LORRE Safety Retraining 2010

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					LORRE Safety Retraining 2010
                 LORRE Safety Committee
                            June 7, 2010
                    Outline
   Significance of safety retraining
   BSL-1, BSL-2 agents used in the lab
   Personal Protective Equipment
   Safe Storage of Acids and Bases
   HPLC Safety
   LORRE Safety Website
   Integrated Safety Plan/Self Audit
   Building Emergency Plan
           Significance of safety retraining
            Everyone has received safety training before so why
              is it important to have annual safety retraining?

   Important to review the original safety rules and safety training information
   Important to learn and put into practice any new information from the retraining
   Important to improve experimental practices to avoid heavy fines or warnings
   Important to keep the consequence of safety fresh in mind to avoid lab related
    injuries
   Important because its is everyone’s responsibility to follow the safety plan it is
    everyone’s right to have a safe working environment

  This year retraining involves BSL-1 and BSL-2 which could be used in the Lab,
corrections from REM inspections, and some new safety situations.
   BSL-1, BSL-2 Agents Used in Labs
 The BioChips Project uses several different types of bacteria
   • This includes Salmonella, E. coli and Listeria monocytogenes
   • People must remember to be careful with the bacteria and take all
     necessary precautions to avoid harm.
 Biosafety level 1
   • BSL-1 is appropriate for working with microorganisms that are not
     known to cause disease in healthy humans. They pose a low risk.
 Biosafety level 2
   • BSL-2 laboratories are ones in which working conditions contain
     agents that pose a moderate risk to personnel and the
     environment. They have the ability to cause disease in humans.
BSL-1, BSL-2 Agents Used in Labs
          (continued)
For both BSL-1 and BSL-2
safety environments it is
necessary to…
Train
Wash hands with soap
Clean the work area with
70% ETOH
Work in labs 335, 359 or 236
All BSL-2 bacteria have the
possibility of forming airborne
infectious materials and
                                  Biohazard Sign
should be used only under the
safety hood in Lab #359
                                Listeria
                             Listeria is a bacterial genus containing seven
                             species
                                  L. grayi
                                  L. innocula
                                  L. ivanovii
                                  L. monocytogenes
                                  L. seeligeri
                                  L. muttayi
                                  L. welshimeri

Our lab could possibly use L. innocula and L. monocytogenes
Classification
    L. monocytogenes is a Gram-positive bacterium
          •BSL-2
    L. innocula is a rod-shaped Gram-positive bacteria
          •It is very similar to the food borne pathogen L. monocytogenes but is
          nonpathogenic in character and therefore has a BSL -1
        Listeria monocytogenes
Listeria is typified by Listeria
monocytogenes
It is one of the most violent food borne
pathogens
It causes the disease listeriosis


                          The U.S. Department of Agriculture's Food
                          Safety and Inspection Service (FSIS) is
                          issuing a public health alert due to
                          illnesses possibly associated with ready-to-
                          eat (RTE) deli meat products that may be
                          contaminated with Listeria monocytogenes.
Listeria monocytogenes




         Video
               Salmonella
 Salmonella is a genus of rod-shaped, gram
  negative bacteria
 Salmonella – The Disease
   • It causes a bacterial infection that is
     passed to humans from animals
   • Agents like Enteritis salmonella cause
     food poisoning in people
 Salmonella bacteria is generally
  categorized as Biosafety Level 1
Salmonella




   Video
                   Escherichia coli




     LORRE Biochip Project used E. coli GFP

E. coli is a Gram-negative rod shaped
bacterium
Most E. coli strains are harmless but
some such as serotype 0157:H7 can
cause serious food poisoning in humans
E. coli has a Biosafety Level of 1
E. coli




 Video
Personnel Protective Equipment (PPE)
When bacteria are handled the following PPE must be worn:
 Lab coats
 Gloves
 Face/eye protection
 Closed toe shoes

When bacteria are handled the following procedure must be followed:
 Operate in appropriate biological fume hood
 Pay close attention when handling sharps
 Wash hands thoroughly after use
 Use 70% ethanol and 1% bleach to clean work area after use
 Close the door and display a risk sign if needed to alert others
 Contaminated items such as gloves or plastic materials must be autoclaved
  before disposal
                         PPE
                               Cap


Goggles




                                     Mask
 Gloves


                                     Coat




          Closed-toe shoes
Biosafety Hood




    LORRE Room 335 Class II Bio Safety
               Cabinet
    Safe Storage: Acids and Bases
 Acids and bases should not be stored or used
  near each other. Store acids and bases in
  separate cabinets.
 Keep acids and solvents in different cabinets
 Make sure all containers are sealed properly
 Make sure all labels are clear and visible for
  quick chemical identification
Safe Storage: Acids and Bases



                   Pay attention not to
                      store acids and
                    bases in the same
                    space as shown in
                     picture to the left.
   Acid   Base
  Safe Storage: Acids and Bases

                                 Label and separate organic
                                  acids and inorganic acids as
                                   shown in the surrounding
                                           pictures.




Organic Acid   Inorganic Acid
     Safe Storage: Acids and Bases
                A few common examples
                       include…

Organic Acid     Inorganic Acid       Bases
 Lactic Acid    Hydrochloric acid   Ammonia
 Acetic Acid    Nitric acid         Sodium Hydroxide
 Oxalic Acid    Phosphoric acid
 Uric Acid      Sulfuric acid
 Formic Acid    Boric acid
 Citric Acid    Hydrofluoric acid
                 Hydrobromic acid
HPLC Safety
   Use the Hazardous Material Pickup
   Request form provided by REM in
   order to get rid of your HPLC waste
   Typical chemicals found in the HPLC
   are organic materials regulated by the
   EPA
       Ex:
            •Acetonitrile
            •Methanol
   Sulfuric Acid also requires a form to be
    turned in.
   Most sulfuric acid mobile phases in
   LORRE are 0.005M H2SO4
             HPLC Safety




                    Cover all containers
                       We have bought
                       specialized caps
                       We also have modified
LORRE HPLC System      caps
                        HPLC Safety




We are contacting REM so that they can help us improve the safety of our
workplace by attaching specialized caps to our waste bottles rather than
                  the modified caps we use currently
LORRE Safety Website

            Within the College of
             Engineering (COE) many
             departments have a
             safety website
              • Includes CHE, CE and
                 AAE and others
            Thank you Keven Gipson
             for designing the LORRE
             safety website
            Developed by LORRE
             Safety Committee
            This is a useful tool and
             resource for safety
             information
LORRE Safety Website




  The new LORRE Safety Website
   Integrated Safety Plan Self Audit
 The ISP self-audit is a very important part of the REM walk-
  though process
 In total there are twenty-four categories that make up the self-
  audit checklist
 Of those twenty-four categories some are relevant to our lab
  such as the PPE, CHP, hazard communication, etc
 Other categories are not utilized in the lab such as laser
  safety and radiation safety.
 This year our checklist had some problems. Some categories
  such as the PPE category were improperly checked ‘NO’
  when they were in use. Please pay attention to correctly
  perform the list or check with someone if you are uncertain.
                           Checklist
               Date   Two people who complete the self-audit


Building and                                    Signature of
Room                                            Supervisor

                                                Person
Type of Room
                                                Responsible
                                                for the Room

                                                     Yes
                                                     No
Building Emergency Plan
             Main Contents
             Plan Development and Validation
             Building Emergency Plan
             Building Information
             Notification Procedure
             Emergency Procedures
             Evacuation
             Shelter in Place

             Every lab has a copy for reference
             Learn the Emergency Plan
             Building Safety Committee
             Evacuation

     Emergency Plan
     The emergency assembly area location for POTR is
     between EE and KNOY on the Northeast side of the
 building (pictured to the left). In case of inclement weather
the lobby area inside KNOY will be utilized (pictured above).
Thank you for
   Safety
 Retraining

				
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