical substituted by functional groups. Because of methods. This paper contain material influence of substituted groups protons H-2’, H- concerning to isolation of S-2 (flavon 6’, H-3’, H-5’ transfer to magnitude equivalent compound 7-O--D-glycopyranoside) and condition and detected signal of those two structure elucidation by 1H, 13C NMR and two proton. This is common properly of two dimensional spectral methods. substituted aromatic protons and referred to 66 Phytochemical constituents on leaves of Mongolian Sea buckthorn (Hippophae rhamnoides L.) G.Davaakhuu1, N.Tsevegsuren1, Ts.Bulgan2 1 School of Chemistry and Chemical Engineering, NUM, Ulaanbaatar, Mongolia 2 School of Technology MUST, Darkhan, Mongolia ABSTRACT: Sea buckthorn (H.rhamnoids L.), a member of the family Elaeagnaceae, is a deciduous spiny shrub or small tree and widely distributed in cold regions of Mongolia such as Khangai, Mongol Daguur, Khovd, Mongol Altai, Gobi Altai and Great Lakes regions. They are rich source of vitamins (especially vitamin C), sugars, organic acids and bioactive compounds. We have conducted biochemical and phytochemical studies of leaves Mongolian Sea buckthorn varieties (Ayaganga, B-32, and Prevoskhodnaya). The contents of flavonoids and proteins in Mongolian sea buckthorn leaves were ranged 0.29-0.35%, 16.43-19.50%. We conclude that leaves of Sea buckthorn growing in Mongolia contain similar biologically active substances and vitamin C (110.8-154.3 mg%) like berries and might be serve as raw material for producing of essential dietary products. Keywords: Sea buckthorn, H.rhamnoids L., Elaeagnaceae INTRODUCTION isoprenols (Salenko et al, 1986; Uransanaa et al, S ea buckthorn (H.rhamnoids L.), a member 2003; Tiffany et al, 2005; Gupta et al., 2005; Zu of the family Elaeagnaceae, is a et al., 2006; Ranjith, 2009; Efterpi, 2012). The deciduous spiny shrub or small tree plant has been used extensively in oriental between 2-4 m high, hard, drought and usually traditional system of medicine for treatment of cold tolerant, useful for the land reclamation asthma, skin diseases, gastric ulcers and lung and farmstead protection (Ligaa et al, 2005; disorders (Ligaa et al, 2005; Ranjith, 2009). Camelia et al, 2008). Sea buckthorn (SBT) is Besides the berries and seeds, leaves of Sea widely distributed in cold regions of Mongolia buckthorn have also been reported as having such as Khangai, Mongol Daguur, Khovd, nutritional properties and have been used in Mongol Altai, Gobi Altai and Great Lakes some countries to produce leaf extract, tea, tea regions (Juuperelmaa, 2011). In Mongolia SBT powder, animal feed, pharmaceuticals and grows approximately 4000 hector area and cosmetics (Tiffany et al, 2005; Suryakumar and covers major part of the forest area in the region Gupta, 2011; Efterpi, 2012;). The phenolic and is being used as food and pharmaceutical compounds found in the leaves have been applications (Avdai, 2012). reported as useful antioxidants (Bandoniene et All parts of Sea buckthorn are considered to be al, 2000; Vaher and Koel, 2003). The rich source of a large number of bioactive phytochemical information of Mongolian Sea substances like flavonoids, carotenoids, buckthorn leaves is scarce in the literature. In vitamins (C, E, K), tannins, phenols, this study were investigated in detail for triterpenoids, glycerides, proteins, some phytochemical composition and antioxidant essential amino acids, minerals (Ca, Mg and K), properties of Mongolian Sea buckthorn folic acid, free and esterified sterols and varieties. 72 Phytochemical and pharmacological studies on the traditional Mongolian drug Garidi-5 E.Sugarjav1, B.Uuganbayar1, G.Davaakhuu2, Z.Oyun1, P.Molor-Erdene3, Z.Ariunaa1, Ch.Chimedragchaa1, S.Oldokh3 1 Traditional Medical Science, Technology and Production Corporation 2 School of Chemistry, Chemical Engineering,NUM, 3 Health Sciences University of Mongolia e-mail: firstname.lastname@example.org ABSTRACT: Garidi-5, a traditional medicine composed of 5 herbs including Terminalia chebula Retz., Aconitum Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus moschiferus, has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. Bioactive compounds of Garidi-5 were determined by the Folin–Ciocalteu method. An antioxidant potential of the methanol extract of Garidi-5 was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay. Writhing was induced in mice by an intraperitoneal injection of 0.6 % acetic acid solution (10 ml/kg). Water extract of Garidi-5 was given orally at concentrations of 20 mg/kg, 80 mg/kg and 200 mg/kg. Aspirin was used as a standard medicine (100 mg/kg). Gallic acid, α-azarone, costunolide, alkaloids and testosterone were detected in Garidi-5. Methanol extract of Garidi-5 showed the highest (95.11%) DPPH radical scavenging activity at concentration of 0.05 µg/ml. Moreover, Garidi-5 significantly reduced the number of writhes induced by acetic acid in mice by 40.4-47.9% suggesting that it has peripheral antinociceptive effect. Keywords: Garidi-5, antioxidant, antinociceptive effect INTRODUCTION is rich in sesquiterpenoid lactones and G aridi-5 has been used in traditional terpenoids . In the present study, Mongolian medicine as an antibacterial antioxidative and antinociceptive activities of and analgesic agent for treatments of Garidi-5 were examined. Phytochemical various diseases including typhus, dyphteria, screening was also performed to determine the joint conditions, neurological and skin presence of some bioactive components disorders. Garidi-5 is composed of 5 herbs including gallic acid, total alkaloids, α-azarone, including Terminalia chebula Retz., Aconitum costunolide, and testosterone. Kusnezoffii Reichb., Acorus calamus L., Saussurea lappa L., and musk of Moschus EXPERIMENTAL moschiferus [1,2]. As written in traditional Animals. Specific pathogen-free white mice medical sourcebooks the compounds of Gairidi- weighing 20-30 g were used for the study and 5 mainly balance humors, detoxify the body, all were housed in a quiet room with 12 h kill bacteria and relieve pain . A variety of light/dark cycle. The study protocol was bioactive substances have been detected in the approved by the Ethical Committee of the components of Garidi-5. Terfalvin B and gallic Health Sciences University of Mongolia and the acid have been found in Terminalia chebula care and handling of animals were in Retz. . Diterpene alkaloids [4,5,6] and accordance with the principles of the Helsinki polysaccharides have been isolated from the Declaration. root of Terminalia chebula. Saussurea lappa L. 75 Preliminary results on characterization of various coal combustion products from Mongolian thermal power stations and their application for preparation of geopolymers A.Minjigmaa, Ts.Zolzaya, B.Davaabal, U.Bayarzul, J.Temuujin Institute of Chemistry and Chemical Technology, MAS ABSTRACT: At present, over 90% of Mongolian electricity is produced by combined heat and thermal power stations. The main coal deposits used for Central power system's power stations are Baganuur, Shivee-ovoo lignite type coal and Shariin gol bituminous coal. In Mongolia almost 600,000 tonnes of coal combustion by-products are disposed of in waste ponds. In this paper we report preliminary characterisation of Mongolian coal combustion by-products including fly ashes sourced directly from thermal power stations and ash retrieved from ponds. It was determined that fly ashes generated from Baganuur and Shivee-ovoo coal deposits are class C, while ash from Shariin gol coal is class F (according the ASTM C618). All ash products were characterised by chemical, mineralogical, granulometrical, porosity, FE-SEM and radiation methods. Fly ashes sourced directly from power stations were found to be more suitable for manufacture of geopolymers for building materials application. Keywords: coal, coal combustion by-products, synthesis, geopolymers INTRODUCTION million tonnes. Ash products are usually T he most common usage of coal is burning as a solid fuel to produce electricity. It is estimated that around 40% of total electricity produced in the world is generated by coal burning disposed of in landfill or in ash ponds, but with such enormous volumes it is creating environmental problems and becoming costly overhead for coal fired power generation. Thus, there is a strong incentive to find useful and (http://en.wikipedia.org/wiki/Coal) and this is sustainable ways to utilise ash products (Wang likely not to be decreased by near future. and Wu, 2006). Currently worldwide utilisation Approximately 10-30% of the original coal is is about 16% of total the total ash produced, not consumed in the burning process and though it varies from country to country. For remains as coal combustion by-products. instance, Japan utilises over 97% of coal ash Generally, the chemical, mineralogical (http://www.jcoal.or.jp/coaltech_en/coalash/ash composition and volume of the coal combustion 01e.html) while in Australia over 40% of the by-products are determined by the content and ash isutilised composition of the inorganic constituent of the (http://www.adaa.asn.au/documents/ADAA_Ms original coal and design and operation of the hip_Report_2010.pdf). Recent reports have power station (Jha et al., 2008). Coal indicated that Mongolia has total coal reserves combustion by-products also can be divided into of over 150 billion tonnes located in more than different types; namely bottom ash or boiler 200 coal deposits within 12 coal basins slag, fly ash and flue gas desulphurisation (http://www.mmre.energy.mn/branch/now/103/ materials. In a review on fly ash utilisation detail/1004090003/). In Mongolia over 90% of Ahmaruzzaman (2010) states that the world's electricity is produced by coal fired power current annual production of coal ash is around stations. The maximum energy production of all 600 million tonnes of which fly ash is about 500 the power stations is 4,256 million kWh, 78 Protein and amino acid compositions in some Mongolian wood-rotting fungi Sh.Naranmandakh School of Biology and Biotechnology, NUM e-mail: email@example.com ABSTRACT: In this study the contents of total nitrogen, crude protein, easy extracted protein and the compositions of free and protein’s amino acids in five wood-rotting fungi, growing in Mongolia were analyzed. All those fungi are medicinal and the medicinal use of them has a very long tradition in the Asian countries. Dried wood-rotting fungi Fomitopsis officinalis (Vill. ex Fr.) and Inonotus obliquus (Pers. ex Fr.) contain very low crude protein (8.06% and 9.12%, respectively) in comparison with edible mushrooms. The protein content of the other 3 species Fomes fomentarus (L. ex Fr.), Fomitopsis pinicola (Schwarts. ex Fr.) and Laetiporus sulphureus (Bull. ex Fr.) were range from 20.79 to 28.31% such as in most medicinal mushrooms. All investigated fungi are a good source of brain amino acids as aspartic and glutamic acids. A high amount of essential free amino acids such as tryptophan, cysteine and methionine was determined in all investigated wood-rotting fungi although some of them showed a low content of protein. Keywords: medicinal fungi, nitrogen content, protein, essential amino acids INTRODUCTION F ungi have influenced human affairs for (Hobbs, 1995). Mushrooms are considered to be thousands of years, either as a direct food a good source of digestible protein, with protein source and in a food process, or as a content greater than most vegetables, but medicine. The nutritive and medicinal values of somewhat less than meat and milk. The protein mushrooms have been recognized all over the content in mushrooms can vary between 10- world. Mushrooms and primarily 40% by dry mass. As well, mushrooms contain basidiomycetes contain a high amount of all essential amino acids: however, hold limited proteins, essential amino acids, minerals, amounts of the sulphur-containing amino acids vitamins and fibers (Chang, 1996). Some of such as cysteine and methionine (Breene, 1990). them produce substances having potential In Mongolia many species of basidiomycetes medical effects, are called medicinal are growing, which are widely used in mushrooms. Researchers suggested that they traditional medicine. In Mongolian tradition a use for treatment of certain types of cancer, wood-rotting basidiomycetes mainly used as a boost the immune system and reduce the risk of tea. According on this reason we have chosen coronary heart disease, because some of the wood-rotting fungi. Main biological active edible mushroom species possess compositions of medicinal fungi are proteins pharmacological properties (Kalac, 2009). and amino acids. But proteins and amino acid’s Mushrooms are low in calories, lack cholesterol composition of Mongolian medicinal fungi have and virtually do not contain fat and sodium. never been investigated up to now. Selenium and niacin that play important role in The aim of the present study was to determine the immune system, thyroid system and the the contents of proteins and composition of the male reproductive system and cancer prevention free and protein amino acids in five wood- are sufficiently contained in mushrooms rotting fungi, growing in Mongolia. 82 Removal characteristics of arsenic by Fe-Mn layered double hydroxide and double oxide E.Otgonjargal1, Kitae Baek2, Ts.Batjargal3, Jung-Seok Yang4 1 Institute of Chemistry and Chemical Technology, MAS 2 Department of Environment Engineering, Chonbuk National University, Republic of Korea 3 Ministry of Environmental and Green Development, Mongolia 4 KIST-Gangneung Institute, Republic of Korea e-mail: firstname.lastname@example.org ABSTRACT: In this study, Fe-Mn-CO3 layered double hydroxides (Fe-Mn LDHs) and Fe-Mn double oxide (Fe-Mn DOs) were prepared by the co-precipitation methods to remove arsenite and arsenate. The physico-chemical characteristics of adsorbents were analyzed by X-ray diffraction, X-ray photoelectron spectrum, Fourier transform infrared spectroscopy and scanning electron microscopy. The adsorption characteristics of arsenite and arsenate were investigated in points of adsorption kinetics, isotherms, and thermodynamics usaing batch and column experiments. The adsorption of As(III) onto Fe-Mn LDHs and Fe-Mn DOs were occurred five times faster than that of As(V), where pseudo-second order model was well fitted with the experimental data compared to other kinetic models. The adsorption capacity of arsenic from solution by Fe-Mn LDH and Fe-Mn DOs were more fitted to both the Langmuir and Freundlich isotherms compared to Temkin and Dubinin-Radushkevich adsorption isotherm models. The adsorption of arsenic onto the Fe-Mn LDH and Fe-Mn DOs increased with the decrease in pH because of more positive charge of surface due to protonation.The results indicate that the Fe-Mn LDHs and Fe-Mn DOs are a novel and excellent adsorbents for the removal of arsenic. Keywords: Arsenite, Arsenate, Layered Double oxide, Double oxide, Adsorption INTRODUCTION it is difficult to remove As(III) by adsorption A rsenic (As) pollution in drinking or processes, and, therefore, As(III) requires surface water has become a worldwide oxidation before adsorption. environmental issue and is a serious Layered double hydroxides (LDHs) are two- threat to human health. In many countries, dimensional nanostructured anionic clays found numerous people have been made ill by arsenic- in naturally occurring minerals. Generally, contaminated groundwater. In 2006, the World LDHs contain divalent, trivalent, or tetravalent Health Organization (WHO) lowered the metal cations and hydroxyl groups in octahedral standard for As in drinking water to 0.01 mg/L sheets, and oxyanions or water molecules are . In nature, As exists as the inorganic imported into the interlayer region between the oxyanionic forms As(V) and As(III): As(III) is sheets[4, 5]. Many research groups have more toxic than As(V), and its removal is more reported the potential of LDHs to remove difficult . A common choice to remove toxic inorganic contaminants such as oxyanions and compounds from aqueous streams is the process mono-atomic anions from aqueous solutions by of adsorption. As(V) can be easily removed by the processes of adsorption and ion exchange. adsorption technology using adsorbents Recently, great interests on Fe-Mn oxide have including iron oxyhydroxide, activated been increasing for arsenic removal. alumina, and steel-making slags . However, Chakravarty et al.  and Deschamps et al.  87 Relationship between structure and thermodynamic activity of carbon black A.M.Amdur1, V.V.Pavlov1, B.Purevsuren2, L.Munkhtuul1 1 Ural State Mining University, Yekaterinburg, Russia 2 -Institute of Chemistry and Chemical Technology, MAS, Ulaanbaatar, Mongolia ABSTRACT: The role of carbon black is especially important in cokeless metallurgy. Carbon black can be isolated at less hot zones (less than 720oC) in metallurgical ovens according to equation of Buduara: 2CO = C +CO 2. The particles of carbon black obtained by the reaction of Buduara are characterizing with complicated open-work structure including globular amorphous parts and graphitized crystalline elements connected by crosspieses with size in nanometric range ( from 0,1 µm to 3µm). The carbon black is characterizing with increased Gibbs’s energy and high kinetic activity because of its dispersed and amorphous structure. Keywords: carbon, carbon black, reduction reaction, globular amorphous INTRODUCTION - r after which a new surface SV will be created, a work A=σSV will be done, and the thermodynamic C arbon black is a more active reductor in metallurgical process in comparison with potential (G) will be increased by the same value other solid fuels. It can be isolated at less hot ΔG o = A = σSV. If we accept that the particles have zones (less than 720 oC) in metallurgical ovens spherical form, then specific surface S is equal to according to equation of Buduara . their surface 4рr2, divided onto the volume V=( 4рr3)/3, S = (4рr2) / ( 4рr3)/3 =3/r, then 2CO = C +CO2 (1) ΔGo =A=σ S V = 3σM/dr (2) The role of carbon black is especially important in cokeless metallurgy. The content of carbon in a The pressure P of steam, thermodynamic activity metalized pellets (briquette) should correspond to and constant K of reaction equilibrium, in which the mark of smelting steel. It can be regulated by participate dispersed substance depends from the saturation of spongy iron carbon with carbon black, potential G0 by exponent. Therefore increasing the being a product of methane decomposition in a potential Go of liquid by the value (2) brings to cooler zone of shaft furnace. The important growth of the pressure P of steam and constant K in advantage of carbon black in comparison with other exp (3 σM/d r RT) once in comparison with the solid fuels consists in the fact that it is isolated or value for weight of substances P0, K0: formed from the gas phase and therefore does not contain ash and other admixtures of ordinary fuel. P / P0= ехр(3σМ / d r RT); ( 3 ) Increased thermodynamic and kinetic activity of К / К0 = ехр(3σМ / d r RT). carbon black is based on its high dispersion, highly developed reaction surface and also by unstable Therefore for example the pressure of water steam internal structure of the atomic packing, often increases when the size of water drops decrease. having not crystalline but amorphous structure. Lets to see a mist consists of nano-drops of water Let’s consider the increase of thermodynamic with radius 1 nanometer. The tension of water σ =73 activity after dispersing. If there is a mole of the mJ/m2, molecular weight M = 18 10-3 kg/mole, substance with a volume V=M/d (M-molecular density d=1000 kg/м3. So for such drops with radius weight, d-density) pound onto particles with radius 98 Some phytochemical constituents and bioactivity of water and ethanolic extract of Rumex acetosella L. D.Punsaldulam1, E.Munkhbat2, D.Otgonsuren2 1 Institute of Botany, MAS, 2 Institute of Chemistry and Chemical Technology, MAS e-mail: email@example.com ABSTRACT: Total flavonoids, anthocyanins and hydrolysable tannin’s contents were determined in water and alcoholic extract in different parts of Rumex acetosella L. Antioxidant assays and brine shrimp cytotoxicity assay were followed. Maximum amount of flavonoid (59.67±0.4 mg/g) determined in leaf’s ethanolic extract, the hydrolyzable tannins - in root’s ethanolic extract (94.78±1.45 mg/g) and the anthocyanins (0.14%) in flower’s ethanolic extract. Maximum correlation occurred between hydrolyzable tannins and reducing power or antioxidant capacity (p = 0.001, R2 = 65.5% and 85.8%, p = 0.001, respectively). In preliminary result of brine shrimp assay, significant deaths of nauplii were observed only in root extracts (LD50≈160µg/ml in ethanol and≈1000 µg/ml in water extract). Keywords: flavonoids, anthocyanins, condensed tannins, brine shrimp assay INTRODUCTION reveal anticancer compound - taxol, which is R umex are plants distributed widely in Mongolia. Totally, 13 species are found. Some of these species are used in traditional medicine to treat several unhealthy conditions (1). Rumex plants are reported to used in chemotherapy against cancer (7). EXPERIMENTAL Plant materials were collected in June, 2012 in flowering stage from Batnorov sum, Dornod contain anthraquinones, flavonoids (2,3), province. After collection, plants were separated naphthalenes (4), proanthocyanidins (5,6), into flower, leaf, stem and root and air dried at phloroglucinol (6). R. acetosa L. (2), room temperature. Extraction was done in three R.nepalensis L. (4) showed cytotoxic activity. different conditions: 1 hour in distilled water at However, there is no enough scientific boiling water bath, 48 hours in distilled water information about phytochemicals and their and in 70% ethanol. Phytochemicals and bioactivity of R.acetosella L. A medicinal plant bioactivity assays were conducted in these book said that this plant had purgative, diuretic, extracts. Brine shrimp cyst was purchased from antibacterial and wounds repairing activity (1). local fish store and kept in dark, cool place. All Therefore, we aimed at investigating some chemicals used were analytical grade. phenolics of R.acetosella L. and their Spectrophotometric determination of total relationship with antioxidant capacity. Many flavonoids. Quantitative determination of biological activities such as antioxidant activity flavonoids in water and ethanol extract of are attributed to plant phenolics. Also, possible different parts of plants were carried out using usage of polyphenolics as remedy for cancer aluminium chloride spectrophotometric method treatment is extensively studied recently. In this described by Ruch with slight modifications study, brine shrimp cytotoxicity assay was used (8,9). In short, 200µl of previously prepared to assess possible cytotoxic effect. According plant extracted were mixed with same amount to J.B. Harborn (1998), this test was used to of AlCl3 in ethanol and final volume was made 102 Synthesis and their photodynamic activity of new photosensitizers for PDT B.Bayarmaa1, Young Key Shim2 1 Institute of Chemistry and Chemical Technology of MAS, Ulaanbaatar Mongolia, 2 PDT Research Institute, School of Nano Systems Engineering, Inje University, Gimhae, Republic of Korea e-mail: firstname.lastname@example.org ABSTRACT: The main goal of this research is to synthesize new candidates of chlorin based photosensitizers and to evaluate their the antitumor photodynamic effect. In this research we used A549 (human lung carcinoma cell). The cytotoxic activity was measured by microculture tetrazolium (MTT) assay, and morphological changes induced by PDT were checked by microscopy. The cytotoxic effect of photosensitizers was shown to increase depending upon the concentration of photosensitizers. New chlorin derivative photosensitizers were low toxic to tumor cells in the absence of light, while there was remarkable tumor cell death after photodynamic therapy. Keywords: photodynamic therapy, photosensitizer, tropolone, lung cancer cell INTRODUCTION particular substituents on the chlorin parent ring P hotodynamic therapy (PDT) is relatively new method for the treatment of cancerous and nonmalignant tumors using a combination of visible or near-infrared light and a ligth -activated drug, or may have a large influence on the photosensiting properties of the compound when used in PDT treatments. Chlorin-based photosensitizers, with a variety of substituents on the periphery of the parent ring, photosensitizer [1,2]. The photosensitizer is have been synthesized and exhibit strong activated by light to produce a cytotoxic absorption in the red visible region. This strong species, which in turn promotes the destruction absorption allows for deeper light penetration of the target tissue . and therefore higher efficiency for singlet In the presence of molecular oxygen, the oxygen generation. In addition, the ease with photosensitizer elicits a photochemical reaction which these compounds can be chemically resulting in the production of highly a reactive modified allows tailoring of properties such as species, particularly singlet oxygen , which is aggregation and cellular uptake. toxic to the cells in which it is produced. The Modification of the β-position on the porphyrin behavior of the photosensitizer is thus or chlorin ring increases the degree of cell paramount to understanding, and improving, the penetration and affords many advantageous efficacy of the PDT-based treatments. Emphasis pharmacological properties. Seven-membered in the development of new photosensitizers has cyclic compounds linked to a chlorin skeleton, been placed primarily on the natural chlorins, such as 2-hydroxy-2,4,6-cycloheptatriene-1- which differ markedly from symmetric one, also known as tropolone (TRN), may porphyrins, the former having substantially exhibit several pharmacological advantages. stabilized S1-energies, strong Q-absorption The chemical structure of tropolone has been bands, and unique redox reactivities. Previous known since 1945, and contains aromatic reports  suggest that the placement of features . Lung cancer are a frequent cause of 108 Synthesis and catalytic test of bimetallic Pd-Ag nanoparticle prepared by the impregnation method Sh.Nyamdelger1, G.Burmaa1,Christian Weilach2 and Karin F ttinger2 1 Institute of Chemistry and Chemical Technology, MAS, Ulaanbaatar, Mongolia 2 Institute of Materials Chemistry, Vienna University of Technology, Austria e-mail:email@example.com ABSTRACT: We have synthesized mono and bimetallic catalysts (Pd,Ag, Pd-Ag, Ag-Pd) supported on γ-Al2O3 by the sequential impregnation method. The bimetallic catalysts were prepared with a fixed Pd loading (4 wt.%) by incipient impregnation of one metal precursor and calcination, followed by the impregnation of the second metal precursor and a second calcinations step. Obtained materials were characterized by TEM, XRD and IRAS and catalytic test measurements. The temperature programmed CO oxidation and IR results showed that the bimetallic catalysts consisted of alloyed nanoparticles and indicated that the surface mainly consists of isolated Pd atoms in a Ag matrix. The catalytic activity of the catalysts for hydrodechlorination of TCE was tested. For the pure Pd/γ- Al2O3 catalyst, the catalytic activity was observed in the hydrodechlorination reaction of TCE. The catalytic performance of the bimetallic samples was poor under these conditions, but may be increased by changing the Pd/Ag ratio or the H2/TCE ratio e.g. during reaction. Keywords: nanoparticles; bimetallic catalysts; impregnation method; hydrodechlorination INTRODUCTION waste [Gentsler,2008]. These are: E nvironmental pollution polychlorinated aromatic (PCA) and aliphatic (PCAl) species is of great concern today. Such compounds are distributed by widely throughout the world due to their diverse 1. Hydrogenation leads to complete conversion of original organic compound to its non harmful derivative in mild condition 2. Selective reaction take place during recycling allowing dechlorination of use. Such compounds can be released into chlorinated organic compounds of different water, soil and air from household or concentration. industrial facilities. The options of their 3. During hydrogenation no secondary toxic disposal related to incineration, pyrolysis, compounds (e.g. chlorine NOx, phosgene biological treatment and catalytic oxidation are and dioxin) will be produced associated with drawbacks of high toxic 4. No energy needed during catalytic emissions such as dioxins, low rate of reaction convertion and high energy cost [Beteley, 2011]. The most For the establishment of method for challenging method for recycling and hydrogenation of chloroaromatic compounds, dechlorination of such compound is conversion an establishment of catalytic system fulfilling to ecologically friendly derivatives. On the other condition for stable catalysator with high hand, catalytic dechlorination and activity is of utmost importance. Noble metals hydrogenation are methods of choice for are very active for the hydrodechlorination of detoxication and recycling of halogenorganic chlorine organic compounds producing fully 114 Spectroscopic study and implications for biological reactive oxygen space sensing of the inorganic DMSO/POCl3 reaction with BODIPY N.Amgalan1, 2, Taehong Jun2, Kang Mun Lee2, HyuDavid G.Churchill2 1 Department of General and Analytical Chemistry, School of Chemistry and Chemical Engineering, NUM, Ulaanbaatar, Mongolia, 2 Molecular Logic gate Laboratory, Department of Chemistry, KAIST, Republic of Korea e-mail: firstname.lastname@example.org ABSTRACT: The reaction of boron dipyrromethene (BODIPY) species with DMSO and POCl 3 to afford the 2- chloro,6-methylsulfido derivatives . Systems underwent reaction to give the 4,4-difluoro-2-chloro-6-methylsulfido-8- (C4H3X)-4-bora-3a,4a-diaza-s-indacene (Compounds 2a-2d). The species were characterized completely by NMR (1H, 13 C) and X-ray diffraction. Keywords: BODIPY; Sulfido; Sulfur oxidation; Fluorescence; DMSO. INTRODUCTION state increases; subsequent fluorescence or B asic fluorescent properties of fluorescent colorimetric changes may ensure. The novel 4- probe are heavily dependent on the difluoro-2-chloro-6-methylsulfido-1,3,5,7-8-phenyl- fluorophore, that is selected as the main 4-bora-3a,4a-diaza-s-indacene was synthesized with skeleton of the fluorescent probe.In our case, the DMSO/POCl3. choice of difluoroboradiaza-s-indacenes, commonly denoted boron-dipyrromethene dyes (F- EXPERIMENTAL BODIPY)reflects the thinking that this class of General Considerations. All chemicals used herein compounds would act as a very efficient fluorescent were used as received from commercial suppliers label and that the surrounding decoration might act (Aldrich, Acros, and Junsei companies). The as the recognition sites for many different analytes. synthetic details for the preparation of the Furthermore, the optical properties can be tuned to dipyrromethanes and for the BODIPY systems an incredible large extended by modifying the follow literature methods .1H and 13C NMR pyrrole core,the central meso-position and the boron spectra were acquired using a BrukerAvance 300 or substituents. 400 MHz spectrometer. TMS was used as an BODIPY is a fluorescent probe for indexing lipid internal standard. 1H and 13C NMR spectral signals peroxidation and antioxidant efficacy in model were calibrated internally by the respective protio membrane systems and living cells, with excellent impurity or carbon resonance of the CDCl 3 (1H characteristics; emission in the visible range of the NMR: δ 7.24; 13C NMR: δ 77.0) or CD2Cl2 solvent electromagnetic spectrum, with good spectral (1H: δ 5.32, 13C: δ 53.8). C, H, N elemental separation of the monoxidized (595 nm) and analyses were measured using a Vario EL III CHNS oxidized (520 nm) forms . elemental analyzer. High resolution matrix-assisted Herein, we report the facile synthesis and properties laser desorption/ionization (MALDI) mass of a new BODIPY dye bearing a methylthioether spectrometry was performed on a VG AUTOSPEC group. Compounds 1a, 1b, 1c, and 1d have been ULTIMA by the research support staff at KAIST. previously reported by this research group . This instrument possesses a tri sector double Sulfide substitutions can become chemically focusing magnetic sector analyzer and was operated oxidized species and give rise to discrete valence at a resolution of 80,000. To introduce the desired 120 Study of biological activity compounds in some Mongolian medicinal plants S.Bayaraa, J.Batkhuu, A.Bayanmunkh, L.Khurelbaatar Drug research institute e-mail:email@example.com ABSTRACT: The aim of this study is to investigate biological activity compounds in Mongolian traditional medicinal plants, such as Vaccinium Vitisidaea L., Iris tenufolia Pall., Oxytropis pseudoglandulos and Ribes diacanthum Pall. We were determined total flavonoid, total coumarin, tannin and saponin by method of Mongolian National Pharmacopeia  and antibacterial activity by paper-disk method . Content of biological activite compounds in plant raw material were: 1.25-7.40% of total flavonoids, 2.06-4.13% of total coumarins, 2.13-6.30% of tannins and 2.80-4.71% saponins in plants and 43.43-233.58 mg% of total flavonoids and 4.00-752.66 mg% of total coumarins in extracts of plants. Vaccinium Vitisidaea L., Iris tenufolia Pall. and Oxytropis pseudoglandulos have antibacterial activity. But Ribes diacanthum Pall. was not determine antibacterial acitivity. Keywords: Vaccinium Vitisidaea L., Iris tenufolia Pall., Oxytropis pseudoglandulos, Ribes diacanthum Pall, antibacterial acitivity INTRODUCTION and saponin were determined by method of T he kidney diseases are increasing year by year in our country. In traditional medicine many plants used for treatment of kidney diseases. The biological activity of all compounds and antibacterial activity of the Mongolian National Pharmacopeia, MNS 4163:2009, MNS 4166:1993 and MNS 4441:1997. Vaccinium Vitisidaea L. was extracted with water (1:10), Ribes diacanthum was extracted in water (1:5), Iris tenufolia and extract of Vaccinium Vitisidaea L., root of Iris Oxytropis pseudoglandulos were extracted with tenufolia Pall., herb of Oxytropis ethanol 70%. (1:10). Extract of Iris tenufolilia, pseudoglandulos and Ribes diacanthum Pall Oxytropis pseudoglandulos was concentrated to have been done. In literature, those plants are 1g/ml, 0.5g/ml (respectively). Extracts of rich in phenolic compounds, such as flavonoids Vaccinium Vitisidaea L. and Ribes diacanthum and phenoilic acids, which exhibit wide range of were not concentrated. biological effects including antioxidant and antibacterial activity. RESULTS AND DISCUSSION Content of biological activity compounds in EXPERIMENTAL plants are shown in Table1. Medicinal plants were harvested in 2011 from Bogd-Kan Uul and Chingeltei Uul. We studied Table1. Content of biological activity leaves of Vaccinium Vitisidaea L., roots of Iris compounds in plant raw material tenufolia Pall., herb of Oxytropis pseudoglandulos and herb of Ribes diacanthum Pall. Anticbacterial activity of extracts was evualuated by paper-disk method of Kirby – Bauer .Total flavonoid, total coumarin, tannin 123 Study biological activity of alkaloid protopine S.Javzan 1, Y.Jamyansan1, R.I.Alexandrova2, D.S.Bae3 , Ch.W.Nho4 1 Institute of Chemistry and Chemical Technology, MAS, Ulaanbaatar, Mongolia 2 Institute of Experimental Pathology and Parasitology, BAS, Sofia, Bulgaria 3 Department of Biology Education, College of Education, Chungbuk National University, Cheongju 3 4 Functional food Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea e-mail: S_Javzan@yahoo.com ABSTRACT: Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from H. erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw-264.7cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase- 2 (COX-2) and prostaglandin-E2 (PGE2) production by LPS-stimulated Raw-264.7 cells, without a cytotoxic effect. Pre- treatment of Raw 264.7cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB). Also cytotoxic and antiproliferative activities of the protopine on tumour (LSCC-SF-Ms29, LSR-SF-SR) and nontumour (L 929, MDBK) cells were evaluated. The maximal nontoxic concentrations (MNC) as well as concentrations required to reduce cell viability by 50% (CC50) at 24h and 48h were determined using the trypan blue dye exclusion test. Comparable CC 50 (24h) data were obtained for protopine (25µM; 92 µM) and mytomycin C (24 µM≥30 µM) for LSCC-SF (Ms29) and LSR-SF(SR) cells, respectively. In MNC the alkaloid decreased the proliferation of LSCC-SF-Ms29, MDBK and L929 by 58, 42, 19 and 21%, respectively. The alkaloid was much less toxic and cytostatic for cells from L929 and MDBK as compared to cells from tumour lines. The compound inhibited the colony-forming ability of tumour cells in a dose dependent manner. In doses ≥0.001 µM the protopine blcked the colony-forming capacity of normal murine-marrow cells. Keywords: Hypecoum erectum; protopine, cytotoxic, antiproliferative, inflammation activity INTRODUCTION coridamine, hypecorine, hypecorinine, H ypecoum erectum L. belongs to the family Hypecoaceae in which belongs only one genus. The genus Hypecoum is represented by three species in Mongolian flora. H. erectum is widely spread in berberine) from H.erectum grown in Mongolia, 5 of which named by us as 8-oxyhunnemanine, 8-oxyallocryptopine, tautomer of hyperectine, 8-oxyprotopine A, 8-oxyprotopine B, were found to be as novel natural compounds. Khentei, Khangai, Mongol Daurian, Middle Elucidation of the structures of 8- Khalkha, Mongolian Altai, East Mongolia and oxyhunnemanine, 8-oxyallocryptopine, 8- East Gobi regions . Russian scientist have oxyprotopine C, 8-oxyprotopine D allowed us been found nine isoquinoline alkaloids from to conclude about the existence of a new group H.erectum [2.3], but only protopine has been of naturally occurring 8-oxyprotopine alkaloids isolated the same from species of Mongolian . In the traditional medicine of Tibet and origin . We have been some alkaloids Mongolia the plant is named “bar ba da” and (protopine, allocryptopine, oxyhydrastinine, has been used for many years due for as 125 Study on the washability of Baganuur coal by using float sink test A.Ariunaa1, G.Tsatsral1, J.Narangerel2, R.Erdenechimeg1, J.Dugarjav1, B.Purevsuren1, S.Jargalmaa1 1 Institiute of Chemistry and Chemical Technology MAS, 2 Mining Research Laboratory, MRAM ABSTRACT: The wash ability of coal sample from seam number 4 of Baganuur deposit, which is located in Nyalga- Choir basin, was studied. The proximate and ultimate analyses of the sample were done by standard methods. The sample was crushed and pulverized in order to obtain 3 fractions with particle sizes of 3-1.5mm, 1.5-1.0mm and 1.0- 0.63mm. Heavy liquids were obtained by dissolving different amounts of ZnCl 2 salt in water. The liquids having <1.3; 1.3-1.4; 1.4-1.5; 1.5-1.6; 1.6-1.7; 1.7-1.8 and >1.8g/cm3 densities were used for float sink test of the fractions. Float sink test was done by using Russian standard GOST 4790-93. Results of the proximate and ultimate analyses showed that the coal has Vdaf=47.0%, Ad=13.3%, Cdaf=70.53%. According to the coal classification, the coal belongs to the B2 class of brown coal. Yields and ash contents of the fraction with 3-1.5mm, 1.5-1.0mm particle size were 51.8 and 7.7%, 46.6% and 7.4% respectively, when for the separation was used heavy liquid with 1.4g/cm 3 density. In general, it can be concluded that the Baganuur coal belongs to the category of coals with very poor washability. Keywords: ash, coal washability, float sink test INTRODUCTION geological expedition (B.Chuluun and C oal is the most important mineral L.Monkhtogoo) has estimated total geological resource of Mongolia. Total geological resource of the coal as 713 million tonnes [1,2]. resource of coal in the country has been Proximate analyses. Mongolian National estimated approximately 163 billion tones and Standards (MNS) were used for proximate the number can be increased in future. analyses; moisture (MNS 655-72), ash (MNS Approximately half of this resource is low rank 652-72), volatile matter (MNS 654-72), coal or brown coals. Due to high moisture and calorific value (MNS 669-72), sulphur content ash contents, low rank coals have a limited (MNS 656-72). application in the industrial use, especially in Ultimate analyses. Contents of C, H, N, O gasification and liquefaction plants. Upgrading elements of the sample were determined by of low rank coals is an important step for further using CHN analyser of the Irkutsk Institute of processing of coal. Chemistry, Russia. Purpose of the research was to determine a Float sink test procedure. In laboratory possibility to reduce ash content of Baganuur condition, coal washability is determined by coal through dense medium separation. GOST 4790-93 standard. Fractions with particle sizes of 3-1.5mm, 1.5-1.0mm and 1.0-0.63mm EXPERIMENTAL were separated by float sink test procedure into Baganuur coal deposit, which belongs to the sub-fractions with densities having <1.3; 1.3- Nyalga-Choir basin, is located at 110 km to the 1.4; 1.4-1.5; 1.5-1.6; 1.6-1.7; 1.7-1.8 and south east from Ulaanbaatar in the Central >1.8g/cm3. Heavy liquids were obtained by economic region of Mongolia. In 1988 Central dissolving different amounts of ZnCl2 salt in 134 Study on biological activity of Hyoscyamus niger L. grown in Mongolia J.Irekhbayar1, G.Davaakhuu1, S.Odontuya2, Burm-Jong Lee3 1 School of Chemistry and Chemical Engineering, NUM, Mongolia 2 Central Geological Laboratory, Mongolia 3 Department of Biomedical Chemistry, Inje University, Obang-Dong, Gimhae, 621-749, South Korea e-mail: firstname.lastname@example.org ABSTRACT: This study was aimed to evaluate the antioxidant and cytotoxic activities, hepatoprotective effects of the aerial parts of Hyoscyamus niger L. grown in Mongolia. The ethanol crude extract of H.niger L. was suspended in water and consequently fractionated with hexane, chloroform, ethyl acetate and butyl alcohol. All of the fractions were examined for their antioxidant and cytotoxic activities by using DPPH and MTT assays, respectively. Hexane fraction showed the highest DPPH radical scavenging activity at concentration of 50 µg/mL by 87.36% with the IC 50 value of 18.16 µg/mL. MTT results showed that ethyl acetate fraction demonstrated significant cytotoxicity against A549 cell line, with values of 90.47% at 48 h (50 µg /mL). The water fraction notably protected HepG2 cells against the cytotoxicity of t-BHP than EGCG. H.niger L. is an important medicinal plant and reported to be used for the treatment of various ailments. Keywords: Hyoscyamus niger L., antioxidant, cytoxicity INTRODUCTION [3, 4]. This is a very poisonous plant that should T he genus Hyoscyamus belongs to the family Solanaceae, which comprises a group of some 50 species all over the world. One of them (Hyoscyamus niger L.) is distributed in Mongolia [1, 2]. It is used be used with great caution, and only under the supervision of a qualified practitioner [4, 5]. The seeds are used in Tibetan medicine, they are said to have a bitter, acrid taste with a neutral and poisonous potency. Anthelmintic, extensively as a sedative and pain killer and is antitumor and febrifuge, they are used in the specifically used for pain affecting the urinary treatment of stomach/intestinal pain due to tract, especially when due to kidney stones. Its worm infestation, toothache, inflammation of sedative and antispasmodic effect makes it a the pulmonary region and tumours . valuable treatment for the symptoms of To the best of our knowledge, there are no Parkinson's disease, relieving tremor and published reports on the biological activities of rigidity during the early stages of the disease the extracts of H.niger L. grown in Mongolia. . The plant is used internally in the treatment Therefore, the present study was conducted. of asthma, whooping cough, motion sickness, Meniere’s syndrome, tremor in senility or EXPERIMENTAL paralysis and as a pre-operative medication . Plant material. The aerial parts of H.niger L. H.niger L. reduces mucous secretions, as well were gathered from Selenge aimag, Mongolia in as saliva and other digestive juices. Externally, July, 2010. Similarly, soil was taken from the it is used as an oil to relieve painful conditions same place. Voucher specimens have been dried such as neuralgia, dental and rheumatic pains separately in room temperature. 139 Use of modifed zeolites for chromium removal from tannery wastewater D.Batgerel1, S.Erkhembayar2, E.Anudari1, G.Ariunzul3 1 School of Chemistry and Chemical Enjineering, NUM, 2 School of Civil Enjineering and Architecture, MUST, 3 Energy Resources LLC e-mail:email@example.com ABSTRACT: Adsorption of heavy metal ions specifically, Cu (II), Zn (II), As (V) and Cr (VI) from waste water by modified zeolite was evaluated. The modified zeolite was synthesized previously by dehydroxylation of low grade at 450°C and reacted hydrothermally with 2-4 M NaOH solution at 100°C for 2 h. The adsorption capacity decreased in the order Cu(II) > Zn(II) > As(V)>Cr(VI) ions. Almost complete adsorption was achieved for Cr(VI) was 91% and 52.3% at pH 7.0 and 8.4, respectively. The results also showed that the grain size of the modified zeolite has a little effect on the adsorption capacity. The experimental data was compared with two adsorption isotherms, Langmuir and Freundlich isotherms. The experimental results showed a good agreement with the Langmuir plots . Keywords: Chromium, Adsorption, Zeolite, Modification, Tannery Wastewater INTRODUCTION calcining of law grade for 4 h in air at 450°C Z eolites are well known for their ion exchange capacity. The role of zeolites in the conversion of solid and liquid Tannery wastes into environmentally acceptable products has also been demonstrated. Modified and reacted hydrothermally with 2-4 M NaOH solution at 100°C for 2 h. The obtained gel was filtered first, then washed till pH 10, afterwards, the product was dried at 100°C for 24 h. The produced zeolite is characterized as average zeolites are useful because of their controlled particle size of 1-5 mm. and known physico-chemical properties relative Batch Adsorption Experiments. The pH values to that for natural zeolites. The focus of the of the solutions were adjusted from 1 to 11 with present study was evaluation of the 0.5 M NaOH and 0.5 M HClO4 and measured effectiveness of the modified zeolites in their with a pH meter. The pH value of solution was NH4 forms for removal of chromium from water kept at a specific value during the experiments over a wide range of pH. A similar reaction is using a pH controller. The liquid samples were possible for adsorption of chromium onto filtered after adsorption for metal ions analysis zeolites, where terminal aluminol or silanol by FEC. hydroxyl groups develop at the edges of the Adsorption Capacity. The adsorbents (0.25 g) zeolite particle. were left in contact with 100 mL of each of Cr solutions in the range of 50-5 mg L-1 with the EXPERIMENTAL initial pH value of 10 for 30 min. The amount of Adsorbent. Natural zeolite was provided by the metal ions adsorption onto the zeolite can be deposits of Tragabtsav and Urgun, Mongolia. calculated by: Qe=(Ci-Ce)/S where, qe is the The zeolite was synthesized previously by metal ions adsorbed onto the zeolite (mg g -1), Ci 143
"Mongolian Journal of Chemisrty"